Sujetos responsables por los daños derivados del uso de un producto peligroso. Comentario a la STS, 1a, 21.11.2008 (MP:José Antonio Seijas Quintana), RJ 2009\144

La STS, 1a, 21.11.2008, objeto de este comentario, plantea a un tiempo la responsabilidad del consumidor, vendedor, fabricante y Administración pública por los daños materiales causados por la explosión de un producto altamente inflamable que había sido vendido a un particular para la desinfección de su vivienda.

When cholesterol is not cholesterol: a note on the enzymatic determination of its concentration in model systems containing vegetable extracts

Background: Experimental evidences demonstrate that vegetable derived extracts inhibit cholesterol absorption in the gastrointestinal tract. To further explore the mechanisms behind, we modeled duodenal contents with several vegetable extracts. Results: By employing a widely used cholesterol quantification method based on a cholesterol oxidase-peroxidase coupled reaction we analyzed the effects on cholesterol partition. Evidenced interferences were analyzed by studying specific and unspecific inhibitors of cholesterol oxidase-peroxidase coupled reaction. Cholesterol was also quantified by LC/MS. We found a significant interference of diverse (cocoa and tea-derived) extracts over this method. The interference was strongly dependent on model matrix: while as in phosphate buffered saline, the development of unspecific fluorescence was inhibitable by catalase (but not by heat denaturation), suggesting vegetable extract derived H2O2 production, in bile-containing model systems, this interference also comprised cholesterol-oxidase inhibition. Several strategies, such as cholesterol standard addition and use of suitable blanks containing vegetable extracts were tested. When those failed, the use of a mass-spectrometry based chromatographic assay allowed quantification of cholesterol in models of duodenal contents in the presence of vegetable extracts. Conclusions: We propose that the use of cholesterol-oxidase and/or peroxidase based systems for cholesterol analyses in foodstuffs should be accurately monitored, as important interferences in all the components of the enzymatic chain were evident. The use of adequate controls, standard addition and finally, chromatographic analyses solve these issues.

Heat shock response in yeast involves changes in both transcription rates and mRNA stabilities

We have analyzed the heat stress response in the yeast Saccharomyces cerevisiae by determining mRNA levels and transcription rates for the whole transcriptome after a shift from 25uC to 37uC. Using an established mathematical algorithm, theoretical mRNA decay rates have also been calculated from the experimental data. We have verified the mathematical predictions for selected genes by determining their mRNA decay rates at different times during heat stress response using the regulatable tetO promoter. This study indicates that the yeast response to heat shock is not only due to changes in transcription rates, but also to changes in the mRNA stabilities. mRNA stability is affected in 62% of the yeast genes and it is particularly important in shaping the mRNA profile of the genes belonging to the environmental stress response. In most cases, changes in transcription rates and mRNA stabilities are homodirectional for both parameters, although some interesting cases of antagonist behavior are found. The statistical analysis of gene targets and sequence motifs within the clusters of genes with similar behaviors shows that both transcriptional and post-transcriptional regulons apparently contribute to the general heat stress response by means of transcriptional factors and RNA binding proteins.

Acotaciones a la traducción de clásicos grecolatinos y modernos de José María Valverde

Este trabajo es un comentario a un diálogo-entrevista con José María Valverde (Valencia de Alcántara/Cáceres/1926-Barcelona, 1996), en su vertiente estrictamente traductora. La importancia del comentario estriba en el hecho de que muchas traducciones del eximio teórico de la literatura, poeta y traductor extremeño servirán, con el tiempo, de modelo de lengua y cultura. De ahí la importancia de leer en su contexto, reseñar y comentar sus opiniones. Así, pues en el trabajo se reproducen y se comentan hasta seis items: 1. Para traducir bien, hay que oír al autor original. 2. El traductor tiene que vivir de su trabajo. 3. En Italia se traduce bien. 4. Está por escribir una historia del sentido del humor. 5. Cuando no existe una tradición hay que inventarla. 6. Lo primero es hablar; después traducir.

El exilio leridano de los canónigos y diputados a Cortes liberales Francisco Martínez Marina y José Espiga Gadea

En el presente trabajo vamos a analizar la trayectoria política de dos canónigos, Francisco Martínez Marina y José Espiga y Gadea, partidarios del liberalismo en una época de cambios en la cual, cada uno a su manera, contribuyó a la construcción del Estado liberal constitucional español. Tras las Cortes de Cádiz, estos importantes pensadores fueron perseguidos por el absolutismo y compartieron estancia en la Catedral de Lérida, el seno de la cual abandonaron para participar en las Cortes del Trienio Liberal (1820­-1823)

Plant-derived phenolics inhibit the accrual of structurally characterised protein and lipid oxidative modifications

Epidemiological data suggest that plant-derived phenolics beneficial effects include an inhibition of LDL oxidation. After applying a screening method based on 2,4-dinitrophenyl hydrazine- protein carbonyl reaction to 21 different plant-derived phenolic acids, we selected the most antioxidant ones. Their effect was assessed in 5 different oxidation systems, as well as in other model proteins. Mass-spectrometry was then used, evidencing a heterogeneous effect on the accumulation of the structurally characterized protein carbonyl glutamic and aminoadipic semialdehydes as well as for malondialdehyde-lysine in LDL apoprotein. After TOF based lipidomics, we identified the most abundant differential lipids in Cu++-incubated LDL as 1-palmitoyllysophosphatidylcholine and 1-stearoyl-sn-glycero-3-phosphocholine. Most of selected phenolic compounds prevented the accumulation of those phospholipids and the cellular impairment induced by oxidized LDL. Finally, to validate these effects in vivo, we evaluated the effect of the intake of a phenolic-enriched extract in plasma protein and lipid modifications in a well-established model of atherosclerosis (diet-induced hypercholesterolemia in hamsters). This showed that a dietary supplement with a phenolic-enriched extract diminished plasma protein oxidative and lipid damage. Globally, these data show structural basis of antioxidant properties of plant-derived phenolic acids in protein oxidation that may be relevant for the health-promoting effects of its dietary intake. that a dietary supplement with a phenolic-enriched extract diminished plasma protein oxidative and lipid damage. Globally, these data show structural basis of antioxidant properties of plant-derived phenolic acids in protein oxidation that may be relevant for the health-promoting effects of its dietary intake.

Electron tunneling in heavily In-doped polycrystalline CdS films

The electrical properties of heavily In‐doped polycrystalline CdS films have been studied as a function of the doping level. The films were prepared by vacuum coevaporation of CdS and In. Conductivity and Hall measurements were performed over the temperature range 77-400 K. The conductivity decreases weakly with the temperature and shows a tendency towards saturation at low temperatures. A simple relationship σ=σ0(1+βT2) is found in the low‐temperature range. The temperature dependence of the mobility is similar to that of the conductivity since the Hall coefficient is found to be a constant in the whole temperature range. We interpret the experimental results in terms of a modified version of grain‐boundary trapping Seto"s model, taking into account thermionic emission and tunneling of carriers through the potential barriers. The barriers are found to be high and narrow, and tunneling becomes the predominating transport mechanism.

Multislice CT for assessing in-stent dimensions after left main coronary artery stenting: a comparison with three dimensional intravascular ultrasound

Objective: To evaluate the agreement between multislice CT (MSCT) and intravascular ultrasound (IVUS) to assess the in-stent lumen diameters and lumen areas of left main coronary artery (LMCA) stents. Design: Prospective, observational single centre study. Setting: A single tertiary referral centre. Patients: Consecutive patients with LMCA stenting excluding patients with atrial fibrillation and chronic renal failure. Interventions: MSCT and IVUS imaging at 9-12 months follow-up were performed for all patients. Main outcome measures: Agreement between MSCT and IVUS minimum luminal area (MLA) and minimum luminal diameter (MLD). A receiver operating characteristic (ROC) curve was plotted to find the MSCT cut-off point to diagnose binary restenosis equivalent to 6 mm2 by IVUS. Results: 52 patients were analysed. Passing-Bablok regression analysis obtained a β coefficient of 0.786 (0.586 to 1.071) for MLA and 1.250 (0.936 to 1.667) for MLD, ruling out proportional bias. The α coefficient was −3.588 (−8.686 to −0.178) for MLA and −1.713 (−3.583 to −0.257) for MLD, indicating an underestimation trend of MSCT. The ROC curve identified an MLA ≤4.7 mm2 as the best threshold to assess in-stent restenosis by MSCT. Conclusions: Agreement between MSCT and IVUS to assess in-stent MLA and MLD for LMCA stenting is good. An MLA of 4.7 mm2 by MSCT is the best threshold to assess binary restenosis. MSCT imaging can be considered in selected patients to assess LMCA in-stent restenosis

La presencia de la mujer en la poesía de José Agustín Goytisolo

El 19 de marzo del pasado año 2009 se cumplieron diez años de la muerte de José Agustín Goytisolo. Con este motivo se han sucedido los homenajes y congresos que se han ocupado de estudiar su poesía y de repasar su legado desde ángulos muy diversos. Este artículo aspira también a rellenar un hueco que –sorprendentemente o no– no ha merecido hasta el presente especial atención: la presencia de la mujer en la poesía goytisoliana. Mi objetivo es trazar una panorámica del lugar que las mujeres ocupan en sus versos y mostrar que en su gran mayoría son diferentes de las figuras femeninas que pueblan la tradición literaria. En primer lugar porque, desde el punto de vista de la enunciación, la voz poética no se apropia de la mujer convirtiéndola en objeto de contemplación, sino que, en general, es sujeto de la historia en paridad con el poeta. En segundo lugar, porque las mujeres goytisolianas tampoco se ajustan a los “códigos” a los que las ha relegado el discurso patriarcal: tienen vida propia y no funcionan como símbolo de aspiraciones ajenas ni como proyección del imaginario masculino.

La colección Marca Hispanica, un ambicioso proyecto de traducción de José Agustín Goytisolo

In May 1985 José Agustín Goytisolo launched an ambitious project to translate into Spanish works originally written in Catalan. To this end, with a small group of collaborators he founded the Marca Hispanica collection. Its aim was to spread Catalan literature beyond its borders to reach millions of readers of Spanish through schools, teachers and language academies around the world. The initial idea was to make available in bilingual editions a hundred representative works of Catalan literature but, in the event, only twenty-two volumes were ever published. Based on access to unpublished documents, in this article we defend this endeavour, report on its progress and relate the circumstances, beyond the control of the participants, that led to its demise.

Ilustración y nihilismo en los Artículos de Mariano José de Larra

En la primera parte de este artículo se muestra cómo el concepto de ser humano que Larra utiliza en sus escritos es de clara raigambre ilustrada. En la segunda, se analiza cómo el autor de los Artículos, tras tomar conciencia de los límites de dicho ideal, reaccionó con un extremismo nihilista que le llevó a ver como detestables aquellas capacidades humanas que los ilustrados habían valorado positivamente: el lenguaje, la racionalidad y la sociabilidad. En la tercera parte, se estudia, a la luz de los escritos de Friedrich Nietzsche, los dos tipos de nihilismo que se enfrentaron en el espíritu y en la obra de Larra.

On the Hall effect in polycrystalline semiconductors

Some problems involved in the interpretation of Hall‐effect measurements in polycrystalline semiconductors have not been resolved, especially when the contribution of the boundaries is appreciable. Using the Herring theory of transport properties in inhomogeneous semiconductors, we present an alternative interpretation to that previously proposed. This model permits the calculation of the Hall coefficient under general conditions.