Dinâmica locacional das indústrias e a produção do espaço urbano em Poços de Caldas (MG)

Pós-graduação em Geografia - IGCE

Interação núcleo-citoplasmática em embriões e expressão de genes imprinted em fetos bovinos produzidos in vivo, in vitro e partenogenéticos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Polímeros impressos com íons: fundamentos, estratégias de preparo e aplicações em química analítica

Chemical imprinting technology has been widely used as a valuable tool in selective recognition of a given target analyte (molecule or metal ion), yielding a notable advance in the development of new analytical protocols. Since their discovery, molecularly imprinted polymers (MIPs) have been extensively studied with excellent reviews published. However, studies involving ion imprinted polymers (IIPs), in which metal ions are recognized in the presence of closely related inorganic ions, remain scarce. Thus, this review involved a survey of different synthetic approaches for preparing ion imprinted adsorbents and their application for the development of solid phase extraction methods, metal ion sensors (electrodes and optodes) and selective membranes.

Indole-3-carbinol attenuates the deleterious gestational effects of bisphenol A exposure on the prostate gland of male F1 rats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Stable, mobile, dark-in-bright, dipolar Bose-Einstein-condensate solitons

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Stable and mobile excited two-dimensional dipolar Bose-Einstein condensate solitons

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Os papéis dos RNAs não codificantes no desenvolvimento embrionário: um panorama geral

The genome of multicellular organisms shows a ruge index of RNA transcripts that are not protein coding. These ncRNAs act in housekeeping and genic regulation, as well in signaling and cell differentiation, crucial events to embryonic and ontogenetic development. Moreover, another events require the orderly expression these transcripts, as in cromossomic inactivation and genomic imprinting process, and their fail may cause several syndromes, malformations, illness and even death of affected individual. This review focus is to present the main acting pathways of ncRNAs already studied, as well to introduce the actual landscape of Dapper gene cluster and its performance in vertebrate development.

Memória de onde, memória de quem

The spatial formation of Rio Claro city preserves several features of the problematic transition from the slave to remunerated labor in the late 20th, when the tacit ethnic bipolar sociability standard emerged, imprinting “black” and “white” forms to the city’s urban space. The ethnic social clubs José do Patrocínio and Tamoio, are two objects of ethnic groups’ exclusive use. Such ethnic social clubs indicate the existence of conflicts in their formation, as well as in their present existence, they are forms that preserve in themselves the codes and histories of the local black movement’s fight for their rights and for an autonomous place in the local society. In the present context the spaces are increasingly subjected to commercial interests and the spirits are embedded in confusion. What is the value of these spaces for the subjects who built them? The versions and narratives from oral accounts will allow us to understand the existing dimensions in the relation between the places and the memory, evidencing the importance of the patrimony and the patrimonial policies to the cities in the present historical period of geographic space globalization

A influência da música nas atividades físicas e sua impressão no corpo e nos estados de ânimo

Numerous studies in physical education and music's area have been conducted to improve the quality of life and health of people, all of them under physiological, psychological and social fields. Thus, when properly analyzed, both Areas are connected, because the movement itself, main subject of physical education, has a close proximity to the rhythm, an essential element of music. That's why it is important to discover the benefits of this relationship, because, in many of its practices, physical education makes use of music, for example, in aerobic gymnastic and dance classes. As a consequence, this study aims to investigate, through literature, the influence of music in physical activities and its imprinting in the states of mind and body of practitioners, as well as clarify the criteria for choice of songs that are used in such practice. This research occurred under literary explore, done through theoretical analysis of the thematic. At first place, a contextualization of the auditory system and its relationship with the music and the perception of sound were made, then the main theme was explored. The music, even before generate and influence motor and psychic movements in the human body, is already moving in space, because it is formed through vibrations of molecules. It is decoded first as a movement from the ears, and then decoded as sound, when in the brain. It is important to worry about sounds at high intensities and also with those which are outside the hearing thresholds within physical activities involving music, because they may be harmful to health and also to states of mind. As far as music and movement is concerned, they are closely linked, and the human being has an instinctive sense of rhythm that can be seen when listening to music, because the motor cortex is also activated. Therefore, it is important to work the sound and body movement together, because in practice they are inseparable and they contribute greatly to...

Imprinted gene expression in in vivo- And in vi/ro-produced bovine embryos and chorio-allantoic membranes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desenvolvimento de sistema de análise para valerato de estradiol baseado em polímeros biomiméticos

Abstracts : The development of analytic methods more selective and sensitive is of great importance for a better quality in the determination of chemical species, therefore increasing the reliability of the results. In this way, the optimization of separation/concentration is still necessary. The use of Molecularly Imprinted Polymers - MIPs have demonstrated to be an efficient tool of analysis with a great potential in minimizing limitations of separation/concentration techniques traditionally employed. In general, the MIPs are obtained by polymerization in the presence of a template to be imprinted so that a polymeric skeleton is formed around the future analyte. In the present work, the template used is Estradiol Valerate (EV), compound used in the hormone replacement therapy (HRT) during climacteric. After the polymerization in bulk and in an anaerobic environment using MAA, EGDMA, AIBN, acetonitrile and VE, the obtained MIP was powdered, sifted (<120 μm) and placed in a soxhlet system containing ethanol at 60 °C, in order to remove the imprinted molecule through six successive washes in periods of 24 hours. The water used in the washings was analyzed using HPLC and spectrophotometry UV/Vis. Then, the obtained MIP was dried at room temperature and 150 mg was inset in SPE cartridges in order to evaluate the polymer's efficiency in the analyte pre-concentration and extraction. To do so, 100,0 mL of VE standard solution (2mg L-1) were pre-concentrated at 4,0 mL min-1 and eluted with 10,0 mL ethanol at 1,0 mL min-1, obtaining recoveries of 53%. Additionally, a NIP (non-imprinting polymer) was prepared to compare the obtained results, in which the recovery was 80%. In the same way, studies were conducted using commercial Strata™-X cartridges, obtaining 53% recovery. Since, the results did not reflect that than was expected, in relation with the MIP efficiency in the recovery, a computational ...

Detecção e quantificação de corantes de importância ambiental empregando sensores químicos baseados em fibras ópticas e polímeros molecularmente impressos

Pós-graduação em Química - IQ

Nanoassisted Laser Desorption-Ionization-MS Imaging of Tumors

The ability of nanoassisted laser desorption-ionization mass spectrometry (NALDI-MS) imaging to provide selective chemical monitoring with proper spatial distribution of lipid profiles from tumor tissues after plate imprinting has been tested. NALDI-MS imaging identified and mapped several potential lipid biomarkers in a murine model of melanoma tumor (inoculation of B16/F10 cells). It also confirmed that the in vivo treatment of tumor bearing mice with synthetic supplement containing phosphoethanolamine (PHO-S) promoted an accentuated decrease in relative abundance of the tumor biomarkers. NALDI-MS imaging is a matrix-free LDI protocol based on the selective imprinting of lipids in the NALDI plate followed by the removal of the tissue. It therefore provides good quality and selective chemical images with preservation of spatial distribution and less interference from tissue material. The test case described herein illustrates the potential of chemically selective NALDI-MS imaging for biomarker discovery.

Developmental and Epigenetic Anomalies in Cloned Cattle

Many of the developmental anomalies observed in cloned animals are related to foetal and placental overgrowth, a phenomenon known as the 'large offspring syndrome' (LOS) in ruminants. It has been hypothesized that the epigenetic control of imprinted genes, that is, genes that are expressed in a parental-specific manner, is at the root of LOS. Our recent research has focused on understanding epigenetic alterations to imprinted genes that are associated with assisted reproductive technologies (ART), such as early embryo in vitro culture (IVC) and somatic cell nuclear transfer (SCNT) in cattle. We have sought and identified single nucleotide polymorphisms in Bos indicus DNA useful for the analysis of parental-specific alleles and their respective transcripts in tissues from hybrid embryos derived by crossing Bos indicus and Bos taurus cattle. By analysing differentially methylated regions (DMRs) of imprinted genes SNRPN, H19 and the IGF2R in cattle, we demonstrated that there is a generalized hypomethylation of the imprinted allele and the biallelic expression of embryos produced by SCNT when compared to the methylation patterns observed in vivo (artificially inseminated). Together, these results indicate that imprinting marks are erased during the reprogramming of the somatic cell nucleus during early development, indicating that such epigenetic anomalies may play a key role in mortality and morbidity of cloned animals.

1031-1034delTAAC (Leu125Stop): a novel familial UBE3A mutation causing Angelman syndrome in two siblings showing distinct phenotypes

Background More than 50 mutations in the UBE3A gene (E6-AP ubiquitin protein ligase gene) have been found in Angelman syndrome patients with no deletion, no uniparental disomy, and no imprinting defect. Case Presentation We here describe a novel UBE3A frameshift mutation in two siblings who have inherited it from their asymptomatic mother. Despite carrying the same UBE3A mutation, the proband shows a more severe phenotype whereas his sister shows a milder phenotype presenting the typical AS features. Conclusions We hypothesized that the mutation Leu125Stop causes both severe and milder phenotypes. Potential mechanisms include: i) maybe the proband has an additional problem (genetic or environmental) besides the UBE3A mutation; ii) since the two siblings have different fathers, the UBE3A mutation is interacting with a different genetic variant in the proband that, by itself, does not cause problems but in combination with the UBE3A mutation causes the severe phenotype; iii) this UBE3A mutation alone can cause either typical AS or the severe clinical picture seen in the proband.