Identification and validation of candidate Myb target genes
Data(s) |
01/01/2001
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Resumo |
While a considerable number of candidate Myb target genes have been reported to date, most of these are likely to play little or no role in transformation by myb oncogenes. Here we have used a conditionally myb-transformed myeloid cell line (ERMYB) to further examine Myb regulation of one candidate target gene-c-myc-that has the potential to affect cell proliferation. It was found that the major influence on c-myc expression was the presence of cytokine (GM-CSF) rather than Myb activity. We also describe the application of PCR-based subtractive hybridization and low-density cDNA array screening, in conjunction with the ERMYB line, to the identification of additional Myb target genes. Preliminary identification of a number of candidates is reported; these include myeloperoxidase, which is known to have essential Myb-binding sites in its regulatory region. (C) 2001 Academic Press. |
Identificador | |
Publicador |
Academic Press Inc |
Palavras-Chave | #Hematology #Myb #Transformation #Target Genes #Rda #Cdna Array #Northern Blotting #C-myb #Hematopoietic-cells #Binding-sites #Myeloid Cells #Differentiation #Transcription #Promoter #Oncoprotein #Repression #Expression |
Tipo |
Journal Article |