Identification and classification of p53-regulated genes

Sequence-specific transactivation by p53 is essential to its role as a tumor suppressor. A modified tetracycline-inducible system was established to search for transcripts that were activated soon after p53 induction. Among 9,954 unique transcripts identified by serial analysis of gene expression, 34 were increased more than 10-fold; 31 of these had not previously been known to be regulated by p53. The transcription patterns of these genes, as well as previously described p53-regulated genes, were evaluated and classified in a panel of widely studied colorectal cancer cell lines. “Class I” genes were uniformly induced by p53 in all cell lines; “class II” genes were induced in a subset of the lines; and “class III” genes were not induced in any of the lines. These genes were also distinguished by the timing of their induction, their induction by clinically relevant chemotherapeutic agents, the absolute requirement for p53 in this induction, and their inducibility by p73, a p53 homolog. The results revealed substantial heterogeneity in the transcriptional responses to p53, even in cells derived from a single epithelial cell type, and pave the way to a deeper understanding of p53 tumor suppressor action.

ICB database: the gyrB database for identification and classification of bacteria

The Identification and Classification of Bacteria (ICB) database (http:/www.mbio.co.jp/icb) contains currently available information about the DNA gyrase subunit B (gyrB) gene in bacteria. The database is designed to provide the scientific community with a reference point for using gyrB as an evolutionary and taxonomic marker. Nucleic and amino acid sequence data are currently available for over 850 strains, along with alignments at several different taxonomic levels and an exhaustive review of primer selection and background information.

Lithological studies : a description and classification of the rocks of the Cordilleras / by M.E. Wadsworth. With eight plates.

v.11:no.1 (1884)

Sample information and classification of lower Miocene gravity-flow types of sediment core CRP-1 (Table 1)

Nineteen samples of the Cape Roberts-1 drillcore were taken from Miocene- age deposits, from 90.25 - 146.50 metres below seafloor (mbsf) for thin section and laser grain-size analysis. Using the grain-size distribution, detailed core logging, X-radiography and thin-section analysis of microstructures, coupled with a statistical grouping of the grain-size data, three main styles of gravity-flow sedimentation were revealed. Thin (centimetre-scale) muddy debris-flow deposits are the most common and are possibly tirggered by debris rain-out from sea-ice These deposits are characterised by very poorly sorted, faintly laminated muddy sandstones with coarse granules toward their base. Contacts are gradational to sharp. Variations on this style of mass-wasting deposit are rhythmically stacked sequences of pebbly-coarse sandstones representing successive thin debris-flow events. These suggest very high sedimentation rates on an unstable slope in a shallow-water proximal glacimarine environment. Sandy-silty turbidites appear more common in the lower sections of the core, below approximately 141.00 mbsf, although they occur occasionally with the debris flow deposits The turbidites are characterised by inversely to normally graded, well-laminated siltstones with occasional lonestones, and represent a more distal shallow-water glacimarine environment.

Evaluation of freeway high occupancy vehicle lanes and ramp metering. Volume I. Final report.

Transportation Department, Office of the Assistant Secretary for Policy and International Affairs, Washington, D.C.

Evaluation of freeway high occupancy vehicle lanes and ramp metering. Volume II: bibliography and abstracts.

Transportation Department, Office of the Assistant Secretary for Policy and International Affairs, Washington, D.C.

Improved commercial vehicle conspicuity and signalling systems. Task I: accident analysis and functional requirements. Interim report.

National Highway Traffic Safety Administration, Washington, D.C.

Improved commercial vehicle conspicuity and signalling systems. Task II: analyses, experiments and design recommendations. Interim report.

National Highway Traffic Safety Administration, Washington, D.C.

Vehicle integration and evaluation of advanced restraint systems. Phase B - test report: Torino-to-Volvo tests.

National Highway Traffic Safety Administration, Washington, D.C.

Vehicle handling and stability.

Mode of access: Internet.

The energy and environmental consequences of increased vehicle size and weight - volume I - final report.

Transportation Department, Research and Special Programs Administration, Washington, D.C.

Motor vehicle defects and recall campaigns.

Mode of access: Internet.

Characteristics of vehicle repair and maintenance problems for current vehicle-in-use. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Vehicles-in-use sub-limit maneuvers. Volume III: effects of vehicle-in-use spring sag on vehicle handling and braking. Final report.

National Highway Traffic Safety Administration, Vehicle Engineering Research Division, Washington, D.C.