Development of Chitosan and Poly (Vinyl Alcohol) Blended scaffolds for cell culture using supercritical fluids technology

Disserta����o apresentada na Faculdade de Ci��ncias e Tecnologia da Universidade Nova de Lisboa para obten����o do grau de Mestre em Mestrado Integrado em Engenharia Qu��mica e Bioqu��mica

Production of recombinant protein hLIF in static and dynamic systems of animal cell culture

Thesis for the Degree of Master of Science in Biotechnology Universidade Nova de Lisboa, Faculdade de Ci��ncias e Tecnologia

���We are azorean���: discourses and practices of folk culture in Santa Catarina (Brazil)

Etnogr��fica, Vol. IX, N.1, pp. 171-193

The impact of CdSe/ZnS Quantum Dots in cells of Medicago sativa in suspension culture

Abstract Background: Nanotechnology has the potential to provide agriculture with new tools that may be used in the rapid detection and molecular treatment of diseases and enhancement of plant ability to absorb nutrients, among others. Data on nanoparticle toxicity in plants is largely heterogeneous with a diversity of physicochemical parameters reported, which difficult generalizations. Here a cell biology approach was used to evaluate the impact of Quantum Dots (QDs) nanocrystals on plant cells, including their effect on cell growth, cell viability, oxidative stress and ROS accumulation, besides their cytomobility. Results: A plant cell suspension culture of Medicago sativa was settled for the assessment of the impact of the addition of mercaptopropanoic acid coated CdSe/ZnS QDs. Cell growth was significantly reduced when 100 mM of mercaptopropanoic acid -QDs was added during the exponential growth phase, with less than 50% of the cells viable 72 hours after mercaptopropanoic acid -QDs addition. They were up taken by Medicago sativa cells and accumulated in the cytoplasm and nucleus as revealed by optical thin confocal imaging. As part of the cellular response to internalization, Medicago sativa cells were found to increase the production of Reactive Oxygen Species (ROS) in a dose and time dependent manner. Using the fluorescent dye H2DCFDA it was observable that mercaptopropanoic acid-QDs concentrations between 5-180 nM led to a progressive and linear increase of ROS accumulation. Conclusions: Our results showed that the extent of mercaptopropanoic acid coated CdSe/ZnS QDs cytotoxicity in plant cells is dependent upon a number of factors including QDs properties, dose and the environmental conditions of administration and that, for Medicago sativa cells, a safe range of 1-5 nM should not be exceeded for biological applications.

��Tylorian Professors�� e ��Japanese Corporals��: teoria antropol��gica e identidade nacional na etnografia portuguesa

Ethnologia, V. 12-14, pp. 37-52

���Culture Stops Development!���: Bijag�� Youth and the Appropriation of Developmentalist Discourse in Guinea-B

African Studies Review, Volume 52, Number 2, pp. 69���

Ethno-pragmatique et prosodie. Pr��sentation d'une hypoth��se de recherche pluridisciplinaire dans le domaine des sciences du langage et de la culture

L'hypoth��se de recherche que nous souhaitons vous pr��senter ici a pour objet l&s fonctions pragmatiques de Ia prosodie. L'ensemble de notre Projet de Recherche s'organise en effet autour d'une seule (mais fondamentale) hypoth��se de travail: c'est dans et par Ia prosodie que pour Vessentiel le langage contribue activement �� Ia construction du lien social. La formulation de cette hypoth��se resulte des travaux que nous avons men��s dans le champ de FAnthropologie des rites, ��tudi��s dans une perspective s��miologique (au sens large), visant �� mieux saisir dans quelle mesure et selon quelles modalit��s, dans et par le rite, Vordre du signe est signe de 1'ordreK Le rite constitue un syst��me complexe de comrpunication symbolique qui contribue en effet activement �� definir sur le plan s��mantique et �� structurer sur le plan dramaturgique les situations d'interaction sociale.

Culture boundaries in semantic web

Dissertation submitted in partial fulfillment of the requirements for the Degree of Master of Science in Geospatial Technologies.

Organizational culture influence on information quality - use of business intelligence systems relationship: portuguese context

Disserta����o apresentada como requisito parcial para obten����o do grau de Mestre em Estat��stica e Gest��o de Informa����o

A systems biology framework for pathway level culture media engineering: pplication to Pichia pastoris cultures

Disserta����o para obten����o do Grau de Doutor em Engenharia Qu��mica e Bioqu��mica

The Visual Culture of Human Rights. Observations on Amnesty International

Trabalho de project de Mestrado em Antropologia de Direitos Humanos e Movimentos Sociais

Gas emissions on rice culture and its importance on air quality

Disserta����o para obten����o do Grau de Mestre em Engenharia do Ambiente Perfil Gest��o de Sistemas Ambientais

International News Reporting in the Multidimensional Network: The socio-demographics, professional culture and newswork of foreign correspondents working across Sub-Saharan Africa

Tese apresentada para cumprimento dos requisitos necess��rios �� obten����o do grau de Doutor em Media Digitais

Production of polyhydroxyalkanoates from cheese whey - pH effect on the acidogenic fermentation stage and nutrient needs of the culture selection stage

Disserta����o para obten����o do Grau de Mestre em Biotecnologia

Things we see: Portuguese anthropology on material culture

Part of the objects that anthropologists can now find in Lisbon result from the existence of networks with rather diverse historical, social and cultural origins, linking Angola, Mozambique, Cape Verde, Guinea-Bissau, Brazil and Portugal, as well as the countries which have attracted all these countries��� diasporas. The publishing of papers by Portuguese and Brazilian anthropologists in this dossier dedicated to consumption might come to generate a productive collaboration between researchers from both countries, which for over five centuries have seen arriving from the other side of the Atlantic strange objects that, in turn, have taken the routes of the diasporas mentioned above, from luxurious and whimsical items as the indigenous leaders��� feathers and the carriages of the Portuguese royalty, to common and irreplaceable goods as the havaianas.