Estudo e desenvolvimento de modelo de dispositivo controlado por temperatura no contexto de eficiência energética: potencial de aplicação para DSR-SF

Dissertação para a obtenção do grau de Mestre em Engenharia Electrotécnica Ramo de Energia

N-acétyltransférase lysosomale : organisation, fonctionnement et défauts moléculaires chez les patients atteints du syndrome de Sanfilippo type C

L’acétylation des résidus de glucosamine terminaux par la N-acétyltransférase lysosomale (HGSNAT) est une étape essentielle de la dégradation catabolique de l’héparan sulfate. Des défauts dans cette réaction causent une maladie de surcharge lysosomale autosomale récessive rare : le désordre de Sanfilippo type C (SFC). À ce jour, 54 mutations ont été rapportées chez des patients SFC, incluant 13 mutations des sites d’épissage, 11 insertions et délétions, 8 mutations non-sens, 18 mutations faux-sens et 4 polymorphismes, avec différentes manifestations phénotypiques. Nous avons identifié 10 d’entre elles et effectué une étude exhaustive portant sur l’éventail des mutations SFC, leur distribution dans la population de patients, ainsi que leur impact potentiel sur la structure de la HGSNAT. Les erreurs d’épissage, les mutations non-sens, les insertions et les délétions devraient toutes entraîner un ARN non fonctionnel qui est rapidement dégradé par des mécanismes de contrôle qualité cellulaire. Les 4 polymorphismes identifiés sont des changements d'acides aminés qui ne modifient pas l'activité enzymatique, la glycosylation ou la localisation et n'ont donc pas de signification au niveau clinique. Au niveau des enzymes, les polymorphismes sont des changements d’acides aminés qui n’affectent pas la fonction, mais dans un contexte d’acides nucléiques ils peuvent être considérés comme des mutations faux-sens. Les dix-huit mutations faux-sens qui ont été exprimées ont produit des protéines inactives, en raison d'erreurs dans leur repliement. Ceci expliquerait donc la progression sévère de la maladie chez les personnes porteuses de ces mutations. Les protéines mutantes mal repliées sont anormalement glycosylées et conservées dans le réticulum endoplasmique. La thérapie par amélioration de l’activité enzymatique par des chaperonnes est une option thérapeutique potentielle, spécifiquement conçue pour exploiter l'activité enzymatique résiduelle de mutants mal repliés, afin d’éliminer les substrats stockés. Nous avons démontré que le traitement de plusieurs lignées de fibroblastes de patients SFC avec le chlorhydrate de glucosamine, un inhibiteur spécifique de la HGSNAT, a partiellement restauré l’activité de l'enzyme mutante, fournissant une preuve de l’utilité future de la thérapie par des chaperonnes dans le traitement de la maladie de SFC.

Le syndrome de fatigue chronique : méconnu mais pas sans solution

Travail d'intégration réalisé dans le cadre du cours PHT-6113.

Perdurabilidad y competitividad en bancos comerciales segmento masivo, tamaño intermedio: caso Colpatria

Teniendo en cuenta la complejidad del sistema financiero colombiano y su alto impacto en la economía nacional, surge el interés de realizar un estudio y análisis cualitativo y cuantitativo de diversos agentes de este sector. A partir de allí, se genera un diagnóstico de morbilidad, hacinamiento e imitación en el sector, así como de perdurabilidad del Banco Colpatria Durante el documento se hará énfasis en la estrategia de redes y alianzas del sector estudiado, el cual, según revisión, promueve la bancarización y acceso al sistema financiero en todo el país. A su vez, se analizarán indicadores financieros del sector, se utilizará análisis retrospectivo y se realizarán entrevistas y encuestas a los colaboradores del Banco Colpatria y a los usuarios de sus redes, con el propósito de entender el estado actual del sector, de la organización estudiada, así como de identificar características o elementos del pasado, presente y futuro de la banca y de sus agentes. Por lo tanto, se emite un diagnóstico del sector estratégico: bancos comerciales segmento masivo, tamaño intermedio y finalmente se generan recomendaciones para evitar el hacinamiento del sector y generar perdurabilidad en la entidad estudiada.

Hydrogen peroxide is a novel mediator of inflammatory hyperalgesia, acting via transient receptor potential vanilloid 1-dependent and independent mechanisms

Inflammatory diseases associated with pain are often difficult to treat in the clinic due to insufficient understanding of the nociceptive pathways involved. Recently, there has been considerable interest in the role of reactive oxygen species (ROS) in inflammatory disease, but little is known of the role of hydrogen peroxide (H(2)O(2)) in hyperalgesia. In the present study, intraplantar injection of H(2)O(2)-induced a significant dose- and time-dependent mechanical and thermal hyperalgesia in the mouse hind paw, with increased c-fos activity observed in the dorsal horn of the spinal cord. H(2)O(2) also induced significant nociceptive behavior Such as increased paw licking and decreased body liftings. H(2)O(2) levels were significantly raised in the carrageenan-induced hind paw inflammation model, showing that this ROS is produced endogenously in a model of inflammation. Moreover, superoxide dismutase and catalase significantly reduced carrageenan-induced mechanical and thermal hyperalgesia, providing evidence of a functionally significant endogenous role. Thermal, but not mechanical, hyperalgesia in response to H(2)O(2) (i.pl.) Was longer lasting in TRPV1 wild type mice compared to TRPV1 knockouts. It is unlikely that downstream lipid peroxidation was increased by H(2)O(2). In conclusion, we demonstrate a notable effect of H(2)O(2) in mediating inflammatory hyperalgesia, thus highlighting H(2)O(2) removal as a novel therapeutic target for anti-hyperalgesic drugs in the clinic. (C) 2008 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.

Neoproterozoic glacial dynamics revealed by provenance of diamictites of the Bebedouro Formation, Sao Francisco Craton, Central Eastern Brazil

One of the main questions on Neoproterozoic geology regards the extent and dynamics of the glacial systems that are recorded in all continents. We present evidence for short transport distances and localized sediment sources for the Bebedouro Formation, which records Neoproterozoic glaciomarine sedimentation in the central-eastern Sao Francisco Craton (SFC), Brazil. New data are presented on clast composition, based on point counting in thin section and SHRIMP dating of pebbles and detrital zircon. Cluster analysis of clast compositional data revealed a pronounced spatial variability of clast composition on diamictite indicating the presence of individual glaciers or ice streams feeding the basin. Detrital zircon ages reveal distinct populations of Archean and Palaeoproterozoic age. The youngest detrital zircon dated at 874 +/- 9 Ma constrains the maximum depositional age of these diamictites. We interpret the provenance of the glacial diamictites to be restricted to sources inside the SFC, suggesting deposition in an environment similar to ice streams from modern, high latitude glaciers.

Política e burocracia no Brasil: o controle sobre a execução das políticas públicas

Esta tese é um estudo de caso sobre a criação da Secretaria Federal de Controle Interno (SFC) do Executivo federal e das suas atividades como órgão de monitoramento da execução das políticas públicas. Através da análise de depoimentos e de documentos oficiais foi possível demonstrar que: a) a atividade de monitoramento sobre a execução das políticas federais existe, b) que ela é desempenhada por um órgão ¿ a SFC - criado recentemente e que é fruto de uma ampla reforma do sistema de controle interno do Executivo federal, e c) que essa atividade representa um recurso de poder do governo federal para controlar a direção da burocracia federal e a distribuição de poder na coalizão de governo. As principais contribuições desta tese são: 1) demonstrou como e em quais condições o monitoramento das políticas públicas se caracteriza como uma das formas do controle político sobre a burocracia, 2) analisou a construção institucional da SFC enquanto um recurso de poder do governo federal em face da burocracia e da coalizão de governo, 3) realizou um estudo inédito do órgão de monitoramento da execução das políticas públicas no Executivo federal, e 4) apontou os desdobramentos da atuação da SFC do ponto de vista do funcionamento do sistema político, levantando novas hipóteses e perspectivas de trabalho.

Efeito do hormônio hiperglicemiante de crustáceos sobre as reservas de carboidratos em Chasmagnathus granulata alimentados com uma dieta rica em proteínas ou em carboidratos

Estudos com o hormônio hiperglicemiante de crustáceos (CHH) tem sido realizados desde 1944 quando foi constatado, pela primeira vez, seu efeito na mobilização das reservas de glicogênio causando hiperglicemia. O objetivo deste trabalho foi investigar a ação do CHH sobre o metabolismo de carboidratos de caranguejos Chasmagnathus granulata intactos submetidos às dietas rica em proteínas (HP) ou rica em carboidratos (HC) e injetados com extrato de pedúnculos proveniente de animais de ambas as dietas. Após a injeção de extrato de pedúnculos, foram avaliados, in vivo: a glicose hemolinfática, a glicose livre hepatopancreática e muscular e o glicogênio hepatopancreático e muscular de animais alimentados com dieta HP, injetados com extrato de pedúnculos provenientes de animais HP (HP/HP), e de animais HC (HP/HC), bem como de animais alimentados com uma dieta HC, injetados com extrato de pedúnculos oriundos de animais HP (HC/HP) e de animais HC (HC/HC). Além de animais controle injetados com solução fisiológica para caranguejo (SFC). Os resultados obtidos confirmam a ação hiperglicemiante dos extratos de pedúnculos, independentemente da dieta de origem dos mesmos. Foi observado um padrão de resposta diferencial segundo a dieta a qual os animais foram submetidos. Assim, os resultados demonstram que nos animais submetidos a uma dieta HP o CHH agiu aumentando os níveis de glicose circulante a partir das reservas de glicogênio dos hepatopancreócitos e do músculo. Mas, nos animais alimentados com uma dieta HC, embora o CHH tenha elevado o nível de glicose circulante, não foram observadas alterações nas reservas de carboidratos do hepatopâncreas e do músculo. Estes resultados demonstram que o mecanismo de ação do CHH sobre as reservas de carboidratos de Chasmagnathus granulata depende da dieta a qual os animais foram submetidos.

U Pb and Sm Nd geochronologic studies of the eastern Borborema Province, Northeastern Brazil: initial conclusions

The Borborema Province of NE Brasil comprises the central part of a wide Pan-African-Brasiliano orogenetic belt that formed as a consequence of late Neoproterozoic convergence and collision of the São Luis-West Africa craton and the São Francisco-Congo-Kasai cratons. New Sm Nd and U Pb results from the eastern part of this province help to define the basic internal architecture and pre-collisional history of this province, with particular emphasis on delineating older cratonic terranes, their fragmentation during the Mesoproterozoic, and their assembly into West Gondwana during the Pan African-Brasiliano orogeny at ca. 600 Ma. The region can be divided into three major geotectonic domains: a) Rio Piranhas-Caldas Brandão massif, with overlying Paleoproterozoic to Neoproterozoic supracrustal rocks, north of the Patos Lineament; b) the Archean to Paleoproterozoic São Francisco craton (SFC) to the south; and c) a complex domain of Paleoproterozoic to Archean basement blocks with several intervening Mesoproterozoic to Neoproterozoic fold belts in the center (south of Patos Lineament and north of SFC). The northern and central domains comprise the Borborema Province. Archean basement gneiss and Transamazonian granulite of northern SFC are exposed in the southern part of the central domain, underlying southern parts of the Sergipano fold belt. Basement in the Rio Piranhas massif appears to consist mostly of Transamazonian (2.1 to 2.2 Ga) gneissic rocks; Nd model ages (TDM) of ca. 2.6 Ga for 2.15 Ga gneisses indicate a substantial Archean component in the protoliths to these gneisses. The Caldas Brandão massif to the east yields both Transamazonian and Archean U Pb zircon and Nd (TDM) ages, indicating a complex architecture. Metasedimentary rocks of the Jucurutu Formation yield detrital zircons with original crystallization ages as young as 1.8 Ga, indicating that these rocks may be late Paleoproterozoic and correlate with other ca. 1.8 Ga cratonic supracrustal rocks in Brazil such as the Roraima Group and Espinhaço Group. Most metavolcanic and pre-Brasiliano granitic units of the Sergipano (SDS), Pajeú-Paraíba (SPP), Riacho Pontal (SRP), and Piancó-Alto Brígida (SPAB) fold belts in the central domain formed ~ 1.0 ± 0.1 Ga, based on U Pb ages of zircons. Nd model ages (TDM) for these same rocks, as well as Brasiliano granites intruded into them and large parts of the Pernambuco-Alagoas massif, are commonly 1.3-1.7 Ga, indicating that rocks of the fold belts were not wholly derived from either older (> 2.1 Ga) or juvenile (ca. 1.0 Ga) crust, but include mixtures of both components. A simple interpretation of Brasiliano granite genesis and the Nd data implies that there is no Transamazonian or Archean basement underlying large parts of these fold belts or of the Pernambuco-Alagoas massif. An exception is a belt of syenitic Brasiliano plutons (Syenitoid Line) and host gneisses between SPAB and SPP that clearly has a Transamazonian (or older) source. In addition, there are several smaller blocks of Archean to Transamazonian gneiss that can be defined within and among these fold belts. These blocks do not appear to constitute a continuous basement complex, but appear to be isolated older crustal fragments. Our data support a model in which ca. 1.0 Ga rifting was an important tectonic and crust-forming event along the northern edge of the São Francisco craton. Our data also show that significant parts of the Borborema Province are not remobilized Transamazonian to Archean crust, but that Mesoproterozoic crust is a major feature of the Province. There are several small remnants of older crust within the area dominated by Mesoproterozoic crust, suggesting that the rifting event created several small continental fragments that were later incorporated into the Brasiliano collisional orogen. We cannot at present determine if the Rio Piranhas-Caldas Brandão massifs and the older crustal blocks of the central domain were originally part of the São Francisco craton or whether some (or all) of them came from more exotic parts of the Proterozoic Earth. Finally, our data have not yet revealed any juvenile terranes of either Transamazonian or Brasiliano age. © 1995.

Microfinanzas dentro del contexto del sistema financiero colombiano

Incluye Bibliografía

Efeitos de diferentes concentrações de dextrose e lipopolissacarídeo no comportamento corrosivo da liga Ti-6Al-4V com superfície usinada e tratada com duplo ataque ácido

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência da adição de bioactive glass na liberação de íons do cimento de ionômero de vidro modificado por resina

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Continuous enzymatic interesterification of milkfat with soybean oil produces a highly spreadable product rich in polyunsaturated fatty acids

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeitos neuroprotetor e regenerativo do selante de fibrina derivado da peçonha de serpente combinado com células tronco mononucleares após reparo do nervo isquiático de ratos neonatos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)