192 resultados para Cotejo MPB


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The aim of this text is to provide a brief discussion on possible similarities, equal formulations and differences concerning internal level and international level from a critical comparison between a brief presentation of Antonio Gramsci’s and Kenneth Waltz theoretical formulations.

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This monograph addresses to the study of Latin metrical notions and concepts as showed in the volume VI of Heinrich Keil’s work, entitled Grammatici Latini (GL): Scriptores Artis Metricae. From that book, it was chosen Terentianus Maurus’s work for corpus, particularly the excerpt between verses 997 and 1299 of De Syllabis, a part of Terentianus’s De Litteris, De Syllabis, De Metris, which is a metric manual written in verses. Until now, there are only three translations of that text into modern languages: two in Italian (one of them was used for comparison with the translation prepared for this monograph) and a French one. That work still contains many reference notes whenever Terentianus Maurus uses ancient authors to demonstrate his explanations on Metrics. Thus this research intends to join in other analysis made on Latin metrical manuals that have already been translated or are still going to be, as well as to notice the importance and the specific value of meter in ancient poetry

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Pós-graduação em Agronomia (Produção Vegetal) - FCAV

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Pós-graduação em Letras - FCLAS

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Within a broader study of the apparatus governing the establishment of word classes in grammatical tradition, this study is centered on examining the treatment of the (sub)class of pronouns in two grammar guide groups from different periods. The collation was especially oriented by the existing general notion of a very strong link of the first grammarians with logic and, as a counterpart, of an insertion of the most recent works in the universe of a linguistic science and, at the same time, in the universe of a parameterization limiting actions and decisions.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Artes - IA

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This article makes a connection between Benjamin and Carpeaux in order to identify points of contact between the two authors. In this methodological operation assumes the crucial role collating Wege nach Rom with Benjamin's, Passages, which leads us to speak of a Carpeaux reader of Benjamin.

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Roberto Carlos é, ao mesmo tempo, o mais popular e o mais rejeitado dos artistas brasileiros. Nosso artigo pretende compreender as raízes dessa contradição, compreendendo tanto a positividade e o valor de sua obra - buscando relativizar uma série de estigmas e preconceitos que rondam sua figura, demonstrando, por assim dizer, a legitimidade de sua coroa - quanto o lugar a partir de onde falam seus antagonistas, de modo a perceber o conjunto de interesses e posições envolvidas nesses juízos críticos.

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Este artigo tem por objetivo analisar o conto "Ernesto de tal", de Machado de Assis, sob a perspectiva histórica e social. Será objeto de estudo também a comparação entre as duas versões do conto; a primeira, publicada no Jornal das Famílias, e a segunda, publicada no livro Histórias da meia-noite. Por meio da apreciação do cotejo entre as duas versões do conto em questão, procuraremos ainda refletir sobre como Machado criava e reescrevia seus textos.

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Agrobacterium VirB2 pilin is required for assembly of the VirB/VirD4 type IV secretion system (T4SS). The propilin is processed by signal sequence cleavage and covalent linkage of the N and C termini, and the cyclized pilin integrates into the inner membrane (IM) as a pool for assembly of the secretion channel and T pilus. Here, by use of the substituted cysteine accessibility method (SCAM), we defined the VirB2 IM topology and then identified distinct contributions of the T4SS ATPase subunits to the pilin structural organization. Labeling patterns of Cys-substituted pilins exposed to the membrane-impermeative, thiol-reactive reagent 3-(N-maleimidopropionyl)biocytin (MPB) supported a topology model in which two hydrophobic stretches comprise transmembrane domains, an intervening hydrophilic loop (residues 90 to 94) is cytoplasmic, and the hydrophilic N and C termini joined at residues 48 and 121 form a periplasmic loop. Interestingly, the VirB4 ATPase, but not a Walker A nucleoside triphosphate (NTP) binding motif mutant, induced (i) MPB labeling of Cys94, a residue that in the absence of the ATPase is located in the cytoplasmic loop, and (ii) release of pilin from the IM upon osmotic shock. These findings, coupled with evidence for VirB2-VirB4 complex formation by coimmunoprecipitation, support a model in which VirB4 functions as a dislocation motor to extract pilins from the IM during T4SS biogenesis. The VirB11 ATPase functioned together with VirB4 to induce a structural change in the pilin that was detectable by MPB labeling, suggestive of a role for VirB11 as a modulator of VirB4 dislocase activity.

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The goal of this study was to assess the in vitro differentiation capacity of human bone marrow-derived stem cells (hBMSCs) along retinal lineages. Mononuclear cells (MNC) were isolated from bone marrow (BM) and mobilized peripheral blood (mPB) using Ficoll-Paque density gradient centrifugation, and were sorted by magnetic-activated cell sorting (MACS) for specific stem cell subsets (CD34(+)CD38(+)/CD34(+)CD38(-)). These cells were then co-cultured on human retinal pigment epithelial cells (hRPE) for 7 days. The expression of stem cell, neural and retina-specific markers was examined by immunostaining, and the gene expression profiles were assessed after FACS separation of the co-cultured hBMSCs by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Furthermore, in vitro functionality of the differentiated cells was analyzed by quantifying phagocytosis of CY5-labeled photoreceptor outer segments (POS). After 7 days of co-culture, hBMSCs adopted an elongated epithelial-like morphology and expressed RPE-specific markers, such as RPE65 and bestrophin. In addition, these differentiated cells were able to phagocytose OS, one of the main characteristics of native RPE cells. Our data demonstrated that human CD34(+)CD38(-) hBMSC may differentiate towards an RPE-like cell type in vitro and could become a new type of autologous donor cell for regenerative therapy in retinal degenerative diseases.

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Actividades realizadas por el equipo de investigación durante el período 2011- 2012. 1. Revisión del Marco Teórico y cotejo de las categorías teóricas. 2. Trabajo de campo y reajuste de la información. 3. Selección y revisión bibliográfica 4. Lectura y análisis de la producción periodística en la provincia de Misiones y en las zonas fronterizas estudiadas. 5. Revisión de los problemas preliminares planteados para la investigación. 6. Elección y recorte temático sobre la vida cotidiana en el borde, puntos de vista que se relacionen con las experiencias significativas del lugar. 7. Realización de informes de avance de la investigación. 8. Realización del informe final de la investigación. Los ítems 7, 8, y 9 se transcriben en forma integral aunque respetando las tareas de cada investigador que lo hace desde sus aportes disciplinarios, y desde los diferentes sitios geográficos de la frontera de Misiones con Paraguay y Brasil.