Differentially regulated NADPH:cytochrome P450 oxidoreductases in parsley

Two NADPH:cytochrome P450 oxidoreductases (CPRs) from parsley (Petroselinum crispum) were cloned, and the complete proteins were expressed and functionally identified in yeast. The two enzymes, designated CPR1 and CPR2, are 80% identical in amino acid sequence with one another and about 75% identical with CPRs from several other plant species. The mRNA accumulation patterns for CPR1 and CPR2 in fungal elicitor-treated or UV-irradiated cultured parsley cells and in developing or infected parsley plants were compared with those for cinnamate 4-hydroxylase (C4H), one of the most abundant CPR-dependent P450 enzymes in plants. All treatments strongly induced the mRNAs for C4H and CPR1 but not for CPR2, suggesting distinct metabolic roles of CPR1 and CPR2 and a functional relationship between CPR1 and C4H.

Regulation and Functional Expression of Cinnamate 4-Hydroxylase from Parsley

A previously isolated parsley (Petroselinum crispum) cDNA with high sequence similarity to cinnamate 4-hydroxylase (C4H) cDNAs from several plant sources was expressed in yeast (Saccharomyces cerevisiae) containing a plant NADPH:cytochrome P450 oxidoreductase and verified as encoding a functional C4H (CYP73A10). Low genomic complexity and the occurrence of a single type of cDNA suggest the existence of only one C4H gene in parsley. The encoded mRNA and protein, in contrast to those of a functionally related NADPH:cytochrome P450 oxidoreductase, were strictly coregulated with phenylalanine ammonia-lyase mRNA and protein, respectively, as demonstrated by coinduction under various conditions and colocalization in situ in cross-sections from several different parsley tissues. These results support the hypothesis that the genes encoding the core reactions of phenylpropanoid metabolism form a tight regulatory unit.

(Table 1) Gravity and pressure within the Sleipner area relative to ones at the reference station in 2002 and 2005

At Sleipner, CO2 is being separated from natural gas and injected into an underground saline aquifer for environmental purposes. Uncertainty in the aquifer temperature leads to uncertainty in the in situ density of CO2. In this study, gravity measurements were made over the injection site in 2002 and 2005 on top of 30 concrete benchmarks on the seafloor in order to constrain the in situ CO2 density. The gravity measurements have a repeatability of 4.3 µGal for 2003 and 3.5 µGal for 2005. The resulting time-lapse uncertainty is 5.3 µGal. Unexpected benchmark motions due to local sediment scouring contribute to the uncertainty. Forward gravity models are calculated based on both 3D seismic data and reservoir simulation models. The time-lapse gravity observations best fit a high temperature forward model based on the time-lapse 3D seismics, suggesting that the average in situ CO2 density is about to 530kg/m**3. Uncertainty in determining the average density is estimated to be ±65 kg/m**3 (95% confidence), however, this does not include uncertainties in the modeling. Additional seismic surveys and future gravity measurements will put better constraints on the CO2 density and continue to map out the CO2 flow.

Zwei musikdichtungen: Die verlorenen kronen. Die Totentrud.

Mode of access: Internet.

Methods of measuring magnetic fields

Mode of access: Internet.

Fornalder sögur nordrlanda eptir gömlum handritum utgefnar af C. C. Rafn.

1. bd. Saga af Hrólfi konúngi Kraka. Völsúnga saga. Saga af Ragnari konúngi lodbrók ok sonum hans. Krákumál. Söguþáttr af Norna-Gesti. Þáttr af Ragnars sonum. Sögubrut af nokkrum fornkonúngum. Sörla Þáttr. Hervarar saga ok Heidreks konúngs.--2. bd. Frá Fornjóti ok hans aettmönnum. Saga af Hálfi ok Hálfsrekkum. Fridþjófs saga ens fraekna. Af uplendínga konúngum. Saga Ketils haengs. Saga Grims lodinkinna. Örvar-Odds saga. Áns saga bogsveigis. Saga af Hrómundi Greipssyni. Saga Þorsteins Víkíngssonar. Ásmundar saga kappabana.--3. bd. Saga Gautreks konúngs. Saga ag Hrólfi konungi Gautrekssyni. Saga Herrauðs ok Bósa. Gaungu-Hrólfs saga. Saga Egils ok Asmundar. Sörla saga sterka. Hjalmters ok Ölvers saga. Halfdanar saga Eysteinssonar. Halfdanar saga Brönufóstra. Sturlaugs saga starfsama. Illuga saga Grídarfostra. Ereks saga vídförla.

Isländische litteratur und geschichte.

No more published.

Epic and saga ...

Beowulf.--The song of Roland.--The destruction of Dá Derga's hostel.--The story of the Volsungs and Niblungs.--Songs from the Elder Edda.

School of Nursing Class of 1996

Top Row: Jessica M. Adair, Casey Arnett, Amy Lynn Babchek, Mary E. Bartlett, Rhonda Bass, Nancy Bidlack, Heather Bjerke, Stacy Bodrie, Dana Boonstra, Kellu Bowers, Pamela Bowser, Rachel L. Bradley, Michele Brotherton, Stacie Buckler, Hope Bufkin

Row 2: Saran Burnley, Jennifer Caraan, Barbara Carpenter, Nutrena Helene Watts, Aimee Schuman, Debra Jameson, Jennifer Jennings, Mary Cassette, Nikki Burns, Lisa Multhaupt, Jeffrey M. Adams, Christine Hepner, Julie Chamberlain, Andy Chan, Jennifer Choike

Row 3: Heather Chrisman, Abbey C. Clark, Renita Cobb, Amy Cotton, Cattleya Crossen, Kimberly Curl, Christy Debolt, Patricia DeLamielleure, Jennifer Dyer, Lisa L. Eliasom, Patricia Fowler Faling, Rita Fallone

Row 4: Richard Fisher, Rebecca J. Forbes, Tiffany Fowler, Karen R. Fritz, Debbie M. Fulton, Michelle J. Gaskill, Ellen M. Gavin, Emily Golin, Umeika Makita Griffith, Lydia D. Hampton, Natalie Michele Hoffman, Julie Holbird

Row 5: Kathryn A. Huffman, Tara Lynn Humphrey, Nicole Jaccques, Michelle C. Johnson, Bryan Wayne Kerridge, Violet H. Barkauskas, Beverly Jones, Ada Sue Hinshaw, Nola Pender, Susan Boehm, Noelle Kirouac, Sarah Kohn, Sherri Krajenta, Brian Kubinski, Stephanie L. Kuczera

Row 6: Heather Lange, Sang Hee Lee, Soya Lee, Natalie Lehrer, Kimberly Lilley, Elizabeth A. Lundy, Darcey Lutz-Guenther, Michelle J. Malicsi, Dawn Marteeny, Sheila Mendiola, Sharon Mitchell, Caryl S. Molton, Colette Montilla, Celeste Montone-Horne, Emily T. Mooney, Naima Moore

Row 7: Kami Nobis, Thresa M. Nugent, Michelle Ober, Nisha Patel, Stephanie Perrett, Holly Powers, Julie L. Pryor, Elizabeth K. Rachubinski, Anne Rammelkamp, Kathy Rarog, Erin Richards, Amy Roehrig, Catherine Ann Rosloniec, Tansey Rosset, Kimberly Sanders, Marla Sands, James C. Sausser

Row 8: Juana Sebree, Erin J. Showers, Prabhjyot Singh, Lynn Sinkel, Nicole LaDon Smith, Nicole M. Speck, Mickie Speers, Krista Stapleton, Karon Starr, Elizabeth Studley, Janice Brenda Supena, Rashelle Talbert,Kimberly Tocco, Edda Toting, Lisa Uren, Lori VanBergen

Row 9: Lisa VanStratton, kathleen Veenstra, Kristen Venadam, Rhonda E. Walkowe, Ching-Ru Bonny Wang, Deborah Webb, Ruthann Clausen Weiss, Debra R. White, Rochelle Whiteman, Tara Wilson, Jessica Wise, Sheryl Woloskie, Denice Annette Zakalata, Rebecca S. Zeiler

Authorship Attribution with Support Vector Machines

In this paper we explore the use of text-mining methods for the identification of the author of a text. We apply the support vector machine (SVM) to this problem, as it is able to cope with half a million of inputs it requires no feature selection and can process the frequency vector of all words of a text. We performed a number of experiments with texts from a German newspaper. With nearly perfect reliability the SVM was able to reject other authors and detected the target author in 60–80% of the cases. In a second experiment, we ignored nouns, verbs and adjectives and replaced them by grammatical tags and bigrams. This resulted in slightly reduced performance. Author detection with SVMs on full word forms was remarkably robust even if the author wrote about different topics.

Organic reactions in ionic liquids:Gewald synthesis of 2-aminothiophenes catalyzed by ethylenediammonium diacetate

Ionic liquids based on 1-butyl-3-methylimidazolium tetrafluoroborate (BmimBF4) and 1-butyl-3-methylimidazolium hexafluorophosphate (BmimPF6) were used as reusable alternatives to volatile organic solvents (VOCs) for ethylenediammonium diacetate (EDDA) catalyzed Gewald synthesis of 2-aminothiophenes. Significant rate enhancement and improvement of the yield were observed. The ionic liquids containing catalyst EDDA were recycled several times with no decreases in yields and reaction rates.

Organic reactions in ionic liquids: Knoevenagel condensation catalyzed by ethylenediammonium diacetate

The moisture and air stable ionic liquids 1-butyl-3-methylimidazonium tetrafluoroborate [bmim]BF4 and 1-butyl-3-methylimidazonium hexafluorophosphate [bmim]PF6 were used as ‘green' recyclable alternatives to volatile organic solvents (VOCs) for ethylenediammonium diacetate (EDDA) catalyzed Knoevenagel condensation between aldehydes or ketones with active methylene compounds. Both aldehydes and ketones gave satisfactory results. The ionic liquids containing catalyst EDDA were recycled several times with no decreases in yields and reaction rates. In the case of 2-hydroxybenzaldehyde, the reactions led to the formation of 3-substituted coumarins under standard reaction conditions.

The magical transformation contest in the ancient storytelling tradition

El concurso de transformación mágica, esquema narrativo difundido en la tradición popular, se presenta en dos variantes principales: los hechiceros que compiten pueden metamorfosearse en varios seres o crear esos seres por medios mágicos. En cualquier caso el concursante ganador da a luz criaturas más fuertes que superan las de su oponente. La segunda variante fue preferida en el antiguo Cercano Oriente (Sumeria, Egipto, Israel). La primera se puede encontrar en algunos mitos griegos sobre cambiadores de forma (por ejemplo, Zeus y Némesis). El mismo esquema narrativo puede haber influido en un episodio de la Novela de Alejandro (1.36-38), en el que Darío envía regalos simbólicos a Alejandro y los dos monarcas enemigos ofrecen contrastantes explicaciones de ellos. Esta historia griega racionaliza el concurso de cuento de hadas, transfiriendo las fantásticas hazañas de creaciones milagrosas a un plano secundario pero realista de metáfora lingüística.