Omega-3 fatty acids and the neurovascular responses to mental stress in humans

Hypertension is the most prevalent form of cardiovascular disease (CVD) in the world, and is known to increase the risk for developing other diseases. Recently, the American Heart Association introduced a new classification of blood pressure, prehypertension (PHT). The criteria for PHT include a systolic of 120-139 mmHg and/or a diastolic blood pressure of 80-89 mmHg. It has been observed that individuals with PHT have a higher risk of developing hypertension later in life. Therefore, it is important to understand the mechanisms contributing to PHT in order to possibly prevent hypertension. Omega-3 fatty acids found in fish oils have been suggested as a means of lowering blood pressure. However, little is known on the effects of fish oil in PHT humans. Therefore we conducted two studies. In Study 1 we investigated PHT and normotensive (NT) individuals during a mental stress task. Mental stress is known to contribute to the development of hypertension. In Study 2 PHT and NT subjects were placed in an eight week double-blind placebo controlled study in which subjects consumed 9g/day of either fish oil or placebo (olive oil) in addition to their regular diets. Subjects were tested during a resting baseline (seated and supine), 5 minutes of a mental stress task, and 5 minutes of recovery both pre and post supplementation. We measured arterial pressure (AP), heart rate (HR), muscle sympathetic nerve activity (MSNA), and forearm and calf vascular responses. In Study 1 PHT demonstrated augmented AP and blunted vasodilation during mental stress, but MSNA did not change. In Study 2, fish oil did not directly influence blood pressure, MSNA or vascular responses to mental stress. However, it became clear that fish oil had an effect on some but not all subjects (both PHT and NT). Specifically, subjects who experienced a reduced blood pressure response to fish oil also demonstrated a decrease in MSNA and HR during mental stress. Collectively, the investigations in this dissertation had several novel findings. First, PHT individuals demonstrate an augmented pressor and blunted vascular response to mental stress, a response that may be contributing to the development of hypertension. Second, fish oil does not consistently lower resting blood pressure, but the interindividual responses may be related to MSNA. Third, fish oil attenuated the heart rate and MSNA responses and to mental stress in both PHT and NT. In conclusion, we found that there are both similarities and differences in the way PHT and NT individuals respond to mental stress and fish oil.

SLC9/NHE gene family, a plasma membrane and organellar family of Na⁺/H⁺ exchangers

This brief review of the human Na/H exchanger gene family introduces a new classification with three subgroups to the SLC9 gene family. Progress in the structure and function of this gene family is reviewed with structure based on homology to the bacterial Na/H exchanger NhaA. Human diseases which result from genetic abnormalities of the SLC9 family are discussed although the exact role of these transporters in causing any disease is not established, other than poorly functioning NHE3 in congenital Na diarrhea.

Analysis of Phenotypic Variation in Childhood Wheezing Disorders

Recurrent wheezing or asthma is a common problem in children that has increased considerably in prevalence in the past few decades. The causes and underlying mechanisms are poorly understood and it is thought that a numb er of distinct diseases causing similar symptoms are involved. Due to the lack of a biologically founded classification system, children are classified according to their observed disease related features (symptoms, signs, measurements) into phenotypes. The objectives of this PhD project were a) to develop tools for analysing phenotypic variation of a disease, and b) to examine phenotypic variability of wheezing among children by applying these tools to existing epidemiological data. A combination of graphical methods (multivariate co rrespondence analysis) and statistical models (latent variables models) was used. In a first phase, a model for discrete variability (latent class model) was applied to data on symptoms and measurements from an epidemiological study to identify distinct phenotypes of wheezing. In a second phase, the modelling framework was expanded to include continuous variability (e.g. along a severity gradient) and combinations of discrete and continuo us variability (factor models and factor mixture models). The third phase focused on validating the methods using simulation studies. The main body of this thesis consists of 5 articles (3 published, 1 submitted and 1 to be submitted) including applications, methodological contributions and a review. The main findings and contributions were: 1) The application of a latent class model to epidemiological data (symptoms and physiological measurements) yielded plausible pheno types of wheezing with distinguishing characteristics that have previously been used as phenotype defining characteristics. 2) A method was proposed for including responses to conditional questions (e.g. questions on severity or triggers of wheezing are asked only to children with wheeze) in multivariate modelling.ii 3) A panel of clinicians was set up to agree on a plausible model for wheezing diseases. The model can be used to generate datasets for testing the modelling approach. 4) A critical review of methods for defining and validating phenotypes of wheeze in children was conducted. 5) The simulation studies showed that a parsimonious parameterisation of the models is required to identify the true underlying structure of the data. The developed approach can deal with some challenges of real-life cohort data such as variables of mixed mode (continuous and categorical), missing data and conditional questions. If carefully applied, the approach can be used to identify whether the underlying phenotypic variation is discrete (classes), continuous (factors) or a combination of these. These methods could help improve precision of research into causes and mechanisms and contribute to the development of a new classification of wheezing disorders in children and other diseases which are difficult to classify.

Towards mapping the late Quaternary vegetation change of Europe

The number of well-dated pollen diagrams in Europe has increased considerably over the last 30 years and many of them have been submitted to the European Pollen Database (EPD). This allows for the construction of increasingly precise maps of Holocene vegetation change across the continent. Chronological information in the EPD has been expressed in uncalibrated radiocarbon years, and most chronologies to date are based on this time scale. Here we present new chronologies for most of the datasets stored in the EPD based on calibrated radiocarbon years. Age information associated with pollen diagrams is often derived from the pollen stratigraphy itself or from other sedimentological information. We reviewed these chronological tie points and assigned uncertainties to them. The steps taken to generate the new chronologies are described and the rationale for a new classification system for age uncertainties is introduced. The resulting chronologies are fit for most continental-scale questions. They may not provide the best age model for particular sites, but may be viewed as general purpose chronologies. Taxonomic particularities of the data stored in the EPD are explained. An example is given of how the database can be queried to select samples with appropriate age control as well as the suitable taxonomic level to answer a specific research question.

Hysteroscopic outpatient metroplasty to expand dysmorphic uteri (HOME-DU technique): a pilot study.

The new classification system of uterine anomalies from the European Society of Human Reproduction and Embryology and the European Society for Gynaecological Endoscopy defines T-shaped and tubular-shaped infantilis uteri as 'dysmorphic'. Such malformations have been proven to be associated with poor reproductive performance. A prospective observational study was conducted with 30 infertile women with dysmorphic uterus who underwent the novel Hysteroscopic Outpatient Metroplasty to Expand Dysmorphic Uteri (HOME-DU ) technique. Incisions are made on the uterine walls with a 5 Fr bipolar electrode. The procedure was conducted in outpatients under conscious sedation, using a 5-mm office hysteroscope. The technique was successful in all cases without complications. A net increase of uterine volume was found, as measured at hysteroscopy and three-dimensional transvaginal ultrasound (P < 0.001). Uterine morphology improved in all patients but one. At mean follow-up of 15 months, clinical pregnancy rate was 57% and term delivery rate 65%. These early data support HOME-DU as safe and effective in expanding the volume and normalizing the appearance of the uterine cavity of dysmorphic uteri. Although the cohort was small, pregnancy and live births outcomes were favourable in this poor-prognosis group, implying desirable benefits, which should be compared with other techniques.

Validated sample ages from 823 EPD sites

The number of well-dated pollen diagrams in Europe has increased considerably over the last 30 years and many of them have been submitted to the European Pollen Database (EPD). This allows for the construction of increasingly precise maps of Holocene vegetation change across the continent. Chronological information in the EPD has been expressed in uncalibrated radiocarbon years, and most chronologies to date are based on this time scale. Here we present new chronologies for most of the datasets stored in the EPD based on calibrated radiocarbon years. Age information associated with pollen diagrams is often derived from the pollen stratigraphy itself or from other sedimentological information. We reviewed these chronological tie points and assigned uncertainties to them. The steps taken to generate the new chronologies are described and the rationale for a new classification system for age uncertainties is introduced. The resulting chronologies are fit for most continental-scale questions. They may not provide the best age model for particular sites, but may be viewed as general purpose chronologies. Taxonomic particularities of the data stored in the EPD are explained. An example is given of how the database can be queried to select samples with appropriate age control as well as the suitable taxonomic level to answer a specific research question.

(Table A1) Species list of the high Antarctic Weddell Sea food web

Human-induced habitat destruction, overexploitation, introduction of alien species and climate change are causing species to go extinct at unprecedented rates, from local to global scales. There are growing concerns that these kinds of disturbances alter important functions of ecosystems. Our current understanding is that key parameters of a community (e.g. its functional diversity, species composition, and presence/absence of vulnerable species) reflect an ecological network's ability to resist or rebound from change in response to pressures and disturbances, such as species loss. If the food web structure is relatively simple, we can analyse the roles of different species interactions in determining how environmental impacts translate into species loss. However, when ecosystems harbour species-rich communities, as is the case in most natural systems, then the complex network of ecological interactions makes it a far more challenging task to perceive how species' functional roles influence the consequences of species loss. One approach to deal with such complexity is to focus on the functional traits of species in order to identify their respective roles: for instance, large species seem to be more susceptible to extinction than smaller species. Here, we introduce and analyse the marine food web from the high Antarctic Weddell Sea Shelf to illustrate the role of species traits in relation to network robustness of this complex food web. Our approach was threefold: firstly, we applied a new classification system to all species, grouping them by traits other than body size; secondly, we tested the relationship between body size and food web parameters within and across these groups and finally, we calculated food web robustness. We addressed questions regarding (i) patterns of species functional/trophic roles, (ii) relationships between species functional roles and body size and (iii) the role of species body size in terms of network robustness. Our results show that when analyzing relationships between trophic structure, body size and network structure, the diversity of predatory species types needs to be considered in future studies.

Composition of hydrothermal manifestations in the World Ocean

The monograph summarizes results of studies of hydrothermal fields on the ocean floor, hydrothermal plumes and metalliferous sediments. Hydrothermal ore manifestations formed in different geodynamic settings, with different character of volcanism in different facial conditions of deposition are described. Causes of non-uniformity of hydrothermal system functioning in different parts of the ocean and therefore variability of hydrothermal deposits are under consideration. On the base of found relationships of these irregularities with geodynamics, volcanism and sedimentation a new classification of hydrothermal processes and genetic models of hydrothermal ore formation in the ocean have been created. Regularities of hydrothermal sedimentary material dispersion in bottom waters are discussed.

A survey of feature selection in Internet traffic characterization

In the last decade, the research community has focused on new classification methods that rely on statistical characteristics of Internet traffic, instead of pre-viously popular port-number-based or payload-based methods, which are under even bigger constrictions. Some research works based on statistical characteristics generated large fea-ture sets of Internet traffic; however, nowadays it?s impossible to handle hun-dreds of features in big data scenarios, only leading to unacceptable processing time and misleading classification results due to redundant and correlative data. As a consequence, a feature selection procedure is essential in the process of Internet traffic characterization. In this paper a survey of feature selection methods is presented: feature selection frameworks are introduced, and differ-ent categories of methods are briefly explained and compared; several proposals on feature selection in Internet traffic characterization are shown; finally, future application of feature selection to a concrete project is proposed.

Modelado y Comparación de Colecciones en la Web Semántica

En esta tesis se estudia la representación, modelado y comparación de colecciones mediante el uso de ontologías en el ámbito de la Web Semántica. Las colecciones, entendidas como agrupaciones de objetos o elementos con entidad propia, son construcciones que aparecen frecuentemente en prácticamente todos los dominios del mundo real, y por tanto, es imprescindible disponer de conceptualizaciones de estas estructuras abstractas y de representaciones de estas conceptualizaciones en los sistemas informáticos, que definan adecuadamente su semántica. Mientras que en muchos ámbitos de la Informática y la Inteligencia Artificial, como por ejemplo la programación, las bases de datos o la recuperación de información, las colecciones han sido ampliamente estudiadas y se han desarrollado representaciones que responden a multitud de conceptualizaciones, en el ámbito de la Web Semántica, sin embargo, su estudio ha sido bastante limitado. De hecho hasta la fecha existen pocas propuestas de representación de colecciones mediante ontologías, y las que hay sólo cubren algunos tipos de colecciones y presentan importantes limitaciones. Esto impide la representación adecuada de colecciones y dificulta otras tareas comunes como la comparación de colecciones, algo crítico en operaciones habituales como las búsquedas semánticas o el enlazado de datos en la Web Semántica. Para solventar este problema esta tesis hace una propuesta de modelización de colecciones basada en una nueva clasificación de colecciones de acuerdo a sus características estructurales (homogeneidad, unicidad, orden y cardinalidad). Esta clasificación permite definir una taxonomía con hasta 16 tipos de colecciones distintas. Entre otras ventajas, esta nueva clasificación permite aprovechar la semántica de las propiedades estructurales de cada tipo de colección para realizar comparaciones utilizando las funciones de similitud y disimilitud más apropiadas. De este modo, la tesis desarrolla además un nuevo catálogo de funciones de similitud para las distintas colecciones, donde se han recogido las funciones de (di)similitud más conocidas y también algunas nuevas. Esta propuesta se ha implementado mediante dos ontologías paralelas, la ontología E-Collections, que representa los distintos tipos de colecciones de la taxonomía y su axiomática, y la ontología SIMEON (Similarity Measures Ontology) que representa los tipos de funciones de (di)similitud para cada tipo de colección. Gracias a estas ontologías, para comparar dos colecciones, una vez representadas como instancias de la clase más apropiada de la ontología E-Collections, automáticamente se sabe qué funciones de (di)similitud de la ontología SIMEON pueden utilizarse para su comparación. Abstract This thesis studies the representation, modeling and comparison of collections in the Semantic Web using ontologies. Collections, understood as groups of objects or elements with their own identities, are constructions that appear frequently in almost all areas of the real world. Therefore, it is essential to have conceptualizations of these abstract structures and representations of these conceptualizations in computer systems, that define their semantic properly. While in many areas of Computer Science and Artificial Intelligence, such as Programming, Databases or Information Retrieval, the collections have been extensively studied and there are representations that match many conceptualizations, in the field Semantic Web, however, their study has been quite limited. In fact, there are few representations of collections using ontologies so far, and they only cover some types of collections and have important limitations. This hinders a proper representation of collections and other common tasks like comparing collections, something critical in usual operations such as semantic search or linking data on the Semantic Web. To solve this problem this thesis makes a proposal for modelling collections based on a new classification of collections according to their structural characteristics (homogeneity, uniqueness, order and cardinality). This classification allows to define a taxonomy with up to 16 different types of collections. Among other advantages, this new classification can leverage the semantics of the structural properties of each type of collection to make comparisons using the most appropriate (dis)similarity functions. Thus, the thesis also develops a new catalog of similarity functions for the different types of collections. This catalog contains the most common (dis)similarity functions as well as new ones. This proposal is implemented through two parallel ontologies, the E-Collections ontology that represents the different types of collections in the taxonomy and their axiomatic, and the SIMEON ontology (Similarity Measures Ontology) that represents the types of (dis)similarity functions for each type of collection. Thanks to these ontologies, to compare two collections, once represented as instances of the appropriate class of E-Collections ontology, we can know automatically which (dis)similarity functions of the SIMEON ontology are suitable for the comparison. Finally, the feasibility and usefulness of this modeling and comparison of collections proposal is proved in the field of oenology, applying both E-Collections and SIMEON ontologies to the representation and comparison of wines with the E-Baco ontology.

GÊNEROS RADIOJORNALÍSTICOS: Análise da Rádio Eldorado de São Paulo

A tese de doutorado insere-se no âmbito da Processos Comunicacionais, tendo como projeto temático a Midiologia Comparada. Tem como objetivo propor uma nova classificação para os gêneros radiojornalísticos, a partir do levantamento e da análise comparativa da bibliografia existente sobre o tema, confrontando-a posteriormente com a programação efetiva de três programas de uma das mais tradicionais emissoras segmentadas em jornalismo de São Paulo: a Rádio Eldorado AM. Quanto à metodologia, primeiramente efetuamos uma análise de conteúdo, a fim de compreender a utilização de tais formatos dentro de gêneros estabelecidos e, assim, propor uma nova classificação dos gêneros jornalísticos brasileiros no rádio, conforme o objetivo geral deste estudo. Num segundo momento realizamos o levantamento da ocorrência dos formatos jornalísticos nos três programas radiofônicos analisados. Ao final, concluímos que os gêneros radiojornalísticos não têm sido bem explorados pelos veículos ditos all news e talk and news.(AU)

GÊNEROS RADIOJORNALÍSTICOS: Análise da Rádio Eldorado de São Paulo

A tese de doutorado insere-se no âmbito da Processos Comunicacionais, tendo como projeto temático a Midiologia Comparada. Tem como objetivo propor uma nova classificação para os gêneros radiojornalísticos, a partir do levantamento e da análise comparativa da bibliografia existente sobre o tema, confrontando-a posteriormente com a programação efetiva de três programas de uma das mais tradicionais emissoras segmentadas em jornalismo de São Paulo: a Rádio Eldorado AM. Quanto à metodologia, primeiramente efetuamos uma análise de conteúdo, a fim de compreender a utilização de tais formatos dentro de gêneros estabelecidos e, assim, propor uma nova classificação dos gêneros jornalísticos brasileiros no rádio, conforme o objetivo geral deste estudo. Num segundo momento realizamos o levantamento da ocorrência dos formatos jornalísticos nos três programas radiofônicos analisados. Ao final, concluímos que os gêneros radiojornalísticos não têm sido bem explorados pelos veículos ditos all news e talk and news.(AU)

DnaA-stimulated transcriptional activation of oriλ: Escherichia coli RNA polymerase β subunit as a transcriptional activator contact site

We present evidence that Escherichia coli RNA polymerase β subunit may be a transcriptional activator contact site. Stimulation of the activity of the pR promoter by DnaA protein is necessary for replication of plasmids derived from bacteriophage λ. We found that DnaA activates the pR promoter in vitro. Particular mutations in the rpoB gene were able to suppress negative effects that certain dnaA mutations had on the replication of λ plasmids; this suppression was allele-specific. When a potential DnaA-binding sequence located several base pairs downstream of the pR promoter was scrambled by in vitro mutagenesis, the pR promoter was no longer activated by DnaA both in vivo and in vitro. Therefore, we conclude that DnaA may contact the β subunit of RNA polymerase during activation of the pR promoter. A new classification of prokaryotic transcriptional activators is proposed.

Propuesta de una nueva clasificación de los oligoelementos para su aplicación en nutrición, oligoterapia, y otras estrategias terapéuticas

Objetivos: 1) Proponer una nueva clasificación de los oligoelementos fundamentada en el estudio detallado de las investigaciones más recientes sobre los mismos; 2) ofrecer información detallada y actualizada sobre todos los oligoelementos. Resultados: el análisis de todos los resultados de investigación consultados pone de manifiesto que los avances en las técnicas de análisis molecular permiten dilucidar la importancia que presentan ciertos oligoelementos para la salud humana. Se ofrece un análisis detallado de la función catalítica que podrían tener determinados elementos no considerados hasta ahora como esenciales o posiblemente esenciales, gracias al uso de plataformas informáticas que permiten el análisis integrado de datos sobre enzimas. Asimismo se presenta información integrada y actualizada del papel fisiológico, cinéticas y metabolismo, fuentes dietéticas y factores que propician la carencia o la toxicidad de cada uno de los oligoelementos. Conclusiones: La Oligoterapia plantea el uso de oligoelementos catalíticamente activos con fines terapéuticos. La nueva clasificación de oligoelementos planteada en este trabajo será de interés para diversos sectores profesionales: médicos y demás personal sanitario, nutricionistas, farmacéuticos, etc. Así podrán diseñarse nuevas estrategias terapéuticas que permitan paliar la sintomatología de diversas patologías, en particular las enfermedades carenciales y metabólicas.

GÊNEROS RADIOJORNALÍSTICOS: Análise da Rádio Eldorado de São Paulo

A tese de doutorado insere-se no âmbito da Processos Comunicacionais, tendo como projeto temático a Midiologia Comparada. Tem como objetivo propor uma nova classificação para os gêneros radiojornalísticos, a partir do levantamento e da análise comparativa da bibliografia existente sobre o tema, confrontando-a posteriormente com a programação efetiva de três programas de uma das mais tradicionais emissoras segmentadas em jornalismo de São Paulo: a Rádio Eldorado AM. Quanto à metodologia, primeiramente efetuamos uma análise de conteúdo, a fim de compreender a utilização de tais formatos dentro de gêneros estabelecidos e, assim, propor uma nova classificação dos gêneros jornalísticos brasileiros no rádio, conforme o objetivo geral deste estudo. Num segundo momento realizamos o levantamento da ocorrência dos formatos jornalísticos nos três programas radiofônicos analisados. Ao final, concluímos que os gêneros radiojornalísticos não têm sido bem explorados pelos veículos ditos all news e talk and news.(AU)