Overcoming diversity with a multi-envelope HIV vaccine

A central obstacle to the design of a global HIV vaccine is viral diversity. Antigenic differences in envelope proteins result in distinct HIV serotypes, operationally defined such that antibodies raised against envelope from one serotype will not bind envelope molecules from a different serotype. The existence of serotypes has presented a similar challenge to vaccine development against other pathogens. In such cases, antigenic diversity has been addressed by vaccine design: for example, the poliovirus vaccine includes 3 serotypes of poliovirus, and Pneumovax® presents a cocktail of 23 pneumococcal variants to the immune system. It is likely that a successful vaccine for HIV must also comprise a cocktail of antigens. Here, data relevant to the development of cocktail vaccines, designed to harness diverse, envelope-specific B-cell and T-cell responses, are reviewed.

Overcoming inconsistencies in placement assessment : the case for developmental assessment centers

Placements are integral to many university courses and to increasing student employability skills. Nevertheless, several complications, such as the assessment of placement experiences which often go against the principles of procedural justice, may limit placement effectiveness. For example, procedures are not applied uniformly across students; and evaluations of intangible qualities are susceptible to biases. As a result, effort and learning can be compromised. This paper advocates the use of developmental assessment centers to help solve these shortcomings. Developmental assessment centers are often used in organizations to evaluate capabilities of individuals and to facilitate development. Participants complete a series of work related and standardized tasks. Multiple raters then utilize a systematic approach to evaluate participants on a range of competencies, and consequently present constructive feedback to facilitate learning. Therefore, developmental assessment center principles match the key determinants of procedural justice and thus overcome many problems with traditional placement assessments.

Making a visible difference : overcoming barriers using arts-based learning

This paper reports on a small-scale action research project conducted by two university-based researchers using a visual arts method. The seven-week drawing programme was the second cycle of an action research project. The participants were nine boys of primary school age variously identified by their teachers as reluctant readers and/or as struggling with print literacy. The thematic concerns addressed in the paper fall into two broad categories: motivating learners by drawing on their popular cultural capital and interests, as well as tracing the construction of visual texts as data in action research. Findings are presented in response to the following research questions: What happens to those students who do not or cannot meet the required standards in literacy? What kind of research approach can be utilised or adapted to respond to the literacy practices and behaviours of young people?

Overcoming interfacial affinity issues in natural fiber reinforced polylactide biocomposites by surface adsorption of amphiphilic block copolymers

This work demonstrates that the interfacial properties in a natural fiber reinforced polylactide biocomposite can be tailored through surface adsorption of amphiphilic and biodegradable poly (ethylene glycol)-b-poly-(L-lactide) (PEG-PLLA) block copolymers. The deposition from solvent solution of PEG-PLLA copolymers onto the fibrous substrate induced distinct mechanisms of molecular organization at the cellulosic interface, which are correlated to the hydrophobic/hydrophilic ratios and the type of solvent used. The findings of the study evidenced that the performance of the corresponding biocomposites with polylactide were effectively enhanced by using these copolymers as interfacial coupling agents. During the fabrication stage, diffusion of the polylactide in the melt induced a change in the environment surrounding block copolymers which became hydrophobic. It is proposed that molecular reorganization of the block copolymers at the interface occurred, which favored the interactions with both the hydrophilic fibers and hydrophobic polylactide matrix. The strong interactions such as intra- and intermolecular hydrogen bonds formed across the fiber−matrix interface can be accounted for the enhancement in properties displayed by the biocomposites. Although the results reported here are confined, this concept is unique as it shows that by tuning the amphiphilicity and the type of building blocks, it is possible to control the surface properties of the substrate by self-assembly and disassembly of the amphiphiles for functional materials.

Overcoming data constraints to create meaningful ecological models

Many techniques used to model ecosystems cannot be meaningfully applied to large-scale ecological problems due to data constraints. Disparate collection methods, data types and incomplete data sets, or limited theoretical understanding mean that a wide range of modelling techniques used to model physical processes or for problems specific to species or populations cannot be used at an ecosystem scale. In developing an ecological response model for the Coorong, a South Australian hypersaline estuary, we combined several flexible modelling approaches in a statistical framework to develop an approach we call ‘ecosystem states’. This model uses simulated hydrodynamic conditions as input to predict one of a suite of states per space and time, allowing prediction of likely ecological conditions under a variety of scenarios. Each ecosystem state has defined sets of biota and physico-chemical parameters. The existing model is limited in that its predictions have yet to be tested and, as yet, no spatial or temporal connectivity has been incorporated into simulated time series of ecosystem states. This approach can be used in a wide range of ecosystems, where enough data are available to model ecosystem states. We are in the process of applying the technique to a nearby lake system. This has been more difficult than for the Coorong as there is little overlap in the spatial and temporal coverage of biological data sets for that region. The approach is robust to low-quality biological data and missing environmental data, so should suit situations where community or management monitoring programs have occurred through time.

Optimal effort investment for overcoming the weakest point: new insights from a computational model of neuromuscular adaptation

The occurrence of so-called sticking points in a lift is pervasive in weight training practice. Biomechanically complex exercises often exhibit multi modal variation of effective force exerted against the load as a function of the elevation and velocity of the load. This results in a variety of possible loci for the occurrence of sticking points and makes the problem of designing the optimal training strategy to overcome them challenging. In this article a case founded on theoretical grounds is made against a purely empirical method. It is argued that the nature of the problem considered and the wide range of variables involved limit the generality of conclusions which can be drawn from experimental studies alone. Instead an alternative is described, whereby a recently proposed mathematical model of neuromuscular adaptation is employed in a series of computer simulations. These are used to examine quantitatively the effects of differently targeted partial range of motion (ROM) training approaches. Counter-intuitively and in contrast to common training practices, the key novel insight inferred from the obtained results is that in some cases the most effective approach for improving performance in an exercise with a sticking point at a particular point in the ROM is to improve force production capability at a different and possibly remote position in the lift. In the context of the employed model, this result is explained by changes in the neuromuscular and biomechanical environment for force production.

Development by design in Western Australia : Overcoming offset obstacles

Biodiversity offsets can be an important tool for maintaining or enhancingenvironmental values in situations where development is sought despite negativeenvironmental impacts. There are now approximately 45 compensatory mitigationprograms for biodiversity impacts worldwide, with another 27 programs in development. While offsets have great potential as a conservation tool, their establishment requires overcoming a number of conceptual and methodological hurdles. In Australia, new policy changes at the national and state (i.e., Western Australia) level require that offsets follow a set of general principles: (1) Environmental offsets may not be appropriate for all projects and will only be considered after avoidance and mitigation options have been pursued;(2) Environmental offsets will be based on sound environmental information andknowledge; (3) Establishing goals for offsets requires an estimate of expected direct and indirect impacts; (4) Environmental offsets will be focused on longer term strategic outcomes; (5) Environmental offsets will be cost-effective, as well as relevant and proportionate to the significance of the environmental value being impacted. Here we focus on the challenges of determining and implementing offsets using a real world example from a voluntary offset process undertaken for Barrick Gold’s Kanowna Belle mine site in Western Australia to highlight those challenges and potential solutions.