鳜肿瘤坏死因子相关的凋亡诱导配体Trail基因的原核表达

中国科学院“百人计划”项目([2005]192)资助

TRAIL in the mandarin fish Siniperca chuatsi: Gene and its apoptotic effect in HeLa cells

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is one of the TNF superfamily members, participating in many biological processes including cell proliferation and apoptotic death. In this study, a TRAIL gene was cloned from a perciform fish, the mandarin fish Siniperca chuatsi, a major cultured fish in China's aquaculture, and is named as SCTRAIL for S. chuatsi TRAIL. The full-length cDNA of SCTRAIL is 1359 bp, encoding a 283-amino-acid protein. This deduced protein contains the CYS231, a 23-mer fragment of transmembrane region, a glycosylation site and a TNF family signature, all of which are conserved among TRAIL members. SCTRAIL gene consists of six exons, with five intervening introns, spaced over approximately 9 kb of genomic sequence. Southern blotting demonstrated that the SCTRAIL gene is present as a single copy in mandarin fish genome. A 620 bp promoter region obtained by genome walking contains a number of putative transcription factor binding sites, such as Oct-1, Sp-1, NF-1, RAP-1, C/EBPaLp, NF-kappa B and AP-1. The SCTRAIL is constitutively expressed in all the analyzed tissues, as revealed by RT-PCR, which is confirmed by Western blotting analysis using polyclonal antibody against bacteria-derived recombinant SCTRAIL protein. As an apoptosis-inducing ligand, the overexpression of SCTRAIL but not the mutant SCTRAIL-C203S in HeLa cells induced changes characteristic of apoptosis, including chromatin condensation, nucleus fragmentation, DNA ladder, and increase of sub-G0/G1 cells in FACS analysis. (c) 2007 Elsevier Ltd. All rights reserved.

Characterization and expression analysis of TNF-related apoptosis inducing ligand (TRAIL) in grass carp Ctenopharyngodon idella

TRAIL (Apo2 ligand) described as a type II transmembrame protein belonging to the TNF superfamily can induce apoptotic cell death in a variety of cell types. In the present study, a putative cDNA sequence encoding the 299 amino acids of TRAIL (GC-TRAIL) and its genomic organization were identified in grass carp Ctenopharyngodon idella. The predicted GC-TRAIL sequence showed 44 and 41% identities to chicken and human TRAILs, respectively. In a domain search, a tumor necrosis factor homology domain (THD) was identified in the C-terminal portion of TRAILs. The GC-TRAIL gene consists of five exons, with four intervening introns, spaced over approximately 4 kb of genomic sequence. Analysis of GC-TRAlL promoter region revealed the presence of a number of putative transcription factor binding sites, such as Sp1, NF-kappaB, AP-1, GATA, NFAT, HNF, STAT, P53 and IRFI sequences which are important for the expression of other TNF family members. Phylogenetic analysis placed GC-TRAIL and the putative zebrafish (Danio rerio) TRAIL obtained from searching the zebrafish database into one separate cluster near mammalian TRAIL genes, but apart from the reported zebrafish TRAIL-like protein, indicating that the GC-TRAIL is an authentic fish TRAIL. Expression analysis revealed that GC-TRAIL is expressed in many tissues, such as in gills, liver, trunk kidney, head kidney, intestine and spleen. (c) 2005 Elsevier B.V. All rights reserved.

Median network analysis of defectively sequenced entire mitochondrial genomes from early and contemporary disease studies

Sequence analysis of the mitochondrial genome has become a routine method in the study of mitochondrial diseases. Quite often, the sequencing efforts in the search of pathogenic or disease-associated mutations are affected by technical and interpretive pr

SEXUAL-BEHAVIOR OF TIBETAN MACAQUES AT MT EMEI, CHINA

Data on sexual behavior were collected in six groups of semi-commensal Macaca thibetana along the trail on the slope habitat between 1987 and 1989. Ignoring the common items such as mounting, presenting etc., 20 categories of sexual behavior were described. Most of the descriptions were likely to have enlarged the behavior repertoire reported in macaques, showing a great complexity of sociosexual interactions under the principally natural condition. A great diversity of grouping appeared in the mating season. The copulatory pattern was found to be the serial type contrary to previous speculation, and the mount-to-ejaculation ratio was higher in the central subgroup, as compared with the far-peripheral adult subgroup (FAS) with less male and female rivals. An age-class subdivision of sexually active males made it possible to show that the young adult male immigrants were the most active class in sexual activity. Subgrouping form FAS was a ''space-segregation'' tactic of mating for the losers of both sexes in the competition. Some parameters of copulation were also documented.

Intergroup interactions in Tibetan macaques at Mt. Emei, China

Data on intergroup-interactions (I-I) were collected in 5 seasonally provisioned groups (A, B, D, D-1, and E) of Tibetan macaques (Macaca Thibetana) at Mt. Emei in three 70-day periods between 1991 April-June (P1), September-November (P2), December-1992 February (P3). The I-I were categorized as forewarning made by high-ranking males (including Branch Shaking and/or Loud Calls), long-distance interactions in space (specified by changes in their foraging movements), and close encounters (with Affinitive Behavior, Male's Herding Female, Sexual Interaction, Severe Conflict, Adult Male-male Conflict, Opportunistic Advance and Retreat, etc. performed by different age-sex classes). From periods Fl to P3, the I-I rate decreased with reduction in population density as a positive correlate of food clumpedness or the number of potential feeders along a pedestrian trail. On the other hand, from the birth season (BS, represented by P1 and P3) to the mating season (MS, represented by P2) the dominance relation between groups, which produced a winner and a loser in the encounters, became obscure; the proportion of close encounters in the I-I increased; the asymmetry (local groups over intruders) of forewarning signals disappeared; the rate of branch shaking decreased; and sometimes intergroup cohesion appeared. Considering that sexual interactions also occurred between the encountering groups, above changes in intergroup behaviors may be explained with a model of the way in which the competition for food (exclusion) and the sexual attractiveness between opposite sexes were in a dynamic equilibrium among the groups, with the former outweighing the latter in the BS, and conversely in the MS. Females made 93% of severe conflicts, which occurred in 18% of close encounters. Groups fissioned in the recent past shared the same home range, and showed the highest hostility to each other by females. In conspicuous contrast with females' great interest in intergroup food/range competition, adult male-male conflicts that were normally without body contact occurred in 66% bf close encounters; high-ranking male herding of females, which is typical in baboons, appeared in 83% of close encounters, and showed no changes with season and sexual weight-dimorphism; peripheral juvenile and subadult males were the main performers of the affinitive behaviors, opportunistic advance and retreat, and guarding at the border. In brief, all males appeared to "sit on the fence" at the border, likely holding out hope of gaining the favor of females both within and outside the group. Thus, females and males attempted to maximize reproductive values in different ways, just as expected by Darwin-Trivers' theory of sexual selection. In addition, group fission was observed in the largest and highest-ranking group for two times (both in the MS) when its size increased to a certain level, and the mother group kept their dominant position in size and rank among the groups that might encounter, suggesting that fission takes a way of discarding the "superfluous part" in order to balance the cost of competition for food and mates within a group, and the benefit of cooperation to access the resources for animals in the mother group. (C) 1997 Wiley-Liss, Inc.

Responses to seasonal changes in nutrient quality and patchiness of food in a multigroup community of Tibetan macaques at Mt. Emei

Provisioning along pedestrian trails by tourists much increased the nutrient quality and patchiness of food (NqPF)for Tibetan macaques (Macaca thibetana) at Mt Emei in spring and summer. In the habitat at a temperate-subtropical transition zone, the mncaque's NqPF could be ordered in a decreasing rank from spring summer to autumn to winter With the aid of a radio-tracking system, I collected ranging data on a multigroup community in three 70-day periods representing the different seasons in 1991-92, Rank-order correlation on the data show that with the decline of NqPF; the groups tended to increase days away from the trail, their effective range size (ERS) their exclusive area (EA) and the number of days spent in the EA, and reduced their group/community density and the ratio of the overlapped range to the seasonal range (ROR). In icy/snowy winter; the macaques searched for mature leaves slowly and carefully in the largest seasonal range with a considerable portion that was nor used in other seasons. Of the responses, the ROR decreased with the reduction in group/community density; and the ERS was the function of both group size (+) and intergroup rank (-) when favorite food was highly clumped. All above responses were clearly bound to maximize foraging effectiveness and minimize energy expenditure, and their integration in term of changes in time and space leads to better understanding macaque ecological adaptability. Based on this study and previous work on behavioral and physiological factors, I suggest a unifying theory of intergroup interactions. Ir! addition, as the rate of behavioral interactions,was also related to the group density, I Waser's (1976) gas model probably applies to behavioral, as well as spatial, data on intergroup interactions.

两种抗HER2重组免疫毒素的构建与活性研究

HER2／neu基因在肿瘤中的过度表达使其成为许多肿瘤的标志分子。人肿瘤坏死因子（TNF-α）和肿瘤坏死因子相关的凋亡诱导配体（Trail）对肿瘤细胞的杀伤作用使其成为前景看好的抗肿瘤药物，对它们的细胞杀伤机制研究日渐深入。但临床研究发现HER2／neu过度表达的肿瘤细胞抵制TNF-α和Trail的肿瘤杀伤作用，因此经常产生耐药现象。为了增加过度表达HER2／neu的肿瘤细胞对TNF-a的敏感性和提高HER2／neu抗体的肿瘤杀伤效应，我们将抗HER2／neu人源化单链抗体scFvC6.5与人翔F-a融合，构建了免疫毒素scFvC6.5-TNF-α，完成了该重组蛋白在大肠杆菌中的表达，产率为800μg／L菌液。经过亲和层析和柱复性，融合蛋白的纯度达95％以上。ELISA试验表明scFvC6.5-TNF-a能够特异结合HER2／neu阳性卵巢癌细胞SKO从3和乳腺癌细胞MCF-7，而不结合HERZ／neu阴性的黑色素瘤细胞A-375。MTT试验表明scFvC6.5-TNF-a能够选择性的杀伤SKOV-3和MCF-7细胞，而不影响A-375细胞的生长。同时为了增加过度表达HER2／neu的肿瘤细胞对人可溶性肿瘤坏死因子相关的凋亡诱导配体（sTrail）的敏感性和提高HER2／neu抗体的肿瘤杀伤效应，我们构建了scFvC6．5与人sTrail的融合蛋白scFvC6.5-sTrail。重组子经酶切及测序证明序列正确后，在大肠杆菌BL21（DE3）中进行诱导表达。经SDS-PAGE及westem一blot鉴定，获得高水平包含体表达菌株，产率为700雌／L菌液。对表达产物进行变性、复性及纯化，SDS-PAGE结果显示纯度达95％以上。用ELISA法检测纯化后蛋白的结合活性表明融合蛋白scFvC6.5-sTrail能够特异结合HERZ／neu阳性卵巢癌细胞SKO从3、乳腺癌细胞McF-7和Trail敏感菌株MDA-MB-231，而不结合HER2／neu阴性和Trail受体阴性的黑色素瘤细胞A-375。MTT法检测其生物活性显示纯化后的scFvC6.5-sTrail蛋白对SKO从3、MCF-7、MDA-MB-231均具有细胞毒活性，并存在剂量依赖性，但对A-375细胞没有作用。细胞凋亡流式分析表明这两种免疫毒素对SKO从3靶细胞的杀伤作用是通过诱导细胞凋亡所致。提示这两种免疫毒素在抗肿瘤靶向治疗中具有潜在的应用价值。

海拉尔盆地贝尔凹陷基岩潜山特征及储层预测

The lithology of the buried hill of Triassic Budate group in Beier depression is epimetamorphic clastic rock and volcanic clastic rock stratum. Recently the favorable hydrocarbon show was discovered in buried hill of base rock, and large-duty industrial oil stream was obtained in some wells in Beier depression. Based on the information of seismos and wells, the tectonic framework, tectonic deformation times and faulted system of the Beier depression are comprehensively studied, then configuration, evolutional history, genetic type and distributed regularity of buried hill are defined. According to observing description and analysis of core sample, well logging and interpretive result of FMI, the lithological component, diagenetic type and diagenetic sequence of buried hill reservoir are confirmed, then reservoir space system of buried hill is distinguished, and vegetal feature, genetic mechanism and distributed regularity of buried hill fissure are researched, at the same time the quantitative relationship is build up between core fissures and fissures interpreted by FMI. After that fundamental supervisory action of fault is defined to the vegetal degree of fissure, and the fissure beneficial places are forecasted using fractal theory and approach. At last the beneficial areas of Budate group reservoir are forecasted by reservoir appraisal parameters optimization such as multivariate gradually regression analysis et. al. and reservoir comprehensive appraisal method such as weighing analyze and clustering procedure et. al. which can provide foundation for the next exploratory disposition. Such production and knowledge are obtained in this text as those: 1. Four structural layers and two faulting systems are developed, and four structural layers are carved up by three bed succession boundary surfaces which creates three tectonic distortional times homology. Three types of buried hill are divided, they are ancient physiognomy buried hill, epigenetic buried hill, and contemporaneous buried hill. 2. Reservoir space of Budate buried hill is mainly secondary pore space and fissure, which distributes near the unconformity and/or inside buried hill in sections. The buried hill reservoir experienced multi-type and multi-stage diagenetic reconstruction, which led to the original porosity disappeared, and multi secondary porosity was created by dissolution, superficial clastation and cataclasis et. al. in diagenetic stage, which including middle crystal pore, inter crystal pore, moldic pore, inter particle emposieu, corrosion pore space and fissure et. al. which improved distinctly reservoir capability of buried hill. 3. The inner reservoir of buried hill in Beier depression is not stratigraphic bedded construction, but is fissure developing place formed by inner fault and broken lithogenetic belt. The fissures in inner reservoir of buried hill are developed unequally with many fissure types, which mainly are high angle fissure and dictyonal fissures and its developing degree and distribution is chiefly controlled by faulting. 4. The results of reservoir comprehensive evaluate and reservoir predicting indicates that advantageous areas of reservoir of buried hill chiefly distributes in Sudeerte, Beixi and Huoduomoer, which comprehensive evaluate mainly Ⅱand Ⅲ type reservoir. The clues and results of this text have directive significance for understanding the hydrocarbon reservoir condition of buried hill in Beier depression, for studying hydrocarbon accumulated mechanism and distributed regularity, and for guiding oil and gas exploration. The results of this text also can enrich and improve nonmarine hydrocarbon accumulated theory.

复杂地质条件下高精度地震勘探技术研究与应用-以泌阳凹陷为例

In order to carry out high-precision three-dimensional "integration" for the characteristics of the secondary seismic exploration for Biyang Depression, in the implementation process, through a combination of scientific research and production, summed up high-precision seismic acquisition, processing and interpretation technologies suitable for the eastern part of the old liberated areas, achieved the following results: 1. high-precision complex three-dimensional seismic exploration technology series suitable for shallow depression Biyang block group. To highlight the shallow seismic signal, apply goal-based observing system design, trail from the small panel to receive and protect the shallow treatment of a range of technologies; to explain the use of three-dimensional visualization and coherent combination of full-body three-dimensional fine interpretation identification of the 50-100 m below the unconformity surface and its formation of about 10 meters of the distribution of small faults and improve the small block and stratigraphic unconformity traps recognition. 2. high-precision series of three-dimensional seismic exploration technology suitable for deep depression Biyang low signal to noise ratio of information. Binding model using forward and lighting technology, wide-angle observation system covering the design, multiple suppression and raise the energy of deep seismic reflection processing and interpretation of detailed, comprehensive reservoir description, such as research and technology, identified a number of different types of traps. 3. high-precision seismic exploration technology series for the southern Biyang Depression high steep three-dimensional structure. The use of new technology of seismic wave scattering theory and high-precision velocity model based on pre-stack time migration and depth migration imaging of seismic data and other high-precision processing technology, in order to identify the southern steep slope of the local structure prediction and analysis of sandstone bedrock surface patterns provide a wealth of information.

东海陆架盆地西南部烟囱构造与油气运聚关系研究

In recent years, chimney structure has been proved one of important indicators and a useful guide to major petroleum fields exploration through their exploration history both at home and abroad. Chimney structure, which has been called "gas chimney" or "seismic chimney", is the special fluid-filled fracture swarm, which results from the boiling of active thermal fluid caused by abruptly decreasing of high pressure and high temperature in sedimentary layers of upper lithosphere. Chimney structure is well developed in continental shelf basin of East China Sea, which indicates the great perspectives of petroleum resources there. However, the chimney structure also complicated the petroleum accumulation. So the study of chimney structure on its formation, its effect on occurrence and distribution of petroleum fields is very important not only on theoretical, but also on its applied research. It is for the first time to make a clear definition of chimney structure in this paper, and the existence and practical meaning of chimney structure are illustrated. Firstly, on the viewpoint of exploration, this will amplify exploration area or field, not only in marine, but also on continent. Secondly, this is very important to step-by-step exploration and development of petroleum fields with overpressure. Thirdly, this will provide reference for the study on complex petroleum system with multi-sources, commingled sources and accumulation, multi-stage accumulations, and multi-suits petroleum system in the overlay basin. Fourthly, when the thermal fluid enters the oceanic shallow layer, it can help form gas hydrate under favorable low-temperature and high-pressure conditions. Meanwhile, the thermal fluid with its particular component and thermal content will affect the physical, chemical and ecological environments, which will help solving the problem of global resources and environment. Beginning from the regional tectonic evolution characteristics, this paper discussed the tectonic evolution history of the Taibei depression, then made an dynamical analysis of the tectonic-sedimentary evolution during the Mesozoic and Cenozoic for the East China Sea basin. A numerical model of the tectonic-thermal evolution of the basin via the Basin-Mod technique was carried out and the subsidence-buried history and thermal history of the Taibei depression were inverse calculated: it had undergone a early rapid rift and sag, then three times of uplift and erosion, and finally depressed and been buried. The Taibei depression contains a huge thick clastic sedimentary rock of marine facies, transitional facies and continental facies on the complex basement of ante-Jurassic. It is a part of the back-arc rifting basins occurred during the Mesozoic and Cenozoic. The author analyzed the diagenesis and thermal fluid evolution of this area via the observation of cathodoluminescence, scanning electron microscope and thin section, taking advantage of the evidences of magma activities, paleo-geothermics and structural movement, the author concluded that there were at least three tectonic-thermal events and three epochs of thermal-fluid activities; and the three epochs of thermal-fluid activities were directly relative to the first two tectonic-thermal events and were controlled by the generation and expulsion of hydrocarbon in the source rock simultaneously. Based on these, this paper established the corresponding model between the tectonic-thermal events and the thermal-fluid evolution of the Taibei Depression, which becomes the base for the study on the chimney structures. According to the analyses of the gas-isotope, LAM spectrum component of fluid inclusion, geneses of CO_2 components and geneses of hydrocarbon gases, the author preliminarily verified four sources of the thermal fluid in the Taibei Depression: ① dehydration of mud shale compaction, ② expulsion of hydrocarbon in the source rock; ③ CO_2 gas hydro-thermal decomposition of carbonatite; ④magma-derived thermal fluid including the mantle magma water and volatile components (such as H_2O, CO_2, H_2S, SO_2, N_2 and He etc.). On the basis of the vitrinite reflectance (Ro), homogenization temperature of fluid inclusion, interval transit time of major well-logging, mud density of the wells, measured pressure data and the results of previous studies, this paper analyzed the characteristics of the geothermal fields and geo-pressure fields for the various parts in this area, and discussed the transversal distribution of fluid pressure. The Taibei depression on the whole underwent a temperature-loss process from hot basin to cold basin; and locally high thermal anomalies occurred on the regional background of moderate thermal structure. The seal was primarily formed during the middle and late Paleocene. The overpressured system was formed during the middle and late Eocene. The formation of overpressured system in Lishui Sag underwent such an evolutionary process as "form-weaken-strengthen-weaken". Namely, it was formed during the middle and late Eocene, then was weakened in the Oligocene, even partly broken, then strengthened after the Miocene, and finally weakened. The existence of the thermal fluid rich in volatile gas is a physical foundation for the boiling of the fluid, and sharply pressure depletion was the major cause for the boiling of the fluid, which suggests that there exists the condition for thermal fluid to boil. According to the results of the photoelastic simulation and similarity physical experiments, the geological condition and the formation mechanism of chimnestructures are summarized: well compartment is the prerequisite for chimney formation; the boiling of active thermal fluid is the original physical condition for chimney formation; The local place with low stress by tension fault is easy for chimney formation; The way that thermal fluid migrates is one of the important factors which control the types of chimney structures. Based on where the thermal fluid come from and geometrical characteristics of the chimney structures, this paper classified the genetic types of chimney structures, and concluded that there existed three types and six subtypes chimney structures: organic chimney structures generated by the hydrocarbon-bearing thermal fluid in middle-shallow layers, inorganic and commingling-genetic chimney structures generated by thermal fluid in middle-deep layers. According to the seismic profiles interpretations, well logging response analysis and mineralogical and petrological characteristics in the study area, the author summarized the comprehensive identification marks for chimney structures. Especially the horizon velocity analysis method that is established in this paper and takes advantage of interval velocity anomaly is a semi-quantitative and reliable method of chimney structure s identification. It was pointed out in this paper that the occurrence of the chimney structures in the Taibei depression made the mechanism of accumulation complicated. The author provided proof of episodic accumulation of hydrocarbon in this area: The organic component in the boiling inclusion is the trail of petroleum migration, showing the causality between the boiling of thermal fluid and the chimney structures, meanwhile showing the paroxysmal accumulation is an important petroleum accumulation model. Based on the evolutionary characteristics of various types of chimney structures, this paper discussed their relationships with the migration-accumulation of petroleum respectively. At the same time, the author summarized the accumulating-dynamical models associated with chimney structures. The author analyzed such accumulation mechanisms as the facies state, direction, power of petroleum migration, the conditions of trap, the accumulation, leakage and reservation of petroleum, and the distribution rule of petroleum. The author also provides explanation for such practical problems the existence of a lot of mantle-derived CO_2, and its heterogeneous distribution on plane. By study on and recognition for chimney structure, the existence and distribution of much mantle-derived CO_2 found in this area are explained. Caused by tectonic thermal activities, the deep magma with much CO_2-bearing thermal fluid migrate upward along deep fault and chimney structures, which makes two wells within relatively short distance different gas composition, such as in well LF-1 and well LS36-1-1. Meanwhile, the author predicted the distribution of petroleum accumulation belt in middle-shallow layer for this area, pointed out the three favorable exploration areas in future, and provided the scientific and deciding references for future study on the commingling-genetic accumulation of petroleum in middle-deep layer and the new energy-gas hydrate.

DAS24-3B数据采集系统控制系统软件的研制与开发

Under the auspices of the 'knowledge-Innovation Program' of CAS, Institute of Geology and Geophysics has established the Broadband Seismic Laboratory. A new kind of 24-bit high-resolution seismograph DAS24-3B has been designed and manufactured in an effort of developing China's own technology of seismic array. Since these instruments will primarily be used in field operation, there is a need to optimize the system software of data acquisition system (DAS) to enhance its stability, compatibility and maintenance. The design ideas of the system software of DAS24-3B are partly learned from the advanced DAS 72A-08. In this system there are two exclusive communication programs DNAPI-COM1 and DNAPI-LPT1, which are suitable for all standard industrial computers with ECP parallel port and serial port. By these exclusive parallel and serial communication interface the system software is split into three parts, acquisition program, user's control program and graphical display program, which can function well in separate units and can run correctly in whole. The three parts of DAS24-3B's system software possess different functions and advantages. The function of acquisition program is to control the process of seismic data acquisition. DAS24-3B system reduced its power and harddisk read-write disturbance by using the extended memory attached to its CPU, which functions as enlarging the data buffer of system and lessening the times of harddisk read-write operations. Since GPS receiver of DAS is strongly sensitive to the around environment and has the possibility of signal loss the acquisition program has been designed with the ability to automatically trail the GPS locked time. The function of user's controlling program is to configure the system's work environment, to inform the user's commands to DAS, to trail the status of DAS in real-time. The function of graphical display program is to illustrate data in figures, to convert data file into some common formatted file, to split data file in parts and combine data files into one. Both user's control program and graphical display program are API (Application Programming Interface) in window 95/98 system. Both possess the features of clearness and friendship by use of all kind of window controls, which are composed by menu, toolbar, statusbar, dialogue box, message box, edit box, scrollbar, time control, button and so on. Two programs of systemic exception handles are provided to treat the trouble in field. The DAS24-3B DAS has been designed to be easier to use-better ability, more stable and simpler. It has been tested in field and base station and has been proved more suitable for field operation of seismic array than other native instruments.

维持性血液透析患者认知功能特点研究

Objective: To study the episodic memory, semantic memory, cognitive planning ability and inhibition ability in MHD patients. Method: Neuropsychological research methods such as Action memory of verb-object phrase, Trail Making Test (A and B), Verbal Fluency Test, Go-No/Go test and Stroop Color Naming Task were used to investigate Episodic Memory 、Semantic Memory、Executive Function of 40 MHD and 40 NC. Self-Rating Anxiety Scale (SAS), Self-Rating Depression Scale(SDS), Social Support Scale, Life Satisfaction Scale, and biochemical examination were applied and their relationships with cognitive function were analized. The mean age and education level of MHD group and NC group have no significant difference. Result: 1.Action memory of verb-object phrase differed significantly between MHD group and NC group. 2.Two tests of Verbal Fluency differed significantly between MHD group and NC group. 3.Trail Making Test A, Trail Making Test B, the baseline condition of Go-No/Go Test and Stroop Color Naming Test differed significantly between MHD group and NC group. 4.There is no significant difference between MHD group and NC group on the correct rate of No/Go Test and the baseline condition. Both groups showed Stroop Effect in Go-No/Go test, but MHD group performed significantly worse. 5.In Stroop Color Naming Task Test, NC group showed Stroop Effect, significant Repeated Distraction Promotion Effect and significant Negative Priming Effect,while MHD group showed only Stroop Effect and no Repeated Distraction Promotion Effect and no Negative Priming Effect. There is significant difference in Stroop Effect between MHD group and NC group. Conclusion: 1.Comparing with NC group, episodic memory, semantic memory, cognitive planning ability, and inhibition ability of MHD group were impaired significantly. 2.The pathological aging of Executive Function in MHD group showed: executive Function should be a unitary system. 3.Cognitive impairment is negatively correlated with serum creatinine, blood pressure and anxiety score in MHD patients; and is related with hemoglobin, hematocrit, social support and life satisfaction. Keyword: maintenance hemodialysis, episodic memory, semantic memory, cognitive planning, inhibition ability.

2型糖尿病患者认知功能特点及相关影响因素研究

Objective: Type 2 diabetes patients’ performances of action memory , semantic memory and working memory and the related factors were explored. Methods: 60 Type 2 diabetes patients were compared with 60 age and gender and level of education matched non-diabetes controls. Mood were tested by SAS and SDS, MMSE was used to test the basic cognitive function, Trail Making Test A and B, Verbal fluency test, Go-No/Go test, and Stroop color-word test were used to investigate the executive function of Type 2 diabetes patients and normal controls (NC). Patients’ GLU, TG, TCH, HbA1c, insulin and Cp were tested and correlated with their action memory and working memory. Results: There was no difference between NC group and Type 2 diabetes patients in MMSE scores. There is depression and anxiety mood in Type 2 diabetes patients. Type 2 diabetes patients get lower score in action memory test. Comparing to NC group, Type 2 diabetes patients performed significantly worse in Trail Making Test A and B and verbal fluency test. In Stroop Test, NC group showed significant Stroop Effect and Repeated Distraction Promotion Effect and Negative Priming Effect. However, In Type 2 diabetes group, only the Stroop Effect appeared, but no Repeated Distraction Promotion Effect and Negative Priming Effect. There is no difference between Type 2 diabetes and NC in Stroop Effect. In Go-No/Go test, both of two groups showed significant Stroop Effect, however, there was no difference between them. And also there is no difference on error rate of all levels between them. The course of disease, GL, HbA1c, TG, TCH, INS and Cp affected action memory and working memory. Conclusion: Type 2 diabetes patients’ action memory, semantic memory and working memory were partially impaired. Controlling the levels of GLU, TG and TCH can delay these kinds of impairment.