Desenvolvimento de métodos para quantificação de benzilpenicilina em medicamentos veterinários e seus resíduos em leite bovino e caprino por UFLC-DAD

The Benzylpenicillin (PENG) have been as the active ingredient in veterinary medicinal products, to increase productivity, due to its therapeutic properties. However, one of unfortunate quality and used indiscriminately, resulting in residues in foods exposed to human consumption, especially in milk that is essential to the diet of children and the ageing. Thus, it is indispensable to develop new methods able to detect this waste food, at levels that are toxic to human health, in order to contribute to the food security of consumers and collaborate with regulatory agencies in an efficient inspection. In this work, were developed methods for the quality control of veterinary drugs based on Benzylpenicillin (PENG) that are used in livestock production. Additionally, were validated methodologies for identifying and quantifying the antibiotic residues in milk bovine and caprine. For this, the analytical control was performed two steps. At first, the groups of samples of medicinal products I, II, III, IV and V, individually, were characterized by medium infrared spectroscopy (4000 – 600 cm-1). Besides, 37 samples, distributed in these groups, were analyzed by spectroscopy in the ultraviolet and near infrared region (UV VIS NIR) and Ultra Fast Liquid Chromatograph coupled to linear arrangement photodiodes (UFLC-DAD). The results of the characterization indicated similarities, between PENG and reference standard samples, primarily in regions of 1818 to 1724 cm-1 of ν C=O that shows primary amides features of PENG. The method by UFLC-DAD presented R on 0.9991. LOD of 7.384 × 10-4 μg mL-1. LOQ of 2.049 × 10-3 μg mL-1. The analysis shows that 62.16% the samples presented purity ≥ 81.21%. The method by spectroscopy in the UV VIS NIR presented medium error ≤ 8 – 12% between the reference and experimental criteria, indicating is a secure choice for rapid determination of PENG. In the second stage, was acquiring a method for the extraction and isolation of PENG by the addition of buffer McIlvaine, used for precipitation of proteins total, at pH 4.0. The results showed excellent recovery values PENG, being close to 92.05% of samples of bovine milk (method 1). While samples of milk goats (method 2) the recovery of PENG were 95.83%. The methods for UFLC-DAD have been validated in accordance with the maximum residue limit (LMR) of 4 μg Kg-1 standardized by CAC/GL16. Validation of the method 1 indicated R by 0.9975. LOD of 7.246 × 10-4 μg mL-1. LOQ de 2.196 × 10-3 μg mL-1. The application of the method 1 showed that 12% the samples presented concentration of residues of PENG > LMR. The method 2 indicated R by 0.9995. LOD 8.251 × 10-4 μg mL-1. LOQ de 2.5270 × 10-3 μg mL-1. The application of the method showed that 15% of the samples were above the tolerable. The comparative analysis between the methods pointed better validation for LCP samples, because the reduction of the matrix effect, on this account the tcalculs < ttable, caused by the increase of recovery of the PENG. In this mode, all the operations developed to deliver simplicity, speed, selectivity, reduced analysis time and reagent use and toxic solvents, particularly if compared to the established methodologies.

O processo de subjetivação profissional durante os estágios supervisionados em psicologia

Internship is legally understood in Brazil as an overseen educational activity to be developed in a work setting aiming to prepare students to make productive work. According to the federal law number 11.788/08, its main aim is to provide students with competences directly related to the professional activity in a curricular context and to make students develop themselves to live as citizens and to work professionally. With a focus on the professionalizing internship, this research aimed at understanding the professional subjectiveness process of Psychology undergraduates at a public university in the state Minas Gerais during their internship and according to a critic perspective in schooling psychology. The importance of studying psychologist academic training above all the relationship between theory and practice is on the fact that the internship is his/her initial contact with the professional experience itself after four years of theoretical studies. Understood as a way of appropriating and internalizing theoretical and practical dimensions related to the psychologist profession and constructed by the individual, the concept of professional subjectiveness is viewed in the light of the cultural-historical theory, that is, as a result of the human being social constitution. Three Psychology s intern students and their respective overseers were interviewed during their internship period to talk about the process of professional constitution at this phase of the academic training. To do so, oral history proved to be an important methodological support, for it offers a means of giving voice to common people and letting them signify their multi-circumstantial experiences. Results reiterate the presence of subjectivity in the dynamics of an undergraduate training, but they showed that it is permeated by social relations established in his/her personal life story and professional trajectory, stemming from the professional choice, the relationship between intern and overseer, the professional activity properly considered, the psychotherapy, the rules, and from the institutionalization of knowledge, among others aspects that guide the professional subjectiveness during the internship. We verified that the training of a Psychology undergraduate includes multiple determinations that exceed the academic setting because it points out the breaking down of the professional dimensions and personal one dichotomy.

A psicologia e o psicólogo do esporte : uma formação necessária

Dissertação (mestrado)—Universidade de Brasília, Instituto de Psicologia, Programa de Pós-graduação em Processos de Desenvolvimento Humano e Saúde, 2016.

Trajetória profissional de professoras do ensino médio : sentidos e usos de tecnologias digitais na sala de aula

Tese (doutorado)—Universidade de Brasília, Instituto de Psicologia, Programa de Pós-Graduação em Processos de Desenvolvimento Humano e Saúde, 2016.