Strategies and tools for preventing neurotoxicity: to test, to predict and how to do it

A change in paradigm is needed in the prevention of toxic effects on the nervous system, moving from its present reliance solely on data from animal testing to a prediction model mostly based on in vitro toxicity testing and in silico modeling. According to the report published by the National Research Council (NRC) of the US National Academies of Science, high-throughput in vitro tests will provide evidence for alterations in"toxicity pathways" as the best possible method of large scale toxicity prediction. The challenges to implement this proposal are enormous, and provide much room for debate. While many efforts address the technical aspects of implementing the vision, many questions around it need also to be addressed. Is the overall strategy the only one to be pursued? How can we move from current to future paradigms? Will we ever be able to reliably model for chronic and developmental neurotoxicity in vitro? This paper summarizes four presentations from a symposium held at the International Neurotoxicology Conference held in Xi"an, China, in June 2011. A. Li reviewed the current guidelines for neurotoxicity and developmental neurotoxicity testing, and discussed the major challenges existing to realize the NCR vision for toxicity testing. J. Llorens reviewed the biology of mammalian toxic avoidance in view of present knowledge on the physiology and molecular biology of the chemical senses, taste and smell. This background information supports the hypothesis that relating in vivo toxicity to chemical epitope descriptors that mimic the chemical encoding performed by the olfactory system may provide a way to the long term future of complete in silico toxicity prediction. S. Ceccatelli reviewed the implementation of rodent and human neural stem cells (NSCs) as models for in vitro toxicity testing that measures parameters such as cell proliferation, differentiation and migration. These appear to be sensitive endpoints that can identify substances with developmental neurotoxic potential. C. Sun ol reviewed the use of primary neuronal cultures in testing for neurotoxicity of environmental pollutants, including the study of the effects of persistent exposures and/or in differentiating cells, which allow recording of effects that can be extrapolated to human developmental neurotoxicity.

Alateraciones de la memoria implícita y explícita en el envejecimiento natural y en la demencia tipo Alzheimer

L’esperança de vida ha millorat en aquest darrer segle, al mateix temps, han augmentat les malalties neurodegeneratives, sent l’Alzheimer el seu màxim exponent, caracteritzant-se per una important pèrdua de memòria. Davant d’aquesta realitat, l’objectiu últim d’aquest treball, és corroborar que les alteracions de memòria (explícita i implícita) no formen part del procés d’envelliment natural. Per a tal propòsit, després d’assentar els aspectes generals sobre la memòria, ens centrarem amb l’anàlisi de les alteracions que es produeixen en la memòria explícita i implícita, tant durant el procés d’envelliment natural, com en el patològic, particularment en el cas de la malaltia d’Alzheimer. Per arribar a una conclusió fefaent, s'efectuarà un minuciós i crític estudi, revisant la literatura específica i científica existent.

Historia, patrimonio y territorio: políticas públicas de memoria en el frente del Segre y la frontera pirenaica catalana

La relación entre historia, patrimonio y territorio, en unos espacios culturales de clara relevancia durante la Guerra Civil y la Segunda Guerra Mundial, permite reflexionar sobre algunas políticas públicas de memoria desarrolladas a raíz de la confluencia que se ha dado entre el Programa Memorial Democràtic impulsado por la Generalitat de Catalunya, la acción decidida de algunos ayuntamientos comprometidos en preservar la memoria histórica, el mundo académico representado por la Universidad y el asociacionismo civil.

Use of a pre-sidedress soil nitrate test (PSNT) to determine nitrogen fertilizer requirements for irrigated corn

In the present study, a 2-year N rate response experiment was conducted in different fields to monitor NO3-N soil profiles, N accumulation by the crop and final crop performance, in order to assess if soil NO3-N at pre-sidedressing (Pre-Sidedress Soil Nitrate Test, PSNT) is a reliable indicator for soil N availability for corn in the irrigated area served by canal d’Urgell (Lleida, Spain), and if the test can be used to separate responsive fields from non-responsive fields to sidedress N fertilizer applications. Preliminary soil N availability (N sidedress fertilizer rate + PSNT) critical levels to identify fields that need supplementary N fertilizer applications were established at ca. 300 and 210 kg NO3-N·ha–1, for PSNTrooting–zone and PSNT0–30 cm, respectively (for a yield goal of 14 t grain·ha–1).

¿Cómo desarrollar la memoria en personas mayores desde la Teoría de las Inteligencias Múltiples de H. Gardner?

La falta de memoria suele ser una de las principales quejas de nuestros mayores. Tradicionalmente se han venido desarrollando distintos programas para mejorarla o mantenerla. En este artículo les presentamos una forma de abordar ese problema. Nuestro deseo es compartir nuestra experiencia. Les presentamos una unidad de programación desarrollada en el marco del Taller: Apuesta por tu Memoria que organiza el Ayuntamiento de Lleida, para los mayores de diferentes casales de la ciudad. La fundamentación teórica en la que enmarcamos nuestra intervención parte de la teoría de las inteligencias múltiples de Howard Gardner y del enfoque de las teorías del procesamiento de la información.

Memoria activa

En este artículo se presenta el programa Memoria Activa que se diseñó para el Área de Servicios Personales del Ayuntamiento de Lleida (España). Lleva aplicándose desde el año 2004 hasta la actualidad en los casales para personas mayores de titularidad municipal. Se explica el proceso de investigación seguido para la elaboración del programa. Una primea fase (diseño del programa, planificación y aplicación del programa y su respectiva evaluación) bajo la modalidad de investigación-acción cooperativa. Y una segunda fase bajo una metodología de investigación evaluativa. El modelo evaluativo que se propone en esta investigación es el modelo CIPP de Stufflebeam (2002). Se explican los objetivos, contenidos, metodología, recursos, evaluación del programa, a la vez que, se ejemplifican y desglosan algunas de las actividades.

Historia de las mujeres y memoria histórica: Manuela Sáenz interpela a Simón Bolívar (1822-1830)

En la construcción de la historia de las mujeres, es necesario revisar a fondo las manipulaciones de la memoria sobre la presencia femenina, ya sea oculta o visible, en todas las sociedades históricas y actuales. Las interpretaciones muy divergentes de un icono feme- nino, Manuela Sáenz – la más famosa amante de Simón Bolívar – son un ejemplo paradig- mático que nos permite reflexionar, desde lo particular, sobre temas de ámbito más general.

Nou web de la Biblioteca Virtual: test amb usuaris, desembre 2013

Informe de l'anàlisi realitzat sobre el nou web de la Biblioteca Virtual de la UOC, per tal d'avaluar el grau d'usabilitat de la nova eina. És el segon test amb usuaris que es realitza del nou web i s'han analitzat els següents aspectes: l'accés a la col·lecció digital, les cinc funcionalitats més utilitzades del web i les cinc funcionalitats menys visibles del web.

Problems of the randomization test for AB designs

N = 1 designs imply repeated registrations of the behaviour of the same experimental unit and the measurements obtained are often few due to time limitations, while they are also likely to be sequentially dependent. The analytical techniques needed to enhance statistical and clinical decision making have to deal with these problems. Different procedures for analysing data from single-case AB designs are discussed, presenting their main features and revising the results reported by previous studies. Randomization tests represent one of the statistical methods that seemed to perform well in terms of controlling false alarm rates. In the experimental part of the study a new simulation approach is used to test the performance of randomization tests and the results suggest that the technique is not always robust against the violation of the independence assumption. Moreover, sensitivity proved to be generally unacceptably low for series lengths equal to 30 and 40. Considering the evidence available, there does not seem to be an optimal technique for single-case data analysis

ISO standars on test methods for water radioactivity monitoring

Water is vital to humans and each of us needs at least 1.5 L of safe water a day to drink. Beginning as long ago as 1958 the World Health Organization (WHO) has published guidelines to help ensure water is safe to drink. Focused from the start on monitoring radionuclides in water, and continually cooperating with WHO, the International Standardization Organization (ISO) has been publishing standards on radioactivity test methods since 1978. As reliable, comparable and"fit for purpose" results are an essential requirement for any public health decision based on radioactivity measurements, international standards of tested and validated radionuclide test methods are an important tool for production of such measurements. This paper presents the ISO standards already published that could be used as normative references by testing laboratories in charge of radioactivity monitoring of drinking water as well as those currently under drafting and the prospect of standardized fast test methods in response to a nuclear accident.

From caging to rouse dynamics in polymer melts with intramolecular barriers: a critical test of the mode coupling theory

By means of computer simulations and solution of the equations of the mode coupling theory (MCT),we investigate the role of the intramolecular barriers on several dynamic aspects of nonentangled polymers. The investigated dynamic range extends from the caging regime characteristic of glass-formers to the relaxation of the chain Rouse modes. We review our recent work on this question,provide new results, and critically discuss the limitations of the theory. Solutions of the MCT for the structural relaxation reproduce qualitative trends of simulations for weak and moderate barriers. However, a progressive discrepancy is revealed as the limit of stiff chains is approached. This dis-agreement does not seem related with dynamic heterogeneities, which indeed are not enhanced by increasing barrier strength. It is not connected either with the breakdown of the convolution approximation for three-point static correlations, which retains its validity for stiff chains. These findings suggest the need of an improvement of the MCT equations for polymer melts. Concerning the relaxation of the chain degrees of freedom, MCT provides a microscopic basis for time scales from chain reorientation down to the caging regime. It rationalizes, from first principles, the observed deviations from the Rouse model on increasing the barrier strength. These include anomalous scaling of relaxation times, long-time plateaux, and nonmonotonous wavelength dependence of the mode correlators.

Establishment of an In Vitro Photoallergy Test Using NCTC2544 Cells and IL-18 Production

Differentiation between photoallergenic and phototoxic reactions induced by low molecular weight compounds represents a current problem. The use of eratinocytes as a potential tool for the detection of photoallergens as opposed to photoirritants is considered an interesting strategy for developing in vitro methods. We have previously demonstrated the possibility to use the human keratinocyte cell line NCTC2455 and the production of interleukin-18 (IL-18) to screen low molecular weight sensitizers. The purpose of this work was to explore the possibility to use the NCTC2544 assay to identify photoallergens and discriminate from phototoxic chemicals. First, we identified suitable condition of UV-irradiation (3.5 J/cm2) by investigating the effect of UVAirradiation on intracellular IL-18 on untreated or chloropromazine (a representative phototoxic compound)- treated NCTC2544 cells. Then, the effect of UVA-irradiation over NCTC2544 cells treated with increasing concentrations of 15 compounds including photoallergens (benzophenone, 4-ter-butyl-4-methoxydibenzoylmethane, 2-ethylexyl-p-methoxycinnamate, ketoprofen, 6-methylcumarin); photoirritant and photoallergen (4-aminobenzoic acid, chlorpromazine, promethazine); photoirritants (acridine, ibuprofen, 8-methoxypsoralen, retinoic acid); and negative compounds (lactic acid, SDS and p-phenilendiamine) was investigated. Twenty-four hours after exposure, cytotoxicity was evaluated by the MTT assay or LDH leakage, while ELISA was used to measure the production of IL-18. At the maximal concentration assayed with non-cytotoxic effects (CV80 under irradiated condition), all tested photoallergens induced a significant and a dose-dependent increase of intracellular IL-18 following UVA irratiation, whereas photoirritants failed. We suggest that this system may be useful for the in vitro evaluation of the photoallergic potential of chemicals.

2000-2011 El lugar de la memoria

La historia de España durante el siglo XX está marcada por una serie de episodios dramáticos, que producen un distanciamiento social muy complejo. El largo periodo franquista que sucede a una guerra fratricida de tres años no es condenado y revisado por las instituciones democráticas hasta el año 2004, cuando la demanda ciudadana apela a la necesidad de restituir la memoria a más de 30.000 desaparecidos. Se inicia un proceso político, cultural y social que marcará una"segunda transición", la de la memoria histórica, superando de este modo el olvido acordado tras la muerte del dictador. Las competencias del desarrollo legal que marcan esta segunda transición son abordadas a escala autonómica, produciendo un desequilibrio territorial atenazado por intereses políticos. En Catalunya, se crea una nueva institución pública dedicada al desarrollo de la memoria histórica: el Memorial Democràtic. En este artículo abordamos de forma especial el interés prestado por esta institución a los llamados espacios de memoria; cuya singularidad será señalizada y dignificada, entendiendo el territorio a escala global y local.

Hacia los 25 años de los saqueos de la ciudad de Rosario, Argentina. Educación y memoria

A punto de cumplirse los 25 años de los saqueos producidos en Rosario de la República Argentina, este artículo se centra en el debate historiográfico y antropológico acerca del uso de esa categoría para describir el impacto producido entre los supermercados más afectados de la ciudad. Se relaciona este caso con sucesos acontecidos en el país, y en especial se reproducen y analizan los dibujos que elaboraron niños y niñas de tercer grado de escuela primaria durante el primer día en que la escuela reabrió sus puertas. El ámbito educativo forma parte de este análisis sobre el significado del saqueo y sobre su uso para garantizar la alimentación infantil.