96 resultados para död


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摘要 "基于形态-地理学方法,通过野外调查,结合大量标本研究,在前人研究的基础上,对海南蕨类植物的分类进行了进一步修订;主要根据蕨类植物的现代地理分布,结合古生物学等有关资料,初步探讨了海南蕨类植物的区系性质与起源;根据IUCN2001年红色名录的等级和标准,对海南濒危蕨类植物的现状进行了初步评估,讨论了海南蕨类植物的受威胁原因,提出了有关保护的对策。主要结果如下: 1. 海南现有蕨类植物56科140属439个种及种下分类群(包括421种、15变种、2亚种和1变型),其中包括1个中国分布新记录和27个海南分布新记录,1新种――海南符藤蕨Teratophyllum hainanense;另有11个名称首次被处理为异名;澄清了海南假瘤蕨Phymatopteris hainanensis和圆顶假瘤蕨P. obtusa的模式问题,为滇桂三相蕨Ataxipteris dianguiensis、海南假瘤蕨P. hainanensis和浅杯鳞盖蕨Microlepia ampla指定了后选模式。 2. 海南蕨类区系具有以下特点:i. 以水龙骨科Polypodiaceae、金星蕨科Thelypteridaceae、铁角蕨科Aspleniaceae、叉蕨科Tectariaceae和观音座莲科Angiopteridaceae为表征科;ii. 明显的热带性质,科的97.5%、属的92.5%、种的83.6%为热带分布成分;iii. 很高的物种多样性与物种密度,但属内种系贫乏;iv. 与中南半岛的联系最为紧密,海南140个蕨类属中有136个与中南半岛共有,两地属的相似性系数达到87.2%;v. 海南蕨类区系就地起源于华夏古陆,起源时间可以追溯至早石炭世以前。 3. 海南439种蕨类(包括421种、15变种、2亚种和1变型)中,183种为常见蕨类,113种属于资料缺乏的种类(DD),47种属于近危(NT),53种属于易危(VU),37种属于濒危(EN),6种属于极危(CR)。海南的受威胁蕨类植物有96种,海南的绝大部分受威胁蕨类植物都生于保护区以内或得到有效保护的林区之内,已初步得到保护。导致海南96种蕨类受威胁的因素,除了植物本身的生物生态学特性和地理分布上的限制外,主要是人类活动的影响,特别是海南森林在上个世纪被大规模砍伐。为了保护这些受威胁植物,应加强保护区和林区的管理,实施就地保护,积极开展迁地保护和人工繁殖。 "

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臭氧属于二次污染物,它是由机动车、工厂等人为源以及天然源排放的氮氧化物(NOx)和挥发性有机物(VOCs)等一次污染物在大气中经过光化学反应形成的。O3 是光化学烟雾的主要成分,可对植物生长产生抑制。近几十年来,全球O3 污染的格局正在发生着巨大改变。由于北美及西欧等经济发达地区采取了有效控制臭氧形成前体物的措施,其空气中的O3 浓度在减少,而亚洲等经济发展中地区的O3 形成前体物的排放却在急剧攀升,导致大气中O3 浓度显著增加。中国经济的快速发展以及汽车保有量的迅猛增加导致O3 前体物的大量排放,许多经济较发达的地区空气中的O3 浓度超过了75ppb。由于O3 污染将导致农作物产量显著降低,因此,亚洲尤其是中国O3 污染对本地区农业生产的影响引起了国内外科学家的广泛关注。然而,在中国开展的关于O3 对植物生长及生产影响的研究相对较少,但已有的几篇研究报道确实指出目前中国部分地区的O3 浓度可导致冬小麦产量大幅下降,并预测到2020 年由O3 污染将引起小麦产量进一步降低。 植物对臭氧的反应或敏感性取决于诸如叶片导度、叶片结构及生化解毒等很多方面。首先,由于高叶片导度将吸收较多的臭氧量,因此,叶片导度通常被认为是决定抗性最为重要的因子。处于湿润条件下的植物,通常具有较高叶片导度,受到臭氧危害的程度一般也较大。其次,植物抗氧化胁迫能力的大小也决定着其对臭氧的敏感性。同一植株的老叶首先表现出伤害症状,这是由于老叶的抗氧化能力差于新叶,体现在抗坏血酸和谷胱甘肽含量及抗坏血酸氧化物酶和谷胱甘肽还原酶活性低于新叶。另外,叶片对臭氧的敏感程度与其叶片结构关系密切,拥有较大的细胞间隙对抗污染特性至关重要,由于叶片上表面的栅栏组织较海绵组织致密,因此通常较早表现出伤害症状。 影响植物对臭氧反应的环境因子很多,诸如光照、水气压亏、温度等。由于臭氧主要通过气孔进入植物体内,因此目前的研究主要集中在能显著调节气孔导度的环境因子,如土壤水分状况和在未来可能会与大气中臭氧浓度同步增加的CO2 浓度。CO2 浓度升高可降低植物的气孔导度,因此,CO2 浓度升高可减少叶片对O3 的吸收量。同时,大气CO2 浓度升高可提高净同化速率,可导致气孔的部分关闭而减少蒸腾,从而显著提高植株的水分利用效率,最终促进作物生长并提高产量。然而,二者对作物产量的交互影响尚不明确。水分胁迫被认为是影响O3 对植株伤害的一个重要环境因子。与正常供水相比,水分胁迫常常伴随着气孔导度的降低,导致进入到植株体内的O3 量相对较少而减轻植株受到的伤害程度。然而水分供应不足本身将导致小麦生长降低及产量下降。因此,水分亏缺可能会保护植株免受O3 伤害,同时也可能会加剧对植株的胁迫。 高浓度臭氧环境下,植物表现出较低的气孔导度。但研究表明,对臭氧敏感性不同的植物其气孔导度对臭氧的反应程度不同。臭氧对气孔的作用将影响植物生产力,同时也将影响植物对其它环境胁迫如干旱等的反应。短时间臭氧熏蒸小麦导致叶片细胞膜系统受损、光合产物输出受阻;而长期受臭氧污染后,小麦叶片的光合速率、光化学效率、叶绿素含量和蔗糖含量均显著降低,并与臭氧剂量的大小和峰值出现的早晚有关。O3 浓度升高将抑制光合作用,减少气孔导度,加强呼吸作用,改变C 同化物分配,加快叶片的衰老。众多研究表明,O3 导致的光合能力下降主要是由Rubisco 最大羧化效率降低导致;而O3 对光合器官捕获光的能力及光合电子传递速率的影响是光合作用下降的另一个原因。 尽管已有不少关于不同物种间对O3 敏感性的种间差异研究,然而育种方法或育种地点对中国不同冬小麦品种的O3 敏感性的影响尚不清楚。因此,我们假设育种年代、育种方法及地点将交互影响冬小麦品种对O3 的生长及生理响应。为进一步明确基因对冬小麦O3 敏感性的控制,研究了普通六倍体冬小麦的近缘体对O3 敏感性的差异。CO2 浓度升高及干旱胁迫对小麦臭氧敏感性的影响也进行了研究。论文主要从生理生化、生长及产量水平上来阐释O3 浓度升高、CO3加倍、干旱对冬小麦生长及生产影响的机理。 本研究主要是在温室中的上部开口的生长箱(open-top chamber, OTC)中进行。先后开展了四个盆栽实验研究,主要目的是确定中国不同基因型冬小麦种或品种对臭氧的敏感性及其反应机理;确定CO2 浓度升高及干旱在减轻O3 伤害方面的作用及其机理。实验材料为中国不同年代选育出的小麦品种,即1745年至2004 年间选育出的20 个品种和7 个小麦材料。主要评价指标包括相对生长速率、异速生长系数、叶绿素荧光、抗氧化活性、可溶性蛋白质含量、膜酯过氧化、气体交换、光合能力、叶绿素含量、暗呼吸、生物量及籽粒产量。实验研究得到的主要结果如下: 1) O3 升高显著降低整株及地上和地下部分的相对生长速率,显著降低异速生长系数、可变荧光、最大光化学效率、量子产额、光化学淬灭系数以及电子传递速率,但提高了非光化学淬灭系数。冬小麦不同品种对O3 的敏感性随育种年代的增加而增大,并与对照植株相对生长速率呈正相关。尽管近年来环境中的O3 浓度比过去显著增加,但新近育出的品种对臭氧的抗性却没有表现出协同进化效应。通过杂交选育的品种对臭氧的敏感性大于通过引进的和重选的品种。从生长和光合生理上来看,不同小麦品种对臭氧的敏感性与育种地点没有相关性,表明冬小麦品种对臭氧的适应能力与其生长环境下的臭氧浓度无关。因此,对臭氧相对敏感的冬小麦品种主要是由培育中较高相对生长速率或较高光合能力的杂交育种方式决定的,而与选育地点环境中的臭氧浓度无关。 2) 臭氧显著降低叶片中抗坏血酸(AsA)和可溶性蛋白的含量,但提高了过氧化物酶(POD)的活性和膜酯过氧化物(MDA)的含量。臭氧浓度升高抑制饱和光强下的净光合速率(Asat),降低气孔导度(gs)和总叶绿素含量,而显著提高暗呼吸速率(Rd)和胞间CO2 浓度(Ci)。臭氧导致总生物量降低,但地下部生物量受到的影响大于地上部。不同基因型小麦对臭氧的潜在敏感性与实际观察到的抗臭氧能力存在很大差异。冬小麦品种对臭氧的敏感性与臭氧环境下植株气孔导度和暗呼吸速率相关。臭氧导致Ci 浓度升高以及膜酯过氧化,由此得出臭氧导致的净光合速率主要是由于臭氧降低了叶肉细胞活性及细胞膜的完整性。新品种对臭氧相对敏感,主要是由于其具有较高的气孔导度抗氧化能力下降幅度较大以及较低的暗呼吸速率,从而对蛋白和细胞膜完整性造成较高的氧化伤害。 3) 臭氧对冬小麦光合和生长的影响存在着显著的种间差异。原初栽培种表现出最大的抗性,当代品种次之,而野生种对臭氧最为敏感。在普通冬小麦不同基因组供体中,钩刺山羊草(Aegilops tauschii,DD)对臭氧最敏感,其次为栽培一粒小麦(T. monococcum,AA),而圆锥小麦(Triticum turgidum ssp.Durum,AABB)对臭氧的抗性最大。因此,当代冬小麦品种对臭氧的敏感性可能是与其D 染色体供体-钩刺山羊草对臭氧敏感有关,而与其A、B 染色体供体-圆锥小麦的关系相对较小。 4) CO2 浓度升高提高了老品种和新品种的Asat,最大羧化速率(Vcmax),最大电子传递速率(Jmax)、光和CO2 饱和光合速率(Amax)。与之相反,臭氧显著降低了这些生理参数。虽然两品种对CO2 的响应没有显著性差异,但CO2浓度升高均有效保护了臭氧对它们的伤害。这种效应与CO2 浓度升高引起的气孔导度降低无关,而与代谢活性的提高有关。 5) 水分胁迫和臭氧分别都显著降低了 Asat 和gs。干旱显著降低Vcmax 和羧化效率(CE),而对Jmax 和暗呼吸(R)的影响不显著。臭氧显著降低冬小麦不同基因型的Vcmax,Jmax,R 和CE。二者均降低了生物量的积累及最终籽粒产量。与六倍体小麦相比,四倍体小麦对干旱相对敏感,但对臭氧却表现出较高抗性。干旱降低了气孔导度从而显著减少了植株对臭氧的吸收量,但两基因型的反应截然不同。干旱使臭氧对六倍体小麦产量和收获指数的伤害分别减少了约16%和50%,而干旱对该四倍体小麦的保护效应不大。

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保护生物地理学(Conservation biogeography)就是运用生物地理学的原理、理论和分析方法,特别是那些有关物种分布格局的信息,去解决生物多样性保护的相关问题,是当前生物多样性及其保护生物学研究领域的热点。通过构建中国蕨类植物物种及分布数据库得知,中国现有蕨类植物63科,221属,2456种,其中有1218种属于中国特有(中国蕨类总数约50%),3种蕨类植物为外来种。科的统计表明:最大的科为鳞毛蕨科、蹄盖蕨科、金星蕨科和水龙骨科4个科所包含的种类占全国总种数的51.5%。鳞毛蕨科、蹄盖蕨科、金星蕨科、水龙骨科、铁角蕨科、凤尾蕨科、碗蕨科、中国蕨科、叉蕨科、观音座莲科等十个大科所含种数占了中国特有种总数的80%。云南、四川、贵州、广西、台湾、湖南、西藏等省区是中国蕨类植物种类数量及中国特有种数量较多的地区;全国有22个省区拥有地方特有种,地方特有蕨类植物数量由西向东、由南向北逐渐递减,云南、台湾、西藏、海南等四省区的地方特有蕨类植物种数占各省区蕨类植物总数10%以上,反映了青藏高原隆起及岛屿现象对中国特有蕨类植物分化的影响。 中国蕨类植物与各地理、环境及气候因子的相关性分析结果表明:(1)随着经度、纬度的增加, 蕨类植物物种总数及中国特有蕨类植物总数具有减少的趋势;(2)蕨类植物在海拔1000-1600米之间、特有蕨类植物在海拔700-2100米之间具有最大的多样性,此区间外随着海拔的降低及升高,物种丰富度依次降低;(3)气候因子特别是一月均温、年均温、相对湿度、年降雨量等因素对蕨类植物及中国特有蕨类植物均具有较大的影响,与最热月均温成低度线性相关或相关不显著;(4)地形因子是影响蕨类植物多样性的一个独立因素,地形条件越复杂,蕨类植物种类及中国特有蕨类植物种类越丰富。 运用SPSS统计分析软件分析了中国蕨类植物在各省之间种的相似性,以省区为单元对中国各省区蕨类植物物种组成进行了聚类分析,最后结果划分成:①南方蕨类植物区(包括横断山—青藏高原亚区、华中亚区、华东亚区、华南亚区)和北方蕨类植物区(包括东北—华北—西北亚区、秦岭亚区、江淮亚区)。 中国蕨类植物区系地理成分分析结果表明:(1)中国—喜马拉雅分布(40.86%)、热带亚洲分布(30.95%)、东亚分布(12.77%)和中国—日本分布(8.24%)构成中国蕨类植物区系成分的主体;在洲际关系方面,中国蕨类植物与大洋洲的蕨类植物关系最为紧密,共有种最多,达94种;与南美洲共有的蕨类植物种类最少,仅32种。(2)基于地理成分的聚类分析结果表明,可将我国蕨类植物区系地理区划为代表温带亚洲成分的(特别是中亚、西亚地中海分布成分)西北温带亚洲植物区、代表古热带成分的华南古热带植物区和代表广义东亚成分的东亚植物区,东亚植物区可继续下分为中国东北—俄罗斯远东亚区(该区分布有大量的东亚-北美间断分布类型)、中国—日本植物亚区、中国—喜马拉雅植物亚区。 运用IUCN濒危物种等级对中国蕨类植物及其国家重点保护蕨类植物进行了现状评估,结果表明:可能灭绝(EX)的2种,极危(CR)的蕨类植物有33种,属于濒危(EN)的蕨类植物51种,属于易危(VU)的蕨类植物有109种,接近受危(NT)的蕨类植物158种,需予关注(LC)的845种,数据不足(DD)的1255种。对国家重点保护蕨类植物的保护级别提出部分变更的建议,对部分没有列入保护名录珍稀蕨类提出保护的建议,并就建议保护级别变更和增加保护的种类进行了必要的说明。

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Background: Hair is unique to mammals. Keratin associated proteins (KRTAPs), which contain two major groups: high/ultrahigh cysteine and high glycine-tyrosine, are one of the major components of hair and play essential roles in the formation of rigid and

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This paper reviews a large number of genes under positive Darwinian selection in modern human populations, such as brain development genes, immunity genes, reproductive related genes, perception receptors. The research on the evolutionary property of thes

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In addition to its medical importance as parasitic pathogen, Entamoeba has aroused people's interest in its evolutionary status for a long time. Lacking mitochondrion and other intracellular organelles common to typical eukaryotes, Entamoeba and several other amitochondrial protozoans have been recognized as ancient pre-mitochondriate eukaryotes and named "archezoa", the most primitive extant eukaryotes. It was suggested that they might be living fossils that remained in a primitive stage of evolution before acquisition of organelles, lying close to the transition between prokaryotes and eukaryotes. However, recent studies revealed that Entamoeba contained an organelle, "crypton" or "mitosome", which was regarded as specialized or reductive mitochondrion. Relative molecular phylogenetic analyses also indicated the existence or the probable existence of mitochondrion in Entamoeba. Our phylogenetic analysis based on DNA topoisomerase II strongly suggested its divergence after some mitchondriate eukaryotes. Here, all these recent researches are reviewed and the evolutionary status of Entamoeba is discussed.

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Several groups of parasitic protozoa, as represented by Giardia, Trichomonas, Entamoeba and Microsporida, were once widely considered to be the most primitive extant eukaryotic group - Archezoa. The main evidence for this is their 'lacking mitochondria' and possessing some other primitive features between prokaryotes and eukaryotes, and being basal to all eukaryotes with mitochondria in phylogenies inferred from many molecules. Some authors even proposed that these organisms diverged before the endosymbiotic origin of mitochondria within eukaryotes. This view was once considered to be very significant to the study of origin and evolution of eukaryotic cells (eukaryotes). However, in recent years this has been challenged by accumulating evidence from new studies. Here the sequences of DNA topoisomerase 11 in G lamblia, T vaginalis and E histolytica were identified first by PCR and sequencing, then combining with the sequence data of the microsporidia Encephalitozoon cunicul and other eukaryotic groups of different evolutionary positions from GenBank, phylogenetic trees were constructed by various methods to investigate the evolutionary positions of these amitochondriate protozoa. Our results showed that since the characteristics of DNA topoisomerase 11 make it avoid the defect of 'long-branch attraction' appearing in the previous phylogenetic analyses, our trees can not only reflect effectively the relationship of different major eukaryotic groups, which is widely accepted, but also reveal phylogenetic positions for these amitochondriate protozoa, which is different from the previous phylogenetic trees. They are not the earliest-branching eukaryotes, but diverged after some mitochondriate organisms such as kinetoplastids and mycetozoan; they are not a united group but occupy different phylogenetic positions. Combining with the recent cytological findings of mitochondria-like organelles in them, we think that though some of them (e.g. diplomonads, as represented by Giardia) may occupy a very low evolutionary position, generally these organisms are not as extremely primitive as was thought before; they should be polyphyletic groups diverging after the endosymbiotic origin of mitochondrion to adapt themselves to anaerobic parasitic life.

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The genes encoding type II DNA topoisomerases were investigated in Giardia lamblia genome, and a type IIA gene, GlTop 2 was identified. It is a single copy gene with a 4476 by long ORF without intron. The deduced amino acid sequence shows strong homology to eukaryotic DNA Top 2. However, some distortions were found, such as six insertions in the ATPase domain and the central domain, a similar to 100 as longer central domain; a similar to 200 as shorter C-terminal domain containing rich charged residues. These features revealed by comparing with Top 2 of the host, human, might be helpful in exploiting drug selectivity for antigiardial therapy. Phylogenetic analysis of eukaryotic enzymes showed that kinetoplastids, plants, fungi, and animals were monophyletic groups, and the animal and fungi lineages shared a more recent common ancestor than either did with the plant lineage; microsporidia grouped with fungi. However, unlike many previous phylogenetic analyses, the "amitochondriate" G. lamblia was not the earliest branch but diverged after mitochondriate kinetoplastids in our trees. Both the finding of typical eukaryotic type IIA topoisomerase and the phylogenetic analysis suggest G. lamblia is not possibly as primitive as was regarded before and might diverge after the acquisition of mitochondria. This is consistent with the recent discovery of mitochondrial remnant organelles in G. lamblia.

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Giaridia lamblia was long considered to be one of the most primitive eukaryotes and to lie close to the transition between prokaryotes and eukaryotes, but several supporting features, such as lack of mitochondrion and Golgi, have been challenged recently. It was also reported previously that G. lamblia lacked nucleolus, which is the site of pre-rRNA processing and ribosomal assembling in the other eukaryotic cells. Here, we report the identification of the yeast homolog gene, krr1, in the anucleolate eukaryote, G. lamblia. The krr1 gene, encoding one of the pre-rRNA processing proteins in yeast, is actively transcribed in G. lamblia. The deduced protein sequence of G. lamblia krr1 is highly similar to yeast KRR1p that contains a single-KH domain. Our database searches indicated that krr1 genes actually present in diverse eukaryotes and also seem to present in Archaea. However, only the eukaryotic homologs, including that of G. lamblia, have the single-KH domain, which contains the conserved motif KR(K)R. Fibrillarin, another important pre-rRNA processing protein has also been identified previously in G. lamblia. Moreover, our database search shows that nearly half of the other nucleolus-localized protein genes of eukaryotic cells also have their homologs in Giardia. Therefore, we suggest that a common mechanism of pre-RNA processing may operate in the anucleolate eukaryote G. lamblia and in the other eukaryotes and that like the case of "lack of mitochondrion," "lack of nucleolus" may not be a primitive feature, but a secondarily evolutionary condition of the parasite.

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以量子化学PM3算法计算得到的 12个量子化学参数作为分子结构描述符 ,运用偏最小二乘分析 ,首次建立 2 3种PC DD F水溶解度 (logSW)的定量结构 性质关系模型 .PCDD F的SW 随着其分子量的增大而减小 ;随着其分子最低未占据轨道能 (Elumo)的增大而增大 ,推测PCDD F分子与水分子之间存在着电荷转移相互作用

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Gibel carp (Carassius auratus gibelio Bloch) is a natural gynogenetic fish which requires sperm of the same or related species to activate egg development. The eggs of one gibel carp were divided into two batches. One batch was 'fertilized' with sperm from gibel carp (strain DD), and the other 'fertilized' with sperm from red common carp (Cyprinus carpio red variety) (strain DR). The juveniles were transferred to the laboratory 36 days post-hatch. Triplicate groups of each strain were fed a formulated diet at either 3% or satiation ration for 8 weeks. At both the restricted and satiation rations, specific growth rate was significantly higher in strain DR than in strain DD. At the 3% ration, there was no significant difference in feeding rate or feed conversion efficiency between the two strains. At the satiation ration, strain DR had a significantly lower feeding rate but higher feed conversion efficiency than strain DD. At the satiation ration, strain DR had a significantly lower intake protein, but higher recovered protein than strain DD. There was no significant difference in faecal protein loss between the two strains. At the 3% ration, strain had no significant effects on intake protein, faecal protein or recovered protein. Neither faecal energy loss nor recovered energy was affected by strain or ration. At both the 3% and satiation ration, final body contents of dry matter and lipid were significantly lower in strain DR than strain DD, while there was no significant difference in protein and energy content between the two strains at either ration level. The results suggested that gibel carp 'fertilized' with sperm of common carp grew faster than those 'fertilized' with sperm of gibel carp through increased feed conversion efficiency and protein retention.

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N-p-n Si/SiGe/Si heterostructures have been grown by a disilane (Si2H6) gas and Ge solid sources molecular beam epitaxy system using phosphine (PH3) and diborane (B2H6) as n- and p-type in situ doping sources, respectively. Adopting an in situ doping control technology, the influence of background B dopant on the growth of n-Si emitter layer was reduced, and an abrupt B dopant distribution from SiGe base to Si emitter layer was obtained. Besides, higher n-type doping in the surface region of emitter to reduce the emitter resist can be realized, and it did not result in the drop of growth rate of Si emitter layer in this technology. (C) 2004 Elsevier B.V. All rights reserved.

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Self-assembled Ge islands were grown on Si(100) substrate by Si2H6-Ge molecular beam epitaxy. After being subjected to chemical etching, it is found that the photoluminescence from the etched Ge islands became more intense and shifted to the higher-energy side compared to that of the as-deposited Ge islands. This behaviour was explained by the effect of chemical etching on the morphology of the Ge islands. Our results demonstrate that chemical etching can be a way to change the luminescence property of the as-deposited islands.

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Self-assembled Ge islands were grown on Si (1 0 0) substrate by Si2H6-Ge molecular beam epitaxy. Subjected to a chemical etching, it is found that the size and shape (i.e. ratio of height to base width) of Ge islands change with etching time. In addition, the photoluminescence from the etched Ge islands shifted to the higher energy side compared to that of the as-deposited Ge islands. Our results demonstrated that chemical etching can be a way to change the size and shape of the as-deposited islands as well as their luminescence property. (C) 2001 Elsevier Science B.V. All rights reserved.

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The effects of annealing time and Si cap layer thickness: on the thermal stability of the Si/SiGe/Si heterostructures deposited by disilane and solid-Ge molecule beam epitaxy were investigated. It is found that in the same strain state of the SiGe layers the annealing time decreases with increasing Si cap layer thickness. This effect is analyzed by a force-balance theory and an equation has been obtained to characterize the relation between the annealing time and the Si cap layer thickness. (C) 2001 Elsevier Science B.V. All rights reserved.