Semipermeable capsules wrapping a multifunctional and self-regulated co-culture microenvironment for osteogenic differentiation

A new concept of semipermeable reservoirs containing co-cultures of cells and supporting microparticles is presented, inspired by the multi-phenotypic cellular environment of bone. Based on the deconstruction of the â stem cell nicheâ , the developed capsules are designed to drive a self-regulated osteogenesis. PLLA microparticles functionalized with collagen I, and a co-culture of adipose stem (ASCs) and endothelial (ECs) cells are immobilized in spherical liquified capsules. The capsules are coated with multilayers of poly(L-lysine), alginate, and chitosan nano-assembled through layer-by-layer. Capsules encapsulating ASCs alone or in a co-culture with ECs are cultured in endothelial medium with or without osteogenic differentiation factors. Results show that osteogenesis is enhanced by the co-encapsulation, which occurs even in the absence of differentiation factors. These findings are supported by an increased ALP activity and matrix mineralization, osteopontin detection, and the up regulation of BMP-2, RUNX2 and BSP. The liquified co-capsules also act as a VEGF and BMP-2 cytokines release system. The proposed liquified capsules might be a valuable injectable self-regulated system for bone regeneration employing highly translational cell sources.

Teatro inserido nas unidades curriculares como forma de ajuda no desenvolvimento de crianças e jovens

Dissertação de mestrado em Comunicação, Arte e Cultura

Influence of different fibers on the change of pore pressure of self-consolidating concrete exposed to fire

The focus of this paper is given to investigate the effect of different fibers on the pore pressure of fiber reinforced self-consolidating concrete under fire. The investigation on the pore pressure-time and temperature relationships at different depths of fiber reinforced self-consolidating concrete beams was carried out. The results indicated that micro PP fiber is more effective in mitigating the pore pressure than macro PP fiber and steel fiber. The composed use of steel fiber, micro PP fiber and macro PP fiber showed clear positive hybrid effect on the pore pressure reduction near the beam bottom subjected to fire. Compared to the effect of macro PP fiber with high dosages, the effect of micro PP fiber with low fiber contents on the pore pressure reduction is much stronger. The significant factor for reduction of pore pressure depends mainly on the number of PP fibers and not only on the fiber content. An empirical formula was proposed to predict the relative maximum pore pressure of fiber reinforced self-consolidating concrete exposed to fire by considering the moisture content, compressive strength and various fibers. The suggested model corresponds well with the experimental results of other research and tends to prove that the micro PP fiber can be the vital component for reduction in pore pressure, temperature as well spalling of concrete.

A cooperar é que se aprende?: a aprendizagem cooperativa e educação intercultural: um contributo na construção da autonomia do aluno

Relatório de estágio de mestrado em Ensino de Inglês e Espanhol no 3º Ciclo do Ensino Básico e Ensino Secundário

Self-esteem, assertiveness and resilience in adolescents institutionalized

The institutionalization of children and adolescents has been an increasingly visible problem in modern society. Unfavourable socio-economic conditions have been joining the behavior problems and school absenteeism. When the family fails in its competence for education, social security or the Court withdraws the child or adolescent to a host institution. The aim of this research was to characterize self-esteem, assertiveness and resilience of institutionalized adolescents in the northern region of Portugal and to establish associations with these dependent variables and gender, scholar level and duration of the institutionalization. For the purpose of this study a wider questionnaire was carried out, and validated with a smaller group. It was a transversal study following a predominantly quantitative methodology, with a convenience sample. The sample included 101 adolescents (55 female and 46 males) from eight institutions, aged between 11 to 21 years old (average 15.45). For self-esteem the Rosenberg Self-Esteem Scale (Rosenberg, 1965), already validate for Portuguese adolescents, was used. For assertiveness and resilience it was applied the Global Evaluation Scale of Assertiveness and the Global Evaluation Scale of Resilience (Jardim & Pereira, 2006) we previously adapted and validated for adolescents. Collected data was introduced in a SPSS database. A descriptive analysis was done to characterize the sample concerning all the variables. To establish associations between individual factors and dependent variables t test, correlations and non-parametric test were applied. Results indicated a relatively low self-esteem (28.03), with girls having a lower value than boys, without significant differences. No correlations were found between self-esteem and the time in the institution. Assertiveness of the sample is average (23.97) and higher for girls than boys, with a positive significant correlation with the scholar grade. Also the resilience is average (25.97), having girls a little lower mean than boys and no significant differences or correlations were found.

Os sentidos da educação religiosa na construção do eu contemporâneo: espiritualidades juvenis

Dissertação de mestrado em Ciências da Educação (área de especialização em Sociologia da Educação e Políticas Educativas)

Self-reported psychosocial factors among In Vitro Fertilization patients interviewed alone or with the partner

"Published online: 07 Nov 2015"