Kuzure Kami Shiho Gatame

Vídeo demostrativo de la técnica de Judo en suelo Inmovilización Kuzure Kami Shiho Gatame, Variante del control superior sobre cuatro puntos o Broken Upper Four-Quarter Hold Down.

Nanostructured silver-gold bimetallic SERS substrates for selective identification of bacteria in human blood

Surface-enhanced Raman spectroscopy (SERS) is a potentially important tool in the rapid and accurate detection of pathogenic bacteria in biological fluids. However, for diagnostic application of this technique, it is necessary to develop a highly sensitive, stable, biocompatible and reproducible SERS-active substrate. In this work, we have developed a silver–gold bimetallic SERS surface by a simple potentiostatic electrodeposition of a thin gold layer on an electrochemically roughened nanoscopic silver substrate. The resultant substrate was very stable under atmospheric conditions and exhibited the strong Raman enhancement with the high reproducibility of the recorded SERS spectra of bacteria (E. coli, S. enterica, S. epidermidis, and B. megaterium). The coating of the antibiotic over the SERS substrate selectively captured bacteria from blood samples and also increased the Raman signal in contrast to the bare surface. Finally, we have utilized the antibiotic-coated hybrid surface to selectively identify different pathogenic bacteria, namely E. coli, S. enterica and S. epidermidis from blood samples.

Towards improved precision in the quantification of surface-enhanced Raman scattering (SERS) enhancement factors: A renewed approach

This paper demonstrates a renewed procedure for the quantification of surface-enhanced Raman scattering (SERS) enhancement factors with improved precision. The principle of this method relies on deducting the resonance Raman scattering (RRS) contribution from surface-enhanced resonance Raman scattering (SERRS) to end up with the surface enhancement (SERS) effect alone. We employed 1,8,15,22-tetraaminophthalocyanato-cobalt(II) (4α-CoIITAPc), a resonance Raman- and electrochemically redox-active chromophore, as a probe molecule for RRS and SERRS experiments. The number of 4α-CoIITAPc molecules contributing to RRS and SERRS phenomena on plasmon inactive glassy carbon (GC) and plasmon active GC/Au surfaces, respectively, has been precisely estimated by cyclic voltammetry experiments. Furthermore, the SERS substrate enhancement factor (SSEF) quantified by our approach is compared with the traditionally employed methods. We also demonstrate that the present approach of SSEF quantification can be applied for any kind of different SERS substrates by choosing an appropriate laser line and probe molecule.

Electrochemical pathway for the quantification of SERS enhancement factor

This communication presents a new pathway for the more precise quantification of surface-enhanced Raman scattering (SERS) enhancement factor via deducing resonance Raman scattering (RRS) effect from surface-enhanced resonance Raman scattering (SERRS). To achieve this, a self-assembled monolayer of 1,8,15,22-tetraaminophthalocyanatocobalt(II) (4α-CoIITAPc) is formed on plasmon inactive glassy carbon (GC) and plasmon active GC/AuNPs surface. The surfaces are subsequently used as common probes for electrochemical and Raman (RRS and SERRS) studies. The most crucial parameters required for the quantification of SERS substrate enhancement factor (SSEF) such as real surface area of GC/AuNPs substarte and the number of 4α-CoIITAPc molecules contributing to RRS (on GC) and SERRS (on GC/AuNPs) are precisely estimated by cyclic voltammetry experiments. The present approach of SSEF quantification can be applied to varieties of surfaces by choosing an appropriate laser line and probe molecule for each surface.

Detection of DNA mutations using novel SERS (Surface-Enhanced Raman Spectroscopy) diagnostic platform

This article describes the detection of DNA mutations using novel Au-Ag coated GaN substrate as SERS (surface-enhanced Raman spectroscopy) diagnostic platform. Oligonucleotide sequences corresponding to the BCR-ABL (breakpoint cluster region-Abelson) gene responsible for development of chronic myelogenous leukemia were used as a model system to demonstrate the discrimination between the wild type and Met244Val mutations. The thiolated ssDNA (single-strand DNA) was immobilized on the SERS-active surface and then hybridized to a labeled target sequence from solution. An intense SERS signal of the reporter molecule MGITC was detected from the complementary target due to formation of double helix. The SERS signal was either not observed, or decreased dramatically for a negative control sample consisting of labeled DNA that was not complementary to the DNA probe. The results indicate that our SERS substrate offers an opportunity for the development of novel diagnostic assays.

Rozproszone algorytmy aproksymacyjne dla grafów dyskowych

Wydział Matematyki i Informatyki: Zakład Matematyki Dyskretnej

PHYTOCHROME KINASE SUBSTRATE4 modulates phytochrome-mediated control of hypocotyl growth orientation

Gravity and light are major factors shaping plant growth. Light perceived by phytochromes leads to seedling deetiolation, which includes the deviation from vertical hypocotyl growth and promotes hypocotyl phototropism. These light responses enhance survival of young seedlings during their emergence from the soil. The PHYTOCHROME KINASE SUBSTRATE (PKS) family is composed of four members in Arabidopsis (Arabidopsis thaliana): PKS1 to PKS4. Here we show that PKS4 is a negative regulator of both phytochrome A- and B-mediated inhibition of hypocotyl growth and promotion of cotyledon unfolding. Most prominently, pks4 mutants show abnormal phytochrome-modulated hypocotyl growth orientation. In dark-grown seedlings hypocotyls change from the original orientation defined by seed position to the upright orientation defined by gravity and light reduces the magnitude of this shift. In older seedlings with the hypocotyls already oriented by gravity, light promotes the deviation from vertical orientation. Based on the characterization of pks4 mutants we propose that PKS4 inhibits changes in growth orientation under red or far-red light. Our data suggest that in these light conditions PKS4 acts as an inhibitor of asymmetric growth. This hypothesis is supported by the phenotype of PKS4 overexpressers. Together with previous findings, these results indicate that the PKS family plays important functions during light-regulated tropic growth responses

PHYTOCHROME KINASE SUBSTRATE 1 is a phototropin 1 binding protein required for phototropism.

Phototropism, or plant growth in response to unidirectional light, is an adaptive response of crucial importance. Lateral differences in low fluence rates of blue light are detected by phototropin 1 (phot1) in Arabidopsis. Only NONPHOTOTROPIC HYPOCOTYL 3 (NPH3) and root phototropism 2, both belonging to the same family of proteins, have been previously identified as phototropin-interacting signal transducers involved in phototropism. PHYTOCHROME KINASE SUBSTRATE (PKS) 1 and PKS2 are two phytochrome signaling components belonging to a small gene family in Arabidopsis (PKS1-PKS4). The strong enhancement of PKS1 expression by blue light and its light induction in the elongation zone of the hypocotyl prompted us to study the function of this gene family during phototropism. Photobiological experiments show that the PKS proteins are critical for hypocotyl phototropism. Furthermore, PKS1 interacts with phot1 and NPH3 in vivo at the plasma membrane and in vitro, indicating that the PKS proteins may function directly with phot1 and NPH3 to mediate phototropism. The phytochromes are known to influence phototropism but the mechanism involved is still unclear. We show that PKS1 induction by a pulse of blue light is phytochrome A-dependent, suggesting that the PKS proteins may provide a molecular link between these two photoreceptor families.

Estilos intelectuales de aprendizaje en la interacción educativa de profesores-as, estudiantes : hacia una enseñanza adaptativa.

Identificar los estilos intelectuales y los estilos de enseñanza y aprendizaje de profesores y alumnos y examinar las relaciones entre ellos.. Muestra 1: 459 alumnos-as de ESO (40 por ciento) y primero de Bachillerato (60 por ciento) de tres institutos de Enseñanza Secundaria de Murcia y Cartagena. Muestra 2: 108 sujetos que imparten docencia a los alumnos anteriores.. Se realiza primero un estudio teórico sobre el origen, evolución y tratamiento del concepto de estilos intelectuales desde diferentes disciplinas. Se revisan conceptualmente los términos estilo de aprendizaje y enseñanza y se analizan los estilos intelectuales propuestos por Sternberg para referirse al autogobierno mental del alumno y del profesor. Después se realiza un estudio empírico para examinar la relación del estilo intelectual y de aprendizaje de los estudiantes con el estilo intelectual y de instrucción del profesor. Se adaptan los instrumentos, se aplican a la muestra de estudiantes y profesores y se recogen las calificaciones finales de los alumnos. Se realizan análisis correlacionales entre las variables de los distintos estilos intelectuales, análisis de varianza y pruebas t de diferencia de medias para examinar las diferencias entre los centros y se calcula el índice de similitud para la comparación de perfiles de profesores-alumnos en cada asignatura.. Test de Factor G de Catell de nivel 3, Inventario de Estilos Intelectuales para Alumnos de Sternberg, Cuestionario de Tareas Abiertas para la Medida del Estilo Intelectual de Sternberg, Inventario de Estilos Intelectuales de los Alumnos Evaluados por el Profesor de Sternberg, Knowledge Accessing Modes Inventory (KAMI) de Rancourt, Indicador de Tipo Myers-Briggs Forma G, NASSP, Learning Styles Inventory (LSI) de Canfield, Inventario de Estilos Intelectuales para Profesores de Sternberg, Inventario de Estilos Instruccionales (ISI) de Canfield.. Los resultados de la adaptación de los diferentes instrumentos son satisfactorios, mostrando unos índices de fiabilidad adecuados. Se evidencian bastantes relaciones significativas entre los diversos estilos intelectuales y de aprendizaje del alumno entre sí y con las calificaciones escolares. Se revelan diferencias entre los tres centros estudiados en el estilo intelectual de los alumnos, así como en varios estilos de aprendizaje, aunque no ocurre lo mismo cuando se tienen en cuenta los estilos intelectuales y de enseñanza del profesorado. La comparación de perfiles de profesores y alumnos pone de manifiesto que los profesores de las distintas asignaturas muestran estilos característicos predominantes y que, en algunos casos, la coincidencia entre el estilo intelectual del profesor y el del alumno está asociada a las calificaciones obtenidas por éste. Esto parece indicar que existe una mayor relación entre los estilos intelectuales de los alumnos y sus calificaciones cuando coinciden el estilo intelectual del alumno y el del profesor..

Katalizatory złotowo-miedziowe na nośnikach cerowych, cyrkonowych i cerowo-cyrkonowych – preparatyka, charakterystyka i zastosowanie w procesach utleniania

Wydział Chemii

Why it is hard to fight against cyber criminals?

We are witnessing numerous cyber attacks every day, however, we do not see many cyber criminals are brought to justice. One reason is that it is technically hard to identify and trace cyber criminals. One reason for this passive situation is our limited or even inappropriate understanding of the cyber space. In this paper, we survey the challenges and opportunities in this research field for interested readers. We also list promising tools and directions based on our understanding.

Distributed denial of service (DDoS) detection by traffic pattern analysis

In this paper, we propose a behavior-based detection that can discriminate Distributed Denial of Service (DDoS) attack traffic from legitimated traffic regardless to various types of the attack packets and methods. Current DDoS attacks are carried out by attack tools, worms and botnets using different packet-transmission rates and packet forms to beat defense systems. These various attack strategies lead to defense systems requiring various detection methods in order to identify the attacks. Moreover, DDoS attacks can craft the traffics like flash crowd events and fly under the radar through the victim. We notice that DDoS attacks have features of repeatable patterns which are different from legitimate flash crowd traffics. In this paper, we propose a comparable detection methods based on the Pearson’s correlation coefficient. Our methods can extract the repeatable features from the packet arrivals in the DDoS traffics but not in flash crowd traffics. The extensive simulations were tested for the optimization of the detection methods. We then performed experiments with several datasets and our results affirm that the proposed methods can differentiate DDoS attacks from legitimate traffics.

Colapsos financeiros no Brasil

Nosso trabalho pretendeu servir apenas como um subsídio, ou um ponto de partida, para estudos mais amplos e mais completos. O assunto é muito vasto e pode ser abordado por vários ângulos e muitos matizes. Acreditamos que numa década de tempos turbulentos, os instrumentos de controle dos colapsos devem ser mais eficazes para a defesa do interesse público. Apresentação das Legislações Brasileiras, Inglesa e Americana. Resumo teórico da Literatura Financeira sobre colapsos financeiros. Amostra de concordatas com passivo acima de Cr$ 100 milhões no período 1973/79. Estudo de casos: Siam Util, Construtora Adolpho Lindenberg e Bergamo Companhia Industrial. O objetivo desta monografia é propor um amplo debate ao nível acadêmico (Faculdades de Direito, Administração de Empresas, Ciências Contabeis e Econômicas), empresarial, e político para a promulgação duma NOVA LEI DE REORGANIZAÇÃO PARA A EMPRESA PRIVADA.