Planktonic foraminifera of sediment trap samples

Biweekly sediment trap samples and concurrent hydrographic measurements collected between March 2005 and October 2008 from the Cariaco Basin, Venezuela, are used to assess the relationship between [CO3]2- and the area densities (ho A) of two species of planktonic foraminifera (Globigerinoides ruber (pink) and Globigerinoides sacculifer). Calcification temperatures were calculated for each sample using species-appropriate oxygen isotope (d18O) temperature equations that were then compared to monthly temperature profiles taken at the study site in order to determine calcification depth. Ambient [CO3]2- was determined for these calcification depths using alkalinity, pH, temperature, salinity, and nutrient concentration measurements taken during monthly hydrographic cruises. The rho A, which is representative of calcification efficiency, is determined by dividing individual foraminiferal shell weights (±0.43 µg) by their associated silhouette areas and taking the sample average. The results of this study show a strong correlation between rho A and ambient [CO3]2- for both G. ruber and G. sacculifer (R**2 = 0.89 and 0.86, respectively), confirming that [CO3]2- has a pronounced effect on the calcification of these species. Though the rho A for both species reveal a highly significant (p < 0.001) relationship with ambient [CO3]2-, linear regression reveals that the extent to which [CO3]2- influences foraminiferal calcification is species specific. Hierarchical regression analyses indicate that other environmental parameters (temperature and [PO4]3-) do not confound the use of G. ruber and G. sacculifer rho A as a predictor for [CO3]2-. This study suggests that G. ruber and G. sacculifer rho A can be used as reliable proxies for past surface ocean [CO3]2?-

Stable oxygen isotope and Mg/Ca ratios on Globorotalia inflata

We present a species-specific Mg/Ca-calcification temperature calibration for Globorotalia inflata from a suite of 38 core top samples from the South Atlantic (from 8° to 49°S). G. inflata is a deep-dwelling planktonic foraminifer commonly occurring in subtropical to subpolar conditions, which qualifies it for reconstructions of the permanent thermocline. Apparent calcification depths and calcification temperatures were determined by comparing measured d18O with equilibrium d18O of calcite based on water column properties. Based on our core top samples, G. inflata apparent calcification depth is constant throughout the South Atlantic mid-latitudes with a depth of 350-400 m within the permanent thermocline. The resulting Mg/Ca-calcification temperature calibration is Mg/Ca = 0.72 +/-0.045/0.042 exp (0.076 +0.006 calcification 2 temperature) (r2 = 0.81) and covers the temperature range 3.1-16.5°C. We applied our Mg/Ca calibration to gravity core PS2495-3 from the Mid-Atlantic Ridge at ca. 41°S to test its validity by reconstructing a low-resolution record covering the last two glacial-interglacial cycles. Our paleotemperature record reveals large changes in temperature for Terminations I and II, when permanent thermocline temperature increased by as much as 8°C. The G. inflata paleotemperature record suggests that oceanic fronts repeatedly migrated over the location of site PS2495-3 during the last 160 kyr. This study shows the potential of G. inflata Mg/Ca to reconstruct paleotemperatures in the permanent thermocline.

Investigation of planktic foraminifera from the Norwegian-Greenland Sea

The vertical density gradients in the Nordic Seas are crucial for the preconditioning of the surface water to thermohaline sinking in winter. These gradients can be reconstructed from paired oxygen isotope data in tests of different species of planktonic foraminifera, the isotopic signatures of which represent different calcification depths in the water column. Comparison of d18O values from foraminiferal tests in plankton hauls, sediment traps, and nearby core top samples with the calculated d18Ocalcite profile of the water column revealed species-specific d18O vital effects and the role of bioturbational admixture of subfossil specimens into the surface sediment. On the basis of core top samples obtained along a west-east transect across various hydrographic regions of the Nordic Seas, d18O values of Turborotalita quinqueloba document apparent calcification depths within the pycnocline at 25-75 m water depth. The isotopic signatures of Neogloboquadrina pachyderma (s) reflect water masses near and well below the pycnocline between 70 and 250 m off Norway, where the Atlantic inflow leads to thermal stratification. Here, temperatures in the calcification depth of N. pachyderma (s) differ from sea surface temperature by approximately -2.5°C. In contrast, N. pachyderma (s) calcifies very close to the sea surface (20-50 m) in the Arctic domain of the western Nordic Seas. However, further west N. pachyderma (s) prefers somewhat deeper, more saline water at 70-130 m well below the halocline that confines the low saline East Greenland Current. This implies that the d18O values of N. pachyderma (s) do not fully reflect the freshwater proportion in surface water and that any reconstruction of past meltwater plumes based on d18O is too conservative, because it overestimates sea surface salinity. Minimum d18O differences (<0.2per mil) between N. pachyderma (s) and T. quinqueloba may serve as proxy for sea regions with dominant haline and absent thermal stratification, whereas thermal stratification leads to d18O differences of >0.4 to >1.5per mil.

Multinet and water column properties of station SO225-21

Insight into past changes of upper ocean stratification, circulation, and nutrient signatures rely on our knowledge of the apparent calcification depth (ACD) and ecology of planktonic foraminifera, which serve as archives for paleoceanographic relevant geochemical signals. The ACD of different species varies strongly between ocean basins, but also regionally. We constrained foraminiferal ACDs in the western Pacific warm pool (Manihiki Plateau) by comparing stable oxygen and carbon isotopes (d18Ocalcite, d13Ccalcite) as well as Mg/Ca ratios from living planktonic foraminifera to in-situ physical and chemical water mass properties (temperature, salinity, d18Oseawater, d13CDIC). Our analyses point to Globigerinoides ruber as the shallowest dweller, followed by Globigerinoides sacculifer, Neogloboquadrina dutertrei, Pulleniatina obliquiloculata and Globotaloides hexagonus inhabiting increasingly greater depths. These findings are consistent with other ocean basins; however, absolute ACDs differ from other studies. The uppermost mixed-layer species G. ruber and G. sacculifer denote mean calcification depths of ~95 m and ~120 m, respectively. These Western Pacific ACDs are much deeper than in most other studies and most likely relate to the thick surface mixed layer and the deep chlorophyll maximum in this region. Our results indicate that N. dutertrei appears to be influenced by mixing waters from the Pacific equatorial divergence, while P. obliquiloculata with an ACD of ~160 m is more suitable for thermocline reconstructions. ACDs of G. hexagonus reveal a deep calcification depth of ~450 m in oxygen-depleted, but nutrient-rich water masses, consistent to other studies. As the d13C of G. hexagonus is in near-equilibrium with ambient seawater, we suggest this species is suitable for tracing nutrient conditions in equatorial water masses originating in extra-topical regions.

Productores agropecuarios, pampa ondulada y problemáticas edáficas. Un estudio de caso en el noreste bonaerense

El carácter de la heterogeneidad social, económica y productiva de la agriculturización se evidencia en los diversos tipos de productores existentes en la pampa húmeda. La agriculturización se asocia a modificaciones en la calidad de las tierras, en la estructura socio-productiva, en las estrategias productivas aplicadas y en las formas de uso del suelo. Suele plantearse sin embargo que las problemáticas edáficas asociadas a dicha agriculturización, son consecuencia de la aplicación de paquetes tecnológicos relativamente homogéneos, independientemente de los diferentes tipos de productores que los llevan a cabo. Nuestra hipótesis consiste en cambio, en que el deterioro en Argiudoles típicos pampeanos es el resultado, dadas distintas posiciones del relieve, de complejas combinaciones de diversas estrategias productivas adoptadas por diferentes tipos de productores. En el partido de Luján tipificamos los productores tomando en consideración sus niveles de capitalización (capitalizados y no capitalizados) y su organización del trabajo (familiar y no familiar). Definimos cinco estrategias productivas (4 agrícolas -con uno o dos cultivos por año, con siembra directa o labranza convencional- y 1 ganadera) y dos ambientes (loma y bajo). A partir del catastro municipal aplicamos una encuesta a una muestra estratificada estadísticamente representativa, por ubicación y tamaño de lotes. Sobre la misma realizamos el muestreo que nos permitió analizar los siguientes parámetros: profundidad del horizonte, densidad aparente, materia orgánica, acidez, nitrógeno, fósforo, potasio. Calculamos el contenido de materia orgánica y de nitrógeno por hectárea, y el deterioro relativo. Realizamos test de hipótesis de comparación de medias, prueba F y prueba t; y, calculamos finalmente los deterioros relativos. Utilizamos como indicador el contenido de materia orgánica por hectárea, dada su mayor sensibilidad a los cambios en las condiciones del suelo. Dos de nuestros principales hallazgos nos indican, en primer lugar, que al cultivar en lomas y con siembra directa, todos los tipos de productores presentaron los valores más bajos de deterioro relativo, excepto los familiares no capitalizados que obtienen los menores deterioros relativos con labranza convencional, aún en los bajos. Estos productores utilizan la ganadería en rotación con los cultivos como táctica de cuidado del suelo. Los empresarios capitalizados presentan los mayores valores de deterioro relativo en los bajos. Los familiares presentan menores pérdidas, cuando son capitalizados logran las mejores situaciones relativas al aplicar siembra directa en los bajos. En segundo lugar, ninguna dimensión -tipo de productor, estrategias productivas, ambientes- analizada aisladamente determina el deterioro relativo de los suelos. Pero, al mismo tiempo, ninguno puede ser descartado sino que debería ser incluido en una combinación que integre las condiciones de ambiente y las estrategias productivas de los diferentes tipos de productores que manejan los suelos.

Productores agropecuarios, pampa ondulada y problemáticas edáficas. Un estudio de caso en el noreste bonaerense

El carácter de la heterogeneidad social, económica y productiva de la agriculturización se evidencia en los diversos tipos de productores existentes en la pampa húmeda. La agriculturización se asocia a modificaciones en la calidad de las tierras, en la estructura socio-productiva, en las estrategias productivas aplicadas y en las formas de uso del suelo. Suele plantearse sin embargo que las problemáticas edáficas asociadas a dicha agriculturización, son consecuencia de la aplicación de paquetes tecnológicos relativamente homogéneos, independientemente de los diferentes tipos de productores que los llevan a cabo. Nuestra hipótesis consiste en cambio, en que el deterioro en Argiudoles típicos pampeanos es el resultado, dadas distintas posiciones del relieve, de complejas combinaciones de diversas estrategias productivas adoptadas por diferentes tipos de productores. En el partido de Luján tipificamos los productores tomando en consideración sus niveles de capitalización (capitalizados y no capitalizados) y su organización del trabajo (familiar y no familiar). Definimos cinco estrategias productivas (4 agrícolas -con uno o dos cultivos por año, con siembra directa o labranza convencional- y 1 ganadera) y dos ambientes (loma y bajo). A partir del catastro municipal aplicamos una encuesta a una muestra estratificada estadísticamente representativa, por ubicación y tamaño de lotes. Sobre la misma realizamos el muestreo que nos permitió analizar los siguientes parámetros: profundidad del horizonte, densidad aparente, materia orgánica, acidez, nitrógeno, fósforo, potasio. Calculamos el contenido de materia orgánica y de nitrógeno por hectárea, y el deterioro relativo. Realizamos test de hipótesis de comparación de medias, prueba F y prueba t; y, calculamos finalmente los deterioros relativos. Utilizamos como indicador el contenido de materia orgánica por hectárea, dada su mayor sensibilidad a los cambios en las condiciones del suelo. Dos de nuestros principales hallazgos nos indican, en primer lugar, que al cultivar en lomas y con siembra directa, todos los tipos de productores presentaron los valores más bajos de deterioro relativo, excepto los familiares no capitalizados que obtienen los menores deterioros relativos con labranza convencional, aún en los bajos. Estos productores utilizan la ganadería en rotación con los cultivos como táctica de cuidado del suelo. Los empresarios capitalizados presentan los mayores valores de deterioro relativo en los bajos. Los familiares presentan menores pérdidas, cuando son capitalizados logran las mejores situaciones relativas al aplicar siembra directa en los bajos. En segundo lugar, ninguna dimensión -tipo de productor, estrategias productivas, ambientes- analizada aisladamente determina el deterioro relativo de los suelos. Pero, al mismo tiempo, ninguno puede ser descartado sino que debería ser incluido en una combinación que integre las condiciones de ambiente y las estrategias productivas de los diferentes tipos de productores que manejan los suelos.

Productores agropecuarios, pampa ondulada y problemáticas edáficas. Un estudio de caso en el noreste bonaerense

El carácter de la heterogeneidad social, económica y productiva de la agriculturización se evidencia en los diversos tipos de productores existentes en la pampa húmeda. La agriculturización se asocia a modificaciones en la calidad de las tierras, en la estructura socio-productiva, en las estrategias productivas aplicadas y en las formas de uso del suelo. Suele plantearse sin embargo que las problemáticas edáficas asociadas a dicha agriculturización, son consecuencia de la aplicación de paquetes tecnológicos relativamente homogéneos, independientemente de los diferentes tipos de productores que los llevan a cabo. Nuestra hipótesis consiste en cambio, en que el deterioro en Argiudoles típicos pampeanos es el resultado, dadas distintas posiciones del relieve, de complejas combinaciones de diversas estrategias productivas adoptadas por diferentes tipos de productores. En el partido de Luján tipificamos los productores tomando en consideración sus niveles de capitalización (capitalizados y no capitalizados) y su organización del trabajo (familiar y no familiar). Definimos cinco estrategias productivas (4 agrícolas -con uno o dos cultivos por año, con siembra directa o labranza convencional- y 1 ganadera) y dos ambientes (loma y bajo). A partir del catastro municipal aplicamos una encuesta a una muestra estratificada estadísticamente representativa, por ubicación y tamaño de lotes. Sobre la misma realizamos el muestreo que nos permitió analizar los siguientes parámetros: profundidad del horizonte, densidad aparente, materia orgánica, acidez, nitrógeno, fósforo, potasio. Calculamos el contenido de materia orgánica y de nitrógeno por hectárea, y el deterioro relativo. Realizamos test de hipótesis de comparación de medias, prueba F y prueba t; y, calculamos finalmente los deterioros relativos. Utilizamos como indicador el contenido de materia orgánica por hectárea, dada su mayor sensibilidad a los cambios en las condiciones del suelo. Dos de nuestros principales hallazgos nos indican, en primer lugar, que al cultivar en lomas y con siembra directa, todos los tipos de productores presentaron los valores más bajos de deterioro relativo, excepto los familiares no capitalizados que obtienen los menores deterioros relativos con labranza convencional, aún en los bajos. Estos productores utilizan la ganadería en rotación con los cultivos como táctica de cuidado del suelo. Los empresarios capitalizados presentan los mayores valores de deterioro relativo en los bajos. Los familiares presentan menores pérdidas, cuando son capitalizados logran las mejores situaciones relativas al aplicar siembra directa en los bajos. En segundo lugar, ninguna dimensión -tipo de productor, estrategias productivas, ambientes- analizada aisladamente determina el deterioro relativo de los suelos. Pero, al mismo tiempo, ninguno puede ser descartado sino que debería ser incluido en una combinación que integre las condiciones de ambiente y las estrategias productivas de los diferentes tipos de productores que manejan los suelos.

Seawater samples along several inshore-offshore transects off NW Africa and SE Africa

For the optimal use in palaeoceanographic studies of the stable oxygen isotopic signal and elemental composition of the calcareous photosynthetic dinoflagellate Thoracosphaera heimii, it is essential to gain detailed information about its calcification depth and spatial distribution. We therefore studied the vertical and horizontal distribution patterns of T. heimii in the upper water column (0-200 m) along three transects: an inshore-offshore gradient off Cape Blanc (CB), a south-north transect from CB to the Portuguese coast and a north-south transect off Tanzania. We compared concentrations of living cysts (cells with cell content) with chlorophyll-a, salinity and temperature measurements at the sampling depth. In order to explore the seasonal variability in cyst production, three transect off CB were sampled at three different times of the year. Living T. heimii cysts were found in the upper 160 m of the water column with highest concentrations in the photic zone indicating that the calcification of T. heimii occurs in the upper part of the water column. Maximal abundances of living cysts were found relatively often in or just above the deep chlorophyll maximum (DCM), the depth of which varies regionally from about 20-40 m off CB to about 80 m off Tanzania and along the transect from CB to the Portuguese Coast. However, there was no significant correlation at the 95% confidence level between the cyst concentrations and temperature, salinity and chlorophyll-a concentrations at the sampling depths observed. In both the Atlantic and Indian Oceans, the highest abundances of T. heimii were observed in regions where the upper water masses contained relatively low nutrient concentrations that are influenced only sporadically, or not at all, by enhanced photic zone mixing related to the presence of upwelling cells or river outflow plumes at or close to the sampling sites. The seasonal production of cysts by T. heimii appears to be negatively related to the presence of upwelling filaments across the sampling sites. Our study suggests that turbulence of the upper water masses is a major environmental factor influencing T. heimii production.

Stable isotope record of planktonic foraminifera of the Atlantic Ocean

The thermal structure of the upper ocean (0-1000 m) is set by surface heat fluxes, shallow wind-driven circulation, and the deeper thermohaline circulation. Its long-term variability can be reconstructed using deep-dwelling planktonic foraminifera that record subsurface conditions. Here we used six species (Neogloboquadrina dutertrei, Globorotalia tumida, Globorotalia inflata, Globorotalia truncatulinoides, Globorotalia hirsuta, and Globorotalia crassaformis) from 66 core tops along a meridional transect spanning the mid-Atlantic (42°N to 25°S) to develop a method for reconstructing past thermocline conditions. We estimated the calcification depths from d18O measurements and the Mg/Ca-temperature relationships for each species. This systematic strategy over this large latitudinal section reveals distinct populations with different Mg/Ca-temperature relationships for G. inflata, G. truncatulinoides, and G. hirsuta in different areas. The calcification depths do not differ among the different populations, except for G. hirsuta, where the northern population calcifies much shallower than the southern population. N. dutertrei and G. tumida show a remarkably constant calcification depth independent of oceanographic conditions. The deepest dweller, G. crassaformis, apparently calcifies in the oxygen-depleted zone, where it may find refuge from predators and abundant aggregated matter to feed on. We found a good match between its calcification depth and the 3.2 ml/l oxygen level. The results of this multispecies, multiproxy study can now be applied down-core to facilitate the reconstruction of open-ocean thermocline changes in the past.

Stable carbon and oxygen isotope composition of benthic foraminifera

Seventeen surface sediment samples from the North Atlantic Ocean off NE-Greenland between 76° and 81°N, and nine samples from the South Atlantic Ocean close to Bouvet Island between 48° and 55°S were taken with the aid of a Multiple Corer and investigated for their live (Rose Bengal stained) benthic foraminiferal content within the upper 15 cm of sediment. Preferentially endobenthic Melonis barleeanum, Melonis zaandami, and Bulimina aculeata as well as preferentially epibenthic Lobatula lobatula were counted from 1-cm-thick sediment slices each and analyzed for stable carbon and oxygen isotopic compositions of their calcareous tests. Live and dead specimens were counted and measured separately. The carbon isotopic composition of the foraminifera was compared to that of the dissolved inorganic carbon (DIC) of simultaneously sampled bottom water. During a period of one month, one station off NE-Greenland was replicately sampled once every week and samples were processed as above. Live specimens of Lobatula lobatula are confined to the uppermost two centimeters of sediment. Live specimens of Melonis spp. are found down to 8 cm within the sediment but with a distinct sub-surface maximum between 2 and 5 cm. The down-core distribution of live Bulimina aculeata shows a distinct surface maximum in the top centimeter and constant but low numbers down to 11-cm subbottom depth. The average stable carbon isotopic composition (d13C versus per mil PDB) of live Lobatula lobatula off NE-Greenland is by 0.4±0.1 per mil higher than the d13CDIC of the ambient bottom water at the time of sampling. There is evidence that this species calcify before the ice-free season, when bottom water d13CDIC is supposed to be higher. This would reconfirm the one-to-one relationship between d13C of ambient water DIC and cibicids, widely used by paleoceanographers. Live Melonis barleeanum show a negative offset from bottom water DIC of -1.7±0.6 per mil in the uppermost sediment and of -2.2±0.5 per mil in 3-4-cm subbottom depth. All d13C values of live Melonis spp. decrease within the upper four centimeters, regardless of the time of sampling and site investigated. The offset of live Bulimina aculeata from bottom water d13CDIC values of 8 stations rather constantly amounts to -0.6±0.1 per mil, no matter what subbottom depth the specimens are from. At one station however, where is strong indication of elevated organic carbon flux, the negative offset averaged over all sub-bottom depths increases to -1.5±0.2 per mil. Buliminids actively move within the sediment and by this either record an average isotope signal of the pore water or the signal of one specific calcification depth. The recorded signal, however, depends on the organic carbon flux and reflects general but site-specific pore water d13CDIC values. If compared with epibenthic d13C values from the same site, not influenced by pore water and related phytodetritus layer effects, Buliminad13C values bear some potential as a paleoproductivity proxy. Specimens of Melonis spp. seem to prefer a more static way of life and calcify at different but individually fix depths within the sediment. Although live specimens thus record a stratified pore water d13C signal, there is no means yet to correct for bioturbational and early diagenetic effects in fossil faunas.

(Table 1) Core top Globorotalia truncatulinoides d18O ratios of Atlantic Ocean samples

We investigate whether the oxygen isotope ratio in the test of Globorotalia truncatulinoides can serve as a proxy for intermediate depth (200-500 m) density. Since intermediate depth horizontal density gradients are associated with the vertical shear of upper ocean flows, this proxy could provide a tool for reconstructing past ocean circulation. The spatial pattern of core top Gr. truncatulinoides d18O in the Atlantic Ocean mimics the upper ocean density gradients associated with the major ocean currents. To better constrain the controls on the calcification depth(s) of Gr. truncatulinoides, we attempt to simulate the surface sediment data set using water column temperature and salinity conditions above the core sites. We predicted foraminiferal d18O for each core site assuming (1) the calcification occurs at a single depth and (2) the initial calcification is at the surface and the subsequent calcification is at 800 m water depth. The predicted d18O best resembles measured d18O of Gr. truncatulinoides when using (1) a single depth calcification at 350 m or (2) a two-depth approximation with 30% surface and 70% 800-m calcification. This result gives us confidence in the ability of d18O in Gr. truncatulinoides to proxy lateral density gradients at the intermediate depths associated with upper ocean flow.

Stable oxygen isotope composition of planktonic foraminifera in the Canary Islands region

Seasonal depth stratified plankton tows, sediment traps and core tops taken from the same stations along a transect at 29°N off NW Africa are used to describe the seasonal succession, the depth habitats and the oxygen isotope ratios (delta18O(shell)) of five planktic foraminiferal species. Both the delta18O(shell) and shell concentration profiles show variations in seasonal depth habitats of individual species. None of the species maintain a specific habitat depth exclusively within the surface mixed layer (SML), within the thermocline, or beneath the thermocline. Globigerinoides ruber (white) and (pink) occur with moderate abundance throughout the year along the transect, with highest abundances in the winter and summer/fall season, respectively. The average delta18O(shell) of G. ruber (w) from surface sediments is similar to the delta18O(shell) values measured from the sediment-trap samples during winter. However, the delta18O(shell) of G. ruber (w) underestimates sea surface temperature (SST) by 2 °C in winter and by 4 °C during summer/fall indicating an extension of the calcification/depth habitat into colder thermocline waters. Globigerinoides ruber (p) continues to calcify below the SML as well, particularly in summer/fall when the chlorophyll maximum is found within the thermocline. Its vertical distribution results in delta18O(shell) values that underestimate SST by 2 °C. Shell fluxes of Globigerina bulloides are highest in summer/fall, where it lives and calcifies in association with the deep chlorophyll maximum found within the thermocline. Pulleniatina obliquiloculata and Globorotalia truncatulinoides, dwelling and calcifying a part of their lives in the winter SML, record winter thermocline (~180 m) and deep surface water (~350 m) temperatures, respectively. Our observations define the seasonal and vertical distribution of multiple species of foraminifera and the acquisition of their delta18O(shell).

Planktonic foraminiferal d18O values and apparent calcification depths of core-top samples along an east-west transect across the tropical Atlantic/Caribbean

D18O values of nine tropical-subtropical planktonic foraminiferal species with different preferential habitat depths collected from 62 core-top samples along an east-west transect across the tropical Atlantic/Caribbean were used to test the applicability of interspecific d18O gradients for reconstructions of tropical upper ocean stratification. In general, the d18O difference (Delta d18O) between intermediate- and shallow-dwelling species decreases, and Delta d18O between deep and intermediate dwellers increases with increasing thermocline depth towards the west. The statistical significance of regional differences in Delta d18O highlights Delta d18O between the intermediate dwellers (in particular Globorotalia scitula and Globorotalia tumida) and the shallow dweller Globigerinoides ruber pink, as well as Delta d18O between the deep dwellers Globorotalia crassaformis or Globorotalia truncatulinoides dextral and intermediate dwellers as most sensitive to changes in tropical Atlantic thermocline depth. Based on the observed regional variations in interspecific Delta d18O, we propose a multispecies stratification index "STRAtrop" = (d18Ointermediate - d18Oshallow) / (d18Odeep - d18Oshallow) for the tropical ocean. Statistically significant differences in STRAtrop values between the E-Atlantic and the Caribbean suggest that this index may be a useful tool to monitor variations in tropical upper ocean stratification in the geological record.