利用3对引物鉴定产毒和非产毒微囊藻

根据微囊藻毒素合成酶基因簇序列 ,合成了 3对引物epF/mb1R ,mcF/teR ,mcF/umR ,通过全细胞PCR的方法检测了 19种不同来源微囊藻产毒的情况。 3种引物对 15株产毒微囊藻中均可扩增到预期大小的片段 ,测序结果证明这些片段是微囊藻毒素合成酶基因片段。PCR反应结果与HPLC分析所得到的结果有良好的对应性。在此基础上 ,初步确定了 3对引物检测产毒微囊藻对细胞浓度要求的下限。与其它引物相比 ,3对引物的特异性强 ,扩增条带大小适中 ,便于观察

Establishment and characterization of dual-species biofilms formed between a 3,5-dinitrobenzoic-degrading strain and bacteria with high biofilm-forming capabilities

In this study, the possibility of establishing a dual-species biofilm from a bacterium with a high biofilm-forming capability and a 3,5-dinitrobenzoic acid (3,5-DNBA)-degrading bacterium, Comamonas testosteroni A3, was investigated. Our results showed that the combinations of strain A3 with each of five strains with a high biofilm-forming capability (Pseudomonas sp. M8, Pseudomonas putida M9, Bacillus cereus M19, Pseudomonas plecoglossicida M21 and Aeromonas hydrophila M22) presented different levels of enhancement regarding biofilm-forming capability. Among these culture combinations, the 24-h dual-species biofilms established by C. testosteroni A3 with P. putida M9 and A. hydrophila M22 showed the strongest resistance to 3,5-DNBA shock loading, as demonstrated by six successive replacements with DMM2 synthetic wastewater. The degradation rates of 3,5-DNBA by these two culture combinations reached 63.3-91.6% and 70.7-89.4%, respectively, within 6 h of every replacement. Using the gfp-tagged strain M22 and confocal laser scanning microscopy, the immobilization of A3 cells in the dual-species biofilm was confirmed. We thus demonstrated that, during wastewater treatment processes, it is possible to immobilize degrader bacteria with bacteria with a high biofilm-forming capability and to enable them to develop into the mixed microbial flora. This may be a simple and economical method that represents a novel strategy for effective bioaugmentation.

N-(间硝基苯磺酰)-6-甲基吲哚体外抗HIV 活性及作用机制研究

本论文对12个N-（取代苯磺酰）吲哚类化合物体外抗HIV活性进行了初步筛选，并选择其中活性最好的化合物N-（间硝基苯磺酰）-6-甲基吲哚，对其抗HIV 活性及作用机制进行深入研究。一、体外检测了12个N-（取代苯磺酰）吲哚类化合物对C8166和MT-4细胞的毒性、对HIV-1IIIB 感染C8166后诱导的合胞体形成的抑制及对HIV-1IIIB感染MT-4 细胞后的保护作用。结果发现多个化合物具有抑制HIV-1复制活性，特别是化合物N-（间硝基苯磺酰）-6-甲基吲哚，其对HIV-1IIIB诱导的合胞体形成的EC50和TI （Therapy index）值分别为0.26 μg/ml和543.78；对HIV-1IIIB急性感染的MT-4细胞也有很好的保护作用（TI值为104.23)。二、选择活性最强的化合物N-（间硝基苯磺酰）-6-甲基吲哚进行深入的抗 HIV活性研究。在细胞水平上，通过观察致感染细胞病变和HIV-1 p24抗原表达抑制实验（ELISA方法），采用3类多株HIV病毒株（实验株、临床分离株、耐药株）和3类多种细胞（人T淋巴细胞传代株、HIV-1慢性感染人T淋巴细胞株、人外周血单个核细胞）对化合物进行体外抗HIV活性进行系统评价，实验结果表明，化合物对不同来源的HIV-1病毒株都显示出很好抗HIV活性。同时，我们也研究了化合物抗HIV-2活性，发现该化合物并不能抑制HIV-2在C8166细胞中复制。三、在细胞毒性实验上，我们检测了N-（间硝基苯磺酰）-6-甲基吲哚对不同的细胞系和人外周血单个核细胞（PBMC）毒性作用，结果显示化合物的细胞毒性较低。四、在作用机制和靶点研究上，检测了化合物对感染与未感染细胞之间融合的抑制、对HIV-1急性感染C8166细胞及对HIV-1慢性感染H9细胞（H9/HIV-1IIIB）中病毒复制的阻断作用。结果显示，N-（间硝基苯磺酰）-6-甲基吲哚对急性感染细胞有很好抑制作用（EC50为0.52 μg/ml）；但对感染与未感染细胞的融合（EC50为 46.40 μg/ml）和慢性感染H9细胞中病毒的复制没有抑制作用（EC50大于100 μg/ml）。提示化合物作用于HIV侵入细胞后到HIV DNA整合前这一阶段。其后对HIV-1逆转录酶活性的抑制情况进行了分析，发现N-（间硝基苯磺酰） -6-甲基吲哚对HIV-1逆转录酶（RT）有很好抑制作用。五、构效关系分析。我们对12 个N-（取代苯磺酰）吲哚类化合物进行了初步的构效关系研究，发现在N-benzenesulfonyl 环上连接一个吸电子基团(nitro group)比供电子基团(methyl group)有更强的抗HIV 活性，但当在indoles 环上连接吸电子基团(nitro group)，抗HIV 活性反而受到抑制。以上实验数据显示N-（间硝基苯磺酰）-6-甲基吲哚是一个安全有效的抗 HIV-1 候选化合物，具有进一步研究价值。

1-苯基-3-甲基-4-苯甲酰基-5-吡唑啉酮(PMBP)萃取钍流程的改进

在用PMBP(1 苯基 3 甲基 4 苯甲酰基 5 吡唑啉酮 )从 14MeV中子照射过的天然U靶溶液中萃取Th流程的基础上 ,将萃取介质改为HNO3溶液 ,并将单次萃取 反萃改为两次萃取 反萃 ;在反萃溶液中加入I- 载体和NaNO2溶液 ,在Fe(OH) 3沉淀溶液中加入Br- 载体。用改进后的流程从 6 0MeV/u18O离子轰击天然U的HNO3溶液中分离Th ,从制得的Th样品的γ射线谱可以看出 ,该流程能去除绝大部分产物元素 ,特别是能完全去除溴和碘

Synthesis of Arm-6 polystyrene by atom transfer radical polymerization

A new initiator for atom transfer radical polymerization (ATRP), (Cl-2 HCCOO)(3) -C-6 H-3, (TrDCAP),has been designed and successfully synthesized. ATRP of styrene was carried out by using TrDCAP as hexafunctional initiator and the CuCl/bpy catalyst at 130 degrees C in 30% THF via core-first strategy. The Arm-6 PS-AAP was synthesized by etherealization of Arm-6 PS and 4-(4'-methoxyphenylazomethine) phenol (AAP). The initiator and the architectures of the Arm-6 PS were confirmed by H-1-NMR, FT-IR, UV-Vis and GPC.

一种新的橙红色长余辉荧光材料Y_2O_2S∶Sm~(3+)

铜激活的硫化锌 (Zn S∶ Cu)和铕激活的硫化钙 (Ca S∶ Eu)是最早获得应用的蓝色和红色长余辉材料 .随后 ,相继发现了铝酸盐体系和硅酸盐体系两大类长余辉荧光材料 [1～ 3 ] .这两类长余辉荧光材料在发光亮度、余辉时间、稳定性方面都较前述硫化物系列长余辉荧光材料有很大提高 ,从而具有非常广阔的应用前景和应用范围 [4～ 6] .但这两类长余辉荧光材料的发光颜色一般为蓝紫、蓝或黄绿 ,没有红色发光现象 .随着研究的深入 ,人们发现了稀土元素激活的碱土钛酸盐红色长余辉荧光材料 ,这种荧光材料在发光亮度及余辉上都有明显的提高 [7,8] ,而且解决了硫化物不稳定的缺点 .近年来才发展起来的以碱土金属氧化物为发光基质 ,以 Eu3 + 为激活剂的红色长余辉荧光材料进一步提高了余辉亮度及时间 [9] .传统碱土硫化物类磷光体有较宽的激发谱带 ,可在不同的光源下激发 .但它的稳定性较差 ,必须进行工艺后处理才能得到应用 .其它几类磷光体的激发光源谱带较窄 ,仅限于紫外光源 ,因此使其应用范围受到限制 .目前已报道的各种长余辉红色荧光材料在余辉时间方面有较大的差异 ,从十几分钟到几百分钟不等 ,各类红色磷光...

Structure of coordination polymer, [Zn(bipy)(H2O)(3)SO4] center dot 2H(2)O (bipy=4,4 '-bipyridine)

The crystal of the title compound (C10H18N2O9SZn M-r=407.69) belongs to the hexagonal system, space group P 6(5) with cell parameters: a=11.411 (2), c=20.908(4) Angstrom, V=2357.7(7) Angstrom(3), Z=6, D-c=1.723g/cm(3), F(000)=1260, mu(MoKa)=1.743mm(-1). The final R and omega R factors are 0.072 and 0.178 respectively for 1335 observed reflections. in the structure, zinc ions are bridged by 4,4'-bipyridine to form infinite chains. The sheets containing parallel chains stack along a 65 screw axis to give a helical staircase motif. The helical structure is mainly controlled by the hydrogen bonds.

Synthesis, structure characterization and biological activity of a novel amino acid salt (HAla)(8)(H3O)(10)[PMo12O40](6) center dot 22H(2)O

A novel compound was synthesized and characterized by means of elemental analysis, IR and UV spectra, TG, CV and single crystal X-ray diffraction. The compound crystallized in an orthorhombic space group C222 with a=1. 622 4(3) nm, b=3. 498 4(7) nm, c=1. 301 5(3) nm, V=7. 387 (3) nm(3), Z=6, R-1= 0. 037 3, wR(2)=0. 114 0. The Ala (Ala = alanine) molecules were protonated at the amino nitrogen N (1) and the C (2) of Ala group with the terminal oxygen atom O(15), O(14), O(26) and O(27) of the polyoxometalates participating in the hydrogen bond network. The anti-tumor activity of the title compound was estimated against Hela and Pc-3m cancer cells.

Synthesis and characterization of a novel hybrid single crystal [Ga-3(PO4)(3)F-2]H3O center dot (H3NCH2CH2)(2)NH2

A novel three-dimensional fluorinated gallium phosphate has been hydrothermally,synthesized by using diethylenetriamine as an organic structure-directing agent. X-ray single crystal structure analysis indicates this compound crystallizes in the orthorhombic space group P-bca, a = 1. 605 6 (7) nm, b = 1.011 4 (4) nm, c=1. 854 6(5) nm, V=3. 011 6(19) nm(3), Z=4. The three-dimensional framework based on linkage of corner-sharing polyhedron PO4, GaO4F and GaO4F2 delimit ten-ring channels along b axis in which the triply protonated amines are located serving as charge compensating guests and supporters.

Synthesis and crystal structure of a novel compound: [H3PMo12O40][CO(NH2)(2)](3)center dot-5H(2)O

The title compound, [H3PMo12O40][CO(NH2)(2)](3). 5H(2)O, was synthesized and characterized by IR, C-13 NMR and X-ray diffraction. This is the first example of a urea-heteropoly acid species. Crystal data: monoclinic, C2/c, a = 17.790(4) Angstrom, b = 17.158(3) Angstrom, e = 25.512(5) Angstrom, beta = 100.65(3)degrees, V = 8514(3) Angstrom (3), Z = 6, R-1 = 0.0437, wR2 = 0. 1092. In the unit cell, the urea molecules occupy cavities in the polyoxometalate lattice ordered along b-axis. Water molecules occupy the space left by polyoxometalates, and urea. Polyoxometalate O atoms, the N atoms of urea and O atoms of water molecules are involved in hydrogen bonding. (C) 2001 Elsevier Science B.V. All rights reserved.

Hydrothermal synthesis and crystal structure of Co(En)(3)MoO4

Co(En)(3)MoO4 was synthesized by using the method of hydrothermal synthesis and characterized by elemental analysis, IR, ESR and single-crystal X-ray methods. It crystallizes in hexagonal space group P (3) over bar C1 with a=1.596 4(2) nm, b=1.596 4(2) nm, c = 0.993 5(2) nm, alpha=beta=90 degrees gamma=120 degrees, M-c=399.18, V=2.192 6(6) nm(3), D-c=1.814 g/nm(3), Z=6, F(000)=1 2181 R-1=0.070 3, R-w=0.220 7. According to separation of anion which acted on electrostatic potential, the anion and cation ions formed a type of organic and inorganic material.

TiO_2-Li_2O-Nb_2O_5∶Eu~(3+)体系的研究

本文研究了 TiO_2-Li_2O-Nb_2O_5:Eu~(3+)体系中固溶体形成的温度与组成的关系。化学通式为 Ti_(1-4x-y)EuLixNb_3xO_2,当 y=0.2,0