Rational engineering of recombinant picornavirus capsids to produce safe, protective vaccine antigen

Foot-and-mouth disease remains a major plague of livestock and outbreaks are often economically catastrophic. Current inactivated virus vaccines require expensive high containment facilities for their production and maintenance of a cold-chain for their activity. We have addressed both of these major drawbacks. Firstly we have developed methods to efficiently express recombinant empty capsids. Expression constructs aimed at lowering the levels and activity of the viral protease required for the cleavage of the capsid protein precursor were used; this enabled the synthesis of empty A-serotype capsids in eukaryotic cells at levels potentially attractive to industry using both vaccinia virus and baculovirus driven expression. Secondly we have enhanced capsid stability by incorporating a rationally designed mutation, and shown by X-ray crystallography that stabilised and wild-type empty capsids have essentially the same structure as intact virus. Cattle vaccinated with recombinant capsids showed sustained virus neutralisation titres and protection from challenge 34 weeks after immunization. This approach to vaccine antigen production has several potential advantages over current technologies by reducing production costs, eliminating the risk of infectivity and enhancing the temperature stability of the product. Similar strategies that will optimize host cell viability during expression of a foreign toxic gene and/or improve capsid stability could allow the production of safe vaccines for other pathogenic picornaviruses of humans and animals.

Is there a gold social seal? The financial effects of additions to and deletions from social stock indices

This study investigates the financial effects of additions to and deletions from the most well-known social stock index: the MSCI KLD 400. Our study makes use of the unique setting that index reconstitution provides and allows us to bypass possible issues of endogeneity that commonly plague empirical studies of the link between corporate social and financial performance. By examining not only short-term returns but also trading activity, earnings per share, and long-term performance of stocks that are involved in these events, we bring forward evidence of a ‘social index effect’ where unethical transgressions are penalized more heavily than responsibility is rewarded. We find that the addition of a stock to the index does not lead to material changes in its market price, whereas deletions are accompanied by negative cumulative abnormal returns. Trading volumes for deleted stocks are significantly increased on the event date, while the operational performances of the respective firms deteriorate after their deletion from the social index.

Work and the adolescent in medieval England (AD 900-1550). The osteological evidence

What was it like to be a teenager in medieval England? Despite the fact that medieval society often singled young apprentices and workers out for comment, their study has been largely neglected in medieval archaeology. The skeletal remains of 4940 adolescents (6.6-25 years) from 151 sites in medieval England was compiled from a combination of primary data collection and secondary data from published and unpublished skeletal reports and on-line databases. The aim was to explore whether apprentices could be identified in the archaeological record and if so, at what age they started work and what impact occupation had on their health. The data were divided into urban and rural groups, dating from before and after the Black Death of AD 1348-9, and before the Industrial Revolution. A shift in the demographic pattern of urban and rural adolescents was identified after the Black Death, with a greater number of young females residing in the urban contexts after 14 years. The average age of males increased from 12 years to 14 years after the plague years, contrary to what we might expect from the documentary sources. There were higher rates of spinal and joint disease in the urban adolescents and their injuries were more widespread than their rural counterparts. Domestic service was the potential cause of the greater strain on the knees and backs of the urban females, with interpersonal violence evident in the young urban males. Overall, it was the urban females that carried the burden of respiratory and infectious diseases suggesting they may have been the most vulnerable group. This study has demonstrated the value of adolescent skeletal remains in revealing information about their health and working life, before and after the Black Death.

As comissões de conciliação prévia na justiça do trabalho: uma abordagem sobre sua importância e o esvaziamento ao longo da última década de sua vigência

O presente trabalho enfoca o que originou as Comissões de Conciliação Prévia, considerando os fatos relevantes que ensejaram sua criação, cujo embrião se formou no seio da maioria dos Ministros do Tribunal Superior do Trabalho, a partir de debates que culminaram com o patrocínio do projeto de lei que se materializou em janeiro de 2000. Realça a necessidade de um mecanismo de composição que não dependa do Judiciário, em decorrência não só do colapso em que se encontra a Justiça do Trabalho em razão do número de processos trabalhistas, como também na utilização de importante instrumento alternativo. Aborda também as diversas formas alternativas de solução de conflitos. Considera a presença do Conselho Nacional de Justiça que vem exigindo melhora na prestação jurisdicional. Demonstra que ao longo dos primeiros 10 anos da Lei que introduziu as CCPs, houve resistência de grande parte do Judiciário, o que acabou por esvaziá-las. Examina, em continuidade, as decisões proferidas ao longo da vigência da Lei e que influíram na atuação das Comissões de Conciliação Prévia. Finalmente, aponta os aspectos da Lei n.º 9.958/00, analisa a constitucionalidade e a natureza da mesma e demonstra a indispensabilidade da criação desse meio como forma de agilizar o Judiciário, de reduzir as demandas e, consequentemente, de auxiliar na efetividade da prestação da tutela jurisdicional. Observa, por fim, que o Judiciário não pode prescindir da colaboração de órgãos que possam auxiliar a minimizar o exagerado número de demandas que assolam aquele Poder.

Programa de Promoção da Igualdade de Oportunidade para Todos: experiências de ação afirmativa do Ministério Público do Trabalho (2003-2012)

Nos últimos anos o Brasil tem avançado no sentido de enfrentar as desigualdades raciais. Em 1995, o então Presidente da República Fernando Henrique Cardoso reconheceu oficial e publicamente não apenas a existência da discriminação racial e do preconceito de cor no País, mas também a necessidade de se adotar medidas públicas e privadas para fazer face ao problema. Naquele mesmo ano foi criado o Grupo de Trabalho Interministerial, que teve por incumbência propor ações integradas de combate à discriminação racial e recomendar políticas para a consolidação da cidadania da população negra. Para encaminhar as ações relacionadas ao mercado de trabalho, foi criado em 1996, no âmbito do Ministério do Trabalho, o Grupo de Trabalho para a Eliminação da Discriminação no Emprego e na Ocupação, cuja missão foi definir programas de ações que visassem ao combate da discriminação no emprego e na ocupação. Desde então, temos presenciado uma maior permeabilidade do Estado às demandas e proposições dos movimentos sociais negros. Marcos nesta trajetória foram a criação da SEPPIR – Secretaria de Promoção da Igualdade racial, em 2003, a aprovação do Estatuto da Igualdade Racial, em 2010, e a aprovação, por unanimidade, do sistema de cotas para negros no ensino superior brasileiro, pelo Superior Tribunal Federal, em 2011. Essas, dentre outras, são conquistas inquestionáveis e que revelam que o País vive um período de inflexão no encaminhamento de soluções para os problemas raciais que o assolam. Mas, se por um lado essas conquistas representam grandes vitórias, especialmente quando analisadas sob uma perspectiva retroativa, visto que desde a abolição da escravatura nenhuma ação estatal havia sido direcionada à solução de desigualdades raciais, por outro, muito ainda temos que avançar. Um olhar prospectivo revelará que o fosso criado ao longo desses séculos não poderia ser superado em apenas 20 anos. Muita desigualdade racial ainda resta. E muito ainda resta a ser feito para o seu enfrentamento. Ao “mobilizar conhecimento para a resolução de problemas sociais relevantes” (FARAH, 2012) esta tese busca contribuir para o avanço das políticas de igualdade racial no País, particularmente daquelas voltadas para o mercado de trabalho. Ela se constitui em um estudo de caso do Programa de Promoção da Igualdade de Oportunidade para Todos, uma iniciativa do Ministério Público do Trabalho, que teve por objetivo levar as organizações privadas a adotar ações afirmativas para o enfrentamento das desigualdades de gênero e raça nas relações de trabalho. Para a condução do estudo de caso único, empreendemos uma pesquisa qualitativa, adotando diversas técnicas de pesquisa, como a observação participante, conversas, entrevistas semiestruturadas e análise de documentos e outras materialidades, tendo o pós-construcionismo como sua perspectiva epistemológica (SPINK, 2005). Analisamos o Programa da Promoção da Igualdade de Oportunidade para Todos a partir das seguintes variáveis: sua concepção, implantação e primeiros resultados. Essa análise conduziu-nos a uma revisão da literatura nacional e estrangeira sobre a gestão da diversidade, e da literatura sobre desigualdade categórica durável. Os resultados deste estudo sugerem que, em que pesem as resistências enfrentadas para a sua implantação, o Programa já produziu efeitos positivos, embora também apontem seus limites e ajustes necessários para futuras políticas que visem à redução de desigualdade racial no mercado de trabalho no País.

Resíduos e subprodutos agroindustriais e grãos como substratos para produção do fungo entomapatogênico Lecanicillium lecanii

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Da escala médica de saúde à experiência social do saudável :diálogo entre os espaços dos bares e das academias fisiculturistas na cidade do Natal-RN

Currently, we attend a reverence of concepts ahead as health, life, youth and body. In we widen amount the ideals concerned to the healthful life, to the quality of life, the longevity and joviality and the extremities of the body represented by the illness, for the virus infection, the physical deficiency and the aging. Of the historical shades of the plague, of the hunger and the war that gagged the defenseless individual and its body, in the current days we increase the search for a full and powerful life, independent of a religious imaginary to predict the epidemic curse, the threat represented for the sick people and the incarnate divine anger in the death; or of medical science presented in the spaces of the technology and the physiology, being left fragile the social and psychological dimensions of the human confined to the patient issue and, finally, the commanded urban health politics in quantitative goals of hygienic cleaning, of the medical techniques and the education citizen. For beyond these instances, emerges in our days a plural, close and biographical agreement well of the body and welfare. On the other hand, an understanding of the healthful life and well-being that more certifies the presence of something the one that clear landmarks amongst normality and the irregularity, the esteem and the destructive vice: it has a projection of healthful life measures without conceptual models of body and health under the doctor-scientific standard occidental. This thematic one will confide in way to the enclosure for spaces to the muscles and fitness exercises and the bars from the city of Natal, in the state of Rio Grande do Norte, Brazil, while comprehensive interchange concerning as the individual comes dealing with the notion the body and health and, mainly, if perceiving inside of its body and its health. Amongst these two spaces of typical leisure of the modern urban phenomenon, the hedonism bodily with its muscles and salience and one another form of hedonism in the fruition of allowed drugs will be across itself in a dialogue about which social s relationships are really in game in an imaginary construction amongst an doctor-aesthetic ideal of health and the social and subjective experience in the option for a healthful life

Maracatus Solar e Reis de Paus: tradição e modernidade no carnaval de rua em Fortaleza

This is an ethnographic and comparative study of the Maracatus Solar (2006) and Reis de Paus (1960), whose aim was to verify what is ancient and traditional in the new maracatu practiced by the guild Solar and conversely, what is new or modern in the old maracatu ritualized by guild Reis de Paus. It is worth noting that through this case study it is also intended to ethnographically observe and better understand the processes of ruptures and continuities between modernization and tradition, and the relationship between the global and the local. The communication system, the dancing, the music, the costumes and the loas (letters) were analyzed using the technique of participant observation as well as secondary materials such as newspapers, blogs and magazines. The interviews were open, non-directive, but recorded to facilitate understanding the speech of revelers. The research has shown that all the symbolic elements of aesthetic expression of the maracatu are permeated by clashes of historical contexts and of political representation, which, in another instance, also enunciates a fight of micro-community resistance regarding the renewal process and the social development that plague modern megalopolis. It is In this interim, between modernity and tradition that today it can be spoken about the existence of hybrid identity in the maracatu regarding a context mediated by the overall above mentioned values and customs specific of the new generations. However, one can not deny that the forms of negotiations with modernity also require the establishment of a link with the specific singularity of a popular culture that is not excluded, but also should not get invaded by the idea of authenticity. Therefore, performing this study was above all an opportunity to understand also the community life in the city outskirts, understanding society, culture and everyday social relations maintained between humans that produce and make it all happen. The Solar and Reis de Paus do not join in opposition between themselves nor by their similarity. What is most striking among them is the renewal of a tradition that reinvents itself in the form of popular representation across the street parade

Diamondback moth oviposition nonpreference in cauliflower genotypes

The Diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is one of the main plague-insect specie of Brassicaceae plants in Brazil and all over the world. The resistant genotypes use to its control is a promising alternative. This work aimed evaluates the eggs distribution along the plant, the adults' density per plant, and determine the cauliflower genotypes effect in the P. xylostella oviposition. The experiment was carried out at FCAV/UNESP-Jaboticabal Campus Phytossanity Department (Departamento de Fitossanidade). It was evaluated the eggs distribution, the P. xylostella adults density effect using Sharon hybrid, and tests with or without choose choice to determine the P. xylostella nonpreference in the Teresopolis Gigante, Verona, Barcelona, Sharon, Silver Streak, and Piracicaba Precoce genotypes. It is possible conclude that P. xylostella has higher willingness to oviposits in the stem than in the basal leaves. The three couple density of P. xylostella per plant is the best to discriminate cauliflower genotypes regarding the resistance grade to nonpreference choose choice to oviposition. During the P. xylostella oviposition preference tests with choose choice, the genotypes Sharon, Piracicaba Precoce, Barcelona, Verona e Teresopolis Gigante are less desirable to oviposition; while during the no choose choice tests the genotypes did not differ among them.

Alphitobius diaperinus spp como veiculador de Clostridium perfringens em granjas avícolas do interior paulista - Brasil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Agronomic characteristics of 'Prata-anã' and 'Maçã' bananas micropropagated

O experimento foi instalado com o objetivo de estudar o desempenho agronômico de cultivares de bananeira Prata-anã e Maçã, na região de São Manuel-SP. Foram avaliadas as seguintes características de desenvolvimento, no primeiro ciclo de produção: número de dias do plantio à floração e da mesma à colheita, número de rebentos (filhos) emitidos até o surgimento da inflorescência e na época da colheita, número de folhas ativas na época do aparecimento da inflorescência e da colheita, e características de rendimento, como peso médio do cacho, ráquis, penca e fruto, número médio de frutos por penca e cacho, comprimento e diâmetro médio dos frutos. Também foi avaliada a incidência de pragas e doenças. Foi empregado o delineamento inteiramente casualizado, com 2 tratamentos, 15 repetições e 5 plantas úteis por parcela experimental. As duas cultivares foram caracterizadas pelos valores das estatísticas descritivas, média e desvio-padrão, para as características de interesse no experimento. Foram estabelecidas possíveis correlações entre medidas de desenvolvimento e produção. As duas cultivares apresentaram ciclo do plantio até a colheita de 574 dias para 'Prata-anã' e 567 dias para 'Maçã', sendo que o ciclo produtivo foi maior na 'Prata-anã'. Não houve problemas de pragas e doenças em todo o ciclo de produção, nas duas cultivares avaliadas.

Brazilian spotted fever: A reemergent zoonosis

Brazilian spotted fever is caused by the bacterium Rickettsia rickettsii, which is the most pathogenic species of the spotted-fever rickettsiae group and is transmitted by the bite of infected ticks. Amblyomma cajennense is the most important tick species involved in the cycle of this zoonosis in Brazil as it presents low host specificity, great number of natural reservoirs and wide geographic distribution. It was first described in the state of São Paulo in 1929 and later in Rio de Janeiro, Minas Gerais and Bahia. The number of cases decreased in the 1940's with the development of new plague control techniques and antibiotics. In the last decades, the number of new cases has increased. The current review aimed at reporting some of the epidemiological and public health aspects of this reemergent disease with new foci, mainly in the southeastern region of Brazil.

Fipronil-induced cell death in salivary glands of Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) semi-engorged females

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Noise and ultraviolet divergences in the dynamics of the chiral condensate in QCD

The time evolution of the matter produced in high energy heavy-ion collisions seems to be well described by relativistic viscous hydrodynamics. In addition to the hydrodynamic degrees of freedom related to energy-momentum conservation, degrees of freedom associated with order parameters of broken continuous symmetries must be considered because they are all coupled to each other. of particular interest is the coupling of degrees of freedom associated with the chiral symmetry of QCD. Quantum and thermal fluctuations of the chiral fields act as noise sources in the classical equations of motion, turning them into stochastic differential equations in the form of Ginzburg-Landau-Langevin (GLL) equations. Analytic solutions of GLL equations are attainable only in very special circumstances and extensive numerical simulations are necessary, usually by discretizing the equations on a spatial lattice. However, a not much appreciated issue in the numerical simulations of GLL equations is that ultraviolet divergences in the form of lattice-spacing dependence plague the solutions. The divergences are related to the well-known Rayleigh-Jeans catastrophe in classical field theory. In the present communication we present a systematic lattice renormalization method to control the catastrophe. We discuss the implementation of the method for a GLL equation derived in the context of a model for the QCD chiral phase transition and consider the nonequilibrium evolution of the chiral condensate during the hydrodynamic flow of the quark-gluon plasma.

The Combination of Amoxicillin and Metronidazole Improves Clinical and Microbiologic Results of One-Stage, Full-Mouth, Ultrasonic Debridement in Aggressive Periodontitis Treatment

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)