云南基诺族及汉族特发性全面强直-阵挛性癫(癎)与CLCN2基因的相关性

目的 研究氯离子通道CLC-2基因是否与中国云南地区基诺族及汉族特发性全面强直-阵挛性癫(癎)(IGTCS)相关.方法 以14例云南西双版纳傣族自治州景洪市基诺乡基诺族IGTCS患者及其16名未发病亲属、67例云南籍汉族IGTCS患者及57名云南籍汉族健康体检者为对照,对常染色体3q26上CLCN2基因的内含子2及外显子5、19(内含子18)进行研究,采用PCR及直接基因测序技术,应用病例-对照研究法对CLCN2基因与云南基诺族及汉族IGTCS进行相关性分析.结果 CLCN2基因的内含子2及外显子5、19在病例组和对照组中均没有发现已报道的易患突变,但我们在对外显子19的序列测定过程中发现了其上游内含子18的146位上存在1个单核苷酸多态性位点:146T→C.该位点的3种基因型(TT、TC、CC)在汉族病例组(9、3、29例)和汉族对照组(22、9、26例)之间的分布差异有统计学意义(x2=16.079,P＜0.05);在基诺族组(基诺族病例组+基诺族亲属组,6、12、12例)与汉族对照组(22、9、26例)之间分布差异亦有统计学意义(x2=7.027,P＜0.05).汉族病例组与汉族对照组间TT型与非TT型基因型(分别为9、32例和22、35例)、TC型与非TC型基因型(分别为3、38例和9、48例)比较差异有统计学意义(x2=10.694,OR=4.121,P＜0.05;x2=11.592,OR=0.238,P＜0.05).结论 CLCN2基因内含子18的多态性位点146T→C可能是中国云南地区基诺族与汉族IGTCS患者的1个相关性位点,且在本组有限的样本数量研究中,此SNP位点在两个民族IGTCS患者之间的分布无民族差异.基因型TT为IGTCS的1个保护性因素,基因型TC则增加了患者的易患性.

菜花烙铁头蛇毒中一种具有激肤释放酶与纤维蛋白原溶酶活性的Jerdonase

通过DEAF Sephadex t-50阴离子交换、超细Sephadex 6100分子筛和反相高效液相CQ色谱层析，从菜花烙铁头蛇毒冻干粉中纯化出一种具有激肤释放酶活性和。纤维蛋白原溶酶活性的丝氨酸蛋白酶，命名为Jerdonase。在12 .5%胶浓度的SD6还原电泳条件下，该酶分子量大约为55 kD，在非还原电泳条件下，分子量大约为53 kD。此酶是一种糖蛋白，含有约35 .8%的中性糖。它的N末端氨基酸序列为IIGGDEENINEHPFLVALYDA，其序列和蛇毒中其他丝氨酸蛋白酶具有非常高的序列相似性。Jerdonase能够催化BAEE、 S-2238和S-2302的水解，其水解活性可被PMSF抑制，但是EDTA对此没有影响。Jerdonase能优先水解人纤维蛋白原的An链，同时伴随有微弱的B[3链水解活性。另外，此酶能够水解牛低分子量的激肤原，释放舒缓激肤。总之，所有的结果表明Jerdonase是一个具有多功能活性的蛇毒丝氨酸蛋白酶。

Jerdonase, a novel serine protease with kinin-releasing and fibrinogenolytic activity from Trimeresurus jerdonii venom

A novel kinin-releasing and fibrin (ogen)olytic enzyme termed jerdonase was purified to homogeneity from the venom of Trimeresurus jerdonii by DEAE Sephadex A-50 anion exchange, Sephadex G-100 (superfine) gel filtration and reverse-phase high performance liquid chromatography (RP-HPLC). Jerdonase migrated as a single band with an approximate molecular weight of 55 kD under the reduced conditions and 53 kD under the non-reduced conditions. The enzyme was a glycoprotein containing 35.8% neutral carbohydrate. The N-terminal amino acid sequence of jerdonase was determined to be IIGGDECNINEHPFLVALYDA, which showed high sequence identity to other snake venom serine proteases. Jerdonase catalyzed the hydrolysis of BAEE, S-2238 and S-2302, which was inhibited by phenymethylsulfonyl fluoride (PMSF), but not affected by ethylenediaminetetraacetic acid (EDTA). Jerdonase preferentially cleaved the Aalpha-chain of human fibrinogen with lower activity towards Bbeta-chain. Moreover, the enzyme hydrolyzed bovine low-molecular-mass kininogen and releasing bradykinin. In conclusion, all results indicated that jerdonase was a multifunctional venom serine protease.

Phylogenetic relationships within mammalian order Carnivora indicated by sequences of two nuclear DNA genes

Phylogenetic relationships among 37 living species of order Carnivora spanning a relatively broad range of divergence times and taxonomic levels were examined using nuclear sequence data from exon1 of the IRBP gene (approximate to1.3 kb) and first intron

贝叶斯联合模型与中国洞穴鱼类分化时间的估算(鲤科:金线鲃属)

金线鲃属分布于中国云贵高原及周边地区,包括10多种适应于洞穴生活、具有不同程度眼睛和色素退化的典型洞穴鱼类.迄今为止,金线鲃属的系统发育关系还存在很大争议.我们获得了34种金线鲃属鱼类及外类群的细胞色素b和ND4基因序列,用贝叶斯联合模型分析数据,重建了金线鲃属的系统发育关系;并用松散分子钟估算了本属及内部分支的起源时间.金线鲃属的单系性得到强烈的支持,本属可分为6个分支.此外,本研究结果还支持洞穴物种的多系起源假说以及季氏金线鲃(Sinocyclocheilus jii)的基部位置.松散分子钟估算表明,

Simulation of polarization effects in AlGaN/GaN heterojunction

A method for introducing polarization effects in the simulation of GaN-based heterojunction devices is proposed. A delta doping layer is inserted at the interface of heterojunction and the ionized donors or acceptors act as polarization induced fixed charges. Thus polarization effects can be taken into account in a traditional device simulator. Ga-face and N-face single AlGaN/GaN heterostructures are simulated, and the simulation results show that carrier confinement takes place only in the former structure while not in the latter one. The sheet density of free electrons at the interface of Ga-face AlGaN/GaN increases with the Al composition and the thickness of AlGaN. The consistence of simulation results with the experiments and calculations reported elsewhere shows that this method can effectively introduce polarization effects in the simulation of GaN-based heterojunction devices.

Performing Fast Addition and Multiplication by Transferring Single Electrons

This paper proposes novel fast addition and multiplication circuits that are based on non-binary redundant number systems and single electron (SE) devices. The circuits consist of MOSFET-based single-electron (SE) turnstiles. We use the number of electrons to represent discrete multiple-valued logic states and we finish arithmetic operations by controlling the number of electrons transferred. We construct a compact PD2,3 adder and a 12x12bit multiplier using the PD2,3 adder. The speed of the adder can be as high as 600MHz with 400nW power dissipation. The speed of the adder is regardless of its operand length. The proposed circuits have much smaller transistors than conventional circuits.

The effects of sapphire substrates processes to the LED efficiency - art. no. 68410M

We investigate the relation between the thickness of sapphire substrates and the extraction efficiency of LED. The increasing about 5% was observed in the simulations and experiments when the sapphire thickness changed from 100um to 200um. But the output power increasing is inconspicuous when the thickness is more than 200um. The structure on bottom face of sapphire substrates can enhance the extraction efficiency of GaN-based LED, too. The difference of output power between the flip-chip LED with smooth bottom surface and the LED with roughness bottom surface is about 50%, where only a common sapphire grinding process is used. But for those LEDs grown on patterned sapphire substrate the difference is only about 10%. Another kind of periodic pattern on the bottom of sapphire is fabricated by the dry etch method, and the output of the back-etched LEDs is improved about 50% than a common. case.

一种改进的OpenMP指导调度策略研究

在科学计算中,循环结构是最重要的并行对象之一.考虑到负载平衡、调度开销等多方面因素,OpenMP标准提供静态调度、动态调度、指导调度和运行时调度等不同策略.针对指导调度策略不适合递减型循环结构的问题,提出一种改进的new_guided指导调度策略,并在OMPi编译器上加以实现.New_guided调度策略的主要思想是对前半部分的循环采用静态调度,后半部分的循环采用指导调度.针对不同循环结构,在多核处理器上对不同调度策略进行评测.结果表明,在一般情况下,OpenMP默认的静态策略的调度性能最差;对于规则的循环结构和递增的循环结构,动态调度、指导调度和new_guided策略的性能差别不大;对于递减型的循环结构,动态调度和new_guided策略的性能相当,要优于指导调度策略;对于某些极不规则的随机循环结构,动态调度明显优于其他策略,new_guided策略的性能介于动态调度和指导调度之间.

空间网络数据库中移动对象的最短路线算法

在空间网络数据库中，基于位置的服务(10cation—based services，LBS)变得越来越重要，最短路线查询是最广泛的服务请求之一．假设在网络上有一个原始对象(例如：在道路上行驶的汽车)、有一个目标对象(例如：旅店、加油站、救护车等等)，那么最短路线查询就是要找到原始对象到目标对象的最短路线．最短路线算法的研究主要分为两个方面，实时计算和预计算．在预计算方面，当前的研究对网络空间中的最短路线查询提出了多种解决方式，但是这些方法中都假设目标对象是静止的．文中在原始对象和目标对象都处于移动状态时，给出了一种最短路线查询的 算法．该算法创建了一组索引结构，将计算两个动态点之间最短路线的问题转换为计算静态边之间最短路线的问题．主要关注交通网络．但是该算法不会使用任何领域相关的信息，所以能够被应用到其他的网络环境中．从计算复杂性而言，传统的Dijkstra算法时间复杂性估计是0((i+志)2)，而该算法的相应估计是0(klbi)．

一种非线性的分布式网格资源调价算法

针对网格环境下分布式异步动态调价算法存在均衡价格收敛过程缓慢、调价效率较低的缺点,提出了一种基于市场机制的非线性调价算法。该算法结合了当前超额需求和过去超额需求对资源价格变化的影响,较真实地刻画了需求变化后资源价格的波动过程。实验证明,非线性的调价算法明显地提高了价格收敛速度,降低了调价次数。