Desquamation Is A Novel Phenomenon For Collective Prostate Epithelial Cell Deletion After Castration.

The mechanism underlying castration-induced prostate regression, which is a classical physiological concept translated into the therapeutic treatment of advanced prostate cancer, involves epithelial cell apoptosis. In searching for events and mechanisms contributing to prostate regression in response to androgen modulation, we have frequently observed the collective deletion of epithelial cells. This work was undertaken to characterize this phenomenon hereafter named desquamation and to verify its presence after 17β-estradiol (E2) administration. Electron microscopy revealed that the desquamating cells had preserved cell-cell junctions and collapsed nuclear contents. The TUNEL reaction was negative for these cells, which were also negative for cleaved caspases-8, -9, -3 and nuclear apoptosis-inducing factor. Detailed analyses revealed that the condensed chromatin was first affected detaching from the nuclear lamina, which was observable after lamin A immunohistochemistry, suggesting the lack of lamin A degradation. A search in animals treated with supraphysiological E2 employed as an alternative anti-androgen treatment revealed no desquamation. The combined treatment (Cas + E2 group) caused changes particular to each treatment, including desquamation. In conclusion, desquamation appeared as a novel phenomenon contributing to collective prostate epithelial cell deletion, distinct from the classical castration-induced apoptosis and particular to the androgen deprivation resulting from surgical castration, and should be considered as part of the mechanisms promoting organ regression.

Deposits of transforming growth factor-beta-induced protein in granular corneal dystrophy type II after LASIK.

PURPOSE: To analyze components of the deposits in the corneal flap interface of granular corneal dystrophy type II (GCD II) patients after laser in situ keratomileusis (LASIK). METHODS: Four corneal GCD II specimens displaying disease exacerbation after LASIK were analyzed. Three of these specimens included the recipient corneal button after penetrating keratoplasty or deep lamellar keratoplasty for advanced GCD II after LASIK. The fourth specimen, a similar case of GCD II after LASIK, included the amputated corneal flap. Specimens were processed for histopathologic and immunohistochemical analyses. RESULTS: Corneal stromal deposits in the LASIK flaps of all specimens were stained with 3 anti-transforming growth factor-beta-induced protein (TGFBIp) antibodies. The deposits displayed bright red color staining with Masson trichrome; however, negative staining was seen with Congo red, suggesting that hyaline is the main component localizing to the TGFBIp deposits rather than amyloid. CONCLUSIONS: Amorphous granular material deposited along the interface of the LASIK flap in GCD II corneas is composed mainly of hyaline deposits.

Homogenous implant in rat tibias of matrix preserved in 98% glycerin: histomorphologic study.

As a chemical medium for preservation of tissues, glycerin has shown good results because it maintains the cellular integrity despite the tissue dehydration it causes. Taking advantage of the osteoinducing properties of the osseous matrix and glycerin as a proper medium for tissue preservation, osseous matrix was implanted in rat tibias. Twenty-four rats were used, each receiving two surgical wounds. In one of the wounds an osseous matrix preserved in 98% glycerin was implanted and the other received a matrix without preservatives. Six animals were sacrificed on days 10, 20, 30 and 60 post-implant. After routine histological processing, the specimens were stained in hematoxylin-eosin and Masson's trichrome. The results showed that the matrixes preserved in glycerin presented faster resorption with replacement by newly formed tissue.

Morphology of collagen fibers and elastic system fibers in actinic cheilitis

Background: Actinic cheilitis (AC) is a premalignant condition intimately related to exposure of the lips to sun rays. Aim: The objective of this study was to evaluate the elastic and collagen fibers in the lamina propria of AC. The degree of epithelial atypia was correlated with the quantity of elastic and collagen fibers. Materials and Methods: Fifty-one cases were investigated. One slide was stained with hematoxylin-eosin for the evaluation of atypia, the second was stained with Weigert′s resorcin-fuchsin for the assessment of elastic fibers, and the third slide was stained with Mallory′s trichrome for the analysis of collagen fibers. Results: Ordinal logistic regression analysis revealed a significant correlation between the presence of atypia and collagen fibers (P<0.05). Conclusions: It was concluded that there seems to be a reduction in the quantity of collagen fibers in cases of moderate and severe atypia. No correlation was observed between the degradation of elastic system fibers and the grade of dysplasia.

Xylitol production from DEO hydrolysate of corn stover by Pichia stipitis YS-30

Corn stover that had been treated with vapor-phase diethyl oxalate released a mixture of mono- and oligosaccharides consisting mainly of xylose and glucose. Following overliming and neutralization, a d-xylulokinase mutant of Pichia stipitis, FPL-YS30 (xyl3-a dagger 1), converted the stover hydrolysate into xylitol. This research examined the effects of phosphoric or gluconic acids used for neutralization and urea or ammonium sulfate used as nitrogen sources. Phosphoric acid improved color and removal of phenolic compounds. d-Gluconic acid enhanced cell growth. Ammonium sulfate increased cell yield and maximum specific cell growth rate independently of the acid used for neutralization. The highest xylitol yield (0.61 g(xylitol)/g(xylose)) and volumetric productivity (0.18 g(xylitol)/g(xylose) l) were obtained in hydrolysate neutralized with phosphoric acid. However, when urea was the nitrogen source the cell yield was less than half of that obtained with ammonium sulfate.

Iggeret haq-qôdeš le-rabbî Yôsēf Sôfēr m-Îrûšālayim : dos izṭ der heiliger brif

fun ... (Yôsēf Sôfēr) ...

Mekîrat Yôsēf

[Baermann von Limburg]

Maḥazē mekîrat Yôsēf : ein ṭeaṭer šṭik in 7 akṭin un in 17 szenen ; giformirṭ noch di besṭe un gleichsṭe deos fun gemore ...

fun Eljakim Tzunzer

Le roi d'Ys : légende bretonne : opéra en 3 actes & 5 tableaux /

Reissued from Hartmann plates. Pl. no.: G.H. & Cie. 1745.

Omstandig journaal van de reize naar Groenland, gedaan door Commandeur Maarten Mooy, met het schip Frankendaal. Behelzende zyne uitreize van Amsterdam den 22. April 1786., bezetting in het ys, zedert den 10 Juny, het voorgevallene met de Commandeurs Hans Christian Jaspers, van Altona, Matthieu Weatherhead van London, William Allen, von Hull, en Volkert Klaasen, of Jung Volkert Knudten, van Gottenburg.

In case.

Reklam i sociala medier och dess påverkan på konsumenterna : En kvalitativ studie om generation y’s attityder till reklam på sociala medier

Denna studie syftar till att skapa en förståelse för generation y’s attityder till reklam på dagens fyra största sociala medier. Generation y innefattar individer födda mellan år 1980-1999. Dessa individer har vuxit upp i en tid med framfart av teknologiska produkter där majoriteten använder sociala medier mer eller mindre dagligen. Denna grupp individer anses därmed bli det mest betydelsefulla kundsegmentet för digital marknadsföring i framtiden. För att kunna besvara syftet har en kvalitativ metod tillämpats. Författarna utgick först från teorier som låg till grund för den empiriska data som samlades in, för att slutligen analysera resultatet tillbaka till teorierna. Detta utgjordes av fokusgruppsintervjuer för att på så vis undersöka och erhålla förståelse om individers attityder gällande reklam på sociala medier. Vad som framkom av denna studie är att generation y i studien tolkas ha en positiv attityd till reklam från företag de själva valt att följa på sociala medier. Individerna tolkas även ta till sig mer av reklam som är riktad personligt mot dem. I vilken utsträckning individerna tar till sig av reklamen kopplas även ihop med vilket socialt medium den riktas från. Vidare kan resultatet av denna studie ge dagens och framtida marknadsförare en insikt i hur det betydande marknadssegmentet generation y uppfattar reklam i sociala medier, vilket kan möjliggöra marknadsföringsinnovationer hos företagen som vill stärka varumärket och påverka konsumenternas köpintention positivt

Gastrointestinal Involvement In Lipoid Proteinosis: A Ten-year Follow-up Of A Brazilian Female Patient.

Lipoid proteinosis is a rare autosomal recessive disease characterized by the deposition of hyaline material in the skin and internal organs. The main clinical features are hoarseness and typical skin lesions. In this report we describe the endoscopic and radiologic findings in a Brazilian female patient presenting extensive gastrointestinal involvement and the evolution of the detected lesions in ten years of follow-up. Initial upper endoscopy and colonoscopy showed a similar pattern of multiple yellowish nodules throughout the esophagus, stomach, duodenum, and colons. Histological analysis confirmed the diagnosis of lipoid proteinosis. In addition, small bowel follow through demonstrated numerous well defined, round, small filling defects throughout the jejunum. Ten years later, the esophageal lesions remained the same, but none of the previous alterations were detected in the stomach, duodenum, and colons. In conclusion, lipoid proteinosis may affect all gastrointestinal organs with the same pattern of macroscopic and microscopic lesions. Some lesions may regress with increasing age.