Androgen receptor targets NFKB and TSPI to suppress prostate tumor growth in vivo

The androgen role in the maintenance of prostate epithelium is subject to conflicting opinions. While androgen ablation drives the regression of normal and cancerous prostate, testosterone may cause both proliferation and apoptosis. Several investigators note decreased proliferation and stronger response to chemotherapy of the prostate cancer cells stably expressing androgen receptor (AR), however no mechanistic explanation was offered. In this paper we demonstrate in vivo anti-tumor effect of the AR on prostate cancer growth and identify its molecular mediators. We analyzed the effect of AR on the tumorigenicity of prostate cancer cells. Unexpectedly, the AR-expressing cells formed tumors in male mice at a much lower rate than the AR-negative controls. Moreover, the AR-expressing tumors showed decreased vascularity and massive apoptosis. AR expression lowered the angiogenic potential of cancer cells, by increasing secretion of an anti-angiogenic protein, thrombospondin-1. AR activation caused a decrease in RelA, a subunit of the pro-survival transcription factor NF kappa B, reduced its nuclear localization and transcriptional activity. This, in turn, diminished the expression of its anti-apoptotic targets, Bcl-2 and IL-6. Increased apoptosis within AR-expressing tumors was likely due to the NF kappa B suppression, since it was restricted to the cells lacking nuclear (active) NF kappa B. Thus we for the first time identified combined decrease of NF kappa B and increased TSP1 as molecular events underlying the AR anti-tumor activity in vivo. Our data indicate that intermittent androgen ablation is preferable to continuous withdrawal, a standard treatment for early-stage prostate cancer. (C) 2007 Wiley-Liss, Inc.

Análisis de la calidad y productividad en el desarrollo de un proyecto software en una microempresa con TSPi

Este artículo presenta el análisis de los resultados obtenidos al aplicar TSPi en el desarrollo de un proyecto software en una microempresa desde el punto de vista de la calidad y la productividad. La organización en estudio necesitaba mejorar la calidad de sus procesos pero no contaba con los recursos económicos que requieren modelos como CMMI-DEV. Por esta razón, se decidió utilizar un proceso adaptado a la organización basado en TSPi, observándose una reducción en la desviación de las estimaciones, un incremento en la productividad, y una mejora en la calidad.---ABSTRACT---This article shows the benefits of developing a software project using TSPi in a “Very Small Enterprise” based in quality and productivity measures. An adapted process from the current process based on the TSPi was defined and the team was trained in it. The workaround began by gathering historical data from previous projects in order to get a measurement repository, and then the project metrics were collected. Finally, the process, product and quality improvements were verified.

Quality and productivity analysis in a very small enterprise using TSPi

This article shows the benefits of developing a software project using TSPi in a "Very Small Enterprise" based in quality and productivity measures. An adapted process from the current process based on the TSPi was defined and the team was trained in it. The pilot project had schedule and budget constraints. The workaround began by gathering historical data from previous projects in order to get a measurement repository, and then the project metrics were collected. Finally, the process, product and quality improvements were verified

A small settings case study using TSPi in a software project

This article introduces a small setting case study about the benefits of using TSPi in a software project. An adapted process from the current process based on the TSPi was defined. The pilot project had schedule and budget constraints. The process began by gathering historical data from previous projects in order to get a measurement repository. The project was launched with the following goals: increase the productivity, reduce the test time and improve the product quality. Finally, the results were analysed and the goals were verified

Benefits of TSPi in a software project under a small settings enviroment

This article presents a case study about the TSPi benefits in a software project under a Small Settings environment. An adapted process based on the TSPi was defined. The pilot project had a schedule and budget restricted. The process began collecting historical projects data in order to get a measure repository. The project was launched defining the following goals: increase the productivity, reduce the test time and improve the product quality. Finally, the results were analysed and the goals were verified.

A Small Settings Case Study using TSPi in a Software Project

This article introduces a small setting case study about the benefits of using TSPi in a software project. An adapted process from the current process based on the TSPi was defined. The pilot project had schedule and budget constraints. The process began by gathering historical data from previous projects in order to get a measurement repository. The project was launched with the following goals: increase the productivity, reduce the test time and improve the product quality. Finally, the results were analysed and the goals were verified.