Rendimento de grãos e componentes primários de rendimento de híbridos de girassol em campo novo de parecis, na segunda safra do verão de 2015.

RESUMO: O objetivo deste trabalho foi avaliar rendimento de grãos e componentes primários de rendimento de híbridos de gIrassol em Campo Novo do Parecis, na segunda safra do verão de 2015. O estudo foi realizado no campo experimental do Instituto Federal de Educação, Ciência e Tecnologia de Mato Grosso ? campus Campo Novo do Parecis, entre os meses de fevereiro e junho de 2015. Foram analisados 13 tratamentos (híbridos), em delineamento de blocos casualizados, com 4 repetições. Foram avaliadas as seguintes características: tamanho do capitulo, massa de capítulo, massa de aquênios por capítulo, índice de colheita, massa de mil aquênios e produtividade de aquênios. Os dados foram submetidos à análise de variância e ao teste de média Scott-Knott (p<0,05). O híbrido BRS G44 apresentou os melhores resultados para rendimento de grãos e seus componentes primários avaliados na segunda safra de verão de 2015, em Campo Novo do Parecis. Assim, este híbrido pode ser uma opção de cultivo na região.

Avaliação de genótipos de girassol (helianthus annuus l.) em Campo Novo Do Parecis - MT.

RESUMO: O presente trabalho teve como objetivo avaliar genótipos de girassol semeados em segunda safra no ano de 2014 em Campo Novo do Parecis ? MT, no campo experimental do Instituto Federal de Educação Ciência e Tecnologia de Mato Grosso. O delineamento experimental utilizado foi o de blocos casualizados, com 16 tratamentos (16 genótipos) e quatro repetições. As parcelas experimentais foram constituídas de 4 linhas com 6,5 m de comprimento, com espaçamento entrelinhas de 0,45 m, contendo área de 11,7 m², totalizando uma área de 748m². Foi utilizada a população de 45000 plantas por hectare. Os dados foram submetidos à análise de variância e ao teste Scott-Knott, a 5% de probabilidade. Para a massa de mil aquênios, os genótipos que se destacaram foram BRS 323, MG 360 e M734 enquanto que as os mais produtivos foram os genótipos MG 360, AGUARÁ 06, MG 305, AGUARÁ 04, CF 101, SYN 045, GNZ NEON, HELIO 251 e SYN 3950HO. ABSTRACT: This study aimed to evaluate genotypes of sunflower seeded second harvest in the year 2014 in Campus Campo Novo do Parecis, in the experimental field of the Instituto Federal de Educação Ciência e Tecnologia de Mato Grosso. The experimental design was a randomized block design with treatments 16 (16 genotypes) and four replications. The experimental plots consisted of four rows 6.5 m long with row spacing of 0.45 m, containing area of 11.7 m², totaling an area of 748 m². The population of 45000 plants per hectare is used. Data were subjected to analysis of variance and the Scott - Knott test at 5 % probability. For the mass of thousand achenes, genotypes that stood out were BRS 323, MG 360 and M734 while the most productive genotypes were the MG 360, AGUARÁ 06, MG 305, AGUARÁ 04, CF 101, SYN 045, GNZ NEON, HELIO 251 and SYN 3950HO.

Caracteres agronômicos de híbridos de girassol em Campo Novo do Parecis, na segunda safra de verão de 2015.

RESUMO: O objetivo deste trabalho foi avaliar características agronômicos durante o desenvolvimento de híbridos de girassol cultivados na região de Campo Novo do Parecis - MT. O ensaio foi instalado e conduzido,entre os meses de fevereiro ejunho de 2015, na área experimental do setor de produção do Instituto Federal de Educação, Ciência e Tecnologia de Mato Grosso - IFMT Campus Campo Novo do Parecis - MT, cujas coordenadas geográficas são latitude S 13°40'31" longitude O 57°53'31" e altitude média de 574 m. O solo predominante é Latossolo Vermelho distrófico típico. O delineamento experimental foi em blocos casualizados com 13 tratamentos (híbridos) e 4 repetições, totalizando um total de 52 parcelas. Foram avaliadas as seguintes características agronômicas do girassol: dias para o florescimento inicial, dias para a maturação fisiológica, altura de planta, curvatura do caule, número de plantas acamadas, número de plantas quebradas e produtividade de aquênios. Os dados foram submetidos à análise de variância e ao teste de média Scott-Knott (p<0,05). O híbrido BRS G44 apresentou híbrido BRS G44 apresentou bom rendimento, ciclo precoce e porte baixo, nas condições de segunda safra de verão em Campo Novo do Parecis (MT) . Assim, este híbrido se torna boa opção para o cultivo de girassol na região. ABSTRACT: The objective of this study was to evaluate agronomic characteristics for the development of sunflower hybrids grown in the region of Campo Novo do Parecis - MT. The experiment was carried out and conducted between the months of February to June 2015 in the experimental area of the production sector of the Instituto Federal de Educação, Ciência e Tecnologia de Mato Grosso, - IFMT, Campo Novo do Parecis - MT (latitude S 13°40'31" longitude W 57°53'31" and average altitude of 574 m). The predominant soil is Typic Tropudox. The experimental design was randomized blocks with 13 treatments (hybrids) and four repetitions, resulting in 52 plots. The sunflower agronomic traits evaluated were: days to initial flowering, days to physiological maturity, plant height, stem curvature, number of lodged plants, number of broken plants and achenes productivity. Data were subjected to analysis of variance and average test Scott-Knott (p<0.05). The hybrid BRS G44 showed good yield, early maturity and low height in second summer crop conditions in Campo Novo do Parecis (MT). Thus, this hybrid is a good option for sunflower cultivation in the region.

O (Novo) Regulamento Sanitário Internacional

Projeto de Pós-Graduação/Dissertação apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências Farmacêuticas

Qualidade do ar interior e os requisitos de ar novo numa unidade hospitalar

Dissertação apresentada à Universidade Fernando Pessoa como parte dos requisitos para obtenção do Grau de Mestre em Engenharia e Gestão Ambiental, ramo de Sistemas Industriais

De novo design and molecular assembly of a transmembrane diporphyrin-binding protein complex.

The de novo design of membrane proteins remains difficult despite recent advances in understanding the factors that drive membrane protein folding and association. We have designed a membrane protein PRIME (PoRphyrins In MEmbrane) that positions two non-natural iron diphenylporphyrins (Fe(III)DPP's) sufficiently close to provide a multicentered pathway for transmembrane electron transfer. Computational methods previously used for the design of multiporphyrin water-soluble helical proteins were extended to this membrane target. Four helices were arranged in a D(2)-symmetrical bundle to bind two Fe(II/III) diphenylporphyrins in a bis-His geometry further stabilized by second-shell hydrogen bonds. UV-vis absorbance, CD spectroscopy, analytical ultracentrifugation, redox potentiometry, and EPR demonstrate that PRIME binds the cofactor with high affinity and specificity in the expected geometry.

Telomere disruption results in non-random formation of de novo dicentric chromosomes involving acrocentric human chromosomes.

Genome rearrangement often produces chromosomes with two centromeres (dicentrics) that are inherently unstable because of bridge formation and breakage during cell division. However, mammalian dicentrics, and particularly those in humans, can be quite stable, usually because one centromere is functionally silenced. Molecular mechanisms of centromere inactivation are poorly understood since there are few systems to experimentally create dicentric human chromosomes. Here, we describe a human cell culture model that enriches for de novo dicentrics. We demonstrate that transient disruption of human telomere structure non-randomly produces dicentric fusions involving acrocentric chromosomes. The induced dicentrics vary in structure near fusion breakpoints and like naturally-occurring dicentrics, exhibit various inter-centromeric distances. Many functional dicentrics persist for months after formation. Even those with distantly spaced centromeres remain functionally dicentric for 20 cell generations. Other dicentrics within the population reflect centromere inactivation. In some cases, centromere inactivation occurs by an apparently epigenetic mechanism. In other dicentrics, the size of the alpha-satellite DNA array associated with CENP-A is reduced compared to the same array before dicentric formation. Extra-chromosomal fragments that contained CENP-A often appear in the same cells as dicentrics. Some of these fragments are derived from the same alpha-satellite DNA array as inactivated centromeres. Our results indicate that dicentric human chromosomes undergo alternative fates after formation. Many retain two active centromeres and are stable through multiple cell divisions. Others undergo centromere inactivation. This event occurs within a broad temporal window and can involve deletion of chromatin that marks the locus as a site for CENP-A maintenance/replenishment.

Hybrid error correction and de novo assembly of single-molecule sequencing reads.

Single-molecule sequencing instruments can generate multikilobase sequences with the potential to greatly improve genome and transcriptome assembly. However, the error rates of single-molecule reads are high, which has limited their use thus far to resequencing bacteria. To address this limitation, we introduce a correction algorithm and assembly strategy that uses short, high-fidelity sequences to correct the error in single-molecule sequences. We demonstrate the utility of this approach on reads generated by a PacBio RS instrument from phage, prokaryotic and eukaryotic whole genomes, including the previously unsequenced genome of the parrot Melopsittacus undulatus, as well as for RNA-Seq reads of the corn (Zea mays) transcriptome. Our long-read correction achieves >99.9% base-call accuracy, leading to substantially better assemblies than current sequencing strategies: in the best example, the median contig size was quintupled relative to high-coverage, second-generation assemblies. Greater gains are predicted if read lengths continue to increase, including the prospect of single-contig bacterial chromosome assembly.

Patterns of de novo allo B cells and antibody formation in chronic cardiac allograft rejection after alemtuzumab treatment.

Even though the etiology of chronic rejection (CR) is multifactorial, donor specific antibody (DSA) is considered to have a causal effect on CR development. Currently the antibody-mediated mechanisms during CR are poorly understood due to lack of proper animal models and tools. In a clinical setting, we previously demonstrated that induction therapy by lymphocyte depletion, using alemtuzumab (anti-human CD52), is associated with an increased incidence of serum alloantibody, C4d deposition and antibody-mediated rejection in human patients. In this study, the effects of T cell depletion in the development of antibody-mediated rejection were examined using human CD52 transgenic (CD52Tg) mice treated with alemtuzumab. Fully mismatched cardiac allografts were transplanted into alemtuzumab treated CD52Tg mice and showed no acute rejection while untreated recipients acutely rejected their grafts. However, approximately half of long-term recipients showed increased degree of vasculopathy, fibrosis and perivascular C3d depositions at posttransplant day 100. The development of CR correlated with DSA and C3d deposition in the graft. Using novel tracking tools to monitor donor-specific B cells, alloreactive B cells were shown to increase in accordance with DSA detection. The current animal model could provide a means of testing strategies to understand mechanisms and developing therapeutic approaches to prevent chronic rejection.

Enhanced de novo alloantibody and antibody-mediated injury in rhesus macaques.

Chronic allograft rejection is a major impediment to long-term transplant success. Humoral immune responses to alloantigens are a growing clinical problem in transplantation, with mounting evidence associating alloantibodies with the development of chronic rejection. Nearly a third of transplant recipients develop de novo antibodies, for which no established therapies are effective at preventing or eliminating, highlighting the need for a nonhuman primate model of antibody-mediated rejection. In this report, we demonstrate that depletion using anti-CD3 immunotoxin (IT) combined with maintenance immunosuppression that included tacrolimus with or without alefacept reliably prolonged renal allograft survival in rhesus monkeys. In these animals, a preferential skewing toward CD4 repopulation and proliferation was observed, particularly with the addition of alefacept. Furthermore, alefacept-treated animals demonstrated increased alloantibody production (100%) and morphologic features of antibody-mediated injury. In vitro, alefacept was found to enhance CD4 effector memory T cell proliferation. In conclusion, alefacept administration after depletion and with tacrolimus promotes a CD4+memory T cell and alloantibody response, with morphologic changes reflecting antibody-mediated allograft injury. Early and consistent de novo alloantibody production with associated histological changes makes this nonhuman primate model an attractive candidate for evaluating targeted therapeutics.

De novo generation of a non-segmented negative strand RNA virus with a bicistronic gene

Reverse genetics has facilitated the use of non-segmented negative strand RNA viruses (NNSV) as vectors. Currently, heterologous gene expression necessitates insertion of extra-numeral transcription units (ENTUs), which may alter the NNSV polar transcription gradient and attenuate growth relative to wildtype (Wt). We hypothesized that rescuing recombinant Sendai Virus (rSeV) with a bicistronic gene might circumvent this attenuation but still allow heterologous open reading frame (ORF) expression. Therefore, we used a 9-nucleotide sequence previously described with internal ribosome entry site (IRES) activity, which, when constructed as several repeats, synergistically increased the level of expression of the second cistron [Chappell, S.A., Edelman, G.M., Mauro, V.P., 2000. A 9-nt segment of a cellular mRNA can function as an internal ribosome entry site (IRES) and when present in linked multiple copies greatly enhances IRES activity. Proc. Natl. Acad. Sci. U.S.A. 97, 1536-1541]. We inserted the Renilla luciferase (rLuc) ORF, preceded by 1, 3 or 7 IRES copies, downstream of the SeV N ORF in an infectious clone. Corresponding rSeVs were successfully rescued. Interestingly, bicistronic rSeVs grew as fast as or faster than Wt rSeV. Furthermore, SeV gene transcription downstream of the N/rLuc gene was either equivalent to, or slightly enhanced, compared to Wt rSeV. Importantly, all rSeV/rLuc viruses efficiently expressed rLuc. IRES repetition increased rLuc expression at a multiplicity of infection of 0.1, although without evidence of synergistic enhancement. In conclusion, our approach provides a novel way of insertion and expression of foreign genes in NNSVs. (C) 2008 Elsevier B.V. All rights reserved.