Ecological Condition of Coastal Ocean Waters within Stellwagen Bank National Marine Sanctuary: 2008

In June 2008, the NOAA National Ocean Service (NOS), in conjunction with the EPA National Health and Environmental Effects Laboratory (NHEERL), conducted an assessment of the status of ecological condition of soft-bottom habitat and overlying waters within the boundaries of Stellwagen Bank National Marine Sanctuary (SBNMS). The sanctuary lies approximately 20 nautical miles east of Boston, MA in the southwest Gulf of Maine between Cape Ann and Cape Cod and encompassing 638 square nautical miles (2,181 km2). A total of 30 stations were targeted for sampling using standard methods and indicators applied in prior NOAA coastal studies and EPA’s Environmental Monitoring and Assessment Program (EMAP) and National Coastal Assessment (NCA). A key feature adopted from these studies was the incorporation of a random probabilistic sampling design. Such a design provides a basis for making unbiased statistical estimates of the spatial extent of ecological condition relative to various measured indicators and corresponding thresholds of concern. Indicators included multiple measures of water quality, sediment quality, and biological condition (benthic fauna, fish tissue contaminant levels). Depths ranged from 31 – 137 m throughout the study area. About 76 % of the area had sediments composed of sands (< 20 % silt-clay), 17 % of the area was composed of intermediate muddy sands (20 – 80 % silt-clay), and 7 % of the sampled area consisted of mud (> 80 % siltclay). About 70 % of the area (represented by 21 sites) had sediment total organic carbon (TOC) concentrations < 5 mg/g and all but one site (located in Stellwagen Basin) had levels of TOC < 20 mg/g, which is well below the range potentially harmful to benthic fauna (> 50 mg/g). Surface salinities ranged from 30.6 – 31.5 psu, with the majority of the study region (approximately 80 % of the area) having surface salinities between 30.8 and 31.4 psu. Bottom salinities varied between 32.1 and 32.5 psu, with bottom salinities at all sites having values above the range of surface salinities. Surface-water temperatures varied between 12.1 and 16.8 ºC, while near-bottom waters ranged in temperature from 4.4 – 6.2 ºC. An index of density stratification (Δσt) indicated that the waters of SBNMS were stratified at the time of sampling. Values of Δσt at 29 of the 30 sites sampled in this study (96.7 % of the study area) varied from 2.1 – 3.2, which is within the range considered to be indicative of strong vertical stratification (Δσt > 2) and typical of the western Gulf of Maine in summer. Levels of dissolved oxygen (DO) were confined to a fairly narrow range in surface (8.8 – 10.4 mg/L) and bottom (8.5 – 9.6 mg/L) waters throughout the survey area. These levels are within the range considered indicative of good water quality (> 5 mg/L) with respect to DO. None of these waters had DO at low levels (< 2 mg/L) potentially harmful to benthic fauna and fish.

Assessment and monitoring of marine debris in Gray's Reef National Marine Sanctuary

Gray’s Reef National Marine Sanctuary (GRNMS) is located 32.4 km offshore of Sapelo Island, Georgia. The ecological importance of this area is related to the transition between tropical and temperate waters, and the existence of a topographically complex system of ledges. Due to its central location, GRNMS can be used as a focal site to study the accumulation and impacts of marine debris on the Atlantic continental shelf offshore of the Southeast United States. Previously, researchers characterized marine debris in GRNMS and reported that incidence of the debris at the limited densely colonized ledge sites was significantly greater than at sand or sparsely colonized live bottom, and is further influenced by the level of boating activity and physiographic characteristics (e.g., ledge height). Information gleaned from the initial marine debris characterization was used to devise a strategy for prioritizing cleanup and monitoring efforts. However, a significant gap in knowledge was the rate of debris accumulation. The primary objective of this study was to select, mark, and perform initial marine debris surveys at permanent monitoring sites within GRNMS to quantify long-term trends in types, abundance, impacts, and accumulation rates of debris. Ledge sites were selected to compare types, abundance, and accumulation rates of marine debris between a) areas of high and low use and b) short and tall ledges. Nine permanent monitoring sites were marked and initially surveyed in 2007/2008. Surveys were conducted within a 50 x 4 m transect for a total survey area of 200 square meters. All debris was removed and detailed information was taken on the types of debris, quantity, and associations with benthic fauna. Information on associations with benthic fauna included degree of entanglement, type of organism with which it is entangled or resting on, degree of fouling, and visible impacts such as tissue abrasions. Sites were re-surveyed approximately one year later to quantify new accumulation. During the initial survey, a total of ten debris items, totaling 16.3 kg in weight, were removed from two monitoring stations, both “tall” sites within the area of high boat use. Year-one accumulation totaled five items and approximately 7 kg in weight. Similar to the initial survey, all debris was found at sites in the area of high boat use. However, in contrast to the initial survey, two of these items were found on medium-height ledges. Removed items included fishing line, leaders, rope, plastic, and fabric. Although items were often encrusted in benthic biota or entangled on the ledge, impacts such as abrasions or other injuries were not observed. During the 2009 monitoring efforts, volunteer divers were trained to conduct the survey. Monitoring protocols were documented for GRNMS staff and included as an appendix of this report to enable long-term monitoring of sites. Additionally, national reconnaissance data (e.g. satellite, radar, aerial surveys) and other information on known fishing locations were examined for patterns of resource use and correlations with debris occurrence patterns. A previous model predicting the density of marine debris based on ledge features and boat use was refined and the results were used to generate a map of predicted debris density for all ledges.

Environmental and social impact monitoring of the Bujagali Hydropower Project (BHPP), Uganda Fisheries Component: monthly patterns in physico-chemical conditions and fish catch in the immediate vicinity of the Bujagali Hydropower construction site, 29th September - 28th October 2007

Following the commencement of construction works of a 250 MW hydropower plant at Dumbbell Island in the Upper Victoria Nile in September 2007, BEL requested NaFIRRI to conduct continuous monitoring of fish catches at two transects i.e. the immediate upstream transect of the project site (Kalange-Makwanzi) and the immediate downstream .transect (Buyala-Kikubamutwe). The routine monitoring surveys were designed to be conducted twice a week at each of the tWo transects. It was anticipated that major immediate impacts were to occur during construction, and these needed to be known by BEL as part of a mitigation strategy. For example, the construction of it cofferdam could be accompanied by rapid changes in water quality and quantity downstream of the construction. These changes in turn could affect the fish catch and would probably be missed by the quarterly monitoring already in place. Therefore, a major cbjective of the more regular and rapid monitoring was to discern immediate impacts of construction activities by focusing on selected water quality parameters (total suspended solids, water conductivity, temperature, dissolved oxygen and pH) and fish catch characteristics (total catch, catch rates and value of the catch)

Environment monitoring technical report for the SON cage fish culture site at Bugungu, Napoleon Gulf, northern Lake Victoria, November 2013

Source of the Nile Fish farm (SON) is located at Bugungu area in Napoleon Gulf, northern Lake Victoria. The proprietors of the farm and the National Fisheries Resources Research Institute (NaFIRRI) have an established collaborative arrangement where NaFIRRI provides technical back‐stopping to enable quarterly environment monitoring of the cage site as a mandatory requirement of the National Environment Management Authority (NEMA). The agreed study areas are selected physical‐chemical factors (water depth, water transparency/secchi depth, water temperature, dissolved oxygen, pH, conductivity, and nutrient status), algal community (including primary production), aquatic invertebrates (zooplankton and macro‐benthos) and the fish community. This report presents field observations made during the fourth quarter (October‐December) field survey undertaken during December 2013; along with scientific interpretation and discussion of the results in reference to possible impacts of the cage facility to the water environment quality and aquatic biota.

Environment monitoring technical report for the SON cage fish culture site at Bugungu, Napoleon Gulf, northern Lake Victoria, June 2013

Source of the Nile Fish farm (SON) is located at Bugungu area in Napoleon Gulf, northern Lake Victoria. The proprietors of the farm requested NaFIRRI to provide technical assistance to undertake regular environment monitoring of the cage site as a mandatory requirement under the NEMA conditions. NAFIRRI agreed to undertake regular environment surveys in the cage area covering selected physical‐chemical factors (water column depth, water transparency, water column temperature, dissolved oxygen, pH, conductivity, nutrient status), algal aquatic invertebrates (micro‐invertebrates/zooplankton and macro‐benthos) and fish communities. During the year 2013, it was agreed with management to undertake quarterly environment monitoring surveys. However, the first quarter (January‐March 2013) survey was missed out due to late decision. The present report therefore covers the survey taken during the second quarter (April‐June 2013). Results/observations made are presented in this technical report along with a scientific interpretation and discussion of the results with reference to possible impacts of the cage facilities to the water environment and aquatic biota.

National report of the frame survey 2010 on the Uganda side of Lake Victoria

This is a report on the results of the Frame Survey conducted in the Uganda side of Lake Victoria during August 2010 by the LVFO Institutions, namely: the Department of Fisheries Resources (DFR) Uganda and the National Fisheries Resources Research Institute (NaFIRRI) in close collaboration with the District Fisheries offices of Busia, Bugiri, Mayuge, Jinja, Mukono, Kampala, Wakiso, Mpigi, Masaka, Kalangala and Rakai. The authors are grateful to the LVEMP II, for providing funds for the survey and the LVFO secretariat coordination. Fisheries Frame surveys have been carried out on Lake Victoria biannually since 2000 to determine the number of fishers, fish landing sites, and facilities at the landing sites, as well as the composition of fishing crafts, their mode of propulsion, fishing gears and the fish species they target. This information is used to guide development and management of the lake’s fisheries. Following the reorganisation of landing sites into Beach Management Units (BMUs), the number of landing sites decreased from 597 in 2000 to 435 in 2008. The survey in 2010 showed an increase to 503 landing sites, an indication that new landing sites are coming up. The fish landing sites continue to have inadequate facilities such as fish shades, cold rooms to service the fisheries industry and very few (5%) have access to electricity and 32% had access to all weather roads. There has been some progressive improvement in the landing site coverage of basic hygiene and sanitation facilities, especially public toilet facilities from 17% in 2000 to 39% in 2010; and portable water from 4% to 17% respectively. However more effort is required to cover all landing sites. Most landing sites (83%) have access to mobile phone networks which eases communication. 46% of landing sites had access to a Health clinic and 64% had a Primary school within a radius of 2 km.

Bi-annual environment monitoring technical report for the SON cage fish culture site at Bugungu, Napoleon Gulf, northern Lake Victoria, December 2012

The first year-round quarterly surveys were completed for the year 2011. For the year 2012, SON management decided to change the frequency of the surveys from quarterly to biannual and the first such survey, was undertaken in June 2012. The second survey was undertaken in December 2012 and is the subject of this report: Results/observations made are presented in this technical report along with a scientific interpretation and discussion of the results with reference to possible impacts of the cage facilities to the water environment and aquatic biota. SON cage study sites were coded as downstream of cages (DSC), within cages (WIC) and upstream of cages (USC). Physical-chemical parameters (water column temperature, dissolved oxygen, pH, conductivity, were measured in-situ with a pre-calibrated hydrolab at each site. A digital Echo Sounder was used to determine the total water column depth at each site. A black and white Secchi disc was used to determine water column transparency. Coordinate locations were determined with a GPS device.

Environment monitoring technical report for the SON cage fish culture site at Bugungu, Napoleon Gulf, northern Lake Victoria, August 2014

Source of the Nile Fish farm (SON) is located at Bugungu area in Napoleon Gulf, northern Lake Victoria. The proprietors of the farm and the National Fisheries Resources Research Institute (NaFIRRI) have an established collaborative arrangement where NaFIRRI provides technical back-stopping to enable quarterly environment monitoring of the cage site; a mandatory requirement of the National Environment Management Authority (NEMA). The agreed study areas are physical-chemical factors (water depth, water transparency/secchi depth, water temperature, dissolved oxygen, BOO, pH, conductivity), and selected nutrients), algal community (including primary production), aquatic invertebrates (zooplankton and macrobenthos) and the fish community. This report presents field observations made during the third quarter (July-September) field survey undertaken during August 2014; along with scientific interpretation and discussion of the results in reference to possible impacts of the cage facility to the water environment quality and aquatic biota.

Environment monitoring technical report for the SON cage fish culture site at Bugungu, Napoleon Gulf, northern Lake Victoria, September 2013

Source of the Nile Fish farm (SON) is located in northern Lake Victoria close to the headwaters of the River Nile. The proprietors of the farm have established a collaborative agreement with the National Fisheries Resources Research Institute (NaFIRRI) to undertake quarterly environment monitoring surveys of the fish cage site at Bugungu in the Napoleon Gulf. This activity is a mandatory requirement of the National Environment Management Authority (NEMA) of Uganda. Therefore NAFIRRI undertakes monitoring surveys once every quarter covering selected physical‐chemical parameters including water column depth, water transparency, water column temperature, dissolved oxygen, pH, conductivity and nutrient status; algal, zooplankton, macro‐benthos and fish communities. While the first quarter survey of 2013 (January‐March) was missed out due to late decision, the second quarter monitoring survey was dully undertaken in May 2013 and a technical report was compiled and submitted to the client. The present report covers the third quarter survey (July‐September) undertaken in September 2013. Results/observations made are presented in this technical report along with a scientific interpretation and discussion of the results with reference to possible impacts of the cage facilities to the water environment quality and selected aquatic biota.

SON bi‐annual environment monitoring technical report for the cage culture site at Bugungu, northern Lake Victoria, June 2012

Source of the Nile Fish farm (SON) is located at Bugungu area in Napoleon Gulf, northern Lake Victoria. The proprietors of the farm requested NaFIRRI to provide technical assistance to undertake regular environment monitoring of the cage site as is mandatory under the NEMA conditions. NAFIRRI agreed to undertake regular environment surveys in the cage area covering selected physical‐chemical factors i.e. water column depth, water transparency, water column temperature, dissolved oxygen, pH, conductivity, redox potential and turbidity; nutrient status, algal and invertebrate communities (micro‐invertebrates/zooplankton and macro‐invertebrates/macro‐benthos) as well as fish community. The first year‐round quarterly surveys were completed for the year 2011. It was decided by SON management to change the frequency of the monitoring surveys to biannual starting in the year 2012 and the first such survey, which is the subject of this report, was undertaken in June 2012. Results/observations made are presented in this technical report along with a scientific interpretation and discussion of the results with reference to possible impacts of the cage facilities to the water environment and aquatic biota. SON

Environment monitoring technical report for the SON cage fish culture site at Bugungu, Napoleon Gulf, northern Lake Victoria, March 2014

Source of the Nile Fish farm (SON) is located at Bugungu area in Napoleon Gulf, northern Lake Victoria. The proprietors of the farm and the National Fisheries Resources Research Institute (NaFIRRI) have an established collaborative arrangement where NaFIRRI provides technical back‐stopping to enable quarterly environment monitoring of the cage site as a mandatory requirement of the National Environment Management Authority (NEMA). The agreed study areas are selected physical‐chemical factors (water depth, water transparency/secchi depth, water temperature, dissolved oxygen, pH, conductivity, and nutrient status), algal community (including primary production), aquatic invertebrates (zooplankton and macro‐benthos) and the fish community. This report presents field observations made during the first quarter (January‐March) field survey undertaken during March 2014; along with scientific interpretation and discussion of the results in reference to possible impacts of the cage facility to the water environment quality and aquatic biota. The

Symmetrical 1-pyrrolidineacetamide showing anti-HIV activity through a new binding site on HIV-1 integrase

Aim: To characterize the functional and pharmacological features of a symmetrical 1-pyrrolidineacetamide, N,N'-(methylene-di-4,1-phenylene) bis-1-pyrrolidineacetamide, as a new anti-HIV compound which could competitively inhibit HIV-1 integrase (IN) bindi

Report of the baseline survey undertaken on the Chinese cage site in Napoleon Gulf, Northern Lake Victoria, 12 March 2012

The government of the People's Republic of China through a 2007 agreement with the Government of the Republic of Uganda, has establishment of an Agricultural Technology Demonstration Center (ATDC). The first phase covering the building of aquaculture infrastructure at Kajjansi ARDC is complete and the second operation phase has started in which facilities for cage culture have been set up in the Napoleon gulf, northern Lake Victoria near Jinja. The cage facility is aimed at boosting fish farming within the lake as a diversification to the traditional pond fish culture technology. NaFIRRI scientists as well as Chinese experts undertook a baseline survey in the chosen cage site on 12 March 2012. The survey covered determination of water depth, water transparency, measurement of selected physical-chemical parameters (temperature,dissolved oxygen, conductivity and pH; determination of the nutrient status and study of algae, invertebrate and fish communities at the site. Materials and methodologies used in the survey were based on the Standard Operating Procedures (SOPs) of NaFIRRI. The study area was divided into three study sites. Site 1 (upstream) was at 8.9 metre depth while site 2 (proposed cage site) and site 3 (downstream) were 6 and 4.3 metres deep respectively. Water transparency was lowest at site 1 (1.58 m) and highest at site 3 (1.64 m). Dissolved oxygen at the three sites ranged from 6.0 to 8 mg/I. Water temperature profiles fluctuated within narrow limits between 26.5 and 27.5 DC. Measurements of pH were between 7 (neutral) and 8 (alkaline) while electrical conductivity was between 98 and 101 uS/em. These observed physical-chemical parameters at the study site were considered suitable for cage fish rearing purposes. Nitrite-nitrogen levels varied within narrow limits from 0.043 to 0.0453 mgtl. Similarly, Ammonia-nitrogen varied between 0.015 and 0.0185 mg/1. Soluble reactive phosphorus (SRP) level was highest at site 3 (O.012mgll) compared to that at sites 1 and 2 (0.009mgll). Total suspended solids (TSS) were higher at site 1 (83.3mgll), thereafter decreasing to lower levels at sites 2 (24.8mgtl) and 3 (19.8mgl) respectively. The nutrient level results observed here all fall below the maximum permissible limits by NEMA and therefore the site is recommended for cage culture The algal community was constituted by four major groups: Blue greens,Greens, Cryptophytes, and Diatoms with blue greens as the common and dominant group. High algal biomass (19944961 ugtL) of the dominant blue green algae was observed at site 1 compared site 2 and 3 (58655.2 & 27487. 7 ugtL) respectively. Occurrence of toxicin producing algae: microsytis and cylindrospermopsis in the proposed cage area was considered to be of not much significance as their concentrations were below harmful levels. However, monitoring their presence, biomass and seasonality will be critical in order to follow when and where they occur and at what time of the year for ease of management of the cages

Monthly patterns in physico-chemical conditions and fish catch in the immediate vicinity of the Bujagali hydropower construction site 29th September - 28th October 2007

The specific objectives were to: WATER QUALITY 1. To measure the water physical variables as indicators of environmental conditions in the upstream and downstream transects of Kalange (1) and Buyala (2), respectively, 2. To determine the concentrations of total suspended solids as a major constituent likely to be released into the waters at any time during the construction activities, by comparing the concentrations at the two transects. FISH CATCH 1. To follow up trends in fish catch as construction activity progresses, and to precision of the estimate; 2. To estimate the prevailing fish catch rates, total fish catches and the total value of the fish catch to the local fishers at the two transects.

Classical two-site fidelity and quantum phase transitions in the Ising model and the extended Hubbard model

In this Letter, the classical two-site-ground-state fidelity (CTGF) is exploited to identify quantum phase transitions (QPTs) for the transverse field Ising model (TFIM) and the one-dimensional extended Hubbard model (EHM). Our results show that the CTGF exhibits an abrupt change around the regions of criticality and can be used to identify QPTs in spin and fermionic systems. The method is especially convenient when it is connected with the density-matrix renormalization group (DMRG) algorithm. (C) 2008 Elsevier B.V. All rights reserved.