Seismic-resistant building practices resulting from local seismic culture

Considering that vernacular architecture may bear important lessons on hazard mitigation, this chapter focuses on the European Mediterranean countries and studies traditional seismic-resistant architectural elements and techniques that local populations developed to prevent or repair earthquake damage. This area was selected as a case study because, as a highly seismic region, it has suffered the effect of many earthquakes along the history and, thus, regions within this area are prone to have developed a Local Seismic Culture. After reviewing seismic resistant construction concepts, a wide range of traditional construction solutions that, in many cases, have shown to improve the seismic performance of vernacular constructions of these regions is presented, as a contribution to the general overview of retrofitting building systems provided in this book. The main motivation is that most of these techniques can be successfully applied to preserve and to retrofit surviving examples without prejudice for their identity.

Evaluation of seismic vulnerability assessment parameters for portuguese vernacular constructions with nonlinear numerical analysis

Considering that vernacular architecture may bear important lessons on hazard mitigation and that well-constructed examples showing traditional seismic resistant features can present far less vulnerability than expected, this study aims at understanding the resisting mechanisms and seismic behavior of vernacular buildings through detailed finite element modeling and nonlinear static (pushover) analysis. This paper focuses specifically on a type of vernacular rammed earth constructions found in the Portuguese region of Alentejo. Several rammed earth constructions found in the region were selected and studied in terms of dimensions, architectural layout, structural solutions, construction materials and detailing and, as a result, a reference model was built, which intends to be a simplified representative example of these constructions, gathering the most common characteristics. Different parameters that may affect the seismic response of this type of vernacular constructions have been identified and a numerical parametric study was defined aiming at evaluating and quantifying their influence in the seismic behavior of this type of vernacular buildings. This paper is part of an ongoing research which includes the development of a simplified methodology for assessing the seismic vulnerability of vernacular buildings, based on vulnerability index evaluation methods.

Seismic behaviour of portuguese rammed earth buildings

Rammed earth is one of the oldest building materials in the world and is present in the Portugal with a particular focus in the South of the country. The mechanical properties and the structural behaviour of rammed earth constructions have been the subject of study of many researchers in the recent years. This study is part of a broader research on vernacular seismic culture in Portugal. Numerical analyses were carried out on a rammed earth masonry construction representative of the vernacular heritage of Alentejo region. Variations in the geometry, constructive characteristics and material properties were implemented and the main conclusions of the non-linear static and modal analysis are presented. Analysing the damage framework allowed interpreting the weaknesses of this type of constructions and consider the most appropriate reinforcement methodologies.

Seismic behaviour assessment of vernacular isolated buildings

This paper presents the numerical seismic analysis of isolated vernacular buildings characteristic of the Alentejo region, which is considered a medium seismic hazard region in Portugal.A representative isolated building was selected from a database, and a geometric model was defined for the numerical pushover analysis. Subsequently, a parametric analysis was carried out to assess the influence of distinct parameters on the seismic behaviour of such buildings.

Seismic vulnerability of vernacular buildings in urban centres: the case of Vila Real de Santo António

Following detailed studies of Portuguese vernacular building typologies, this paper deals with buildings located in historical urban centres. An analysis of the history of the urban centre and, in particular, of some vernacular buildings is enhanced. Additionally, a discussion on the influence of changes of the geometry, and on added built volumes to original buildings in the seismic vulnerability of the buildings is also provided.

Local seismic cultures: the use of timber frame structures in the south of Portugal

Given the fact that using timber frame structures has proven to improve the seismic behavior of vernacular architecture, as has been reported in past earthquakes in many countries, its preservation as a traditional earthquake resistant practice is important. This paper firstly intends to evaluate whether the use of timber frames as a traditional seismic resistant technique for vernacular architecture in the South of Portugal, traditionally a seismic region, is still active. Secondly, the city of Vila Real de Santo António was selected as a case study because it also followed a Pombaline development contemporary to the reconstruction of Lisbon. The plan included the provision of timber frame partition walls for some of the buildings and, thus, an overview of the type of constructions originally conceived is provided. Finally, the alterations done in the original constructions and the current state of the city center are described and the effect of these changes on the seismic vulnerability of the city is discussed.

Terremoto 2010 en Chile y vivienda social: resultados y aprendizajes para recomendación de políticas públicas

El territorio chileno esta propenso, desde antes que se constituyera como nación, al impacto del comportamiento de la naturaleza que le es inherente y que también le produce daños. Está representado en los seísmos, los más dañinos. Todavía, la sociedad chilena no termina de comprender que esos daños, son parte de un desequilibrio de una convivencia armoniosa entre ella y esa naturaleza, puesto que el ser humano que vive y habita sobre ella, también lo es. Así entonces, cada vez que el territorio y su espacio son remecidos por los seísmos, la naturaleza, manifestada en la sociedad, adquiere nuevos aprendizajes para mejorar la respuesta al próximo evento. El terremoto 2010 de 8.8° Richter, fue el segundo de mayor magnitud después del otro que hasta ahora, es el más grande del planeta, y que pudo ser medido. Aquel, fue el terremoto de Valdivia de 9,5° Richter, ocurrido el 22 de mayo de 1960. Las sociedades no son estáticas, cambian, son dinámicas. Esta vez el seísmo del 2010, ocurrió en una sociedad que hace ya 35 años, adoptó un modelo de economía de libre mercado. La pobreza que tenía a 1990, era de aproximadamente, un 40%. La del 2010, de un 14%. Durante la dictadura militar hubo otro seísmo de 7,8° Richter, recién instalándose el modelo aludido. El del 2010, permite sacar conclusiones en el contexto de este modelo económico. Los resultados aparentemente son interesantes en cuanto a que hubo pocas víctimas pero por otra parte, hubo un gran daño económico. La tesis profundiza en el impacto del seísmo en la dimensión del parque habitacional construido y de la vivienda social y en los habitantes más pobres y vulnerables. Es la primera investigación sobre seísmos y vivienda social en Chile. Se asume la hipótesis que ciertas variables por una parte, y una cultura antisísmica por otra, están presentes y han penetrado en los sectores populares durante los últimos 50 años y que ello, podría estar en la base de los resultados obtenidos. Se plantea una suerte de “matrimonio bien avenido” entre el habitante y políticas públicas en vivienda. De ello, se derivan recomendaciones para mejorar los avances en el problema investigado que se contextualizan en referencia al marco teórico elaborado. Sin embargo, y no obstante lo investigado, lo ya avanzado no garantiza buenos resultados en el próximo evento, Por ello, los aprendizajes nutren a otros, nuevos, que acompañarán a la sociedad chilena en su esencia e identidad como nación. ABSTRACT Long before its establishment as an independent nation, the Chilean territory has been prone to the impact of nature, which is an inherent and damaging feature of this land. Such an impact is represented by earthquakes, which are regarded as the most damaging natural disasters. Today, the Chilean society is still unable to understand that these impacts are part of an unbalanced coexistence between individuals and nature since human beings, who live and inhabit this space, are also an element of nature. Therefore, each time this territory is hit by earthquakes, nature —represented by society— learns new lessons in order to provide a better response to future events. The 2010 earthquake, which rated 8.8 on the Richter scale, was the second largest earthquake after the most powerful earthquake ever recorded. Such an event was the Valdivia earthquake of May 22, 1960, which rated 9.5 on the Richter scale. Societies are not static as they are changing and dynamic. The 2010 earthquake took place within a context in which society operated under a free market economy model that had been running for 35 years. As of 1990, 40 per cent of the population lived in poverty; in 2010, such a figure was reduced to 14 per cent. Likewise, a magnitude 7.8 quake struck the country during the military regime period in the early days of the above model. The 2010 earthquake allows us to draw some conclusions within the context of this economic model. Results are interesting since there were few fatalities but significant economic loss. This thesis provides insights into the impact of the 2010 earthquake on the housing stock, social housing and those living in poverty and vulnerability. This is the first research on earthquakes and social housing conducted in Chile. The hypothesis is that certain variables and anti-seismic culture have permeated popular segments of the population over the last 50 years. The latter may be at the basis of the results obtained during this research. Likewise, this study proposes a certain “happy marriage” between the inhabitant and public policies on housing. The above offers some recommendations intended to further explore this issue; these suggestions are contextualized according to the theoretical framework developed in this research. However, current progress on this matter does not ensure positive results in the event of an earthquake. This is why these lessons will serve as models for future events, which are intrinsically tied to local society and Chilean identity.

A seismic shift? An evaluation of the impact of new media on perceptions of freedom of expression and privacy

New media platforms have changed the media landscape forever, as they have altered our perceptions of the limits of communication, and reception of information. Platforms such as Facebook, Twitter and WhatsApp enable individuals to circumvent the traditional mass media, converging audience and producer to create millions of ‘citizen journalists’. This new breed of journalist uses these platforms as a way of, not only receiving news, but of instantaneously, and often spontaneously, expressing opinions and venting and sharing emotions, thoughts and feelings. They are liberated from cultural and physical restraints, such as time, space and location, and they are not constrained by factors that impact upon the traditional media, such as editorial control, owner or political bias or the pressures of generating commercial revenue. A consequence of the way in which these platforms have become ingrained within our social culture is that habits, conventions and social norms, that were once informal and transitory manifestations of social life, are now infused within their use. What were casual and ephemeral actions and/or acts of expression, such as conversing with friends or colleagues or swapping/displaying pictures, or exchanging thoughts that were once kept private, or maybe shared with a select few, have now become formalised and potentially permanent, on view for the world to see. Incidentally, ‘traditional’ journalists and media outlets are also utilising new media, as it allows them to react, and disseminate news, instantaneously, within a hyper-competitive marketplace. However, in a world where we are saturated, not only by citizen journalists, but by traditional media outlets, offering access to news and opinion twenty-four hours a day, via multiple new media platforms, there is increased pressure to ‘break’ news fast and first. This paper will argue that new media, and the culture and environment it has created, for citizen journalists, traditional journalists and the media generally, has altered our perceptions of the limits and boundaries of freedom of expression dramatically, and that the corollary to this seismic shift is the impact on the notion of privacy and private life. Consequently, this paper will examine what a reasonable expectation of privacy may now mean, in a new media world.

On the use of the exoskeleton for seismic improvement and integrated efficient technologies in existing buildings

The aim of this work is to investigate the seismic improvement obtained through external strengthening structures applied on existing reinforced concrete buildings. An innovative integration with pre-assembled technological envelope components is also presented with the aim of achieving a holistic renovation. Particular attention was paid to the timber solution with an innovative post-tensioned connection between cross-laminated timber panels, which was the subject of an experimental campaign

Up-regulation Of Dnam-1 And Nkp30, Associated With Improvement Of Nk Cells Activation After Long-term Culture Of Mononuclear Cells From Patients With Ovarian Neoplasia.

This study aimed at evaluating the functional activation and activating receptors expression on resting, short- and long-term NK and NK-like T cells from blood of ovarian neoplasia patients. Blood from patients with adnexal benign alterations (n = 10) and ovarian cancer (grade I-IV n = 14) were collected after signed consent. Effector cells activation was evaluated by the expression of the CD107a molecule. Short-term culture was conducted overnight with IL-2 and long-term culture for 21 days, by a method designed to expand CD56(+) lymphocytes. Short-term culture significantly increased NK cells activation compared to resting NK cells (p<0.05), however, the long-term procedure supported an even higher increase (p<0.001). Resting NK-like T cells showed poor activation, which was not altered by the culture procedures. The long-term culture effectively increased the expression of the activating receptors on NK and NK-like T cells, either by increasing the number of cells expressing a given receptor and/or by up-regulating their expression intensity. As a conclusion, the long-term culture system employed, resulted in a high number of functional NK cells. The culture system was particularly efficient on the up-regulation of NKp30 and DNAM-1 receptors on NK cells.

Tissue culture of Cecropia glaziovii Sneth (urticaceae): vegetative micropropagation and plant regeneration from callus

Cecropia glaziovii is a tree with used in Brazilian popular medicine. Methods allowing the clonal propagation of this species are of great interest for superior genotype multiplication and perpetuation. For this reason, we examined the effect of different culture media and different types of explants on adventitious shoot regeneration from callus and buds of C. glaziovii. Leaves, petioles and stipules obtained from aseptically grown seedlings or from pre-sterilized plants were used to initiate cultures. Adventitious shoot regeneration was achieved when apical and axillary buds were inoculated on gelled Murashige & Skoog (MS) medium supplemented with 6-benzylaminopurine alone (BAP) (1.0, 5.0 or 10.0 mg L-1) or combined with -naphthalene acetic acid (NAA) (1.0 or 2.0 mg L-1), after 40 days of culture. Best callus production was obtained after 30 days of petioles' culture on gelled MS medium with 2,4 dichlorophenoxyacetic acid (2,4-D) (5.0 mg L-1) combined with BAP (1.0 mg L-1). Successful shoot regeneration from callus was achieved when MS medium supplemented with zeatin (ZEA) (0.1 mg L-1) alone or combined with 2,4-D (1.0 or 5.0 mg L-1) was inoculated with friable callus obtained from petioles. All shoots were rooted by inoculation on MS medium supplemented with indole-3-acetic acid (IAA) (1.0 mg L-1). Rooted plants transferred to potting soil were successfully established. All in vitro regenerated plantlets showed to be normal, without morphological variations, being also identical to the source plant. Our study has shown that C. glaziovii can be propagated by tissue culture methods, allowing large scale multiplication of superior plants for pharmacological purposes.

Comparison of radial growth rate of the mutualistic fungus of Atta sexdens rubropilosa forel in two culture media

In vitro culture of the mutualistic fungus of leaf-cutting ants is troublesome due to its low growth rate, which leads to storage problems and contaminants accumulation. This paper aims at comparing the radial growth rate of the mutualistic fungus of Atta sexdens rubropilosa Forel in two different culture media (Pagnocca B and MEA LP). Although total MEA LP radial growth was greater all along the bioassay, no significant difference was detected between growth efficiencies of the two media. Previous evidences of low growth rate for this fungus were confirmed. Since these data cannot point greater efficiency of one culture medium over the other, MEA LP medium is indicated for in vitro studies with this mutualistic fungus due its simpler composition and translucent color, making the analysis easier.

Correlation of meta 1 expression with culture stage, cell morphology and infectivity in Leishmania (Leishmania) amazonensis promastigotes

The parasitic protozoan Leishmania (Leishmania) amazonensis alternates between mammalian and insect hosts. In the insect host, the parasites proliferate as procyclic promastigotes andthen differentiate into metacyclic infective forms. The meta 1 gene is preferentially expressed during metacyclogenesis. Meta 1 expression profile determination along parasite growth curves revealed that the meta 1 mRNA level peaked at the early stationary phase then decreased to an intermediate level. No correlation was observed between meta 1 expression and infectivity. Conversely, infectivity correlated with the increase of apoptotic cells in the late stationary phase.

Effect of culture medium on biocalcification by Pseudomonas Putida, Lysinibacillus Sphaericus and Bacillus Subtilis

The objective of this study is to investigate the efficiency of calcium carbonate bioprecipitation by Lysinibacillus sphaericus, Bacillus subtilis and Pseudomonas putida, obtained from the Coleção de Culturas do Instituto Nacional de Controle de Qualidade em Saúde (INCQS), as a first step in determining their potential to protect building materials against water uptake. Two culture media were studied: modified B4 containing calcium acetate and 295 with calcium chloride. Calcium consumption in the two media after incubation with and without the bacterial inoculum was determined by atomic absorption analysis. Modified B4 gave the best results and in this medium Pseudomonas putida INQCS 113 produced the highest calcium carbonate precipitation, followed by Lysinibacillus sphaericus INQCS 414; the lowest precipitation was produced by Bacillus subtilis INQCS 328. In this culture medium XRD analysis showed that Pseudomonas putida and Bacillus subtilis precipitated calcite and vaterite polymorphs while Lysinibacillus sphaericus produced only vaterite. The shape and size of the crystals were affected by culture medium, bacterial strain and culture conditions, static or shaken. In conclusion, of the three strains Pseudomonas putida INQCS 113 in modified B4 medium gave the best results precipitating 96% of the calcium, this strain thus has good potential for use on building materials.

Production of DNA microarray and expression analysis of genes from Xylella fastidiosa in different culture media

DNA Microarray was developed to monitor the expression of many genes from Xylella fastidiosa, allowing the side by-side comparison of two situations in a single experiment. The experiments were performed using X. fastidiosa cells grown in two culture media: BCYE and XDM2. The primers were synthesized, spotted onto glass slides and the array was hybridized against fluorescently labeled cDNAs. The emitted signals were quantified, normalized and the data were statistically analyzed to verify the differentially expressed genes. According to the data, 104 genes were differentially expressed in XDM2 and 30 genes in BCYE media. The present study showed that DNA microarray technique efficiently differentiate the expressed genes under different conditions.