Human atrial beta1L-adrenoceptor but not beta3-adrenoceptor activation increases force and Ca2+ current at physiological temperature

BACKGROUND AND PURPOSE It has been proposed that BRL37344, SR58611 and CGP12177 activate b3-adrenoceptors in human atrium to increase contractility and L-type Ca2+ current (ICa-L). b3-adrenoceptor agonists are potentially beneficial for the treatment of a variety of diseases but concomitant cardiostimulation would be potentially harmful. It has also been proposed that (-)-CGP12177 activates the low affinity binding site of the b1-adrenoceptor in human atrium. We therefore used BRL37344, SR58611 and (-)-CGP12177 with selective b-adrenoceptor subtype antagonists to clarify cardiostimulant b-adrenoceptor subtypes in human atrium. EXPERIMENTAL APPROACH Human right atrium was obtained from patients without heart failure undergoing coronary artery bypass or valve surgery. Cardiomyocytes were prepared to test BRL37344, SR58611 and CGP12177 effects on ICa-L. Contractile effects were determined on right atrial trabeculae. KEY RESULTS BRL37344 increased force which was antagonized by blockade of b1- and b2-adrenoceptors but not by blockade of b3-adrenoceptors with b3-adrenoceptor-selective L-748,337 (1 mM). The b3-adrenoceptor agonist SR58611 (1 nM–10 mM) did not affect atrial force. BRL37344 and SR58611 did not increase ICa-L at 37°C, but did at 24°C which was prevented by L-748,337. (-)-CGP12177 increased force and ICa-L at both 24°C and 37°C which was prevented by (-)-bupranolol (1–10 mM), but not L-748,337. CONCLUSIONS AND IMPLICATIONS We conclude that the inotropic responses to BRL37344 are mediated through b1- and b2-adrenoceptors. The inotropic and ICa-L responses to (-)-CGP12177 are mediated through the low affinity site b1L-adrenoceptor of the b1-adrenoceptor. b3-adrenoceptor-mediated increases in ICa-L are restricted to low temperatures. Human atrial b3-adrenoceptors do not change contractility and ICa-L at physiological temperature.

A cascaded discharge plasma-adsorbent technique for engine exhaust treatment

A cascaded system of electrical discharges (non-thermal plasma) and adsorption process was investigated for the removal of oxides of Nitrogen (NOx) and total hydrocarbons (THC) from an actual diesel engine exhaust. The non-thermal plasma and adsorption processes were separately studied first and then the cascaded process was studied. In this study, different types of adsorbents were used. The NOx removal efficiency was higher with plasma-associated adsorption (cascaded) process compared to the individual processes and the removal efficiency was found almost invariant in time. When associated by plasma, among the adsorbents studied, activated charcoal and MS-13X were more effective for NOx and THC removal respectively. The experiments were conducted at no load and at 50% load conditions. The plasma reactor was kept at room temperature throughout the experiment, while the temperature of the adsorbent reactor was varied. A relative comparison of adsorbents was discussed at the end.

希土D—葡萄糖酸配合物的红外和拉曼光谱及结构的研究

十五种希土D-葡萄糖酸配合物的红外和拉曼光谱非常相似,结构类同,配合物的糖酸谱带明显展宽,羧酸根的对称和反对称振动谱带相差200cm~(-1),与希土离子单齿配位,糖酸为β构型,吡喃环上的O_1和O_5也参予配位,希土离子配位数是9。

Sala polivalente de actividades lúdicas.

Se trata de dotar al centro de un espacio específico para fomentar las posibilidades expresivas del alumnado, a través del juego, la psicomotricidad y la dramatización. Sus objetivos son que los alumnos conozcan y controlen progresivamente su cuerpo, que se relacionen con los adultos y con los otros niños, y que expresen sentimientos y experiencias. La metodología se basa en el juego y se trabaja con juegos de atención, memoria, vocabulario e imitación, que hacen posible la transferencia de procedimientos, conceptos y actitudes entre las diferentes áreas curriculares. Desarrolla contenidos referidos a la direccionalidad del propio cuerpo, imitación de situaciones y personajes reales y evocados, y libre expresión con instrumentos musicales, sonido, ritmo y danza. La evaluación se basa en criterios como adquisición de capacidades motrices, cognitivo-lingüísticas, de relación con compañeros y adultos, y de equilibrio musical.

H-1-N-15 NMR studies of the complex bis(S-allyl-L-cysteinate)palladium(II)

H-1-N-15 2D NMR data for S-allyl-L-cysteine (deoxyalliin) and for bis(S-allyl-L-cysteinate)palladium(II) complex are presented in this manuscript. Large upfield N-15 NMR shift of the amine nitrogen in the spectrum of the complex when compared to the spectrum of the ligand shows clearly coordination of S-allyl-L-cysteine, in the anion form, to palladium(II) through the NH2 group. (c) 2005 Elsevier B.V. All rights reserved.

Análisis de Ruido en Reactores PWR

Esta tesis se ha llevado a cabo persiguiendo dos objetivos principales: uno de ellos es el desarrollo y la aplicación de modelos para el mantenimiento predictivo de sensores en centrales nucleares, y el otro es profundizar en el entendimiento de los fenómenos que tienen influencia en el ruido de la señal de los detectores de neutrones de los reactores de agua a presión con ayuda de herramientas de simulación 3D. Para el desarrollo de los trabajos se ha contado con medidas de ruido de reactores PWR actualmente en operación registradas en el curso de la tesis. El análisis de estas medidas ha permitido desarrollar los modelos de los sensores a partir de sus señales reales y comparar lo obtenido en las simulaciones con la realidad. El estudio de los sensores y la elaboración de los modelos se han llevado a cabo mediante la aplicación de técnicas autorregresivas a las señales tomadas en planta. Para la reproducción de los fenómenos que tienen lugar en el núcleo del reactor y que pueden influir en el ruido neutrónico se ha contado con códigos neutrónicos ampliamente utilizados en la industria y con modelos actualizados y validados de las plantas. ABSTRACT There are two goals in this thesis. The first one is the development of models and its application for predictive maintenance of sensors in nuclear power plants. The second one is to improve the understanding of the phenomena that influence the neutron noise in pressurized water reactors by using 3D simulators. Real plant measurements recorded during this thesis have been used to achieve such goals. The information provided by the data led the development of the models and the comparison of the results provided by the computational simulations. Sensor models were obtained by applying autorregresive techniques to the signals recorded in the plant. Wide known codes in the nuclear industry as well as updated and validated models have been used for the reproduction of the phenomena that take place in the core an may influence the neutron noise.

VanB-type Enterococcus faecium clinical isolate successively inducibly resistant to, dependent on, and constitutively resistant to vancomycin

Three Enterococcus faecium strains isolated successively from the same patient, vancomycin-resistant strain BM4659, vancomycin-dependent strain BM4660, and vancomycin-revertant strain BM4661, were indistinguishable by pulsed-field gel electrophoresis and harbored plasmid pIP846, which confers VanB-type resistance. The vancomycin dependence of strain BM4660 was due to mutation P(175)L, which suppressed the activity of the host Ddl D-Ala:D-Ala ligase. Reversion to resistance in strain BM4661 was due to a G-to-C transversion in the transcription terminator of the vanRS(B) operon that lowered the free energy of pairing from -13.08 to -6.65 kcal/mol, leading to low-level constitutive expression of the resistance genes from the P(RB) promoter, as indicated by analysis of peptidoglycan precursors and of VanX(B) D,D-dipeptidase activity. Transcription of the resistance genes, studied by Northern hybridization and reverse transcription, initiated from the P(YB) resistance promoter, was inducible in strains BM4659 and BM4660, whereas it started from the P(RB) regulatory promoter in strain BM4661, where it was superinducible. Strain BM4661 provides the first example of reversion to vancomycin resistance of a VanB-type dependent strain not due to a compensatory mutation in the ddl or vanS(B) gene. Instead, a mutation in the transcription terminator of the regulatory genes resulted in transcriptional readthrough of the resistance genes from the P(RB) promoter in the absence of vancomycin.