Estudio de la recepción en movimiento de la televisión digital utilizando LDM.

[EN]The purpose of this project is to study and analyse signals of digital television, provided for mobile services, by using LDM technology. In order to achieve this, it will be needed the use of different transmission configurations (modulation, code rate…) suitable for this type of service, as well as propagation channels showing movement situations, such as pedestrian or mobile. This project comes as a response of recent research in LDM technology, which has been proposed as a Physical Layer technology to the ATSC 3.0 Next Generation Digital TV standard.

Estudio de la recepción fija y portátil de la televisión digital mediante el uso de la tecnología LDM.

[Es]En este Trabajo de Fin de Grado se estudia el comportamiento de LDM (Layered Division Multiplexing) para la radiodifusión digital terrestre en los casos de recepción fijo y portátil. Para ello, se adapta un programa que recrea, de la forma más fiel posible, la capa física del sistema LDM. Este programa consta de múltiples parámetros que determinan las características de funcionamiento del sistema. Una vez adaptado, el programa se utiliza para simular el comportamiento del sistema en diferentes entornos de trabajo o canales (Gauss, Rice y Rayleigh) consiguiendo unos resultados que nos permiten evaluar las prestaciones del sistema en cada entorno.

LDM teknologiaren errendimenduaren azterketa multicast zerbitzuetarako

[EN]The purpose of this project is to study the performance and the efficiency of the LDM technology for the transmission of multicast services in a cellular network and vehicular communication environment. In order to achieve this goal, it will be done a comparison with LTE, the technology nowadays used for multicast-grouping techniques. Thus, the specific parameters of each technology are going to be analyzed and a simulation software is going to be developed. Finally, the results from the above mentioned comparison will be shown, as well as an implementation of LDM in multicast-grouping technique.

‘Redstone is like electricity’: Children's performative representations in and around Minecraft

In this article, we investigate eight and nine year old girls’ school and home use of the popular game Minecraft and the ways in which the girls ‘bring themselves into being’ through talk and digital production in the social spaces of the classroom and within the game’s multiplayer online world. This work was conducted as part of a broader digital games in education project involving primary and secondary school-aged students in Australia and focuses specifically on data collected from an all-girls primary school in Brisbane. We investigate the processes of identity construction that occur as the girls undertake practices of curatorship (Potter, 2012) to display their knowledge of Minecraft through discussion of the game, both ‘in world’ and in face-to-face interactions, and as they assemble resources within and around the game to design, build and display their creations and share stories about their game play. The article begins with a consideration of recent scholarship focussing on children, learning and digital culture and literacy practices before explaining how Minecraft is, in many ways, an exemplary instance of a digital game that promotes and enables complex practices of digital participation. We then introduce the concepts of performativity and recognition (Butler 1990, 2004, 2005) which, we argue, provide productive ways to theorise identity work within affinity groups. The article then outlines some background to the research project and our methodology before providing analysis of the data in the second half of the article. We conclude by outlining the implications of our investigation for the conceptualisation of learning spaces as affinity groups and for considering digital participation as curatorship.

Solutions for New Terrestrial Broadcasting Systems Offering Simultaneously Stationary and Mobile Services

221 p.

同步化处理后小鼠成纤维细胞表观遗传水平的相对定量分析

供体细胞的同步化处理可能改变其表观遗传特性,进而影响胚胎的克隆效率。研究同步化处理对小鼠胎儿成纤维细胞(mouse embryonicf ibroblasts,MEFs)组蛋白H3K9甲基化、乙酰化及组蛋白H3K4单甲基化、三甲基化表达的影响。分离培养MEFs,增殖稳定的第3代MEFs分别用5mL/L血清饥饿处理4d或15mL/LDM-SO处理2d使细胞处于增殖抑制期,通过免疫组化染色和Image-J图像处理软件,相对定量比较不同处理情况下组蛋白H3K9甲基化、乙酰化和组蛋白H3K4单甲基化、三甲基化变化情况。Ki-67染色检测结果表明,两种同步化处理可使细胞处于G0期或G1期。DMSO处理使MEFs组蛋白H3K9乙酰化表达水平升高,而5mL/L血清饥饿处理则使其表达水平下降;此外,两种同步化处理均导致组蛋白H3K9甲基化和H3K4单甲基化表达下降,但不影响组蛋白H3K4三甲基化的表达水平。研究结论表明:同步化处理可改变MEFs组蛋白乙酰化和甲基化表达水平,进而有可能影响胚胎克隆效率。

Ligament-derived matrix stimulates a ligamentous phenotype in human adipose-derived stem cells.

Human adipose stem cells (hASCs) can differentiate into a variety of phenotypes. Native extracellular matrix (e.g., demineralized bone matrix or small intestinal submucosa) can influence the growth and differentiation of stem cells. The hypothesis of this study was that a novel ligament-derived matrix (LDM) would enhance expression of a ligamentous phenotype in hASCs compared to collagen gel alone. LDM prepared using phosphate-buffered saline or 0.1% peracetic acid was mixed with collagen gel (COL) and was evaluated for its ability to induce proliferation, differentiation, and extracellular matrix synthesis in hASCs over 28 days in culture at different seeding densities (0, 0.25 x 10(6), 1 x 10(6), or 2 x 10(6) hASC/mL). Biochemical and gene expression data were analyzed using analysis of variance. Fisher's least significant difference test was used to determine differences between treatments following analysis of variance. hASCs in either LDM or COL demonstrated changes in gene expression consistent with ligament development. hASCs cultured with LDM demonstrated more dsDNA content, sulfated-glycosaminoglycan accumulation, and type I and III collagen synthesis, and released more sulfated-glycosaminoglycan and collagen into the medium compared to hASCs in COL (p LDM but not COL constructs (p LDM can stimulate a ligament phenotype by hASCs, and may provide a novel scaffold material for ligament engineering applications.

Sub-produtos de desinfecção da água: síntese e caracterização espectroscópica, implementação de metodologias analíticas e estudo da actividade genotóxica

Dissertação de mest., Engenharia Biológica, Faculdade de Engenharia e de Recursos Naturais, Univ. do Algarve, 2008

Beyond Robotic Wastelands of Time: Abandoned Pedagogical Agents and New Pedalled Pedagogies

Chatbots, known as pedagogical agents in educational settings, have a long history of use, beginning with Alan Turing’s work. Since then online chatbots have become embedded into the fabric of technology. Yet understandings of these technologies are inchoate and often untheorised. Integration of chatbots into educational settings over the past five years suggests an increase in interest in the ways in which chatbots might be adopted and adapted for teaching and learning. This article draws on historical literature and theories that to date have largely been ignored in order to (re)contextualise two studies that used responsive evaluation to examine the use of pedagogical agents in education. Findings suggest that emotional interactions with pedagogical agents are intrinsic to a user’s sense of trust, and that truthfulness, personalisation and emotional engagement are vital when using pedagogical agents to enhance online learning. Such findings need to be considered in the light of ways in which notions of learning are being redefined in the academy and the extent to which new literacies and new technologies are being pedalled as pedagogies in ways that undermine what higher education is, is for, and what learning means.

Développement de méthodes d’analyse directe de polluants organiques volatils à l’état de traces dans l’air et les biogaz

Il est reconnu que le benzène, le toluène, l’éthylbenzène et les isomères du xylène, composés organiques volatils (COVs) communément désignés BTEX, produisent des effets nocifs sur la santé humaine et sur les végétaux dépendamment de la durée et des niveaux d’exposition. Le benzène en particulier est classé cancérogène et une exposition à des concentrations supérieures à 64 g/m3 de benzène peut être fatale en 5–10 minutes. Par conséquent, la mesure en temps réel des BTEX dans l’air ambiant est essentielle pour détecter rapidement un danger associé à leur émission dans l’air et pour estimer les risques potentiels pour les êtres vivants et pour l’environnement. Dans cette thèse, une méthode d’analyse en temps réel des BTEX dans l’air ambiant a été développée et validée. La méthode est basée sur la technique d’échantillonnage direct de l’air couplée avec la spectrométrie de masse en tandem utilisant une source d’ionisation chimique à pression atmosphérique (APCI-MS/MS directe). La validation analytique a démontré la sensibilité (limite de détection LDM 1–2 μg/m3), la précision (coefficient de variation CV < 10%), l’exactitude (exactitude > 95%) et la sélectivité de la méthode. Des échantillons d’air ambiant provenant d’un site d’enfouissement de déchets industriels et de divers garages d’entretien automobile ont été analysés par la méthode développée. La comparaison des résultats avec ceux obtenus par la technique de chromatographie gazeuse on-line couplée avec un détecteur à ionisation de flamme (GC-FID) a donné des résultats similaires. La capacité de la méthode pour l’évaluation rapide des risques potentiels associés à une exposition aux BTEX a été prouvée à travers une étude de terrain avec analyse de risque pour la santé des travailleurs dans trois garages d’entretien automobile et par des expériences sous atmosphères simulées. Les concentrations mesurées dans l’air ambiant des garages étaient de 8,9–25 µg/m3 pour le benzène, 119–1156 µg/m3 pour le toluène, 9–70 µg/m3 pour l’éthylbenzène et 45–347 µg/m3 pour les xylènes. Une dose quotidienne environnementale totale entre 1,46 10-3 et 2,52 10-3 mg/kg/jour a été déterminée pour le benzène. Le risque de cancer lié à l’exposition environnementale totale au benzène estimé pour les travailleurs étudiés se situait entre 1,1 10-5 et 1,8 10-5. Une nouvelle méthode APCI-MS/MS a été également développée et validée pour l’analyse directe de l’octaméthylcyclotétrasiloxane (D4) et le décaméthylcyclopentasiloxane (D5) dans l’air et les biogaz. Le D4 et le D5 sont des siloxanes cycliques volatils largement utilisés comme solvants dans les processus industriels et les produits de consommation à la place des COVs précurseurs d’ozone troposphérique tels que les BTEX. Leur présence ubiquitaire dans les échantillons d’air ambiant, due à l’utilisation massive, suscite un besoin d’études de toxicité. De telles études requièrent des analyses qualitatives et quantitatives de traces de ces composés. Par ailleurs, la présence de traces de ces substances dans un biogaz entrave son utilisation comme source d’énergie renouvelable en causant des dommages coûteux à l’équipement. L’analyse des siloxanes dans un biogaz s’avère donc essentielle pour déterminer si le biogaz nécessite une purification avant son utilisation pour la production d’énergie. La méthode développée dans cette étude possède une bonne sensibilité (LDM 4–6 μg/m3), une bonne précision (CV < 10%), une bonne exactitude (> 93%) et une grande sélectivité. Il a été également démontré qu’en utilisant cette méthode avec l’hexaméthyl-d18-disiloxane comme étalon interne, la détection et la quantification du D4 et du D5 dans des échantillons réels de biogaz peuvent être accomplies avec une meilleure sensibilité (LDM ~ 2 μg/m3), une grande précision (CV < 5%) et une grande exactitude (> 97%). Une variété d’échantillons de biogaz prélevés au site d’enfouissement sanitaire du Complexe Environnemental de Saint-Michel à Montréal a été analysée avec succès par cette nouvelle méthode. Les concentrations mesurées étaient de 131–1275 µg/m3 pour le D4 et 250–6226 µg/m3 pour le D5. Ces résultats représentent les premières données rapportées dans la littérature sur la concentration des siloxanes D4 et D5 dans les biogaz d’enfouissement en fonction de l’âge des déchets.

Diseño y Evaluación de intervenciones en organizaciones empresariales desde la dinámica de sistemas – revisión teórica. Sistemas – Revisión Teórica.

Este trabajo tiene como objetivo hacer una revisión teórica desde los inicios de la Teoría General de Sistemas, hasta el desarrollo de la dinámica de sistemas, todo lo que estas dos teorías plantean, además de muchas otras teorías de las cuales estas se encuentran rodeadas y conectadas muy cercanamente, ya que partiendo del planteamiento que hizo el biólogo Ludwig Von Bertalanffy se derivaron diversas corrientes de pensamiento, todas alrededor del enfoque y pensamiento de sistemas, y de la cual se deriva la más importante para nosotros en este trabajo de la cual es padre el Ingeniero Jay Wright Forrester, la Dinámica de Sistemas. Muchas de estas otras teorías tienen como objetivo generar estrategias de cambio en las organizaciones mediante diferentes metodologías, pero todas con el fin de alcanzar un desempeño óptimo en los procesos que son realizados en las organizaciones. Este proyecto es planteado ante la necesidad de tener un amplio conocimiento teórico de todo lo que conllevan los planteamientos de la Dinámica de Sistemas los cuales van de la mano junto con el pensamiento sistémico y el modelamiento organizacional, el cual veremos cómo es desarrollado desde el enfoque de la Dinámica de Sistemas a través de distintos modelos y en la actualidad herramientas informáticas tales como software diseñados exclusivamente para el modelamiento desde la Dinámica de Sistemas.

Efficacy of dip slide test for mutans streptococci in caries risk assessment

The aim of this study was to evaluate the efficacy of a dip slide test for mutans streptococci in caries risk assessment, when the microbiological results were compared to well-defined clinical criteria (DCC) for caries risk, clinically measured through high and low caries activity. Eighty-one volunteers from the 6(th) to 8(th) grades from public schools of Piracicaba, São Paulo, Brazil, were evaluated for dental caries. All free smooth surfaces were evaluated to check whether or not there were white spots. Based on the subjects' caries experience, a calibrated clinician divided them into groups of high and low caries activity. The subjects were submitted to a salivary test (CARITEST SM (R)) from the same batch number. Kappa statistics (kappa) were applied to verify the reproducibility of the simplified test, checked through interexaminer agreement when the results were classified by independent and blind means. The microbiological results were validated according to expressions of sensitivity and specificity. A moderate agreement was verified as the results were classified according to 6 scores (kappa =0.55), and the agreement was substantial when the results were classified according to high and low microbiological count (kappa =0.78). The sensitivity and specificity values were 0.59 and 0.85, respectively, showing that the test was more specific than sensitive, and could thus better identify the low caries risk subjects.

Aspectos produtivos do capim-elefante (Pennisetum purpureum, Schum.) cv. Roxo no brejo paraibano

O trabalho foi realizado com o objetivo de verificar o efeito de diferentes alturas de corte sobre a produtividade do capim-elefante cv. Roxo em épocas de seca e chuva no Brejo paraibano. O esquema experimental foi um fatorial 4 x 2, sendo quatro alturas de corte (0, 15, 30 e 45 cm), duas épocas (períodos seco e chuvoso) e quatro blocos. Foram avaliadas as produções por hectare de massa verde (MV), matéria seca total (MS), de folhas (MSF) e colmos (MSC) e proteína bruta (PB). Após o corte de uniformização, efetuaram-se dois cortes no período seco em intervalos de 90 dias e três no período chuvoso em intervalos de 60 dias. Não houve interação entre altura de corte e a época. Entretanto, à medida que se elevou a altura do corte, reduziram-se as produções de MV, MS e MSC. As produções de PB e MSF não diferiram. Quando elevadas as alturas dos cortes de 0 para 45 cm, houve redução de aproximadamente 33% na produção para MV, 24,83% para MSF e 60% para MSC. Os cortes no período seco foram mais produtivos em relação aos da época chuvosa. As médias das produções de MS, MV, MSF, MSC e PB foram, respectivamente, 4,12; 21,19; 2,65; 1,47 e 0,32 t/ha no período seco e 12,44; 2,45; 1,81; 0,54 e 0,17 t/ha no período chuvoso.

Acúmulo e exportação de nutrientes em cenoura 'Forto'

In order to quantify the growth, accumulation and exportation of macronutrients by carrot 'Forto', and obtain equations that best represent them, it was conducted an experiment in the São Gotardo (MG), from May to September 2004. Samples were taken at 40, 50, 60, 70, 80, 90, 100, 110 and 120 days after sowing (DAS) to determine dry matter and accumulation of macronutrients in leaves and roots. At 40 DAS, five days after thinning, the plants had 0.18 g and 0.04 g in dry leaves (LDM) and of roots (RDM), respectively. From then, until 88 DAS, the partitioning of photoassimilates and accumulation of matter in the shoots were more pronounced. The RDM was low until 80 DAS, which corresponded to two thirds of the crop cycle, from when the amount of material allocated in this part of the plant had strong growth, surpassing, at 88 DAS, the amount of LDM. The nutrient accumulation was small in the first 60 DAS, coinciding with the period of lower dry matter accumulation. The order of decreasing macronutrient accumulation by the crop were: K > N > Ca > P > S > Mg, in the amounts of 906.7, 438, 155.46, 87.4, 58 and 37.63 mg plant-1, respectively. The root participated with 60.5% of the accumulation of N, 86.1% of P, 58% of K, 25.5% of Ca, 55.6% of Mg and 65.5% of S. Considering a population of 590,000 plants per hectare, the total quantity of nutrients by the crop of carrots was 258.3, 51.6, 534.8, 91.7, 22.2 and 34.2 kg N ha-1, P, K, Ca, Mg and S, respectively.

Crescimento e acúmulo de macronutrientes em cenoura Forto‟

Pós-graduação em Agronomia (Ciência do Solo) - FCAV