Angelica archengelica extract induced perturbation of rat skin and tight junctional protein (ZO-1) of HaCaT cells

Background and purpose of the study: Herbal enhancers compared to the synthetic ones have shown less toxis effects. Coumarins have been shown at concentrations inhibiting phospoliphase C-Y (Phc-Y) are able to enhance tight junction (TJ) permeability due to hyperpoalation of Zonolous Occludense-1 (ZO-1) proteins. The purpose of this study was to evaluate the influence of ethanolic extract of Angelica archengelica (AA-E) which contain coumarin on permeation of repaglinide across rat epidermis and on the tight junction plaque protein ZO-1 in HaCaT cells. Methods: Transepidermal water loss (TEWL) from the rat skin treated with different concentrations of AA-E was assessed by Tewameter. Scanning and Transmission Electron Microscopy (TEM) on were performed on AA-E treated rat skin portions. The possibility of AA-E influence on the architecture of tight junctions by adverse effect on the cytoplasmic ZO-1 in HaCaT cells was investigated. Finally, the systemic delivery of repaglinide from the optimized transdermal formulation was investigated in rats. Results: The permeation of repaglinide across excised rat epidermis was 7-fold higher in the presence of AA-E (5% w/v) as compared to propylene glycol:ethanol (7:3) mixture. The extract was found to perturb the lipid microconstituents in both excised and viable rat skin, although, the effect was less intense in the later. The enhanced permeation of repaglinide across rat epidermis excised after treatment with AA-E (5% w/v) for different periods was in concordance with the high TEWL values of similarly treated viable rat skin. Further, the observed increase in intercellular space, disordering of lipid structure and corneocyte detachment indicated considerable effect on the ultrastructure of rat epidermis. Treatment of HaCaT cell line with AA-E (0.16% w/v) for 6 hrs influenced ZO-1 as evidenced by reduced immunofluorescence of anti-TJP1 (ZO-1) antibody in Confocal Laser Scanning Microscopy studies (CLSM) studies. The plasma concentration of repaglinide from transdermal formulation was maintained higher and for longer time as compared to oral administration of repaglinide. Major conclusion: Results suggest the overwhelming influence of Angelica archengelica in enhancing the percutaneous permeation of repaglinide to be mediated through perturbation of skin lipids and tight junction protein (ZO-1).

Beza? beleg a zo kargus koulz 'vel beza? relijiuz: Ur sell ouzh eus an Iliz, ar gloer, ar veleien hag ar relijiuzed er gwerzio? hag er sonio? brezhonek, gant evezhiadenno? war un nebeud liammo? etre an taolennadur-se hag an hengoun kembreat

Hincks, R. (2007). Beza? beleg a zo kargus koulz 'vel beza? relijiuz: Ur sell ouzh eus an Iliz, ar gloer, ar veleien hag ar relijiuzed er gwerzio? hag er sonio? brezhonek, gant evezhiadenno? war un nebeud liammo? etre an taolennadur-se hag an hengoun kembreat. Roazhon: hor Yezh.

2-D ZO CRS stack by considering an acquisition line with smooth topography

Os dados sísmicos terrestres são afetados pela existência de irregularidades na superfície de medição, e.g. a topografia. Neste sentido, para obter uma imagem sísmica de alta resolução, faz-se necessário corrigir estas irregularidades usando técnicas de processamento sísmico, e.g. correições estáticas residuais e de campo. O método de empilhamento Superfície de Reflexão Comum, CRS ("Common-Reflection-Surface", em inglês) é uma nova técnica de processamento para simular seções sísmicas com afastamento-nulo, ZO ("Zero-Offset", em inglês) a partir de dados sísmicos de cobertura múltipla. Este método baseia-se na aproximação hiperbólica de tempos de trânsito paraxiais de segunda ordem referido ao raio (central) normal. O operador de empilhamento CRS para uma superfície de medição planar depende de três parâmetros, denominados o ângulo de emergência do raio normal, a curvatura da onda Ponto de Incidência Normal, NIP ("Normal Incidence Point", em inglês) e a curvatura da onda Normal, N. Neste artigo o método de empilhamento CRS ZO 2-D é modificado com a finalidade de considerar uma superfície de medição com topografia suave também dependente desses parâmetros. Com este novo formalismo CRS, obtemos uma seção sísmica ZO de alta resolução, sem aplicar as correições estáticas, onde em cada ponto desta seção são estimados os três parâmetros relevantes do processo de empilhamento CRS.

Zoë Wicomb's Ghosts. Uncanny Translocations in David's Story and The One That Got Away

This essay analyses Zoë Wicomb's novel David's Story and her latest collection of short stories, The One That Got Away, through the lense of cosmopolitanism and Jacques Derrida's concept of ‘hauntology’. Wicomb is a cosmopolitan author in a very precise sense: an author who embeds locally specific stories in a complex intertextual, historical and transnational web of cross-references. As settings, characters and objects move between Scotland and South Africa, it appears that the histories of these countries are mutually haunted by each other. Uncanny encounters with the past, and with memorials and art objects that take on a spectral quality, evoke an increasing sense of disorientation on the part of protagonists and readers alike. Assumptions about place, history and identity are thus constantly undermined and reconfigured.

Hisṭorie fon der šehne kenigliche printzesin Helene welche durch eini wasr fahrṭ zo fr unglikṭi ds zi muzṭ ir brohṭ beṭlin gehn

Digitalisat der Ausg. Frankfurt an der Oder, 1782/83

Taḥanûnê benôt Yešûrûn : ein gebeṭbuch fir Izraels froien und medchen tzur erwekkung der andachṭ an wochen fezṭ und fazṭṭagen zo wi oich in allen ferhelṭnizzen dez lebens

Digitalisat der Ausg. Fürth, 1859/60

Ḥuḳe ḥayim : enṭhalṭend di vikhṭigsṭen ferordnungen iber das yidishe leben im allgemeinen, zo ṿi iber tsitsit, tefilin, tefilah und di ibrigen gottesdienstlikhen gebroykhe

fon Daṿid Ḳassel

Ṭroierede oif den bṭribtṣṭe ṭodesfal wailand ihri keizr keniglichi aposṭoliši maiesṭeṭ Maria Ṭerezia : in judišn mundarṭ ghalṭin in der zo ginanṭin Maizlś šul tzu Prag ... den 10 detzembr 1780 ... oif dem ṭaiṭši ibrzetzṭ ...

... fon ... Jeḥezkel Landa

Sēfer Divrê šālôm we-emet : rajones un erklerungen fun dem zo groisin eivigen glik ...

mē'ēt Šālôm Yiṣḥāq Lēwîṭan

Zoé Prévost als Madeleine, Rollenbild, in: Adolphe Adam: Le Postillon de Lonjumeau

Signatur des Originals: S 36/G11934

Zoé Prévost, Rollenbild, Brustbild

Signatur des Originals: S 36/G03520

Zoé Prévost, Rollenbild, Brustbild

Signatur des Originals: S 36/G03521

Zoé Prévost als Isabelle, Rollenbild, Hüftbild, in: Ferdinand Herold: Le Pré aux clercs

Signatur des Originals: S 36/G04318