Estudo de efeitos de um extrato de Arctium lappa (Bardana) na marca��ão dos constituintes sangüíneos com Tecnécio-99m, na biodisponibilidade do radiofármaco pertecnatato de sódio e na morfologia de hemácias de ratos Wistar

Radionuclides have been used in Nuclear Medicine for diagnostic and treatment. In basic research, cellular and molecular structures are labeled with technetium-99m (99mTc) and used as radiobiocomplexes. Some natural or synthetic drugs are capable to alter the labeling of blood constituents with 99mTc, as well as in the biodistribution of radiobiocomplexes. Arctium lappa (Bardana) has been used to treat inflammatory processes. The aim of this work was to evaluate the effects of an extract of Bardana on the labeling of blood constituents with 99mTc, on the morphology of red blood cells, on the perimeter/area ratio of red blood cells and on the biodistribution of radiophamaceutical sodium pertechnetate in Wistar rats. Extract of Bardana was capable to alter the labeling of cellular compartment with 99mTc. Plasma and cellular proteins did not present alteration on the percentage of radioactivity (%ATI). Extract of Bardana was also capable to alter the morphology and the perimeter/area ratio of red blood cells. On the biodistribution of sodium pertechnetate in animals treated with the extract of Bardana, it was observed a small and significant uptake in liver, tooth and tongue, and a high and a significant uptake in stomach, lung and testis (p<0.05). In conclusion, these findings could be justified due to the effects of some chemical compounds in the Bardana extract. This study was a multidisciplinary experimental research. It was developed with the contribution of the different Departments and Services of the Hospital Universitário Pedro Ernesto of the Universidade Estadual do Rio de Janeiro

Anti-inflammatory intestinal activity of Arctium lappa L. (Asteraceae) in TNBS colitis model

Ethnopharmacological relevance: In Brazilian traditional medicine, Arctium lappa (Asteraceae), has been reported to relieve gastrointestinal symptoms. Aim of the study: In the present study, we investigated the effects of the lactone sesquiterpene onopordopicrin enriched fraction (ONP fraction) from Arctium lappa in an experimental colitis model induced by 2,4,6 trinitrobenzene sulfonic acid and performed experiments to elucidate the underlying action mechanisms involved in that effect. Materials and methods: ONP fraction (25 and 50 mg/kg/day) was orally administered 48, 24 and 1 h prior to the induction of colitis and 24 h after. The inflammatory response was assessed by gross appearance, myeloperoxidase (MPO) activity, tumor necrosis factor alpha (TNF-α) levels and a histological study of the lesions. We determined cyclooxygenase (COX)-1 and -2 protein expressions by western blotting and immunohistochemistry assays. Results: TNBS group was characterized by increased colonic wall thickness, edema, diffuse inflammatory cell infiltration, increased MPO activity and TNF-α levels. On the contrary, ONP fraction (25 and 50 mg/kg) treatment significantly reduced the macroscopic inflammation scores (p<0.05 and p<0.01, respectively) and morphological alterations associated with an increase in the mucus secretion. Similarly, the degree of neutrophil infiltration and the cytokine levels were significantly ameliorated. Moreover, COX-2 expression was up regulated in TNBS-treated rats. In contrast, ONP fraction (50 mg/kg) administration reduced COX-2 overexpression. Conclusions: We have shown that the ONP fraction obtained from Arctium lappa exert marked protective effects in acute experimental colitis, confirming and justifying, at least in part, the popular use of this plant to treat gastrointestinal diseases. © 2013 Elsevier B.V. All rights reserved.

Hepatoprotective Effect of Arctium lappa Root Extract on Cadmium Toxicity in Adult Wistar Rats

Funda��ão de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Control of microorganisms of oral health interest with Arctium lappa L. (burdock) extract non-cytotoxic to cell culture of macrophages (RAW 264.7)

Objectives: To evaluate the antimicrobial activity of Arctium lappa L. extract on Staphylococcus aureus, S. epidermidis, Streptococcus mutans, Candida albicans, C. tropicalis and C. glabrata. In addition, the cytotoxicity of this extract was analyzed on macrophages (RAW 264.7).Design: By broth microdilution method, different concentrations of the extract (250-0.4 mg/mL) were used in order to determine the minimum microbicidal concentration (MMC) in planktonic cultures and the most effective concentration was used on biofilms on discs made of acrylic resin. The cytotoxicity A. lappa L. extract MMC was evaluated on RAW 264.7 by MTT assay and the quantification of IL-1 beta and TNF-alpha by ELISA.Results: The most effective concentration was 250 mg/mL and also promoted significant reduction (log(10)) in the biofilms of S. aureus (0.438 +/- 0.269), S. epiderrnidis (0.377 +/- 0.298), S. mutans (0.244 +/- 0.161) and C. albicans (0.746 +/- 0.209). Cell viability was similar to 100%. The production of IL-beta was similar to the control group (p > 0.05) and there was inhibition of TNF-alpha (p < 0.01).Conclusions: A. lappa L. extract was microbicidal for all the evaluated strains in planktonic cultures, microbiostatic for biofilms and not cytotoxic to the macrophages. (C) 2014 Elsevier Ltd. All rights reserved.

道地药材牛蒡子适宜产区划分

牛蒡子为两年生菊科植物牛蒡(Arctium lappa L.)的干燥成熟果实,又名大力子。牛蒡在全国各地均有分布,据《中华人民共和国药典》记载,牛蒡子主要有四大产区:东北产区、华东产区、西南产区和华中产区。过去认为牛蒡子以浙江一带所产的为好,目前牛蒡子被认为是关药,以东北产的为佳。目前,有关牛蒡子的道地药材产区仍然存在争议。因此,科学划分牛蒡子的道地产区,不仅可以解决争议,而且对牛蒡的引种栽培具有重要的实践指导意义。本研究运用多元统计方法,确定影响牛蒡子道地性各环境因子的权重,在地理信息系统ArcGis9.0的支持下,进行牛蒡子产地适宜性划分,结果表明:牛蒡的最适宜分布区沿西南-东北方向呈现条带状分布,主要分布在东北、华北和西南地区。与《中华人民共和国药典》中记载的产区基本相近。但是,笔者的研究结果与药典记载也存在差异,华北(包括北京、天津、河北、山西、内蒙古)和西北(宁夏、新疆、陕西、甘肃)也是牛蒡的集中分布区;原来以浙江为主的华东产区,现在以山东为主;东北地区的吉林和黑龙江也是牛蒡子的主产区;西南地区牛蒡子的产区除了四川和重庆外,还包括云南和贵州;在华南和华中地区的分布较少。造成这种情况的原因可能是人为干扰(如人为拔除、除草剂的大量使用等)和气候变化影响。

中国牛蒡种质资源植被调查与采集

<正>牛蒡Arctium lappa L.为菊科药用植物,其干燥成熟果实为牛蒡子,是一味临床常用中药,具有疏散风热、宣肺透疹,解毒利咽的功效,用于治疗风热感冒、咳嗽痰多、麻疹、风疹、咽喉肿痛等症。牛蒡子应用历史悠久,始载于《名医别录》

全人矩矱Quan ren ju he.La règle de tout l'homme.

Contient : I, livre préliminaire太上感應篇纂註Tai shang gan ying pian zuan zhu.Le Tai shang gan ying pian, avec commentaires ; II, livre préliminaire文昌帝君陰隲文纂註Wen chang di jun yin zhi wen zuan zhu.Le Yin zhi wen, du dieu de la Littérature, avec commentaires ; III, livre préliminaire文昌帝君覺世文纂註Wen chang di jun jue shi wen zuan zhu.Traité pour éveiller le monde, par le dieu de la Littérature ; IV, livre préliminaire關聖帝君覺世經 。釋略并諭Guan sheng di jun jue shi jing. Shi lüe bing yu.Le vrai livre sacré du réveil du monde, par le dieu de la Guerre ; V, livre préliminaire純陽祖師警士文Chun yang zu shi jing shi wen.Avertissement aux lettrés, traité du dieu Fu you ; VI, livre préliminaire魏元君勸世文Wei yuan jun quan shi wen ; VII, livre 1勸孝集說Quan xiao ji shuo.Recueil de textes recommandant la piété filiale ; VIII, livre 2戒淫集說Jie yin ji shuo.Recueil de textes contre l'impureté ; IX, livre 2文昌帝君戒淫文Wen chang di jun jie yin wen.Traité contre l'impureté, par le dieu de la Littérature ; X, livre 2文昌帝君天戒錄Wen chang di jun tian jie lu.Défenses célestes, notées par le dieu de la Littérature ; XI, livre 2三丰張眞人戒淫說San feng zhang zhen ren jie yin shuo.Traité contre l'impureté, par l'homme vrai Zhang, de San feng ; XII Livre 2文昌帝君慾海廻狂寶訓Wen chang di jun yu hai hui kuang bao xun.Instructions pour tirer l'insensé de la mer de la concupiscence, par le dieu de la Littérature ; XIII, livre 3勸戒彚抄Quan jie hui chao.Recueil de conseils et de défenses.Wen di quan zhong.Traité pour conseiller la fidélité, par le dieu de la Littérature ; XIV, livre 3文帝戒士子文Wen di jie shi zi wen.Conseils aux lettrés, par le dieu de la Littérature ; XV, livre 3文帝鱣壇勸世文Wen di zhan tan quan shi wen.Conseils donnés au monde par le dieu de la Littérature, à l'autel Zhan ; XVI, livre 3文帝鱣壇語錄Wen di zhan tan yu lu.Entretiens du dieu de la Littérature à l'autel Zhan ; XVII, livre 3蕉窻十則并序Jiao chuang shi ze bing xu.Les dix commandements de Jiao tchhoang, avec préface ; XVIII, livre 3文帝三教論 。七願 。十戒Wen di san jiao lun. Qi yuan. Shi jie.Dissertation sur les trois religions ; les sept vœux ; les dix commandements : du dieu de la Littérature ; XIX, livre 3呂祖師訓世文Lü zu shi xun shi wen.Instructions au monde par le dieu Fu you ; XX, livre 3勸敬惜字紙文Quan jing xi zi zhi wen.Traité pour conseiller le respect des caractères d'écriture ; XXI, livre 3敬字說Jing zi shuo.Traité sur le respect des caractères d'écriture ; XXII, livre 3惜字說Xi zi shuo.Traité sur le ménagement des caractères d'écriture ; XXIII, livre 3勸敬惜五榖文Quan jing xi Wu gu wen.Traité du respect des cinq sortes de grains ; XXIV, livre 3戒殺生說Jie sha sheng shuo.Traité pour défendre de tuer les êtres ayant vie ; XXV, livre 3戒溺女說Jie ni nü shuo.Traité pour défendre de noyer les petites filles ; XXVI, livre 3戒賭文Jie du wen.Traité contre le jeu ; XXVII, livre 3戒嗜酒Jie shi jiu.Défense d'aimer le vin ; XXVIII, livre 3戒貪財Jie tan cai.Défense de convoiter les richesses ; XXIX, livre 3戒聽讒Jie ting chan.Défense d'écouter la calomnie ; XXX, livre 3戒口過文Jie kou ge wen.Traité pour interdire les péchés de langue ; XXXI, livre 3戒損人利己說Jie sun ren li ji shuo.Traité pour interdire de chercher des avantages personnels au détriment d'autrui ; XXXII, livre3不自棄文Bu zi qi wen.Traité recommandant de ne pas s'abandonner au vice ; XXXIII, livre 3戒慢葬Jie man zang.Traité pour défendre d'user de négligence dans les funérailles ; XXXIV, livre 3戒不修治墳墓Jie bu xiu zhi fen mu.Traité recommandant d'entretenir les tombes ; XXXV, livre 3勸塾師培植子弟Quan shu shi pei zhi zi di.Traité recommandant aux ma��tres de prendre soin de leurs élèves ; XXXVI, livre 3勸尊重師傅Quan zun zhong shi fu.Traité recommandant aux élèves d'honorer leur ma��tre ; XXXVII, livre 3勸幕賓Quan mo bin.Conseils aux secrétaires privés des mandarins ; XXXVIII, livre 3勸公門修行Quan gong men xiu xing.Dans les affaires publiques, il faut régler sa conduite ; XXXIX, livre 3勸擇術慎業Quan ze shu shen ye.Il faut choisir un métier et s'y appliquer ; XL, livre 3勸化惡助善Quan hua e zu shan.Il faut corriger le mal et contribuer au bien ; XLI, livre 3勸排難解紛Quan phai nan jie fen.Il faut écarter les obstacles et dissiper les difficultés ; XLII, livre 3勸謙和雍睦Quan qian he yong mu.Traité pour conseiller l'esprit de conciliation et de concorde ; XLIII, livre 3勸救濟Quan jiu ji.Il faut aider (ceux qui ont besoin d'aide) ; XLIV, livre 3賑饑十二善Zhen ji shi er shan.Il faut nourrir les affamés ; XLV, livre 3勸敬說Quan jing shuo.Traité sur le respect ; XLVI, livre 3勸畏說Quan wei shuo.Traité sur la crainte respectueuse ; XLVII, livre3改過之法Gai ge zhi fa.Moyen de corriger les fautes ; XLVIII, livre3勸惜福Quan xi fu.Il faut ménager sa part de bonheur ; XLIX, livre3耕心說Geng xin shuo. Autre titre : 勸耕治心地Quan geng zhi xin di.Il faut cultiver son cœur ; L, livre3廣立命說Guang li ming shuo.Développement du traité sur la direction de la vie ; LI, livre3為學當從事性道Wei xue lang cong shi xing dao.Pour étudier, il faut s'appliquer à la voie naturelle ; LII, livre3性道集說Xing dao ji shuo.Recueil de textes sur la voie naturelle ; LIII, livre3夢覺圖說Mong jue tu shuo.Traité et figure sur l'illusion universelle ; LIV, livre4功過格彚編Gong ge ge hui bian.Liste des mérites et des péchés ; LV, livre4行功過格說Xing gong ge ge shuo.Traité sur l'usage de l'échelle des mérites et des péchés ; LVI, livre4功過格例Gong ge ge li.Règles annexes à l'échelle des mérites et des péchés ; LVII, livre4功過格分類彚編Gong ge ge fen lei hui bian.Liste méthodique des mérites et des péchés ; LVIII, livre 4不費錢功德例Bu fei qian gong de li.Manière d'obtenir des mérites sans dépenser d'argent ; LIX, livre final勸世詩歌Quan shi shi ge.Recueil de poésies morales

Antipyretic and anti-inflammatory properties of the ethanolic extract, dichloromethane fraction and costunolide from Magnolia ovata (Magnoliaceae)

Aim of the study: Magnolia ovata (A.St.-Hil.) Spreng (formerly Talauma ovata), known as ""pinha-do-brejo"" or ""baguacu"", is a large tree widely distributed in Brazil. Its trunk bark has been used in folk medicine against fever. However, no data have been published to support the antipyretic ethnopharmacological use. This study investigated the antipyretic and anti-inflammatory effects of the ethanolic extract (EEMO). dichloromethane fraction (DCM), and the isolated compound costunolide. Materials and methods: The antipyretic and anti-inflammatory activities were evaluated in experimental models of fever and inflammation in mice. Results: The oral administration of EEMO, DCM and costunolide inhibited carrageenan (Cg)-induced paw oedema (ID(50) 72.35 (38.64-135.46) mg/kg, 5.8 (2.41-14.04) mg/kg and 0.18 (0.12-0.27) mg/kg, respectively) and was effective in abolishing lipopolysaccharide (LPS)-induced fever (30 mg/kg, 4.5 mg/kg and 0.15 mg/kg, respectively). EEMO was also effective in reducing cell migration in the pleurisy model. Intraplantar injection of costunolide also reduced the paw oedema, myeloperoxidase and N-acetyl-glucosaminidase activity induced by Cg in mice. Conclusions: Collectively, these results show, for the first time, that extracts obtained from Magnolia ovata possess antipyretic and anti-inflammatory properties, and costunolide appears to be the compound responsible for these effects. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

Associa��ão de enterococcus faecalis, candida albicans e escherichia coli em canais radiculares e avalia��ão dos efeitos de extratos naturais sobre os microrganismos e endotoxinas

Coordena��ão de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)