Serological identification of house dust mite allergens in dogs with atopic dermatitis

House dust mite antigens have been used for decades to diagnose allergic diseases in humans and animals. The objective of this study was to identify allergens in commercial Dermatophagoides farinae and Blomia tropicalis extracts by immunoblotting using sera from allergic dogs and anti-dog IgE conjugate. The analysis of antigens present in the D. farinae extract (FDA Allergenic) using sera from 10 dogs allergic to D. farinae showed that eight sera recognized a band of approximately 102 kDa, eight recognized two bands of 52 to 76 kDa, five recognized one band of approximately 76 kDa, four recognized one band of 31 to 38 kDa, and two recognized one band of 12 to 17 kDa. Immunoblot assays of the B. tropicalis extract (FDA Allergenic) using sera from 10 animals allergic to B. tropicalis showed that five sera recognized two bands of 52 to 76 kDa. These results demonstrate the importance of the two house dust mite species for the pathogenesis of canine atopic dermatitis in Brazil. In addition, the results indicate which allergens should be present in allergenic extracts used for diagnosis and allergen-specific immunotherapy.

IgA response in serum and gut secretion in sensitized mice fed with the dust mite Dermatophagoides pteronyssinus extract

Induced oral tolerance to mucosal-exposed antigens in immunized animals is of particular interest for the development of immunotherapeutic approaches to human allergic diseases. This is a unique feature of mucosal surfaces which represent the main contact interface with the external environment. However, the influence of oral tolerance on specific and natural polyreactive IgA antibodies, the major defense mechanism of the mucosa, is unknown. We have shown that oral administration of an extract of the dust mite Dermatophagoides pteronyssinus (Dp) to primed mice caused down-regulation of IgE responses and an increase in tumor growth factor-ß secretion. In the present study, we observed that primed inbred female A/Sn mice (8 to 10 weeks old) fed by gavage a total weight of 1.0-mg Dp extract on the 6th, 7th and 8th days post-immunization presented normal secretion of IL-4 and IL-10 in gut-associated lymphoid tissue and a decreased production of interferon gamma induced by Dp in the draining lymph nodes (13,340 ± 3,519 vs 29,280 ± 2,971 pg/ml). Mice fed the Dp extract also showed higher levels of serum anti-Dp IgA antibodies and an increase of IgA-secreting cells in mesenteric lymph nodes (N = 10), reflecting an increase in total fecal IgA antibodies (N = 10). The levels of secretory anti-Dp IgA antibodies increased after re-immunization regardless of Dp extract feeding. Oral tolerance did not interfere with serum or secretory IgA antibody reactivity related to self and non-self antigens. These results suggest that induction of oral tolerance to a Dp extract in sensitized mice triggered different regulatory mechanisms which inhibited the IgE response and stimulated systemic and secretory IgA responses, preserving the natural polyreactive IgA antibody production.

Cloning, sequence analysis, and expression of cDNA coding for the major house dust mite allergen, Der f 1, in Escherichia coli

Our objective was to clone, express and characterize adult Dermatophagoides farinae group 1 (Der f 1) allergens to further produce recombinant allergens for future clinical applications in order to eliminate side reactions from crude extracts of mites. Based on GenBank data, we designed primers and amplified the cDNA fragment coding for Der f 1 by nested-PCR. After purification and recovery, the cDNA fragment was cloned into the pMD19-T vector. The fragment was then sequenced, subcloned into the plasmid pET28a(+), expressed in Escherichia coli BL21 and identified by Western blotting. The cDNA coding for Der f 1 was cloned, sequenced and expressed successfully. Sequence analysis showed the presence of an open reading frame containing 966 bp that encodes a protein of 321 amino acids. Interestingly, homology analysis showed that the Der p 1 shared more than 87% identity in amino acid sequence with Eur m 1 but only 80% with Der f 1. Furthermore, phylogenetic analyses suggested that D. pteronyssinus was evolutionarily closer to Euroglyphus maynei than to D. farinae, even though D. pteronyssinus and D. farinae belong to the same Dermatophagoides genus. A total of three cysteine peptidase active sites were found in the predicted amino acid sequence, including 127-138 (QGGCGSCWAFSG), 267-277 (NYHAVNIVGYG) and 284-303 (YWIVRNSWDTTWGDSGYGYF). Moreover, secondary structure analysis revealed that Der f 1 contained an a helix (33.96%), an extended strand (17.13%), a ß turn (5.61%), and a random coil (43.30%). A simple three-dimensional model of this protein was constructed using a Swiss-model server. The cDNA coding for Der f 1 was cloned, sequenced and expressed successfully. Alignment and phylogenetic analysis suggests that D. pteronyssinus is evolutionarily more similar to E. maynei than to D. farinae.

Cloning, bioinformatics analysis, and expression of the dust mite allergen Der f 5 of Dermatophagoides farinae

Crude extracts of house dust mites are used clinically for diagnosis and immunotherapy of allergic diseases, including bronchial asthma, perennial rhinitis, and atopic dermatitis. However, crude extracts are complexes with non-allergenic antigens and lack effective concentrations of important allergens, resulting in several side effects. Dermatophagoides farinae (Hughes; Acari: Pyroglyphidae) is one of the predominant sources of dust mite allergens, which has more than 30 groups of allergen. The cDNA coding for the group 5 allergen of D. farinae from China was cloned, sequenced and expressed. According to alignment using the VECTOR NTI 9.0 software, there were eight mismatched nucleotides in five cDNA clones resulting in seven incompatible amino acid residues, suggesting that the Der f 5 allergen might have sequence polymorphism. Bioinformatics analysis revealed that the matured Der f 5 allergen has a molecular mass of 13604.03 Da, a theoretical pI of 5.43 and is probably hydrophobic and cytoplasmic. Similarities in amino acid sequences between Der f 5 and allergens of other domestic mite species, viz. Der p 5, Blo t 5, Sui m 5, and Lep d 5, were 79, 48, 53, and 37%, respectively. Phylogenetic analysis indicated that Der f 5 and Der p 5 clustered together. Blo t 5 and Ale o 5 also clustered together, although Blomia tropicalis and Aleuroglyphus ovatus belong to different mite families, viz. Echimyopodidae and Acaridae, respectively.

Exposición suMaría del sistema frenológico del doctor Gall

UANL

MCMI-II borderline personality disorder in anorexia and bulimia nervosa. 'Trastorno l??mite de personalidad seg??n el MCMI-II en la anorexia y bulimia nerviosas'

Resumen tomado de la publicaci??n

Sentit pedagògic del mite de la bèstia enamorada. 'Sentido pedagógico del mito de la bestia enamorada'.

El tema de la bestia enamorada es frecuente en los cuentos de todas las culturas. No siempre se trata de un animal sino que el enamorado suele adoptar formas mostruosas y feroces. Partiendo del psicoanálisis, se ha demostrado la acción liberadora que ejercen los cuentos populares en la formación de la personalidad infantil y en la maduración de la afectividad, el enriquecimiento del lenguaje y la imaginación, la educación de la atención pero sobre todo la capacidad que poseen para favorecer el conocimiento y superar los propios problemas emocionales a través de recrearlos en los cuentos. El coraje es sin duda uno de los valores que con más insistencia ofrecen los cuentos populares: la aventura iniciática es siempre un reto, un riesgo a los ojos del protagonista para que sea capaz de correr las peripecias llenas de intriga que la historia le plantea. Pero sobre todo lo que es más destacable en los cuentos es que sus mensajes llegan a través de simples y cotidianas situaciones sociales, como el mito de la bestia enamorada sirve para cambiar la actitud de la mujer frente al sexo, para pasar del sentimiento de rechazo y miedo a la aceptación.

Sentit pedagògic del mite de la bèstia enamorada. 'Sentido pedagógico del mito de la bestia enamorada'.

Resumen tomado de la publicación

El Montseny i les Guilleries : paisatge, mite i literatura. 'El Montseny y las Guilleries : paisaje, mito y literatura'

Resumen del v??deo en catal??n

Mitologia : l'antropologia a través del mite. 'Mitología : la antropología a través del mito'.

Trabajo que analiza los mitos y las leyendas que han creado los distintos pueblos y culturas del mundo en su intento de dar respuesta a las preguntas que a lo largo de los siglos se ha planteado la humanidad. Asi el capítulo segundo estudia los dioses y héroes de la mitología china, africana, árabe, inca, vikinga y helénica. Para finalizar con una cronología, documentos y bibliografía, así como con una serie de actividades y juegos para una mejor comprensión del tema..

El CAP : tr??mite y exigencia

El matricularse en el CAP es simplemente un tr??mite burocr??tico y obligatorio. Exigible para dar clases y opositar. Est?? condenado a vivir sin ser curso de aptitud pedag??gica. Si este curso es necesario y requiere una organizaci??n hay que organizarlo bien, con profesores competentes que supervisen de modo constante las practicas, programaciones y resultados de su actividad. La memoria es un tr??mite es un tramite molesto e innecesario que se deber??a suprimir. La ruina del CAP no viene de ensayar f??rmulas nuevas, sino de querer mantener a toda costa una estructura caduca y cara, adem??s de inoperante e in??til.

Definiciones personales y aspectos estructurales del concepto de l??mite finito de una funci??n en un punto

Resumen basado en el de la publicaci??n

La regulaci??n de las titulaciones universitarias oficiales como??l??mite a la descentralizaci??n en la Educaci??n Superior

Resumen basado en el de la publicaci??n