La pedagog??a del Colegio de la Concepci??n de Hu??rfanos : una aproximaci??n

Resumen tomado de la publicaci??n

Valores y educaci??n en el Colegio menor de la Concepci??n de Hu??rfanos de Salamanca durante el siglo XVI

Resumen tomado de la publicaci??n

'Corte y confecci??n' de un curr??culum para hospicianas

Resumen basado en la publicaci??n

Pobreza y ni??os marginados en la Edad Moderna

Resumen tomado de la revista

El ni??o exp??sito en el Despotismo Ilustrado : su crianza y su educaci??n

Resumen tomado de la revista

Las casas de correcci??n en el siglo XIX espa??ol : notas para su estudio

Resumen tomado de la revista

Ordenanzas de la Casa de Misericordia de Sig??enza de 1777

Este art??culo recoge un modelo de legislaci??n institucional que es ejemplo de todas las circunstancias e ideas presentes en el pensamiento ilustrado sobre acogida de ni??os hu??rfanos o de pobres vagabundos. Se detalla en ellas todos los aspectos que regularon la Casa de Misericordia desde la entrada en ella de los ni??os hu??rfanos y vagabundos pertenecientes al Obispado de Siguenza, como la organizaci??n interna de la misma, la educaci??n en las primeras letras de los ni??os y la ense??anza de un oficio.

El movimiento higienista en Espa??a a finales del siglo XIX y su proyecci??n en las colonias escolares de vacaciones de la Diputaci??n Provincial de Valladolid : 1925-1936

Analizar las colonias escolares en Espa??a y sus antecedentes. Analizar concretamente las colonias escolares de vacaciones de la Diputaci??n Provincial de Valladolid. Memorias de las instituciones, informes que enviaban a las Juntas Provinciales de Protecci??n a la Infancia y Represi??n de la Mendicidad,la revista El monitor sanitario, actas de las sesiones celebradas por los diputados provinciales, documentos diversos conservados en el archivo de la Diputaci??n Provincial de Valladolid. Investigaci??n bibliogr??fica y documental. Se destaca la aportaci??n del movimiento higienista al establecimiento de las colonias escolares como medio para prevenir y remediar los males que acechan a la infancia desde la propia acci??n educadora combinando higiene y pedagog??a. La Diputaci??n de Valladolid, en cuanto instituci??n protectora de la infancia m??s necesitada contribuy?? de manera destacada con el establecimiento de colonias escolares en el Pabell??n de las Salinas en Medina del Campo que acog??a a los hospicianos y que potenciaban en ellos la formaci??n desde una doble acci??n: higienico-social y sanitario-pedag??gica. Su extensi??n en el tiempo 1924/1925-1985 hicieron de este movimiento colonial, una obra casi ??nica en el escenario espa??ol de las colonias escolares de vacaciones. Esta investigaci??n recibi?? el 3er Premio a la Investigaci??n de la Diputaci??n Provincial de Valladolid en la convocatoria de 1992.

An (O)over-tilde(mn) Gomory-Hu Tree Construction Algorithm for Unweighted Graphs

We present a fast algorithm for computing a Gomory-Hu tree or cut tree for an unweighted undirected graph G = (V, E). The expected running time of our algorithm is (O) over tilde (mc) where vertical bar E vertical bar = m and c is the maximum u-v edge connectivity, where u, v is an element of V. When the input graph is also simple (i.e., it has no parallel edges), then the u-v edge connectivity for each pair of vertices u and v is at most n - 1; so the expected run-ning time of our algorithm for simple unweighted graphs is (O) over tilde (mn). All the algorithms currently known for constructing a Gomory-Hu tree [8, 9] use n - 1 minimum s-t cut (i.e., max flow) subroutines. This in conjunction with the current fastest (O) over tilde (n(20/9)) max flow algorithm due to Karger and Levine[11] yields the current best running time of (O) over tilde (n(20/9)n) for Gomory-Hu tree construction on simple unweighted graphs with m edges and n vertices. Thus we present the first (O) over tilde (mn) algorithm for constructing a Gomory-Hu tree for simple unweighted graphs. We do not use a max flow subroutine here; we present an efficient tree packing algorithm for computing Steiner edge connectivity and use this algorithm as our main subroutine. The advantage in using a tree packing algorithm for constructing a Gomory-Hu tree is that the work done in computing a minimum Steiner cut for a Steiner set S subset of V can be reused for computing a minimum Steiner cut for certain Steiner sets S' subset of S.

DNA Clasping by Mycobacterial HU: The C-Terminal Region of HupB Mediates Increased Specificity of DNA Binding

Background: HU a small, basic, histone like protein is a major component of the bacterial nucleoid. E. coli has two subunits of HU coded by hupA and hupB genes whereas Mycobacterium tuberculosis (Mtb) has only one subunit of HU coded by ORF Rv2986c (hupB gene). One noticeable feature regarding Mtb HupB, based on sequence alignment of HU orthologs from different bacteria, was that HupB(Mtb) bears at its C-terminal end, a highly basic extension and this prompted an examination of its role in Mtb HupB function. Methodology/Principal Findings: With this objective two clones of Mtb HupB were generated; one expressing full length HupB protein (HupB(Mtb)) and another which expresses only the N terminal region (first 95 amino acid) of hupB (HupB(MtbN)). Gel retardation assays revealed that HupBMtbN is almost like E. coli HU (heat stable nucleoid protein) in terms of its DNA binding, with a binding constant (K-d) for linear dsDNA greater than 1000 nM, a value comparable to that obtained for the HU alpha alpha and HU alpha beta forms. However CTR (C-terminal Region) of HupB(Mtb) imparts greater specificity in DNA binding. HupB(Mtb) protein binds more strongly to supercoiled plasmid DNA than to linear DNA, also this binding is very stable as it provides DNase I protection even up to 5 minutes. Similar results were obtained when the abilities of both proteins to mediate protection against DNA strand cleavage by hydroxyl radicals generated by the Fenton's reaction, were compared. It was also observed that both the proteins have DNA binding preference for A: T rich DNA which may occur at the regulatory regions of ORFs and the oriC region of Mtb. Conclusions/Significance: These data thus point that HupB(Mtb) may participate in chromosome organization in-vivo, it may also play a passive, possibly an architectural role.

An O(mn) Gomory-Hu Tree Construction Algorithm for Unweighted Graphs

We present a fast algorithm for computing a Gomory-Hu tree or cut tree for an unweighted undirected graph G = (V,E). The expected running time of our algorithm is Õ(mc) where |E| = m and c is the maximum u-vedge connectivity, where u,v ∈ V. When the input graph is also simple (i.e., it has no parallel edges), then the u-v edge connectivity for each pair of vertices u and v is at most n-1; so the expected running time of our algorithm for simple unweighted graphs is Õ(mn).All the algorithms currently known for constructing a Gomory-Hu tree [8,9] use n-1 minimum s-t cut (i.e., max flow) subroutines. This in conjunction with the current fastest Õ(n20/9) max flow algorithm due to Karger and Levine [11] yields the current best running time of Õ(n20/9n) for Gomory-Hu tree construction on simpleunweighted graphs with m edges and n vertices. Thus we present the first Õ(mn) algorithm for constructing a Gomory-Hu tree for simple unweighted graphs.We do not use a max flow subroutine here; we present an efficient tree packing algorithm for computing Steiner edge connectivity and use this algorithm as our main subroutine. The advantage in using a tree packing algorithm for constructing a Gomory-Hu tree is that the work done in computing a minimum Steiner cut for a Steiner set S ⊆ V can be reused for computing a minimum Steiner cut for certain Steiner sets S' ⊆ S.

Targeting Mycobacterium tuberculosis nucleoid-associated protein HU with structure-based inhibitors

The nucleoid-associated protein HU plays an important role in maintenance of chromosomal architecture and in global regulation of DNA transactions in bacteria. Although HU is essential for growth in Mycobacterium tuberculosis (Mtb), there have been no reported attempts to perturb HU function with small molecules. Here we report the crystal structure of the N-terminal domain of HU from Mtb. We identify a core region within the HU-DNA interface that can be targeted using stilbene derivatives. These small molecules specifically inhibit HU-DNA binding, disrupt nucleoid architecture and reduce Mtb growth. The stilbene inhibitors induce gene expression changes in Mtb that resemble those induced by HU deficiency. Our results indicate that HU is a potential target for the development of therapies against tuberculosis.

New Oncogenic Drivers in Hepatocellular Carcinoma: Role of the RNA Binding Protein Hu antigen R

156 p. : graf.