Estudo descritivo da unidade de cuidados intensivos e intermédios respiratórios do Hospital Clínico - Barcelona

Este estudo tem como objectivo descrever a Unidade de cuidados intensivos e intermédios respiratórios do Hospital Clínico em Barcelona de forma a caracterizá-la tanto ao nível de recursos materiais e humanos como em termos de protocolos usados. Esta unidade é considerada uma das melhores da Europa, dispondo da mais alta tecnologia, desenvolvendo protocolos baseados na evidência de forma a reduzir ao máximo a taxa de mortalidade neste local. O staff médico sempre em constante actualização, trabalhando muito no campo da investigação. Por outro lado, a fisioterapia integrante no Instituto Clínico do Tórax realiza menos investigação investindo mais no tratamento de doentes não intubados, tendo como técnicas de eleição posicionamento, mobilização, expiração forçada e hidratação oral. Em relação à VNI, muitos dos estudos referentes a este tema foram realizados pelo staff médico deste Hospital. O staff de fisioterapia é responsável pela adaptação da VNI nos pacientes hipercápnicos. Em conclusão, o número e tempo limitado de fisioterapeutas na unidade não contribuem para a aplicação de novas técnicas que requerem a intervenção de dois fisioterapeutas. Palavras-chave: unidade de cuidados intensivos respiratórios, unidade de cuidados intermédios respiratórios, VNI, papel dos fisioterapeutas respiratórios.

O Tratamento dos activos fixos tangíveis no sistema de normalização contabilística proposto e a sua comparação com o POC

Jornadas de Contabilidade e Fiscalidade promovidas pelo Instituto Superior de Contabilidade e Administração do Porto, em Abril de 2009

Norma contabilística e de relato financeiro 10 – custos de empréstimos obtidos

Dissertação de Mestrado submetida ao Instituto Superior de Contabilidade e Administração do Porto para a obtenção do grau de Mestre em Auditoria Trabalho efetuado sob a orientação do Mestre Carlos Martins

Avaliação da função muscular do ombro, em jogadores de voleibol, com e sem dor

Introdução: A sequência de activação e a actividade muscular, são importantes na coordenação do movimento da omoplata com a elevação do úmero. Objectivos: Avaliar sequência de activação, actividade e força de músculos do ombro comparando jogadores com/sem dor. Metodologia: Amostra de 15 atletas (7 - grupo experimental; 8 - grupo controlo). Avaliou-se electromiograficamente o Deltóide Anterior, Grande Peitoral, Trapézio Superior, Trapézio Inferior e Infra- Espinhoso na diagonal do Isocinético (90°/s; 180°/s). Resultados: Encontraram-se diferenças na sequência e tempos de activação; não havendo diferenças na actividade, peak torque e rácio antagonistas/agonistas. Conclusão: A dor crónica afectou sequência e tempos de activação muscular.

Learning objects recommendation in an adaptive educational hypermedia system

The aim of this paper is presenting the modules of the Adaptive Educational Hypermedia System PCMAT, responsible for the recommendation of learning objects. PCMAT is an online collaborative learning platform with a constructivist approach, which assesses the user’s knowledge and presents contents and activities adapted to the characteristics and learning style of students of mathematics in basic schools. The recommendation module and search and retrieval module choose the most adequate learning object, based on the user's characteristics and performance, and in this way contribute to the system’s adaptability.

PCMAT, an adaptive learning platform

This paper is about PCMAT, an adaptive learning platform for Mathematics in Basic Education schools. Based on a constructivist approach, PCMAT aims at verifying how techniques from adaptive hypermedia systems can improve e-learning based systems. To achieve this goal, PCMAT includes a Pedagogical Model that contains a set of adaptation rules that influence the student-platform interaction. PCMAT was subject to a preliminary testing with students aged between 12 and 14 years old on the subject of direct proportionality. The results from this preliminary test are quite promising as they seem to demonstrate the validity of our proposal.

PCMAT Metadata Authoring Tool

This paper introduces the PCMAT platform project and, in particular, one of its components, the PCMAT Metadata Authoring Tool. This is an educational web application that allows the project metadata creators to write the metadata associated to each learning object without any concern for the metadata schema semantics. Furthermore it permits the project managers to add or delete elements to the schema, without having to rewrite or compile any code.

Adaptation model for PCMAT – Mathematics collaborative learning platform

The aim of this paper is to present an adaptation model for an Adaptive Educational Hypermedia System, PCMAT. The adaptation of the application is based on progressive self-assessment (exercises, tasks, and so on) and applies the constructivist learning theory and the learning styles theory. Our objective is the creation of a better, more adequate adaptation model that takes into account the complexities of different users.

Content adaptation for an adaptive hypermedia system

The aim of this paper is presenting the recommendation module of the Mathematics Collaborative Learning Platform (PCMAT). PCMAT is an Adaptive Educational Hypermedia System (AEHS), with a constructivist approach, which presents contents and activities adapted to the characteristics and learning style of students of mathematics in basic schools. The recommendation module is responsible for choosing different learning resources for the platform, based on the user's characteristics and performance. Since the main purpose of an adaptive system is to provide the user with content and interface adaptation, the recommendation module is integral to PCMAT’s adaptation model.

Apanha-me se puderes! tradução comentada do capítulo “White-collar and Corporate Crime”, The Oxford Handbook of Criminology

Dissertação de Mestrado Apresentada ao Instituto Superior de Contabilidade e Administração do Porto para a obtenção do grau de Mestre em Tradução e Interpretação Especializadas, sob orientação da Mestre Suzana Noronha Cunha

Celiac disease detection using a transglutaminase electrochemical immunosensor fabricated on nanohybrid screen-printed carbon electrodes

Celiac disease is a gluten-induced autoimmune enteropathy characterized by the presence of tissue tranglutaminase (tTG) autoantibodies. A disposable electrochemical immunosensor (EI) for the detection of IgA and IgG type anti-tTG autoantibodies in real patient’s samples is presented. Screen-printed carbon electrodes (SPCE) nanostructurized with carbon nanotubes and gold nanoparticles were used as the transducer surface. This transducer exhibits the excellent characteristics of carbon–metal nanoparticle hybrid conjugation and led to the amplification of the immunological interaction. The immunosensing strategy consisted of the immobilization of tTG on the nanostructured electrode surface followed by the electrochemical detection of the autoantibodies present in the samples using an alkaline phosphatase (AP) labelled anti-human IgA or IgG antibody. The analytical signal was based on the anodic redissolution of enzymatically generated silver by cyclic voltammetry. The results obtained were corroborated with a commercial ELISA kit indicating that the electrochemical immunosensor is a trustful analytical screening tool.

Intra- and interspecific mineral composition variability of commercial instant coffees and coffee substitutes: contribution to mineral intake

The present paper reports the amount and estimated daily mineral intake of nine elements (Ca, Mg, K, Na, P, Fe, Mn, Cr and Ni) in commercial instant coffees and coffee substitutes (n = 49). Elements were quantified by high-resolution continuum source flame (HR-CS-FAAS) and graphite furnace (HR-CS-GFAAS) atomic absorption spectrometry, while phosphorous was evaluated by a standard vanadomolybdophosphoric acid colorimetric method. Instant coffees and coffee substitutes are rich in K, Mg and P (>100 mg/100 g dw), contain Na, Ca and Fe in moderate amounts (>1 mg/100 g), and trace levels of Cr and Ni. Among the samples analysed, plain instant coffees are richer in minerals (p < 0.001), except for Na and Cr. Blends of coffee substitutes (barley, malt, chicory and rye) with coffee (20–66%) present intermediate amounts, while lower quantities are found in substitutes without coffee, particularly in barley. From a nutritional point of view the results indicate that the mean ingestion of two instant beverages per day (total of 4 g instant powder), either with or without coffee, cannot be regarded as important sources of minerals to the human diet, although providing a supplementation of some minerals, particularly Mg and Mn in instant coffees. Additionally, and for authentication purposes, the correlations observed between some elements and the coffee percentage in the blends, with particular significance for Mg amounts, provides a potential tool for the estimation of coffee in substitute blends.

Voltammetric immunosensor for the diagnosis of celiac disease based on the quantification of anti-gliadin antibodies

Antibodies against gliadin are used to detect celiac disease (CD) in patients. An electrochemical immunosensor for the voltammetric detection of human anti-gliadin antibodies (AGA) IgA and AGA IgG in real serum samples is proposed. The transducer surface consists of screen-printed carbon electrodes modified with a carbon nanotube/gold nanoparticle hybrid system, which provides a very useful surface for the amplification of the immunological interactions. The immunosensing strategy is based on the immobilization of gliadin, the antigen for the autoantibodies of interest, onto the nanostructured surface. The antigen–antibody interaction is recorded using alkaline phosphatase labeled anti-human antibodies and a mixture of 3-indoxyl phosphate with silver ions (3-IP/Ag+) was used as the substrate. The analytical signal is based on the anodic redissolution of the enzymatically generated silver by cyclic voltammetry. The electrochemical behavior of this immunosensor was carefully evaluated assessing aspects as sensitivity, non-specific binding and matrix effects, and repeatability and reproducibility. The results were supported with a commercial ELISA test.

Development of polyaniline microarray electrodes for cadmium analysis

Disposable screen-printed electrodes (SPCE) were modified using a cosmetic product to partially block the electrode surface in order to obtain a microelectrode array. The microarrays formed were electropolymerized with aniline. Scanning electron microscopy was used to evaluate the modified and polymerized electrode surface. Electrochemical characteristics of the constructed sensor for cadmium analysis were evaluated by cyclic and square-wave voltammetry. Optimized stripping procedure in which the preconcentration of cadmium was achieved by depositing at –1.20 V (vs. Ag/AgCl) resulted in a well defined anodic peak at approximately –0.7 V at pH 4.6. The achieved limit of detection was 4 × 10−9 mol dm−3. Spray modified and polymerized microarray electrodes were successfully applied to quantify cadmium in fish sample digests.

Metal accumulation and oxidative stress biomarkers in octopus (Octopus vulgaris) from Northwest Atlantic

Metals are ubiquitous in the environment and accumulate in aquatic organisms and are known for their ability to enhance the production of reactive oxygen species (ROS). In aquatic species, oxidative stress mechanisms have been studied by measuring antioxidant enzyme activities and oxidative damages in tissues. The aim of this study was to apply and validate a set of oxidative stress biomarkers and correlate responses with metal contents in tissues of common octopus (Octopus vulgaris). Antioxidant enzyme activity (catalase — CAT, superoxide dismutase — SOD and glutathione S-transferases — GST), oxidative damages (lipid peroxidation — LPO and protein carbonyl content — PCO) andmetal content (Cu, Zn, Pb, Cd and As) in the digestive gland and armof octopus, collected in the NWPortuguese coast in different periods, were assessed after capture and after 14 days in captivity. CAT and SOD activitieswere highly responsive to fluctuations inmetal concentrations and able to reduce oxidative damage, LPO and PCO in the digestive gland. CAT activity was also positively correlated with SOD and GST activities, which emphasizes that the three enzymes respond in a coordinated way to metal induced oxidative stress. Our results validate the use of oxidative stress biomarkers to assess metal pollution effects in this ecological and commercial relevant species.Moreover, octopus seems to have the ability to control oxidative damage by triggering an antioxidant enzyme coordinated response in the digestive gland.