Urinary analysis of 16(5alpha)-androsten-3alpha-ol by gas chromatography/combustion/isotope ratio mass spectrometry: implications in anti-doping analysis.

We present a method for the analysis of urinary 16(5alpha)-androsten-3alpha-ol together with 5beta-pregnane-3alpha,20alpha-diol and four testosterone metabolites: androsterone (Andro), etiocholanolone (Etio), 5alpha-androstane-3alpha,17beta-diol (5alphaA), 5beta-androstane-3alpha,17beta-diol (5betaA) by means of gas chromatography/combustion/isotopic ratio mass spectrometry (GC/C/IRMS). The within-assay and between-assay precision S.D.s of the investigated steroids were lower than 0.3 and 0.6 per thousand, respectively. A comparative study on a population composed of 20 subjects has shown that the differences of the intra-individual delta(13)C-values for 16(5alpha)-androsten-3alpha-ol and 5beta-pregnane-3alpha,20alpha-diol are less than 0.9 per thousand. Thereafter, the method has been applied in the frame of an excretion study following oral ingestion of 50 mg DHEA initially and oral ingestion of 50mg pregnenolone 48 h later. Our findings show that administration of DHEA does not affect the isotopic ratio values of 16(5alpha)-androsten-3alpha-ol and 5beta-pregnane-3alpha,20alpha-diol, whereas the isotopic ratio values of 5beta-pregnane-3alpha,20alpha-diol vary by more 5 per thousand upon ingestion of pregnenolone. We have observed delta(13)C-value changes lower than 1 per thousand for 16(5alpha)-androsten-3alpha-ol, though pregnenolone is a precursor of the 16-ene steroids. In contrast to 5beta-pregnane-3alpha,20alpha-diol, the 16-ene steroid may be used as an endogenous reference compound when pregnenolone is administered.

Réclamations pour le Sr Charles-Marie-Canalès-Oglou,... seul et unique héritier de feu Sr Jean-Marie-Alix de Boullon-Morange,... demandeur, contre dame Marie-Elisabeth-Emilie Aubourg, veuve du sieur Léonard Robin, décédé commissaire du gouvernement près le Tribunal civil... de la Seine, et membre du Tribunat, et encore contre le sieur Louis-Léonard Robin, se prétendant fils et unique héritier dudit sieur Léonard Robin... défendeurs, adressées à S. M. Napoléon-le-Grand,... / [Signé : Canalès-Oglou, 12 août 1806. - Consultation pour le sieur Canalès-Oglou contre les héritiers Robin. Signé : Adam, ancien avocat ; Bonhomme, avocat, 16 août 1806.]

[Factum. Boullon-Morange, Mehemet-Aly, dit Jean-Marie-Alix deSuccession). 1806?]

[16 phot. de Saint-Gervais et de la région du Mont-Blanc par Numa Allantaz, phot. à Saint-Gervais, don J. Jackson en 1885]

Donateur : Jackson, James (1843-1895)

16 días de activismo contra la violencia de género: pintando de naranja nuestro mundo

Boletín semanal para profesionales sanitarios de la Secretaría General de Calidad, Innovación y Salud Pública de la Consejería de Igualdad, Salud y Políticas Sociales

Genomic acute myeloid leukemia-associated inv(16)(p13q22) breakpoints are tightly clustered.

The inv(16) and related t(16;16) are found in 10% of all cases with de novo acute myeloid leukemia. In these rearrangements the core binding factor beta (CBFB) gene on 16q22 is fused to the smooth muscle myosin heavy chain gene (MYH11) on 16p13. To gain insight into the mechanisms causing the inv(16) we have analysed 24 genomic CBFB-MYH11 breakpoints. All breakpoints in CBFB are located in a 15-Kb intron. More than 50% of the sequenced 6.2 Kb of this intron consists of human repetitive elements. Twenty-one of the 24 breakpoints in MYH11 are located in a 370-bp intron. The remaining three breakpoints in MYH11 are located more upstream. The localization of three breakpoints adjacent to a V(D)J recombinase signal sequence in MYH11 suggests a V(D)J recombinase-mediated rearrangement in these cases. V(D)J recombinase-associated characteristics (small nucleotide deletions and insertions of random nucleotides) were detected in six other cases. CBFB and MYH11 duplications were detected in four of six cases tested.

[Panneaux électoraux dans la rue pour les élections législatives du 16 novembre 1919 sous la neige] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 56915

13/11/19, affiches électorales [panneaux électoraux dans la rue pour les élections législatives du 16 novembre 1919] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 56909