Fungal biodeterioration of colour cinematographic films of the cultural heritage of Cuba

Until recently, cinematographic film was largely cellulose-triacetate-based. However, this material is highly susceptible to biodeterioration, thus placing historic film collections, an important part of the cultural heritage of many countries, at risk. In the present study, samples taken from several biodeteriorated color cinematographic films belonging to the collection of the Cuban Institute for Cinematographic Industry and Arts (ICAIC) were investigated. Infrared spectroscopy showed that all films were of the same composition, i.e., a gelatin emulsion coating one side of a cellulose-triacetate-based film support. The films were analyzed by environmental scanning electron microscopy and scanning electron microscopy to determine the degree of biodeterioration and the type of colonizing microorganisms. Significant fungal colonization was found on both sides of the films in all samples, with a higher concentration of fungi on the gelatin emulsion side. Epifluorescence microscopy of fluorochrome-dyed films demonstrated that some of the fungi were still active, indicating that the films under study, and probably others at the ICAIC, are at risk of further deterioration. Fungi were identified by molecular biology techniques. The fungi mainly responsible for the observed biodeterioration were those belonging to the genera Aspergillus and Cladosporium, although other genera, such as Microascus and Penicillium, were identified as well. In accordance with the findings described herein, the existing guidelines for the prevention and control of film biodeterioration are discussed.

Avaliação da integridade estrutural de elementos de concreto armado a partir das propriedades modais obtidas por técnicas de excitações aleatórias e transientes

A análise dinâmica experimental tem sido amplamente pesquisada como uma ferramenta de avaliação de integridade de estruturas de concreto armado. Existem técnicas de identificação de danos baseadas em propriedades modais como frequências de ressonâncias, deformadas modais, curvaturas modais e amortecimento. Há também técnicas baseadas na não linearidade da resposta dinâmica, que apesar do grande potencial na detecção de danos, têm sido pouco exploradas nos últimos anos. Este trabalho tem por objetivo avaliar a integridade estrutural de vigas de concreto armado através do comportamento da resposta dinâmica. Foram realizados ensaios dinâmicos em duas vigas de concreto armado com 3,5 m de comprimento, 25 cm de largura, 35 cm de altura e idênticas taxas de armaduras, mas configuradas com barras de aço de diferentes diâmetros, 2 ϕ 16 mm e 8 ϕ 8 mm, respectivamente. Tais vigas, inicialmente íntegras, foram submetidas a ciclos de carregamento e descarregamento com intensidades crescentes até atingir a ruptura do elemento. Após cada ciclo, as propriedades dinâmicas foram avaliadas experimentalmente, com o emprego de técnicas de excitação por sinais do tipo aleatório e tipo transiente, respectivamente, visando determinar parâmetros que indiquem a deterioração gradativa do elemento. Nesses ensaios dinâmicos aplicaram-se diferentes amplitudes da força de excitação. Verificou-se que o aumento da amplitude da força dinâmica de excitação provocou reduções nos valores das frequências de ressonância de 1,1% e 2,4%, associadas, respectivamente, às excitações aleatórias e transientes; e um comportamento não linear dos índices de amortecimento, associados às excitações aleatórias, mantendo um crescimento linear com as excitações transientes. Constatou-se, ainda, que os valores das frequências de ressonância decrescem com a redução de rigidez mecânica, diminuída com o aumento do nível de fissuração induzido nos modelos. Já os valores dos índices de amortecimento, após cada ciclo, se comportaram de forma não linear e assumiram diferentes valores, conforme a técnica de excitação empregada. Acredita-se que esta não linearidade está relacionada aos danos provocados no elemento pela solicitação estrutural e, por consequência, ao processo de como a dissipação de energia é empregada no processo de instauração, configuração e propagação das fissuras nos elementos de concreto armado.

L'Encarnella: revista satírica. Colección completa de seis números (1983-1984)

"L'Encarnella", revista satírica. Coleccion completa de seis numeros. 1983-1984: Número 1: “L’Encarnella entrega. Fulla oligofrènica per als bufats”, núm. 1.001, abril 1983. Tinta de color verde-gris oscuro. 4 p. + un encarte impreso a doble cara de 22 x 16 mm. Dep. Leg. A-229-1983. Número 2: “L’Encarnella encarnà. La revista més oberta per al lector més estret”, núm. 2.001, junio 1983. Tinta de color rojo vivo. 4 p. + un encarte impreso a una cara de 420 x 295 mm. Dep. Leg. A-514-1983. Número 3. “L’Encarnella festera. La fulla de got i punyal”, núm. 3.001, agosto 1983. Tinta de color morado oscuro. 4 p. Dep. Leg. A-714-1983. Número 4: “El rabo de l’encarnella. La fulla més verda per al lector més morat”, núm. 4.004, octubre 1983. Tinta de color verde brillante. 4 p. Dep. Leg. A-843-1983. Número 5: “Encarnelles blanques. Extra de navidad… con cotillón sorpresa. La revista que no produeix ressaca”, núm. 5.005, diciembre 1983. Tinta de color azul-negro oscuro. 8 p. + un encarte efímero consistente en un puñado de confetis sueltos de colores que le caían encima al lector cuando abría el ejemplar. Dep. Leg. A-1097-1983. Número 6: “L’Encarnella rostía. La fulla que no falla”, núm. 6.006, marzo 1983. Tinta de color azul oscuro. 4 p. Dep. Leg. A-164-1984.

Growth rates of Fe-Mn nodules and age and sedimentation rates of host sediments from the Southeast Pacific

Growth rates of nine ferromanganese nodules collected from the Southeast Pacific were estimated using the alpha radiogpaphic technigue. Growth rates range from 1 to 16 mm per million years. In three nodules measurements were made on two opposite sides; two of them showed no growth in one of measured directions during the last 300 ky, whereas in the third nodule growth rates on the opposite sides differ by factor 2. Average sedimentation rate of deposits underlying the nodules estimated by the radiocarbon and excess 230Th methods, were 4 mm/1000 years with rather minor variations. Difference between sedimentation rates and nodule growth rates is caused by activity of benthic fauna, as suggested by inversion of radiocarbon dates with depth.

Ice rafted debris distribution in sediment core CRP-1

Lonestone abundances in CRP-1 were investigated using three methods: core examination at Cape Roberts Camp, analysis of digital core images and follow-up core examination. For all images of split-core, we determined size and depth of every detectable lonestone larger than 3 mm. Lonestone abundance decreases exponentially with clast size. Although no significant depth-dependent variations in lonestone size distribution were detected, a strong 0.5-0.7 m abundance periodicity, of unknown origin, is evident within diamicts. Lonestone volume percentage was estimated from size distribution: most size classes contribute approximately the same volume to the total. Sizes >16 mm have rare enough lonestones that their counts are nonrepresentative when based on short intervals of split core. This problem does not affect total counts significantly, but the volume analysis needs to be confined to <= 6 mm lonestones to avoid instability induced by rare and nonrepresentative larger lonestones. If lonestone abundance can be used as an indicator of glacial proximity, then our CRP-1 lonestone abundance logs confirm the overall character of previously inferred variations in relative distance to the ice margin. Large-scale changes in lonestone abundance also reflect the CRP-1 sequence stratigraphy, with individual sequences generally characterised by basal lonestone-rich diamict overlain by lonestone-poor sands and muds. The relationship between glacial proximity and lonestone abundance within diamicts and within sand-mud intervals is, however, less certain. For example, two or three gradual lonestone increases may indicate regressions during glacial advances, in contrast to the more common CRP-l pattern of dominantly transgressive sequences.

Chemical abstracts.

"A publication of the Chemical Abstracts Service."

Growth, viral production and metabolism of a Helicoverpa zea cell line in serum-free culture

Insect cell cultures have been extensively utilised for means of production for heterologous proteins and biopesticides. Spodoptera frugiperda (Sf9) and Trichoplusia ni (High Five(TM)) cell lines have been widely used for the production of recombinant proteins, thus metabolism of these cell lines have been investigated thoroughly over recent years. The Helicoverpa zea cell line has potential use for the production of a biopesticide, specifically the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HaSNPV). The growth, virus production, nutrient consumption and waste production of this cell line was investigated under serum-free culture conditions, using SF900II and a low cost medium prototype (LCM). The cell growth ( growth rates and population doubling time) was comparable in SF900II and LCM, however, lower biomass and cell specific virus yields were obtained in LCM. H. zea cells showed a preference for asparagine over glutamine, similar to the High Five(TM) cells. Ammonia was accumulated to significantly high levels (16 mM) in SF900II, which is an asparagine and glutamine rich medium. However, given the absence of asparagine and glutamine in the medium ( LCM), H. zea cells adapted and grew well in the absence of these substrates and no accumulation of ammonia was observed. The adverse effect of ammonia on H. zea cells is unknown since good production of biologically active HaSNPV was achieved in the presence of high ammonia levels. H. zea cells showed a preference for maltose even given an abundance supply of free glucose. Accumulation of lactate was observed in H. zea cell cultures.

NARRATIVAS AUDIOVISUAIS NO PIAUI: Cultura e Sociedade na Linguagem do Cinema Universidade Metodista de São Paulo Programa de Pós-Graduação em Comunicação Social São Bernardo

O trabalho propõe uma investigação sobre a produção audiovisual piauiense, buscando entender como são negociadas as questões culturais e as especificidades das narrativas apresentadas no desenvolvimento histórico da cultura visual piauiense. Analisa-se, assim, as questões discursivas, imagéticas e tecnológicas que abordam aquela realidade cultural influenciada pelas vanguardas cinematográficas brasileiras, no caso o cinema novo e o cinema marginal. A pesquisa tem como corpus as produções superoitistas feitas a partir de 1972 até meados 1985, quando se encerra um segundo ciclo cinematográfico. Este estudo, de natureza qualitativa, emprega pesquisa bibliográfica e documental, com apoio em entrevistas e análise de documentos da época para o estudo das narrativas apresentadas pelas produções audiovisuais. Leva-se em consideração a influência das questões social, política, tecnológica e econômica do Piauí na construção desses filmes. Conclui-se que as práticas culturais e os recursos tecnológicos constituem uma cultura visual que representa as angústias e críticas locais e traduzem a tipificação do sujeito no Piauí.

Bile-salt-induced aggregation of poly(N-isopropylacrylamide) and lowering of the lower critical solution temperature in aqueous solutions

The effect of sodium cholate (NaC; concentration 1-16 mM), a biological surfactant, on the aggregation behavior of 1% (w/v, 2.2 × 10(-3) M) poly(N-isopropylacrylamide) (PNIPAM) aqueous solutions was studied as a function of temperature. From turbidity, dynamic light scattering, viscosity, and fluorescence measurements, it was observed that (i) there is NaC-induced nanoscale aggregation of PNIPAM in its sol state and (ii) the lower critical solution temperature corresponding to sol-gel transition shifts to a lower temperature by about 2 °C.

Growth curve of the lichen Rhizocarpon geographicum

Data on the growth curve of the lichen Rhizocarpon geographicum were obtained by measuring the radial growth rates (mm per 1.5 years) of 39 thalli from 2 to 65 mm in diameter growing in the same environment. An Aplin and Hill plot (r2 – r1 against ln r2 – ln r1) of the data and regression analyses suggested an initial phase of growth (up to a diameter of about 7 mm) in which the relative growth rate increased rapidly. This was followed by a phase in which the relative growth rate fell but the radial growth rate continued to rise (7 to 20 mm in diameter). Radial growth was then relatively constant until about 45 mm diameter and then declined. The Aplin and Hill model did not fit the data as a whole but may apply for a transient period in thalli between about 7 and 16 mm in diameter. The curve shows some similarities to that suggested by lichenometric studies but differs in showing a less steep decline in growth rate after the ‘great’ period.