Turning the Kaleidoscope - (E)FL Educational Experience and Inquiry as Auto/biography

This book is a study on learning, teaching/counselling, and research on the two. My quest has been to find a pedagogically-motivated way of researching learning and teaching interaction, and in particular counselling, in an autonomous language-learning environment. I have tried to develop a method that would make room for lived experience, meaning-making and narrating, because in my view these all characterise learning encounters between language learners and counsellors, and learners and their peers. Lived experience as a source of meaning, telling and co-telling becomes especially significant when we try to listen to the diverse personal and academic voices of the past as expressed in autobiographical narratives. I have aimed at researching various ALMS dialogues (Autonomous Learning Modules, University of Helsinki Language Centre English course and programme), and autobiographical narratives within them, in a way that shows respect for the participants, and that is relevant, reflective and, most importantly, self-reflexive. My interest has been in autobiographical telling in (E)FL [(English as a) foreign language], both in students first-person written texts on their language- learning histories and in the sharing of stories between learners and a counsellor. I have turned to narrative inquiry in my quest and have written the thesis as an experiential narrative. In particular, I have studied learners and counsellors in one and the same story, as characters in one narrative, in an attempt to avoid the impression that I am telling yet another separate, anecdotal story, retrospectively. Through narrative, I have shed light on the subjective dimensions of language learning and experience, and have come closer to understanding the emotional aspects of learning encounters. I have questioned and rejected a distanced and objective approach to describing learning and teaching/counselling. I have argued for a holistic and experiential approach to (E)FL encounters in which there is a need to see emotion and cognition as intertwined, and thus to appreciate learners and counsellors emotionally-charged experiences as integral to their identities. I have also argued for a way of describing such encounters as they are situated in history, time, autobiography, and the learning context. I have turned my gaze on various constellations of lived experience: the data was collected on various occasions and in various settings during one course and consists of videotaped group sessions, individual counselling sessions between students and their group counsellor, biographic narrative interviews with myself, open-ended personally-inspired reflection texts written by the students about their language-learning histories, and student logs and diaries. I do not consider data collection an unproblematic occasion, or innocent practice, and I defend the integrity of the research process. Research writing cannot be separated from narrative field work and analysing and interpreting the data. The foci in my work have turned to be the following: 1) describing ALMS encounters and specifying their narrative aspects; 2) reconceptualising learner and teacher autonomy in ALMS and in (E)FL; 2) developing (E)FL methodologically through a teacher-researcher s identity work; 4) research writing as a dialogical narrative process, and the thesis as an experiential narrative. Identity and writing as inquiry, and the deeply narrative and autobiographical nature of the (E)FL teaching/counselling/researching have come to the fore in this research. Research writing as a relational activity and its implications for situated ways of knowing and knowledge turned out to be important foci. I have also focussed on the context-bound and local teacher knowledge and ways of knowing about being a teacher, and I have argued for personal ways of knowing about, and learning and studying foreign languages. I discuss research as auto/biography: as a practising counsellor I use my own life and (E)FL experience to understand and interpret the stories of the research participants even though I was not involved in their course work. The supposedly static binaries of learner/teacher, and also learner autonomy/teacher autonomy, are thus brought into the discussion. I have highlighted the infinite variability and ever-changing nature of learning and teaching English, but the book is also of relevance to foreign language education in general.

Sään ääri-ilmiöt luonnollista vaihtelua kuvaavassa 1200-vuotisessa ilmastokokeessa

Tämän tutkielman tavoitteena oli selvittää erimittaisten sään ääri-ilmiöiden jaksojen (lämpötila ja sademäärä) ja sään ääri-ilmiöiden yhdistelmien esiintymistä sekä todennäköisyyttä Suomessa nykyisessä ilmastossa havaintojen (1950-2009) ja ilmaston sisäistä luonnollista vaihtelua kuvaavan ilmastomallikokeen, ns. Millennium0001-kontrolliajon (1200 vuotta) tulosten pohjalta. Tutkielmassa tarkastellaan sään ääri-ilmiöiden yhdistelmien esiintyvyyttä myös kirjallisuuden ja synoptisten tarkastelujen avulla sekä arvioidaan niiden esiintymisten mahdollisia muutoksia ilmaston lämmetessä. Aluksi tutkittiin, miten malliaineisto ja havainnot poikkeavat toisistaan tilastollisesti lämpötilojen ja sademäärien osalta, minkä jälkeen kontrolliajon tuottamia suureita korjattiin havaittua keskimääräistä ilmastoa vastaaviksi. Keskeiset tulokset johdettiin erimittaisille äärimmäisen kylmille tai kuumille jaksoille ja äärimmäisen sateisille jaksoille. Tutkitut kuukaudet olivat tammi- ja heinäkuu. Esimerkiksi vuorokauden keskilämpötila 25,5 °C ylittyy heinäkuussa keskimäärin suuren osan Etelä- ja Keski-Suomea peittävän hilaruudun (300 km x 300 km) alueella 0,2 %:n todennäköisyydellä, sademäärä 29,7 mm samoin 0,2 %:n todennäköisyydellä. Tammikuussa samassa hilapisteessä seitsemän vuorokauden kylmin jakso -30,2 °C ja sateisin jakso 50,1 mm toistuu kerran n. 500 vuodessa. Kuukauden keskilämpötiloille ja sademäärille tehtiin korrelaatioanalyysejä, joiden perusteella saatiin selville, että Suomessa vähäsateinen loppukevät (huhti-kesäkuu) tai pelkkä kesäkuu edesauttavat keskimääräistä lämpimämmän heinäkuun esiintymistä. Selityksenä voidaan pitää maaperän pientä kosteussisältöä. Korrelaatiot kesäkuun sademäärän ja heinäkuun keski- lämpötilojen välillä vaihtelivat -0,26 ja -0,36 välillä tilastollisen merkitsevyystason ollessa yli 99,9 %. Ilmastonmuutos muuttaa sään ääri-ilmiöiden esiintyvyyttä ja voi mahdollisesti lisätä sään ääri-ilmiöiden yhdistelmien todennäköisyyttä tai yhteiskunnan ja luonnon haavoittuvuutta ääri-ilmiöille tai niiden yhdistelmille. Ääri-ilmiöt voivat aiheuttaa mittavia vahinkoja niin yhteiskunnalle kuin luonnollekin, joten tällaisiin tapahtumiin olisi tärkeää voida varautua etukäteen mahdollisten tuhojen minimoimiseksi. Olisikin tärkeää lisätä tutkimusta tästä aiheesta.

Pienet ketut Herran viinitarhassa : Katarismi Lauragaisin kylissä 1200-1245

Tutkimus käsittelee keskiajan suurinta harhaoppia katarismia Toulousin hiippakunnassa 1200-luvun ensimmäisellä puoliskolla. Tarkempi tutkimusalue on Lauragaisin maaseutu Toulousin ja Carcassonnen välissä. Alue oli Etelä-Ranskan katarismin sydänaluetta. Tutkimus käy läpi katarismin historian vuosina 1200 1245. Tuolloin se muuttui julkisesti siedetystä uskosta salassa harjoitettavaksi harhaopiksi. Erityisessä tarkkailussa ovat Lauragaisin kylät ja niiden asukkaiden suhde katarismiin. Tutkimus selvittää miten kiellettyihin kataarien tapaamisiin osallistuttiin Lauragaisissa ja kuinka suuri osa alueen väestöstä uskoi katarismiin. Tutkimus pohjautuu pitkälti Bernard de Caux n vuosien 1245 1246 inkvisition kuulustelujen rekisteriin, joka sisältää 5 471 lauragaisilaisen inkvisitiotunnustusen. Tarkempaan tarkasteluun on valittu seitsemän kylää ja niiden 1 069 inkvisitiotunnustusta. Niiden sisältämät tiedot on syötetty tutkimuksen apuna toimivaan relaatiotietokantaan. Kylien asukkaiden kuulusteluja on verrattu keskenään tietokannan avulla. Kuulustelujen perusteella on rekonstruoitu kataarien tapaamisia vuosina 1195 1245. Lisäksi tutkitaan tarkemmin katarismin toiminnan kannalta tärkeitä mekanismeja Saint-Martin-Lalanden kylässä. Tutkimuksen mukaan katarismi vetosi kaikkiin yhteiskuntaluokkiin kuuluneisiin ihmisiin. Sukusiteillä ja Lauragaisin kylien asukkaiden keskinäisellä solidaarisuudella oli suuri merkitys katarismin säilymisessä, kun harhaoppi yritettiin tukahduttaa. Inkvisitiot onnistuivat murtamaan kylien sisäisen solidaarisuuden 1240-luvulla. Tutkimuksen keskeisin tulos on arvion esittäminen katarismiin uskoneiden maallikoiden lukumäärästä. Noin kymmenesosa Lauragaisin aikuisväestöstä oli aktiivisia kataariuskovia. Jopa kaksi viidesosaa alueen asukkaista oli jossain elämänsä vaiheessa osallistunut kataarien kiellettyihin uskonnollisiin tilaisuuksiin. Katarismiin uskoneiden ja siihen kosketuksissa olleiden ihmisten määrä on keskeinen tieto, kun arvioidaan katolisen kirkon harhaopin vastaisia toimia. Katarismin tuhoamiseksi julistettiin 20 vuotta kestänyt ristiretki. Inkvisitiolta kului sen jälkeen vielä 90 vuotta katarismin tuhoamiseen. Katarismi oli merkittävä harhaoppi, koska se vetosi kiellettynäkin huomattavaan osaan Lauragaisin väestöstä.

Death in the Bolivian High Plateau : Burials and Tiwanaku Society

My Ph.D. dissertation presents a multi-disciplinary analysis of the mortuary practices of the Tiwanaku culture of the Bolivian high plateau, situated at an altitude of c. 3800 m above sea level. The Tiwanaku State (c. AD 500-1150) was one of the most important pre-Inca civilisations of the South Central Andes. The book begins with a brief introductory chapter. In chapter 2 I discuss methodological and theoretical developments in archaeological mortuary studies from the late 1960s until the turn of the millennium. I am especially interested in the issue how archaeological burial data can be used to draw inferences on the social structure of prehistoric societies. Chapter 3 deals with the early historic sources written in the 16th and 17th centuries, following the Spanish Conquest of the Incas. In particular, I review information on how the Incas manifested status differences between and within social classes and what kinds of burial treatments they applied. In chapter 4 I compare the Inca case with 20th century ethnographic data on the Aymara Indians of the Bolivian high plateau. Even if Christianity has affected virtually every level of Aymara religion, surprisingly many traditional features can still be observed in present day Aymara mortuary ceremonies. The archaeological part of my book begins with chapter 5, which is an introduction into Tiwanaku archaeology. In the next chapter, I present an overview of previously reported Tiwanaku cemeteries and burials. Chapter 7 deals with my own excavations at the Late Tiwanaku/early post-Tiwanaku cemetery site of Tiraska, located on the south-eastern shore of Lake Titicaca. During the 1998, 2002, and 2003 field seasons, a total of 32 burials were investigated at Tiraska. The great majority of these were subterranean stone-lined tombs, each containing the skeletal remains of 1 individual and 1-2 ceramic vessels. Nine burials have been radiocarbon dated, the dates in question indicating that the cemetery was in use from the 10th until the 13th century AD. In chapter 8 I point out that considerable regional and/or ethnic differences can be noted between studied Tiwanaku cemetery sites. Because of the mentioned differences, and a general lack of securely dated burial contexts, I feel that at present we can do no better than to classify most studied Tiwanaku burials into three broad categories: (1) elite and/or priests, (2) "commoners", and (3) sacrificial victims and/or slaves and/or prisoners of war. On the basis of such indicators as monumental architecture and occupational specialisation we would expect to find considerable status-related differences in tomb size, grave goods, etc. among the Tiwanaku. Interestingly, however, such variation is rather modest, and the Tiwanaku seem to have been a lot less interested in expending considerable labour and resources in burial facilities than their pre-Columbian contemporaries of many parts of the Central Andes.

Complicated Presence : The Unity of Being in Parmenides and Heidegger

The attempt to refer meaningful reality as a whole to a unifying ultimate principle - the quest for the unity of Being - was one of the basic tendencies of Western philosophy from its beginnings in ancient Greece up to Hegel's absolute idealism. However, the different trends of contemporary philosophy tend to regard such a speculative metaphysical quest for unity as obsolete. This study addresses this contemporary situation on the basis of the work of Martin Heidegger (1889-1976). Its methodological framework is Heidegger's phenomenological and hermeneutical approach to the history of philosophy. It seeks to understand, in terms of the metaphysical quest for unity, Heidegger's contrast between the first (Greek) beginning or "onset" (Anfang) of philosophy and another onset of thinking. This other onset is a possibility inherent in the contemporary situation in which, according to Heidegger, the metaphysical tradition has developed to its utmost limits and thereby come to an end. Part I is a detailed interpretation of the surviving fragments of the Poem of Parmenides of Elea (fl. c. 500 BC), an outstanding representative of the first philosophical beginning in Heidegger's sense. It is argued that the Poem is not a simple denial of apparent plurality and difference ("mortal acceptances," doxai) in favor of an extreme monism. Parmenides' point is rather to show in what sense the different instances of Being can be reduced to an absolute level of truth or evidence (aletheia), which is the unity of Being as such (to eon). What in prephilosophical human experience is accepted as being is referred to the source of its acceptability: intelligibility as such, the simple and undifferentiated presence to thinking that ultimately excludes unpresence and otherness. Part II interprets selected key texts from different stages in Heidegger's thinking in terms of the unity of Being. It argues that one aspect of Heidegger's sustained and gradually deepening philosophical quest was to think the unity of Being as singularity, as the instantaneous, context-specific, and differential unity of a temporally meaningful situation. In Being and Time (1927) Heidegger articulates the temporal situatedness of the human awareness of meaningful presence. His later work moves on to study the situational correlation between presence and the human awareness. Heidegger's "postmetaphysical" articulation seeks to show how presence becomes meaningful precisely as situated, in an event of differentiation from a multidimensional context of unpresence. In resigning itself to this irreducibly complicated and singular character of meaningful presence, philosophy also faces its own historically situated finitude. This resignation is an essential feature of Heidegger's "other onset" of thinking.

En sjukdom af högst elakt släckte : Återfallsfebern på Sveaborg och i Karlskrona 1788-1790

Sommaren 1788 drabbades den svenska flottan av en svårartad febersjukdom. Febern, som senare definierats som febris recurrens el. återfallsfeber, hade sitt ursprung i den ryska flottan. Besättningen ombord skeppet Vladislav, krigsbytet från slaget vid Hogland, bar på ett stort antal smittade klädlöss. Efter flottans ankomst till Sveaborg spred sig sjukdomen snabbt bland manskapet, men även bland fästningens garnison. Förhållandena inom militären, både inom lantarmén och framför allt inom flottan, var gynnsamma för epidemiers spridning. De trånga utrymmena, den ensidiga kosten, det undermåliga dricksvattnet, den bristande hygienen: allt gynnade uppkomsten och spridningen av olika epidemier. Manskapets försämrade allmäntillstånd gjorde, att sjukdomarna blev mera förödande än vad de i andra förhållanden skulle ha varit. Bristen på manskap och material under Gustav III:s ryska krig var enormt, bl.a. var bristen på medicinsk personal och -utrustning skriande. Då flottan och armén drabbades av en epidemi av katastrofala dimensioner stod myndigheterna hjälplösa. Epidemin visaqr tydligt hur illa förberett hela kriget var och hur misskött flottans sjukvård var. På Sveaborg var förhållandena fruktansvärda. Halva garnisonen uppges ha avlidit, och det låg travar av lik överallt. Kaserner m.fl. byggnader adapterades till provisoriska lasarett och det rådde brist på allt. De medicinska myndigheterna representerades av den till fästningen skickade andra fältläkaren, som tillsammans med läkarna på fästningen gjorde sitt bästa i enlighet med tidens vårdmetoder. Då den svenska örlogsflottan i november seglat över tilll Karlskrona spred sig epidemin i staden. Sjukdomen grasserade också bland de civila. Då sjukdomens orsak och utbredningssätt var okända, kunde man varken hindra epidemin från att spridas eller genomföra adekvata vårdmetoder. Tvärtom, med de hemförlovade båtsmännen spred sig sjukdomen även till de övriga delarna av riket. Under 1789 var flottan p.g.a. de många sjukdomsfallen närmast operationsoduglig. Under vårvintern och våren 1790 avtog epidemin. Epidemin var ett svårt medicinskt problem. För att utreda situationen i Karlskrona skickade den tillförordnade regeringen, utredningskommissionen och Collegium medicum sina egna representanter till staden. De olika läkarnas sjukdomssyner grundade sig främst på tron om sjukdomars uppkomst genom miasma och förbättrandet av luftkvaliteten sågs som en väsentlig vårdform. I arbetet jämförs de olika myndigheternas och några av de på platsen varande läkarnas syn på sjukdomens art, dess orsaker och ursprung. De flesta härleder sjukdomen till den ryska flottan, och nämner någon form av smitta. Som främsta sjukdomsorsak nämns dock miasma och de rekommenderade vårdformerna representerade den humoralpatologiska synen. Förste amiralitetsläkaren Arvid Faxe representerar dock en annan åsikt, i det att han enbart tror på sjukdomens överföring via smitta. Epidemin var också ett politiskt problem. Epidemin var en lokal angelägenhet ända till dess att flottans operationer hämmades av manskapsbristen, varefter den blev ett ärende på högsta nivå. Kungen ingrep sommaren 1789 genom att grunda en kommision med rätt vidsträckta befogenheter. I Karlskrona verkar de militära myndigheterna och läkarna ha misstrott och skuldsatt varandra för katastrofen, och förhållandet mellan de till staden sända utredarna och militärerna var likaså inflammerat. Genom källorna återspeglas rivalitet, avund och inbördes konkurrens. Personalbristen var svår, och den skyldiga söktes utanför den egna kretsen. Den danskfödde apotekaren med sina påstott otjänliga mediciner blev en ypperlig syndabock. Örlogsflottan beräknas i sjukdomar ha förlorat omkring 10.000 man i döda, huvudsakligen i Karlskrona (civila inberäknade). Armén och Skärgårdsflottan uppges likadeles ha mist omkring 10.000 man, medan antalet i strid stupade armésoldater endast var ca 1500. Sammanlagt antas alltså ca 20.000 människor ha mist livet; både i återfallsfeber, men även i andra, samtidigt grasserande farsoter. I denna siffra är inte de övriga delarna av riket inberäknade. Epidemin i fråga kan alltså på goda grunder anses vara det svenska 1700-talets största medicinska katastrof.

Resistance Mechanisms of Non-Hodgkin Lymphomas against Rituximab Treatment

Rituximab, a monoclonal antibody against B-cell specific CD20 antigen, is used for the treatment of non-Hodgkin lymphomas (NHL) and chronic lymphatic leukemia. In combination with chemotherapeutics rituximab has remarkably improved the outcome of NHL patients, but a vast variation in the lengths of remissions remains and the outcome of individual patients is difficult to predict. This thesis has searched for an explanation for this by studying the effector mechanisms of rituximab and by comparing gene expression in lymphoma tissue samples of patients with long- and short-term survival. This work demonstrated that activation of complement (C) system is in vitro more efficient effector mechanism of rituximab than cellular mechanisms or apoptosis. Activation of the C system was also shown in vivo during rituximab treatment. However, intravenously administered rituximab could not enter the cerebrospinal fluid, and neither C activation nor removal of lymphoma cells was observed in central nervous system. In vitro cytotoxicity assays showed that rituximab-induced cell killing could be markedly improved with simultaneous neutralization of the C regulatory proteins CD46 (Membrane cofactor protein), CD55 (Decay-accelerating factor), and CD59 (protectin). In a retrospective study of follicular lymphoma (FL) patients, low lymphoma tissue mRNA expressions of CD59 and CD55 were associated with a good prognosis and in a progressive flow cytometry study high expression of CD20 relative to CD55 was correlated to a longer progression free survival. Gene expression profile analysis revealed that expression of certain often cell cycle, signal transduction or immune response related genes correlate with clinical outcome of FL patients. Emphasizing the role of tumor microenvironment the best differentiating genes Smad1 and EphA1 were demonstrated to be mainly expressed in the non-malignant cells of tumors. In conclusion, this thesis shows that activation of the C system is a clinically important effector mechanism of rituximab and that microenvironment factor in tumors and expression of C regulatory proteins affect markedly the efficacy of immunochemotherapy. This data can be used to identify more accurately the patients for whom immunochemotherapy is given. It may also be beneficial in development of rituximab-containing and other monoclonal antibody therapies against cancer.

Effect of phenolic-rich plant materials on protein and lipid oxidation reactions

The antioxidant activity of natural plant materials rich in phenolic compounds is being widely investigated for protection of food products sensitive to oxidative reactions. In this thesis plant materials rich in phenolic compounds were studied as possible antioxidants to prevent protein and lipid oxidation reactions in different food matrixes such as pork meat patties and corn oil-in water emulsions. Loss of anthocyanins was also measured during oxidation in corn oil-in-water emulsions. In addition, the impact of plant phenolics on amino acid level was studied using tryptophan as a model compound to elucidate their role in preventing the formation of tryptophan oxidation products. A high-performance liquid chromatography (HPLC) method with ultraviolet and fluorescence detection (UV-FL) was developed that enabled fast investigation of formation of tryptophan derived oxidation products. Byproducts of oilseed processes such as rapeseed (Brassica rapa L.), camelina (Camelina sativa) and soy meal (Glycine max L.) as well as Scots pine bark (Pinus sylvestris) and several reference compounds were shown to act as antioxidants toward both protein and lipid oxidation in cooked pork meat patties. In meat, the antioxidant activity of camelina, rapeseed and soy meal were more pronounced when used in combination with a commercial rosemary extract (Rosmarinus officinalis). Berry phenolics such as black currant (Ribes nigrum) anthocyanins and raspberry (Rubus idaeus) ellagitannins showed potent antioxidant activity in corn oil-in-water emulsions toward lipid oxidation with and without β-lactoglobulin. The antioxidant effect was more pronounced in the presence of β-lactoglobulin. The berry phenolics also inhibited the oxidation of tryptophan and cysteine side chains of β-lactoglobulin. The results show that the amino acid side chains were oxidized prior the propagation of lipid oxidation, thereby inhibiting fatty acid scission. In addition, the concentration and color of black currant anthocyanins decreased during the oxidation. Oxidation of tryptophan was investigated in two different oxidation models with hydrogen peroxide (H2O2) and hexanal/FeCl2. Oxidation of tryptophan in both models resulted in oxidation products such as 3a-hydroxypyrroloindole-2-carboxylic acid, dioxindolylalanine, 5-hydroxy-tryptophan, kynurenine, N-formylkynurenine and β-oxindolylalanine. However, formation of tryptamine was only observed in tryptophan oxidized in the presence of H2O2. Pine bark phenolics, black currant anthocyanins, camelina meal phenolics as well as cranberry proanthocyanidins (Vaccinium oxycoccus) provided the best antioxidant effect toward tryptophan and its oxidation products when oxidized with H2O2. The tryptophan modifications formed upon hexanal/FeCl2 treatment were efficiently inhibited by camelina meal followed by rapeseed and soy meal. In contrast, phenolics from raspberry, black currant, and rowanberry (Sorbus aucuparia) acted as weak prooxidants. This thesis contributes to elucidating the effects of natural phenolic compounds as potential antioxidants in order to control and prevent protein and lipid oxidation reactions. Understanding the relationship between phenolic compounds and proteins as well as lipids could lead to the development of new, effective, and multifunctional antioxidant strategies that could be used in food, cosmetic and pharmaceutical applications.

HPLC analysis of plant sterol oxidation products

Increased interest in the cholesterol-lowering effect of plant sterols has led to development of plant sterol-enriched foods. When products are enriched, the safety of the added components must be evaluated. In the case of plant sterols, oxidation is the reaction of main concern. In vitro studies have indicated that cholesterol oxides may have harmful effects. Due their structural similarity, plant sterol oxidation products may have similar health implications. This study concentrated on developing high-performance liquid chromatography (HPLC) methods that enable the investigation of formation of both primary and secondary oxidation products and thus can be used for oxidation mechanism studies of plant sterols. The applicability of the methods for following the oxidation reactions of plant sterols was evaluated by using oxidized stigmasterol and sterol mixture as model samples. An HPLC method with ultraviolet and fluorescence detection (HPLC-UV-FL) was developed. It allowed the specific detection of hydroperoxides with FL detection after post-column reagent addition. The formation of primary and secondary oxidation products and amount of unoxidized sterol could be followed by using UV detection. With the HPLC-UV-FL method, separation between oxides was essential and oxides of only one plant sterol could be quantified in one run. Quantification with UV can lead to inaccuracy of the results since the number of double bonds had effect on the UV absorbance. In the case of liquid chromatography-mass spectrometry (LC-MS), separation of oxides with different functionalities was important because some oxides of the same sterol have similar molecular weight and moreover epimers have similar fragmentation behaviour. On the other hand, coelution of different plant sterol oxides with the same functional group was acceptable since they differ in molecular weights. Results revealed that all studied plant sterols and cholesterol seem to have similar fragmentation behaviour, with only relative ion abundances being slightly different. The major advantage of MS detection coupled with LC separation is the capability to analyse totally or partly coeluting analytes if these have different molecular weights. The HPLC-UV-FL and LC-MS methods were demonstrated to be suitable for studying the photo-oxidation and thermo-oxidation reactions of plant sterols. The HPLC-UV-FL method was able to show different formation rates of hydroperoxides during photo-oxidation. The method also confirmed that plant sterols have similar photo-oxidation behaviour to cholesterol. When thermo-oxidation of plant sterols was investigated by HPLC-UV-FL and LC-MS, the results revealed that the formation and decomposition of individual hydroperoxides and secondary oxidation products could be studied. The methods used revealed that all of the plant sterols had similar thermo-oxidation behaviour when compared with each other, and the predominant reactions and oxidation rates were temperature dependent. Overall, these findings showed that with these LC methods the oxidation mechanisms of plant sterols can be examined in detail, including the formation and degradation of individual hydroperoxides and secondary oxidation products, with less sample pretreatment and without derivatization.

Lactic acid bacteria and their antimicrobial peptides : Induction,detection,partial characterization, and potential applications

Bacteriocin-producing lactic acid bacteria and their isolated peptide bacteriocins are of value to control pathogens and spoiling microorganisms in foods and feed. Nisin is the only bacteriocin that is commonly accepted as a food preservative and has a broad spectrum of activity against Gram-positive organisms including spore forming bacteria. In this study nisin induction was studied from two perspectives, induction from inside of the cell and selection of nisin inducible strains with increased nisin induction sensitivity. The results showed that a mutation in the nisin precursor transporter NisT rendered L. lactis incapable of nisin secretion and lead to nisin accumulation inside the cells. Intracellular proteolytic activity could cleave the N-terminal leader peptide of nisin precursor, resulting in active nisin in the cells. Using a nisin sensitive GFP bioassay it could be shown, that the active intracellular nisin could function as an inducer without any detectable release from the cells. The results suggested that nisin can be inserted into the cytoplasmic membrane from inside the cell and activate NisK. This model of two-component regulation may be a general mechanism of how amphiphilic signals activate the histidine kinase sensor and would represent a novel way for a signal transduction pathway to recognize its signal. In addition, nisin induction was studied through the isolation of natural mutants of the GFPuv nisin bioassay strain L. lactis LAC275 using fl uorescence-activated cell sorting (FACS). The isolated mutant strains represent second generation of GFPuv bioassay strains which can allow the detection of nisin at lower levels. The applied aspect of this thesis was focused on the potential of bacteriocins in chicken farming. One aim was to study nisin as a potential growth promoter in chicken feed. Therefore, the lactic acid bacteria of chicken crop and the nisin sensitivity of the isolated strains were tested. It was found that in the crop Lactobacillus reuteri, L. salivarius and L. crispatus were the dominating bacteria and variation in nisin resistance level of these strains was found. This suggested that nisin may be used as growth promoter without wiping out the dominating bacterial species in the crop. As the isolated lactobacilli may serve as bacteria promoting chicken health or reducing zoonoosis and bacteriocin production is one property associated with probiotics, the isolated strains were screened for bacteriocin activity against the pathogen Campylobacter jejuni. The results showed that many of the isolated L. salivarius strains could inhibit the growth of C. jejuni. The bacteriocin of the L. salivarius LAB47 strain, with the strongest activity, was further characterized. Salivaricin 47 is heat-stable and active in pH range 3 to 8, and the molecular mass was estimated to be approximately 3.2 kDa based on tricine SDS-PAGE analysis.

Properties of Ammonium Nitrate based fertilisers

The text is divided into three parts; Properties, Application and Safety of Ammonium Nitrate (AN) based fertilisers. In Properties, the structures and phase transitions of ammonium and potassium nitrate are reviewed. The consequences of phase transitions affect the proper use of fertilisers. Therefore the products must be stabilised against the volume changes and consequent loss of bulk density and hardness, formation of dust and finally caking of fertilisers. The effect of different stabilisers is discussed. Magnesium nitrate, ammonium sulphate and potassium nitrate are presented as a good compromise. In the Application part, the solid solutions in the systems (K+,NH4+)NO3- and (NH4+,K+)(Cl-,NO3-) are presented based on studies made with DSC and XRD. As there are clear limits for solute content in the solvent lattice, a number of disproportionation transitions exist in these process phases, e.g., N3 (solid solution isomorphous to NH4NO3-III) disproportionates to phases K3 (solid solution isomorphous to KNO3-III) and K2 (solid solution isomorphous to KNO3-II). In the crystallisation experiments, the formation of K3 depends upon temperature and the ratio K/(K+NH4). The formation of phases K3, N3, and K2 was modelled as a function of temperature and the mole ratios. In introducing chlorides, two distinct maxima for K3 were found. Confirmed with commercial potash samples, the variables affecting the reaction of potassium chloride with AN are the particle size, time, temperature, moisture content and amount of organic coating. The phase diagrams obtained by crystallisation studies were compared with a number of commercial fertilisers and, with regard to phase composition, the temperature and moisture content are critical when the formation and stability of solid solutions are considered. The temperature where the AN-based fertiliser is solidified affects the amount of compounds crystallised at that point. In addition, the temperature where the final moisture is evaporated affects the amount and type of solid solution formed at this temperature. The amount of remaining moisture affects the stability of the K3 phase. The K3 phase is dissolved by the moisture and recrystallised into the quantities of K3, which is stable at the temperature where the sample is kept. The remaining moisture should not be free; it should be bound as water in the final product. The temperatures during storage also affect the quantity of K3 phase. As presented in the figures, K3 phase is not stable at temperatu¬res below 30 °C. If the temperature is about 40 °C, the K3 phase can be formed due to the remaining moisture. In the Safety part, self-sustaining decomposition (SSD), oxidising and energetic properties of fertilisers are discussed. Based on the consequence analysis of SSD, early detection of decomposition in warehouses and proper temperature control in the manufacturing process is important. SSD and oxidising properties were found in compositions where K3 exists. It is assumed that potassium nitrate forms a solid matrix in which AN can decompose. The oxidising properties can be affected by the form of the product. Granular products are inherently less oxidising. Finally energetic properties are reviewed. The composition of the fertiliser has an importance based on theoretical calculations supported by experimental studies. Materials such as carbonates and sulphates act as diluents. An excess of ammonium ions acts as a fuel although this is debatable. Based on the experimental work, the physical properties have a major importance over the composition. A high bulk density is of key importance for detonation resistance.

Beatific Enjoyment in Scholastic Philosophy and Theology: 1240-1335

This dissertation examines the concept of beatific enjoyment (fruitio beatifica) in scholastic theology and philosophy in the thirteenth and early fourteenth century. The aim of the study is to explain what is enjoyment and to show why scholastic thinkers were interested in discussing it. The dissertation consists of five chapters. The first chapter deals with Aurelius Augustine's distinction between enjoyment and use and the place of enjoyment in the framework of Augustine's view of the passions and the human will. The first chapter also focuses upon the importance of Peter Lombard's Sentences for the transmission of Augustine's treatment of enjoyment in scholastic thought as well as upon Lombard's understanding of enjoyment. The second chapter treats thirteenth-century conceptions of the object and psychology of enjoyment. Material for this chapter is provided by the writings - mostly Sentences commentaries - of Alexander of Hales, Albert the Great, Bonaventure, Thomas Aquinas, Peter of Tarentaise, Robert Kilwardby, William de la Mare, Giles of Rome, and Richard of Middleton. The third chapter inspects early fourteenth-century views of the object and psychology of enjoyment. The fourth chapter focuses upon discussions of the enjoyment of the Holy Trinity. The fifth chapter discusses the contingency of beatific enjoyment. The main writers studied in the third, fourth and fifth chapters are John Duns Scotus, Peter Aureoli, Durandus of Saint Pourçain, William of Ockham, Walter Chatton, Robert Holcot, and Adam Wodeham. Historians of medieval intellectual history have emphasized the significance of the concept of beatific enjoyment for understanding the character and aims of scholastic theology and philosophy. The concept of beatific enjoyment was developed by Augustine on the basis of the insight that only God can satisfy our heart's desire. The possibility of satisfying this desire requires a right ordering of the human mind and a detachment of the will from the relative goals of earthly existence. Augustine placed this insight at the very foundation of the notion of Christian learning and education in his treatise On Christian Doctrine. Following Augustine, the twelfth-century scholastic theologian Peter Lombard made the concept of enjoyment the first topic in his plan of systematic theology. The official inclusion of Lombard's Sentences in the curriculum of theological studies in the early universities stimulated vigorous discussions of enjoyment. Enjoyment was understood as a volition and was analyzed in relation to cognition and other psychic features such as rest and pleasure. This study shows that early fourteenth-century authors deepened the analysis of enjoyment by concentrating upon the relationship between enjoyment and mental pleasure, the relationship between cognition and volition, and the relationship between the will and the beatific object (i.e., the Holy Trinity). The study also demonstrates the way in which the idea of enjoyment was affected by changes in the method of theological analysis - the application of Aristotelian logic in a Trinitarian context and the shift from virtue ethics to normative ethics.

Power and Authority. Birgitta of Sweden and her Revelations

In the High Middle Ages female saints were customarily noble virgins. Thus, as a wife and a mother of eight children, the Swedish noble lady Birgitta (1302/3 1373) was an atypical candidate for sanctity. However, in 1391 she was canonized only 18 years after her death and became a role model for many late medieval women, who were mothers and widows. The dissertation Power and Authority Birgitta of Sweden and Her Revelations investigates how Birgitta went about establishing her power and authority during the first ten years of her career as a living saint, in 1340 1349. It is written from the perspectives of gender, authority, and power. The sources consist of approximately seven hundred revelations, hagiographical texts and other medieval documents. This work concentrates on the interaction between Birgitta and her audience. During her lifetime Birgitta was already regarded as a holy woman, as a living saint. A living saint could be given no formal papal or other recognition, for one could never be certain about his or her future activities. Thus, the living saint needed an audience for whom to perform signs of sanctity. In this study particular attention is paid to situations within which the power relations between the living saint and her audience can be traced and are open to critical analysis. Situations of conflict that arose in Birgitta s life are especially fruitful for this purpose. During the Middle Ages, institutional power and authority were exclusively in the hands of secular male leaders and churchmen. In this work it is argued, however, that Birgitta used different kinds of power than men. It is evident that she exercized influence on lay people as well as on secular and clerical authorities. The second, third, and fourth chapter of this study examine the beginning of Birgitta s career as a visionary, what factors and influences lay behind it, and what kind of roles they played in establishing her religious authority. The fifth, sixth, and seventh chapter concentrate on Birgitta s exercising of power in specific situations during her time in Sweden until she left on a pilgrimage to Rome in 1349. The central question is how she exercised power with different people. As a result, this book will offer a narrative of Birgitta s social interactions in Sweden seen from the perspectives of power and authority. Along with the concept of power, authority is a key issue. By definition, one who has power also has authority but a person who does not have official power can, nevertheless, have authority. Authority in action is defined here as meaning that a person was listened to. Birgitta acted both in situations of open conflict and where no conflict was evident. Her strategies included, for example, inducement, encouragement and flattery. In order to make people do as she felt was right she also threatened them openly with divine wrath. Sometimes she even used both positive persuasion and threats. Birgitta s power seems very similar to that of priests and ascetics. Common to all of them was that their power demanded interaction with other people and audiences. Because Birgitta did not have power and authority ex officio she had to persuade people to believe in her powers. She did this because she was convinced of her mission and sought to make people change their lives. In so doing, she moved from the domestic field to the public fields of religion and politics.

Martta vai Maria? : Aktiivisen ja kontemplatiivisen välinen suhde Mestari Eckhartin mystiikanteologiassa.

Tämän tutkimuksen tarkoitus on selvittää millainen suhde käsitteiden ”aktiivinen” ja ”kontemplatiivinen” välillä vallitsee 1200- ja 1300-lukujen taitteessa eläneen saksalaisen teologin Mestari Eckhartin mystiikanteologiassa. Lisäksi on tarkoitus selvittää mitä tuon suhteen kautta on mahdollista paljastaa Eckhartin mystiikanteologiasta laajemmin. Tutkimuksen metodi on systemaattinen analyysi ja päälähteinä käytetään Eckhartin saksankielisiä saarnoja. Erityisesti analysoidaan Eckhartin Martta-Maria -perikooppia (Lk.10:38–42) käsitteleviä saarnoja Pr.86 ja Pr.2. Lisäksi lähteinä käytetään muita Eckhartin saksankielisiä saarnoja ja muuta tuotantoa siinä määrin kuin niiden mukaan ottaminen auttaa paremmin ymmärtämään saarnojen intentioita. Jo ensimmäisistä kristillisistä vuosisadoista lähtien Luukkaan evankeliumin kertomus Martasta ja Mariasta on ollut aktiivisen ja kontemplatiivisen elämän locus classicus. Siksi tutkimuskysymykseen vastaamiseksi on valittu juuri Eckhartin sitä käsittelevät saarnat. Itse tutkimuskysymyksen valintaan on vaikuttanut puolestaan se, että Eckhart tulkitsee perikooppia häntä edeltävästä tulkintatraditiosta selvästi poikkeavalla tavalla ja nostaa Martan sisaruksista edistyneemmäksi. Tutkimus etenee seuraavalla tavalla. Luvussa 2 avataan käsitteiden ”aktiivinen” ja ”kontemplatiivinen” sisältöä sekä käsitellään kysymystä siitä miten niiden välistä suhdetta on kirkon historiassa tulkittu. Se tapahtuu esittelemällä kolme keskeistä tulkintalinjaa: normaalikirkollinen, monastinen ja mendikanttisääntökuntien tulkinta. Luvussa 3 käydään läpi Eckhartin elämää, tuotantoa ja harhaoppisuustuomiota sekä hänen teologiansa keskeisiä jäsentäviä periaatteita. Koska Eckhart on suomalaiselle yleisölle verrattain tuntematon teologi, aihetta käsitellään hieman laajemmin kuin pro gradu -tukielmassa on yleensä tapana. Luvussa 4 perehdytään Martta-Maria -perikooppiin ja sen pitkään tulkintahistoriaan. Luvussa 5 analysoidaan Eckhartin saarna Pr.86, luvussa 6 Pr.2. Luvussa 7 tutkimuksen tulokset kootaan yhteen. Eckhartin saarnoja analysoimalla paljastuu, että hän tulkitsee aktiivisen ja kontemplatiivisen elämän suhteen häntä edeltävästä traditiosta poikkeavalla tavalla. Eckhartia edeltävänä aikana kontemplatiivisia pyrkimyksiä ja Mariaa oli pidetty kristillisessä kirkossa aktiivista elämää ihailtavampana ja ansiollisempana jo tuhat vuotta. Siihen oli vaikuttanut erityisesti se, että 400-luvulta lähtien aina 1200-luvulle asti luostarit olivat Länsi-Euroopan sivistyksen ja teologian keskuksia. Sen vuoksi juuri monastisia ihanteita – erityisesti kontemplatiivisia pyrkimyksiä Jumalan välittömään katselemiseen – pidettiin pitkään kristillisen elämän ihanteina. Eckhart on kuitenkin osa uutta tulkintatraditiota jossa perinteisen klausuurin rajat pyrittiin laajentamaan luostarin muureista koskemaan koko maailmaa. Tämä uusi tulkintatraditio oli ominaista erityisesti mendikanttisääntökunnille (fransiskaanit ja dominikaanit) sekä begiiniyhteisöille, joissa kontemplatiivinen ja aktiivinen elämä pyrittiin yhdistämään yhdeksi kristilliseksi elämäksi. Tutkimuksessa käy ilmi, että suhteessa laajempiin tulkintatradition linjoihin, Eckhart voidaan liittää luontevasti osaksi näiden sääntökuntien uusia orastavia tulkintoja. Niiden mukaan kristillistä täydellisyyttä ei tavoiteta kääntymällä pois päin maailmasta vaan sitä kohti. Sen on kuitenkin tarkoitus tapahtua niin, että aktiivisuus maailmassa nousee kontemplatiivisesta elämänmuodosta käsin. Toisaalta tutkimuksessa paljastuu myös, että Eckhart menee vielä edellä mainittuja uusia tulkintojakin pidemmälle. Mendikanttisääntökunnissa kontemplatiivisuus säilytti yleensä vielä jonkinlaisen ensisijaisuuden aktiivisuuteen nähden, siihen valmistavana ja sen mahdollistavana vaiheena. Eckhart saarnaa kuitenkin niiden täydellisen ykseyden ja samanaikaisuuden puolesta. Hänelle täydellisyyttä edustaa Betanian sisaruksista Martta joka on ”neitsyt-vaimo”, koskematon ja puhdas, mutta joka kuitenkin samalla synnyttää. Neitsyyden ja vaimouden täydellisen samanaikaisuuden korostaminen nousee puolestaan Eckhartin voimakkaasta sitoutumisesta uusplatoniseen metafysiikkaan. Eckhartin ontologia on ”dynaamista ontologiaa”, jossa kaikki ymmärretään Jumalan yliajalliseksi emanaatioksi. Tulkitsemalla mm. Proklosta, Eckhart esittää, että kaikki luotu on olemassa ensisijaisesti ja täydellisenä ikuisuudessa syntyvässä Pojassa ja vain formaalisesti ja vajavaisella tavalla havaittavassa todellisuudessa. Kristillinen täydellisyys on Eckhartille paluuta tuohon varsinaiseen olemiseen ikuisuudessa syntyvässä Pojassa. Paluu tapahtuu irrottautumalla (abegescheiden) kuvista (bilde) eli hyväksymällä, että Jumala ei ole missään käsitteissä, eikä häntä tavoiteta samoin kuin olemassa olevat asiat tavoitetaan. Sikäli kun ihminen riisutaan riippuvuudestaan kuvista, sikäli hän murtautuu (durchbrechen) takaisin siihen perustaan (grunt) jossa Poika ikuisesti syntyy. Perustasta käsin ihmisen on puolestaan mahdollista synnyttää sellaista aktiivisuutta ja toimintaa, joka ei ole itsekkyyden ja egon tahraamaa suorittamista ja kaupankäyntiä. Tutkimuksessa käy ilmi myös se, kuinka keskeinen rooli ymmärryksen (intellectus) kategorialla on kuvista luopumisen prosessissa ja Jumalan kaltaisuuden realisoitumisessa ihmisessä. Juuri ymmärtämisaktin (intelligere) puolesta ihminen on Eckhartin mukaan eniten Jumalan kaltainen ja osa Jumalan ikuisuudessa tapahtuvaa emanaatiota.