Nanoassisted Laser Desorption-Ionization-MS Imaging of Tumors
| Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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| Data(s) |
05/11/2013
05/11/2013
2012
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| Resumo |
The ability of nanoassisted laser desorption-ionization mass spectrometry (NALDI-MS) imaging to provide selective chemical monitoring with proper spatial distribution of lipid profiles from tumor tissues after plate imprinting has been tested. NALDI-MS imaging identified and mapped several potential lipid biomarkers in a murine model of melanoma tumor (inoculation of B16/F10 cells). It also confirmed that the in vivo treatment of tumor bearing mice with synthetic supplement containing phosphoethanolamine (PHO-S) promoted an accentuated decrease in relative abundance of the tumor biomarkers. NALDI-MS imaging is a matrix-free LDI protocol based on the selective imprinting of lipids in the NALDI plate followed by the removal of the tissue. It therefore provides good quality and selective chemical images with preservation of spatial distribution and less interference from tissue material. The test case described herein illustrates the potential of chemically selective NALDI-MS imaging for biomarker discovery. FAPESP FAPESP CNPq CNPq CAPES CAPES |
| Identificador |
ANALYTICAL CHEMISTRY, WASHINGTON, v. 84, n. 15, supl. 2, Part 3, pp. 6341-6345, AUG 7, 2012 0003-2700 http://www.producao.usp.br/handle/BDPI/41418 10.1021/ac301202q |
| Idioma(s) |
eng |
| Publicador |
AMER CHEMICAL SOC WASHINGTON |
| Relação |
ANALYTICAL CHEMISTRY |
| Direitos |
closedAccess Copyright AMER CHEMICAL SOC |
| Palavras-Chave | #MALDI-TOF-MS #MASS-SPECTROMETRY #ELECTROSPRAY-IONIZATION #CARBON NANOTUBES #POROUS SILICON #DESORPTION/IONIZATION #TISSUES #MATRIX #CANCER #BRAIN #CHEMISTRY, ANALYTICAL |
| Tipo |
article original article publishedVersion |
