低剂量辐照下细胞辐射超敏感性研究

目的：1．比较肝癌细胞SMMC-7721和正常肝细胞L02，其辐射超敏感性和增强的辐射抗性的差异，为临床治疗提供基础研究数据。 2．对于重离子束辐照哺乳动物细胞引起的超敏感性以及增强的辐射抗性很少有报导。本研究利用兰州重离子研究装置（HIRFL）提供的12C开展高LET射线引起肝癌细胞SMMC-7721的辐射超敏感性的研究。材料和方法：1．低剂量60co γ射线辐照SMMc-7721细胞和L02细胞，低剂量高LE12c （50 MeV／u，LET＝44．56keV/μm）辐照SMMC-7721细胞，剂量范围为0-ZGy.2．利用流式细胞仪的前向光和侧向光对辐照后的细胞进行分选，精确计数。3．将细胞转入培养皿中，培养l0天，固定，染色。统计大于50个细胞的克隆。结果与讨论：1．用流式细胞仪进行细胞分选与传统的稀释法相比，存活分数没有明显的差异，标准偏差明显减少，该方法完全适用于低剂量存活的测量；2，SMMc-7721和L02细胞对低剂量60Coγ射线辐照均表现出HRS／IRR响应。但是只有当肿瘤细胞和正常组织的存活差异在3％以上时才能将HRS响应应用于临床治疗。3．用高LET12c离子束对SMMC-7721细胞进行照射，细胞也表现出明显的HRS／ IRR响应。与Y射线照射相比，C离子照射的闭值剂量为0．28Gy，比γ射线照射的要低。这可能是由于两种射线能量沉积的方式不同造成的。4．将重离子应用于临床治疗时，由于位于离子通道上的细胞受到微小剂量的照射（0．2-0.5SGy/次），细胞正好位于超敏感区域，因此，在治疗时要充分考虑离子束入射通道上细胞的HRS响应。

Anti-inflammatory and immunomodulatory activities of polysaccharide from Chlorella stigmatophora and Phaeodactylum tricornutum

Crude polysaccharide extracts were obtained from aqueous extracts of the microalgae Chlorella stigmatophora and Phaeodactylum tricornutum. The crude extracts were fractionated by ion-exchange chromatography on DEAE-cellulose columns. The molecular weights of the polysaccharides in each fraction were estimated by gel filtration on Sephacryl columns. The crude polysaccharide extracts of both microalgae showed anti-inflammatory activity in the carrageenan-induced paw edema test. In assays of effects on the delayed hyper-sensitivity response, and on phagocytic activity assayed in vivo and in vitro, the C. stigmatophora extract showed immunosuppressant effects, while the P. tricornutum extract showed immunostimulatory effects. Copyright © 2003 John Wiley & Sons, Ltd.

Effective dielectric responses of graded composites of the particles with a general power-law gradation profile

The effective dielectric response of graded spherical composites having general power-law gradient inclusions is investigated under a uniform applied electric field, where the dielectric gradation profile of the spherical inclusions is modeled by the equation epsilon(i) (r) = c(b+r)(k). Analytical solutions of the local electrical potentials are derived in terms of hyper-geometric function and the effective dielectric response of the graded composites is predicted in the dilute limit. From our result, the local potentials of graded spherical composites having both simple power-law dielectric profile epsilon(i)(r) = cr(k) and linear dielectric profile epsilon(i) (r) = c(b+r) are derived exactly by taking the limits b --> 0 and k --> 1, respectively. In the dilute limit, our exact result is used to test the validity of differential effective dipole approximation (DEDA) for estimating the effective response of graded spherical composites, and it is shown that the DEDA is in excellent agreement with exact result. (C) 2005 Elsevier B.V. All rights reserved.

Effective properties of graded elliptical cylindrical composites

The effective property has been investigated theoretically in graded elliptical cylindrical composite's consisting of inhomogeneous graded elliptical cylinders and an isotropic matrix under external uniform electric field. As a theoretical model, the dielectric gradient profile in the elliptical cylinder is modeled by a power-law function of short semi-axis variable parameter (xi(2) - 1) in the elliptical cylindrical coordinates, namely epsilon(i)(xi) = c(k) (xi(2) - 1)(k), where c(k) and k are the parameters, and xi is the long semi-axis space variable in an elliptical cylindrical inclusion region. In the dilute limit, the local analytical potentials in inclusion and matrix regions are derived exactly by means of the hyper-geometric function, and the formulas are given for estimating the effective dielectric responses under the external lfield along (x) over cap- and (y) over cap -directions, respectively. Furthermore, we have demonstrated that our effective response formulas can be reduced to the well-known results of homogeneous isotropic elliptical cylindrical composites if we take the limit k -> 0 in graded elliptical cylindrical composites. (c) 2006 Elsevier B.V. All rights reserved.

Effective ac dielectric response of graded cylindrical composites

Under an external alternating current (ac) field, the effective ac dielectric response of graded composites consisting of the graded cylindrical inclusion having complex permittivity profiles has been investigated theoretically. A model that the dielectric function is assumed to be a constant while the conductivity has a power-law dependence on the radial variable r, namely epsilon(i)(r) = A + cr(k)/i omega. is studied and the local analytical potentials of the inclusion and the host regions are derived in terms of hyper-geometric function. In the dilute limit, the effective ac dielectric response is predicted. Meanwhile, we have given the exact proof of the differential effective dipole approximation (DEDA) method, which is suitable to arbitrary graded profiles. Furthermore, we have given the analytical potentials and the effective ac dielectric responses of coated graded cylindrical composites for two cases, case (a) graded core and case (b) graded coated layer, having the graded dielectric profiles, respectively. (c) 2005 Elsevier B.V. All rights reserved.

超冗余度机械臂的路径安全性优化研究

提出了一个对超冗余度机械臂路径规划进行安全性优化的新方法 .采用一般随机路标法得到一个表示机械臂位形空间结构信息的路标 ,计算机械臂在各个位形下与障碍物之间的最小距离 ,并把此信息加到随机路标法所求的路标上 ,从而把路标转化为网格结构 .基于此网络 ,给出了一个对路径进行安全性优化的数学模型 ,利用其对超冗余度机械臂进行路径规划 .仿真结果表明 ,相对于一般随机路标法所建模型规划的路径安全性大大提高

基于超冗余度机械臂动力学的时间最优轨迹规划

基于超冗余度机械臂的动力学方程 ,提出了一种超冗余度机械臂同时受速度和力矩约束的时间最优轨迹规划方法 .它首先采用 B样条曲线拟合无碰撞离散路径 ,得到由伪位移参数 s表示的超冗余度机械臂连续、光滑运动路径 ,然后对动力学方程和约束方程进行数学变换 ,得到由 s表示的动力学方程和约束方程 ,最后以 s和伪速度 s· 分别作为动态规划的阶段变量和状态变量 ,对超冗余度机械臂进行时间最优轨迹规划 .仿真结果表明 ,所给出的时间最优轨迹规划算法是正确的 ,所采取的解决方法是可行的