A survey of process hygiene in the Sri Lankan prawn industry. 1. Hygiene status of raw materials and products

A survey of processing hygiene in the Sri Lankan prawn industry has shown that the incoming raw material has extremely high bacterial loadings; about 50% of samples analysed had a total count in excess of 10,000,000/g. Although beheading reduces the count, ineffective temperature control during processing means that the final total count of raw, shell-on, P.U.D. and P.A.D. prawns, as well as cooked prawns, is in excess of 1,000,000/g. - the maximum level specified by many importing countries.

A survey of process hygiene in the Sri Lankan prawn industry. 2. The hygiene status of personnel

Of 262 personnel tested, 137 (52%) were found to be positive for Staphylococcus aureus. Among individual companies the prevalence of S. aureus ranged from 92% (Company No. 1) to 22% (Company No. 2). Although five companies provided a sanitiser hand-dip, this was found to be ineffective for the control of S. aureus. Provision of hand-washing facilities, of protective clothing and of toilet facilities was found to be inadequate for an export food industry.

A survey of process hygiene in the Sri Lankan prawn industry. 3. Critical control points

Of fifteen processing plants surveyed in Sri Lanka, only five were found to have a prawn process which was adequately controlled. Most common process faults were: inadequate chilling of prawns after a wash in 30°C, mains water, the use of large blocks of ice to cool prawns, and high ratios of prawns to ice. There was also ample scope for cross-contamination of the processed prawns.

A report of the Fisheries Catch Assessment Survey in the Ugandan waters of Lake Victoria for the August 2006 survey

This report presents findings of the CAS conducted in the Ugandan waters of Lake Victoria in August 2006. The results of the previous five CASs conducted under the same programme in July, August, September, and November 2005, and March 2006 are included to show the emerging trends. The results of the preceding CASs, which were hitherto estimated using raising factors from the 2004 Frame survey data, are updated in this report using raising factors based on the 2006 Frame survey data. Through regular CASs, information is building up to show the new picture of fish production in the Ugandan waters of the lake which is based on field observations. Similar surveys are simultaneously conducted in the Kenyan and Tanzanian parts of the lake which provide the lake wide perspective of fisheries production.

Survey on the quality of Jaadi available [in] Sri Lankan market

A quality of survey was conducted at the fish curing yards in a northwest coast and the southern coast in Sri Lanka. A total of 40 samples different varieties of fishes were collected from the market and jaadi curing yards and all were evaluated for the quality, fungal and insect infestation. Samples were analyzed for proximate composition chemical, microbiological and sensory quality. Thirty percent of the total analyzed samples of fish were found to be unfit for consumption. Samples collected from Negombo were found to the infected with maggots. Only 42% samples had dry matter above 50%. All the samples showed a protein content above 20%. The highest protein content was 27.92% in hurulla. Over 90% of the samples had TVN at acceptable quality limits (>40). The TBC for 33% of the samples were in the range 104-105/g range, while 48% were in the range of 107-108/g due to contamination of maggots and fungi. The Survey showed jaadi had a high level of protein in its composition. But defects of curing process such on imperfect cleaning inadequate salting resulted in low (Chemical and microbiological) quality of the product.

A report of the Fisheries Catch Assessment Survey in the Ugandan waters of Lake Victoria for the August 2007 survey

The monitoring of fish stocks as well as the magnitude, distribution and trends of fishing effort and fish catches is required for sound fisheries resources management. Conducting regular Catch Assessment Surveys (CASs) in Lake Victoria is one of the ways through which the partner states sharing the lake are generating information to contribute to the above process. The EU funded Implementation of a Fisheries Management Plan (IFMP) project for Lake Victoria through the Lake Victoria Fisheries Organisation (LVFO) is supporting the implementation of regionally harmonised CASs in Lake Victoria . The CASs under IFMP are following a statistical design laid down in Standard Operating Procedures (SOPs) agreed by the three partner states of the East African Community sharing the lake. In the Ugandan part of the lake, the CASs are carried out at 54 fish landing sites selected in the eleven riparian districts sharing the lake. The National Fisheries Resources Research Institute (NAFIRRI), Jinja; the Department of Fisheries Resources (DFR), Entebbe; and the Districts of Busia, Bugiri, Mayuge, Jinja, Mukono, Wakiso, Kampala, Mpigi, Masaka, Kalangala and Rakai jointly conduct the surveys. The CAS enumerators are recruited from the fishing communities and work under direct supervision of subcounty Fisheries Officers. NaFIRRI seeks to continue strengthening the engagement of the Beach Management Units (BMUs) and other fisheries Co-management Institutions in fisheries data collection .

On the importance of ship-bottom fouling by marine organisms: a techno-economic survey

A high attention has been paid for constant research on the preservation of materials in the marine environment. This includes all phases of design, development, applied engineering and economics which may influence the construction and operation of ships and underwater installations.

The survey on determination of malathion biomarker with genotoxicity and ecophysiological reactions in the Caspian roach (Rutilus rutilus caspicus)

In the present study possibility of Malathion biomarker with Genotoxicity and Ecophysiological reactions were determined in Caspian Roach (Rutilus rutilus caspicus). At fist LC50 value of Malathion, an organophosphate insecticide was determined. Then four groups of experimental fish (containing 30 fish in each group) were exposed to different concentrations of Malathion. e. 0, 0.01, 0.05 and 0/1 ppm respectively for 23 days and effects of Malathion on Hematological (RBC, WBC, Hb and Hct) and biochemical parameters (Glucose, triglyceride, urea, total protein and Albumin), some enzymes (SGPT, SGOT and ALP), Cortisol level, plasma cations (Na+ and K+) , histological changes (gill and liver) and finally DNA destruction were examined. Sampling was done in 3rd, 13th, 23rd days during exposure and also 30 days after recovery. Data analysis was done by SPSS (Ver.13) and graphs were drawn by Excel 2007. Results showed that WBC, RBC, Hb, Hct, some biochemical parameters and K+ of Mallation treatments were decreased significantly in compare to control group (P<0.05). Changes in enzyme were many different. No significant changes were observed in Na+ and cortisol levels (except in groups treated with 0.01 Mallation) (P>0.05). LC50 value of Malathion in Caspian Roach was 6.5 ppm. Histological examinations showed that Mallation cause tissue damages and there were more damages in longer times and in higher concentrations. Apoptotic cell and comet were observed as DNA destruction and they were more in treatments with higher Mallation concentrations for longer times.

A survey on relation of morphological, molecular and phylogenetic structure of zoanthids of the islands located in the Hormoz Strait (Hormoz, Qeshm, Larak, Hengam)

The order Zoantharia (Zoanthids) is one of the most neglected orders of cnidarians in the Persian Gulf. The present study aims to investigate the biodiversity of this order with morphological and molecular examination in the Persian Gulf. For this purpose, 123 colonies of zoanthids with variety of shape and colors have been collected from intertidal and shallow water zone of four islands, i. e. Hengam, Qeshm, Larak and Hormoz. After sampling, morphological characteristics of each specimen were recorded based on in situ photographs. Then DNA was extracted using the cetyl trimethyl ammonium bromide (CTAB) method. Both mitochondrial 16S ribosomal DNA (mt 16S rDNA) and cytochrome oxidase subunit I (COI) gene fragments were amplified and sequenced. The results of preliminary morphological identification integrated with two mitochondrial markers sequencing demonstrated the presence of five different species in this region; Zoanthus sansibaricus, Palythoa mutuki, Palythoa cf. mutuki, Palythoa tuberculosa and Neozoanthus persicus?. Although at first sight, morphological properties were not successful to delineate zoanthid species, they become reliable criteria to identify and delineate species in field studies after molecular identification.

Determination of bioaccumulation and microscopic survey of LAS pollutant effect on the liver, kidney and gill tissues of Rutilus frisii kutum in Caspian sea

Linear alkylbenzene sulfonate (LAS) are widely used in detergent industry. Due to contaminants entering the water, and the effects of their accumulation in fish, LAS, has a great importance in environmental pollution. In the present study, accumulation of LAS and its histological effects on gill tissue, liver and kidney of Caspian kutum (Rutilus frisii kutum) were studied. Caspian kutum is the most important and most valuable teleosts of the Caspian Sea. Due to releasing Caspian Kutum in rivers and Anzali Lagoon and unlimited entry of wastewater to the aquatic ecosystem, research on the impact of LAS on Caspian kutum is important. In the present study, fish exposed to sublethal concentrations of LAS (0.58, 1.16 and 2.32 mg/l) for 192 hours. Control treatments with three replicates at 0, 24, 48, 72, 96 and 192 hours were done. For assessments of the histological effects of LAS, tissue sections prepared and by using Hematoxylin - Eosin were stained, then the prepared sections, examined by light microscopy. For determination of the bio accumulation of LAS, the soxhlet extraction and solid phase extraction was performed to determine the amount of LAS using HPLC with fluorescence detector. According to results average of bioconcentration factor and LAS concentrations in fish had reached stable levels after approximately 72 h and thus represented steady state BCF values in this species. The value of steady-state bio-concentration factor of total LAS was 33.96 L.Kg- 1 and for each of the homologous C10-n-LAS, C11-n-LAS, C12-n-LAS and C13-n- LAS were 3.84, 6.15, 8.58 and 15.57 L.Kg-1 respectively. According to the results obtained in gills exposed to LAS, histopathological alteration include hypertrophy, lifting of lamella epithelium, edema, clubbing of lamellae hyperplasia, lamellar fusion and aneurysm were seen. In liver tissue exposed to three concentrations of LAS, congestion and dilation of sinusoids, irregular-shaped nuclei and degeneration in the hepatocyte, vacuolar degeneration and necrosis were observed. In kidney exposed to three concentrations of LAS, reduction of the interstitial haematopoietic tissue, degeneration in the epithelial cells of renal tubule, tubular degeneration, necrosis, shrinkage and luminal occlusion were observed. According to the results the most alteration due to exposure to LAS was seen in the gill tissue. None of the control samples showed histological effects of LAS.

The survey on growth and survival rate parameters with slurry and comparison by common method

This project was on “Study of Slurry, as an enrichment compound, on growth and survival parameters of Rutilus firisii kutum Kamensky 1901 larva and compare with routine condition according to hypothesis that use of Slurry, fertile organic compound, to increase the efficiency of survivorship and growth of Rutilus firisii and in other hand adaptation of natural food was performance.” The object of this project is to compare growth of Rutilus firisii and in usual condition and the condition of use Slurry The experiences performed in culture and propagation center Dr. Yousefpour decent (Associan of Dr. Beheshti culture and propagation) in Siahkal village, 32 Km far from southeast, in North of Iran. In this plan, three different treatments and related these nine pools , in 1.7 hec area (in same condition) were determined so that the density of storage was 1.7 million/hec larva. In this research at first treatment m we use slurry as enrichment compound during larval within a period of 13 days, and it used all the days of during larval in second treatment. Then the result of this study compared to control treatment. The results have shown the level of this study compared to control treatment. The results have shown the level of survivorship in pools were nutrition by slurry was 1.7 million times or twice than pools were nutrition with usual concentrate. In addition, growth coefficient such as daily weight growing (DWG) index, daily length growth (DLG), specific growth ratio (SGR) were measured in this case. All of these parameters in slurry treatment were shown noticeable enhancement in first treatment Thereof in the first week there was significant difference (P<0.001) between the average of length and weight in first week , and in the second week there was a significant difference (P<0.05) between them.

Survey of plankton blooms and red tides and their interaction with environmental factors through monitoring satellite images

One of the most important marine ecological phenomena is red tide which is created by increasing of phytoplankton population, influenced by different factors such as climate condition changes, utrification hydrological factors and can leave sever and undesired ecological and economical effects behind itself in the case of durability. Coast line of Hormozgan is about 900km from east to west, within the range of geographical coordinates of 56 16 23.8, 26 58 8.8 to 54 34 5.33 and 26 34 32 eastern longitude and northern latitude, seven sampling stations were considered and sampled for a period of one year from October 2008 to October 2009. after the analysis of Satellite images, monthly, during the best time. In several stages, samplings were performed. In each station, three samples were collected for identification and determination of Bloom- creating species abundance. Cochlodinium polykrikoides was the species responsible for the discoloration which occurred at October 2008 in Hormozgan marine water. Environmental parameters such as sea surface temperature, pH, salinity, Dissolved Oxygen concentration, Total Dissolved Solids (T.D.S.), conductivity, nitrate, nitrite and phosphate and also chlorophyll a were measured and calculated. Kruscal Wallis test was used to compare the densities between different months, seasons and the studied stations. Mann-whitney test from Nonparametric Tests was used for couple comparison. Pearson correlation coefficient was used to determine the relationship between physical and chemical data set and the abundance of Cochlodinium polykrikoides. Multivariate Regression and analysis of variance (ANOVA) also were used to obtain the models and equations of red tide occurrence relationship, environmental parameters and nutrient data. The highest density was 26 million cells per liter in Qeshm station. A meaningful difference was observed between sampling months and seasons but there was no between sampling stations which indicates that in favorable conditions, the occurrence of this phenomenon by the studied species is probable. Regarding to β coefficients of nitrate, temperature, phosphate, Total Dissolvable Solutions (T.D.S) and pH these parameters are effective on the abundance of this species and red tide occurrence. Increase in these factors can represent the effects and outcomes of human activities and increase in marine pollution.

Report of frame survey of the Kyoga basin lakes 2002

A comprehensive Frame survey was carried out in lakes Kwania, Kyoga and the Kyoga basin minor lakes which include Lake Sisina in 2002 (Figure 1). The Frame survey was coordinated by the Department of Fisheries Resources (DFR) assisted by the National Fisheries Resources Research Institute (NAFIRRI) with technical support. The riparian districts through the sub-county fisheries offices and the BMUs provided the enumerators and supervisors. The frame survey captured all the important characteristics of the fisheries and facilities supporting the fisheries and thus provides a strong baseline for future reference of management interventions in the basin.

Survey of shark fisheries and preparation of a National Plan of Action (NPOA) for conservation and management of shark resources in Bangladesh

The report presents; terms of reference; work progress; surveys of shark fishers and traders; shark biodiversity survey; and a National Plan of Action (NPOA) for conservation and management of shark resources in Bangladesh.

Report of the survey of shark fisheries for conservation and management of shark resources, Sri Lanka

A shark survey was conducted between October, 2012 and December, 2013 in order to strengthen the existing data collection and research thus improving conservation and management of sharks in Sri Lanka.