Dissemination of bla(IMP-1)-carrying integron In86 among Klebsiella pneumoniae isolates harboring a new trimethoprim resistance gene dfr23

The genetic context of the bla(IMP-1) gene was evaluated in 9 Klebsiella pneumoniae isolates recovered from 2 hospitals in Sao Paulo, Brazil. All isolates harbored a copy of In86 carrying bla(Imp-1), aac(6`)-31, and aadAl. Eight strains from the same hospital also carried another class I integron harboring a new trimethoprim resistance gene (dfr23) that was chromosomally embedded. In86 was likely to be in a 30-kb nontransferable plasmid and was flanked upstream by a sequence identical to one identified in an IMP-1-producing Pseudomonas putida isolate. The bla(IMP-1)-carrying integron In86 was recently reported from nonfermentative bacilli isolated in Sao Paulo. These isolates appear to be the Source of this integron now acquired by K. pneumoniae strains from different hospitals in the same city. Metallo-beta-lactamase production is still rare among Enterobacteriaceae isolates in Brazil, but the acquisition of genetic structures carrying these mobile resistance determinants is worrisome and could lead to an increase in the prevalence of these phenotypes of resistance. (C) 2008 Elsevier Inc. All rights reserved.

Crystal structures of free, IMP-, and GMP-bound Escherichia coli hypoxanthine phosphoribosyltransferase

Crystal structures have been determined for free Escherichia coli hypoxanthine phosphoribosyltransferase (HPRT) (2.9 Angstrom resolution) and for the enzyme in complex with the reaction products, inosine 5'-monophosphate (IMP) and guanosine 5-monophosphate (GMP) (2.8 Angstrom resolution). Of the known 6-oxopurine phosphoribosyltransferase (PRTase) structures, E. coli HPRT is most similar in structure to that of Tritrichomonas foetus HGXPRT, with a rmsd for 150 Calpha atoms of 1.0 Angstrom. Comparison of the free and product bound structures shows that the side chain of Phe156 and the polypeptide backbone in this vicinity move to bind IMP or GMP. A nonproline cis peptide bond, also found in some other 6-oxopurine PRTases, is observed between Leu46 and Arg47 in both the free and complexed structures. For catalysis to occur, the 6-oxopurine PRTases have a requirement for divalent metal ion, Usually Mg2+ in vivo. In the free structure, a Mg2+, is coordinated to the side chains of Glu103 and Asp104. This interaction may be important for stabilization of the enzyme before catalysis. E. coli HPRT is unique among the known 6-oxopurine PRTases in that it exhibits a marked preference for hypoxanthine as substrate over both xanthine and guanine. The structures suggest that its substrate specificity is due to the modes of binding of the bases. In E. coli HPRT, the carbonyl oxygen of Asp 163 would likely form a hydrogen bond with the 2-exocyclic nitrogen of guanine (in the HPRT-guanine-PRib-PP-Mg2+ complex). However, hypoxanthine does not have a 2-exocyclic atom and the HPRT-IMP structure suggests that hypoxanthine is likely to occupy a different position in the purine-binding pocket.

Caminhamos para o imp??sto ??nico?

Sentimos de antem??o o sorriso altamente ir??nico que o leitor pespegar?? nos l??bios ao receber a pergunta em ep??grafe. Ora, pensar??, mas ent??o isso ?? pergunta que se fa??a?! N??o est?? a?? o exemplo de Henry George, relegado ao esc??rnio dos s??culos precisamente porque teve a ???genial??? id??ia de pregar durante sua vida de cientista o imp??sto ??nico s??bre o rendimento da terra?! Se retrucarmos que vamos tratar apenas do imp??sto s??bre a renda, ainda nos lembrar??, prestimoso: n??o est?? a?? o exemplo mais recente de Bartolomeo Benvenuti, que tem recebido apupos de t??da a It??lia, porque em seu II Sistema dei Tributi pregou nada mais nada menos que o imp??sto ??nico s??bre a renda?! E, al??m do mais ??? ajuntar?? ainda o leitor intransigente ??? Seligman, o corifeu non plus ultra de tal mat??ria, j?? n??o situou inflexivelmente a quest??o, esclarecendo terminantemente no final de seu artigo inserto na Encyclopaedia of the Social Sciences ??? com ??nfase de quem encerra vez por t??das uma querela ??? que ???o imp??sto s??bre a renda n??o pode ser mais que um dos elementos de um sistema tribut??rio plural????

Extending the reservoir of bla IMP-5: the emerging pathogen Acinetobacter bereziniae

Acinetobacter bereziniae clinical relevance is starting to be recognized; however, very few descriptions of its carbapenem resistance currently exist. Here we characterize two carbapenem-resistant A. bereziniae isolates. Materials & methods: Isolates were obtained from environmental and clinical samples. Carbapenemases were searched by phenotypic, biochemical and PCR assays. Clonality was studied by ApaI-PFGE and genetic location for carbapenemase genes were assessed by I-CeuI and S1 hybridizations. Results: Isolates were not clonally related but both produced the exclusively Portuguese IMP-5, with the clinical isolate also producing an OXA-58. The carbapenemase genes were plasmid located. Conclusion: Our results emphasize the role of non-baumannii Acinetobacter species as important reservoirs of clinically relevant resistance genes that could also contribute to their emergence as nosocomial pathogens

Procedimiento estandarizado de operación: Método de determinación del consumo de oxígeno en invertebrados acuáticos (PEO-IMP-CO-001)

Expone el procedimiento estándar de operación (PEO) para el método de determinación del consumo de oxígeno de invertebrados acuáticos, aplicable por los analistas de la Línea de Investigación en Ecotoxicología Acuática (LIEA). Describe el equipamiento, materiales necesarios, procedimientos de campo y laboratorio, y análisis e interpretación de los datos.

Procedimiento estandarizado de operación: Método de determinación de la tasa de ingestión de organismos filtradores (PEO-IMP-TI-001)

Presenta el procedimiento estándar de operación (PEO) para el método de determinación de la tasa de ingestión de organismos acuáticos filtradores. Describe el equipamiento, materiales necesarios, procedimientos de campo y laboratorio y análisis e interpretación de los datos.

Imp. Caes. Carolo VI Augusto pio victori gentis suae sideri felicissimo chorographiam hanc exhibentem primar. regionum Habspurgicar. situm positumq. ... pietatis supplex dicat provincia Brisgoia.

Irtokartta

Análise de inosina-5'-monofosfato (5'-IMP) e seus derivados em diferentes músculos de bovinos, suínos e ovinos

As concentrações de inosina-5'-monofosfato e seus derivados (adenosina monofosfato, hipoxantina e inosina) nos músculos Psoas major, Latissimus dorsi, Tensor fascia e Extensor carpi radialis de bovinos, suínos e ovinos foram determinadas através de Cromatografia Líquida de Alta Eficiência (CLAE). A Análise de Variância (ANOVA) dos dados mostraram que as concentrações de 5'-IMP e seus derivados foram significativamente diferentes entre as três espécies (p < 0,001). A maior concentração de 5'-IMP foi detectada nos músculos de ovinos (média de 108,2 mg/100g) seguida pelos de suínos (média de 97 mg/100g) e pelos de bovinos (média de 69,3 mg/100g). Houve variações significativas nas concentrações no músculo, entre os animais nas espécies bovina (p < 0,01) e ovina (p < 0,05), porém não entre os da espécie suína (p > 0,05). Não houve diferenças significativas entre os tipos de músculo em bovinos e ovinos (p > 0,05), mas em suínos (p < 0,01) observou-se variação significativa.

SENSORY ANALYSIS OF A MODEL SYSTEM USING 5'-IMP AND CYSTEINE AT DIFFERENT pH

Sensory analysis was used to get an overall flavour description of a reaction mixtures containing 5'-IMP and Cysteine. Ribose/cysteine systems were used as reference systems. Results from triangle and aroma profiling show a clear correlation between the terms used and the volatile analysis described in literature for these model systems. For instance reactions at pH 3.0 and 4.5 for 5'-IMP/cysteine systems, which were described as "meaty" and "boiled meat" by panellists, presented, in the literature, the higher number of "meaty" compounds in volatile analysis (1, 7, 8, 20) .

Catalogus librorum & instrumentorum chirurgicorum b. m. viri Josephi Pippingsköld, m. d. profess. chirurg. [et] art. obstetr. ad Imp. Acad. Aboëns. nec non imp. coll. med. assessoris, sub hasta d. Oct. XXX & sqq. a. MDCCCXVI

Julkaisuvuosi nimekkeestä.

Evsebii Pamphili episcopi Caesariensis Opera, in duos diuisa tomos,omnia, que hactenus inueniri potuerunt, nuc denuo ex uetustiss. Grecisexemplaribus, aucta & correcta in ordinemq redacta. In hac editione accessit Panegyricus de laudibus Constantini Magni, Imp. Augusti, continens multa quae apud alios historicos uix inueniuntur

UANL

Detection of SPM and IMP metallo-beta-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital

Phenotypic and genotypic SPM and IMP metallo-beta-lactamases (MBL) detection and also the determination of minimal inhibitory concentrations (MIC) to imipenem, meropenem and ceftazidime were evaluated in 47 multidrug-resistant Pseudomonas aeruginosa isolates from clinical specimens. Polymerase chain reaction detected 14 positive samples to either bla(SPM) or bla(IMP) genes, while the best phenotypic assay (ceftazidime substrate and mercaptopropionic acid inhibitor) detected 13 of these samples. Imipenem, meropenem and ceftazidime MICs were higher for MBL positive compared to MBL negative isolates. We describe here the SPM and IMP MBL findings in clinical specimens of P. aeruginosa from the University Hospital of Botucatu Medical School, São Paulo, Brazil, that reinforce local studies showing the high spreading of bla(SPM) and bla(IMP) genes among Brazilian clinical isolates.