The Effect of TAK-778 on Gene Expression of Osteoblastic Cells Is Mediated Through Estrogen Receptor

This study evaluated the effect of TAK-778 [(2R, 4S)-(-)-N-(4-diethoxyphosphorylmethylphenyl)-1,2,4,5-tetrahydro-4-methyl-7,8-methylenedioxy-5-oxo-3-benzothiepin-2-carboxamide)] on in vitro osteogenic events and on gene expression of osteoblastic cells derived from human alveolar bone and the participation of estrogen receptors (ERs) on such effect. Osteoblastic cells were subcultured, with or without TAK-778 (10(-5) M), to evaluate cell growth and viability, total protein content, and alkaline phosphatase (ALP) activity at 7, 14, and 21 days; bone-like formation at 21 days; and gene expression, using cDNA microarray, at 7 days. Also, osteoblastic cells were exposed to TAK-778 (10-5 M) combined to ICI182,780, a nonspecific ER antagonist (10(-6) M), and gene expression was evaluated by real-time polymerase chain reaction (PCR) at 7 days. TAK-778 induced a reduction in culture growth and an increase in cell synthesis, ALP activity, and bone-like formation. The cDNA microarray showed genes associated with cell adhesion and differentiation, skeletal development, ossification, and transforming growth factor-P receptor signaling pathway, with a tendency to be higher expressed in cells exposed to TAK-778. The gene expression of ALP, osteocalcin, Msh homeobox 2, receptor activator of NF-kappa B ligand, and intercellular adhesion molecule 1 was increased by TAK-778 as demonstrated by real-time PCR, and this effect was antagonized by ICI182,780. The present results demonstrated that TAK-778 acts at a transcriptional level to enhance the in vitro osteogenic process and that its effect on gene expression of osteoblastic cells is mediated, at least partially, through ERs. Based on these findings, TAK-778 could be considered in the treatment of bone metabolic disorders. Exp Biol Med 234:190-199, 2009

Om tak-skifwers upletande, igen-kännande och nytta

Invocatio: I.I.N.

TAK, an HIV Tat-associated kinase, is a member of the cyclin-dependent family of protein kinases and is induced by activation of peripheral blood lymphocytes and differentiation of promonocytic cell lines

We have previously identified a cellular protein kinase activity termed TAK that specifically associates with the HIV types 1 and 2 Tat proteins. TAK hyperphosphorylates the carboxyl-terminal domain of the large subunit of RNA polymerase II in vitro in a manner believed to activate transcription [Herrmann, C. H. & Rice, A. P. (1995) J. Virol. 69, 1612–1620]. We show here that the catalytic subunit of TAK is a known human kinase previously named PITALRE, which is a member of the cyclin-dependent family of proteins. We also show that TAK activity is elevated upon activation of peripheral blood mononuclear cells and peripheral blood lymphocytes and upon differentiation of U1 and U937 promonocytic cell lines to macrophages. Therefore, in HIV-infected individuals TAK may be induced in T cells following activation and in macrophages following differentiation, thus contributing to high levels of viral transcription and the escape from latency of transcriptionally silent proviruses.

Placki - ciasta - desery : książka ta zawiera najnowsze amerykańskie przepisy na rozmaite placki, desery, konfekcye, także objaśnienia kilkunastu wyrazów polsko-angielskich /

Sample recipes: Sunshine cake, Placek na klonowym cukrze, Placek czekoladowy (Devils food), Pączki.

Drōshin tak : grakan uruagitsner /

A. Anonkʻ or inkan - -B. Anonkʻ or kěmnan.

Sefer Shaʻare torat ha-taḳanot : hu meʼasef le-khol maḥanot ha-taḳanot asher yesodotan mi-yemot [sic] Mosheh, avi ha-neviʼim ʻad ḥatimat ha-Talmud /

v.2 designated as ḥeleḳ rishon, maḥberet shenyah on Hebrew t.p., but as 2. band, 1. theil on added German t.p.

Tak govoril Zaratustra; kniga dli︠a︡ vsi︠e︡kh i ni dli︠a︡ kogo.

Translation of his Also sprach Zarathustra.

TAK VAM TREBA

Mode of access: Internet.

Sefer Zivḥe tsedeḳ : ʻal Shulḥan ʻarukh Yoreh deʻah ... taḳanot be-ʻinyene hu-sheḥitah ... gam ʻal ḥilkhot ribit ṿe-taḳanat ʻagunot ... ṿe-sheʼelot u-teshuvot mefuzarim ...

Vol.2 includes Sheʼelot u-teshuvot Oraḥ ḥayim, Yoreh deʻah, Even ha-ezer, Ḥoshen ha-mishpat 119 p. at end.

Ho batarias ; Ho Takē-Ploumas ; Bayrōn /

Mode of access: Internet.

De landbouw als tak van staatszorg ....

Proefschrift--Utrecht.

Sochinenīi︠a︡ i perevody kak stīkhami tak i prozoi︠u︡ /

Svodnyĭ katalog, item no. 7344.

Polska w apostazii czyli w tak zwanym russo-sławianizmie i w apoteozie czyli w tak zwanym gallo-kosmopolityzmie jako warunkach załozenia i rozwiązania problematu etnologicznego w praktyce i wiedzy.

Mode of access: Internet.

Akta grodzkie i ziemskie z c zasów Rzeczypospolitej Polskiej z Archiwum tak zwanego bernardyńskiego we Lwowie,

Imprint of v. 2-14: Lwów, Glówny skład W ksie̜g. Seyfartha i Czajkowskiego.