Generation of recombinant monoclonal antibodies to study structure-function of envelope protein VP28 of white spot syndrome virus from shrimp

White spot syndrome virus (WSSV) is a major pathogen in shrimp aquaculture. VP28 is one of the most important envelope proteins of WSSV. In this study, a recombinant antibody library, as single-chain fragment variable (scFv) format, displayed on phage was constructed using mRNA from spleen cells of mice immunized with-full-length VP28 expressed in Escherichia coli. After several rounds of panning, six scFv antibodies specifically binding to the epitopes in the N-terminal, middle, and C-terminal regions of VP28, respectively, were isolated from the library. Using these scFv antibodies as tools, the epitopes in VP28 were located on the envelope of the virion by immuno-electron Microscopy, Neutralization assay with these antibodies in vitro suggested that these epitopes may not be the attachment site of WSSV to host cell receptor. This study provides a new way to investigate the structure and function of the envelope proteins of WSSV. (c) 2008 Published by Elsevier Inc.

A novel face recognition method based on hypersausage neural networks

In this paper, we firstly give the nature of 'hypersausages', study its structure and training of the network, then discuss the nature of it by way of experimenting with ORL face database, and finally, verify its unsurpassable advantages compared with other means.

Tunable optical properties of nano-Au on vanadium dioxide

The optical properties of Au nanoparticles deposited on thermochromic thin films of VO2 are investigated using spectroscopy. A localized modification on the transmittance spectrum of VO2 film is formed due to the presence of Au nanoparticles which exhibit localized surface plasmon resonance (LSPR) in the visible-near IR region. The position of the modification wavelength region shows a strong dependence on the Au mass thickness and shifts toward the red as it increases. On the other hand, it was found that the LSPR of Au nanoparticles can be thermally tunable because of the thermochromism of the supporting material of VO2. The LSPR wavelength, lambda(SPR), shifts to the blue with increasing temperature, and shifts back to the red as temperature decreases. A fine tuning is achieved when the temperature is increased in a stepwise manner.

夏玉米冠层叶片表观自由空间的差异研究

叶片自由空间在环境与冠层养分交换间具有重要作用,是目前植物营养学研究的重要领域之一。通过盆栽试验,以不同株型夏玉米为试材,研究了不同施氮水平(N 0、0.15和0.30 g/kg)下玉米冠层叶片表观自由空间(AFS)的差异。结果表明,不同生育期叶片AFS差异极显著(P<0.01),表现为随生育期推进,叶片AFS、生物量和全氮含量均逐渐降低;除成熟期,其它各生育期品种与施氮水平对叶片AFS的影响均存在显著的交互作用。施氮对叶片生物量的影响不显著,但施氮后叶片全氮含量显著增加。不同施氮水平下,植株冠层叶片AFS也存在显著差异,中量施氮处理(即N 0.15 g/kg)植株叶片AFS值(9.49)明显高于不施氮处理(9.03),但随施氮量进一步增加,叶片AFS下降,施氮量为N 0.30 g/kg时,叶片AFS为8.62;不同施氮水平各生育期不同叶层间叶片AFS、生物量和全氮含量差异显著(P<0.05)。不同品种间叶片全氮含量和AFS存在显著差异(P<0.05),以紧凑型品种陕单902叶片AFS最大,平均为9.24,显著高于其它品种;中间型品种农大108与平展型品种陕单9号间差异不显著,二者平均分别为9.06和8.85...

HIV抗原抗体联合检测试剂盒适用于体外病毒感染p24抗原检测

比较Vironostika HIV UniformⅡAg/Ab抗原抗体联合检测试剂盒、Vironostika HIV-1 p24抗原检测试剂盒和自制p24单克隆抗体在实验室检测不同HIV毒株体外细胞感染复制的灵敏度。比较3种检测方法在抗HIV药物筛选研究中的应用。方法：HIV抗原抗体联合检测试剂盒和p24抗原检测试剂盒检测严格按说明书操作。自制p24单克隆抗体检测HIV-1 p24抗原采用Fc抗体捕捉双抗夹心法。结果: 3种检测方法检测病毒谱相同，p24抗原检测试剂盒灵敏度最高，HIV抗原抗体联合检测试剂盒其次，自制p24抗原检测法灵敏度最低。检测抗HIV药物AZT 和 IDV抑制病毒复制的剂量曲线，HIV抗原抗体联合检测试剂盒与自制p24抗原检测法均可呈现很好的“S”型剂量曲线，检测IC50一致。HIV抗原抗体联合检测试剂盒灵敏度比自制p24抗原检测法高20倍。p24抗原检测试剂盒灵敏度高但检测范围窄(5-80pg/mL)，导致剂量曲线不明显。 结论: HIV抗原抗体联合检测试剂盒适用于实验室体外病毒感染复制的p24抗原检测。

TRANSMISSION ELECTRON-MICROSCOPY STUDY OF N+-IMPLANTED SILICON ON INSULATOR BY ENERGY-FILTERED IMAGING

We report on the first study of N+ -implanted silicon on insulator by energy-filtered imaging using an Opton electron microscope CEM 902 equipped Castaing-Henry electron optical system as a spectrometer. The inelastic images, energy window set at DELTA-E = 16 eV and DELTA-E = 25 eV according to plasmon energy loss of crystal Si and of silicon nitride respectively, give much structure information. The interface between the top silicon layer and the upper silicon nitride layer can be separated into two sublayers.

两步合金法制作p-GaN高反电极

利用两步合金法获得了能与p-GaN形成良好欧姆接触的高反射电极。电极的构成采用透明电极+高反射金属方案,厚的Ag层或Al层覆盖在合金后的Ni/Au上以提高电极的反射率,以Pt和Au作为覆盖层,可有效地防止Ag的氧化和团聚,短时间的热处理有助于Ag基电极反射率的提高。可靠性测试表明Ag基电极的稳定性较好,对于Al基电极,热处理后反射镜上出现暗斑,导致其反射率的下降。最终获得了波长在460nm处反射率为74%的Ag基高反射电极。

A Novel Optical Gate by Integration of a Photodiode and an Electroabsorption Modulator

A compact and stable three-port optical gate has been successfully fabricated by monolithically integrating asimple photodiode and an electroabsorption modulator. The gate shows an excellent DC logic "and" function with differ-ent load resistors. Its dynamical characteristics without packaging have also been measured. We observed a dynamic extinc-tion ratio of over 7dB with a 950Ω load resistor and a 7mW control light power at 622Mbit/s.

蓝宝石衬底上HVPE-GaN厚膜生长

采用氢化物气相外延（HVPE）方法，以蓝宝石作衬底，分别在MOCVD-GaN模板和蓝宝石衬底上直接外延生长GaN。模板上的GaN生长表面平整、光亮，但开裂严重，其（0002）的双晶衍射半高宽最低为141；蓝宝石衬底上直接生长GaN外延层质量较差，其双晶衍射半高宽为168ff，但不发生开裂，HCl的载气流量对预反应有很大的影响，应力产生于外延层和衬底之间的界面处，界面孔洞的存在可以释放应力，减少开裂，光致发光（PL）谱中氧杂质引起强黄光发射。

Wide-Band Polarization-Independent Semiconductor Optical Amplifier Gate with Tensile-Strained Quasi-Bulk InGaAs

A semiconductor optical amplifier gate based on tensile-strained quasi-bulk InGaAs is developed. At injection current of 80mA,a 3dB optical bandwidth of more than 85nm is achieved due to dominant band-filling effect.Moreover, the most important is that very low polarization dependence of gain (＜0. 7dB),fiber-to-fiber lossless operation current (70～90mA) and a high extinction ratio (＞50dB) are simultaneously obtained over this wide 3dB optical bandwidth (1520～1609nm) which nearly covers the spectral region of the whole C band (1525～1565nm)and the whole L band (1570～ 1610nm). The gating time is also improved by decreasing carrier lifetime. The wideband polarization-insensitive SOA-gate is promising for use in future dense wavelength division multiplexing (DWDM) communication systems.

关于国家自然科学基金生物力学学科优先资助领域的思考

<正>作为我国基础研究最重要的资助机构之一,国家自然科学基金委员会自成立伊始即大力扶持生物力学新兴学科发展、培养和造就生物力学专业人才、推动生物力学研究基地建设,成绩斐然。在科学发展至二十一世纪的今天,针对国际生物力学领域及相关学科发展动态、国家中长期科技发展规划、以及生物力学学科

洛川黄土细颗粒混合矿物红外释光信号异常衰减的初步研究

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