Culture medium of diluted skimmed milk for the production of nisin in batch cultivations

Nisin is a promising alternative to chemical preservatives for use as a natural biopreservative in foods. This bacteriocin has also potential biomedical applications. Lactic acid bacteria are commonly cultivated in expensive standard complex media. We have evaluated the cell growth and nisin production of Lactococcus lactis in a low-cost natural medium consisting of diluted skimmed milk in a 2-L bioreactor. The assays were performed at 30 degrees C for 56 h, at varying agitation speeds and airflow rates: (1) 200 rpm (no airflow, and airflow at 0.5, 1.0 and 2.0 L/min); (2) 100 rpm (no airflow, and airflow at 0.5 L/min). Nisin activity was evaluated using agar diffusion assays. The highest nisin concentration, 49.88 mg/L (3.3 log AU/mL or 1,995.29 AU/mL), was obtained at 16 h of culture, 200 rpm and no airflow (k(L)a = 5.29 x 10(-3)). These results show that a cultivation medium composed of diluted skimmed milk supports cell growth to facilitate nisin biosynthesis.

Oeuvres complètes de Voltaire. Année 1711-1735,Numéro 1-539 / nouvelle édition... précédée de la Vie de Voltaire, par Condorcet et d'autres études biographiques

Comprend : Vie de Voltaire

Iowa DOT Evaluation of the PASCO Road Survey System, HR-539, 1987

The report compares and contrasts the automated PASCO method of pavement evaluation to the manual procedures used by the Iowa Department of Transportation (DOT) to evaluate pavement condition. Iowa DOT's use of IJK and BPR roadmeters and manual crack and patch surveys are compared to PASCO's use of 35-mm photography, artificial lighting and hairline projection, tracking wheels and lasers to measure ride, cracking and patching, rut depths, and roughness. The Iowa DOT method provides a Present Serviceability Index (PSI) value and PASCO provides a Maintenance Control Index (MCI). Seven sections of Interstate Highway, county roads and city streets, and one shoulder section were tested with different speeds of data collection, surface types and textures, and stop and start conditions. High correlation of results between the two methods in the measurement of roughness (0.93 for the tracking wheel and 0.84 for the laser method) were recorded. Rut depth correlations of 0.61 and cracking of 0.32 are attributed to PASCO's more comprehensive measurement techniques. A cost analysis of the data provided by both systems indicates that PASCO is capable of providing a comparable result with improved accuracy at a cost of $125-$150 or less per two-lane mile depending on survey mileage. Improved data collection speed, accuracy, and reliability, and a visible record of pavement condition for comparable costs are available. The PASCO system's ability to provide the data required in the Highway Pavement Distress Identification Manual, the Pavement Condition Rating Guide, and the Strategic Highway Research Program Long Term Pavement Performance (LTPP) Studies, is also outlined in the report.

El consumo de probiótico Lactobacillus plantarum CECT 7315/7316 mejora el estado de salud general en personas de edad avanzada

Fundamentos: Con la edad avanzada se producen cambios en la microflora intestinal que pueden afectar al estado de salud general. En este trabajo analizamos el efecto de Lactobacillus plantarum CECT 7315/7316 sobre la regulación del tránsito intestinal y el estado nutricional. Métodos: Hemos realizado un estudio clínico dobleciego, controlado por placebo y aleatorizado. Hemos evaluado la evolución de la frecuencia de defecación semanal y los niveles en sangre de proteínas totales, albúmina,colesterol y proteína C-reactiva. Resultados: Lactobacillus plantarum CECT 7315/7316 ayuda a regular el tránsito intestinal y mejora el estado nutricional en personas mayores. Conclusiones: El consumo de productos funcionales que contengan L. plantarum CECT 7315/7316 mejora la calidad de vida de personas de la tercera edad.

Na 50 Nachlass Arthur Schopenhauer - 'Schopenhauer-Archiv', 539 - Konzeptschreiben für den Kapellmeister der Frankfurter Oper Carl Wilhelm Ferdinand Guhr mit enthaltener Beschwerde über die schlechten Zustände in der Frankfurter Oper

veröffentlicht in: Arthur Hübscher (Hrsg), Arthur Schopenhauer Gesammelte Briefe, Bonn 1978, Nr.203

Na 1 Nachlass Max Horkheimer, 539 - Korrespondenzen mit Leo Löwenthal und Friedrich Pollock (p. VI 15, 1 - 206)

113 Briefe zwischen Max Horkheimer und Leo Löwenthal; 1 Brief von Leo Löwenthal an Moritz Horkheimer, 25.08.1942; 1 Brief von Leo Löwenthal an Alfredo Mendizabel, 19.08.1942; 1 Brief von Eileen A. Fry an Max Horkheimer, 19.08.1942; 4 Briefe zwischen Leo Löwenthal und Frederick Pollock, 1942; 1 Brief von Max Horkheimer an Frederick Pollock, 11.12.1942;

Ms.Ff.F.Stoltze 3. 539 - Brief von Friedrich Stoltze an Unbekannt

Korrektur zu einem Gelegenheitsgedicht

Daniel Bensaïd (2009). Marx Intempestivo. Grandezas y miserias de una aventura crítica. Buenos Aires: Ediciones Herramienta. 539 páginas

Fil: Franchella, Agustín. Universidad Nacional de La Plata. Facultad de Humanidades y Ciencias de la Educación; Argentina.

Daniel Bensaïd (2009). Marx Intempestivo. Grandezas y miserias de una aventura crítica. Buenos Aires: Ediciones Herramienta. 539 páginas

Fil: Franchella, Agustín. Universidad Nacional de La Plata. Facultad de Humanidades y Ciencias de la Educación; Argentina.

Daniel Bensaïd (2009). Marx Intempestivo. Grandezas y miserias de una aventura crítica. Buenos Aires: Ediciones Herramienta. 539 páginas

Fil: Franchella, Agustín. Universidad Nacional de La Plata. Facultad de Humanidades y Ciencias de la Educación; Argentina.