A fast method using a new hydrophilic–lipophilic balanced sorbent in combination with ultra-high performance liquid chromatography for quantification of significant bioactive metabolites in wines

This manuscript describes the development and validation of an ultra-fast, efficient, and high throughput analytical method based on ultra-high performance liquid chromatography (UHPLC) equipped with a photodiode array (PDA) detection system, for the simultaneous analysis of fifteen bioactive metabolites: gallic acid, protocatechuic acid, (−)-catechin, gentisic acid, (−)-epicatechin, syringic acid, p-coumaric acid, ferulic acid, m-coumaric acid, rutin, trans-resveratrol, myricetin, quercetin, cinnamic acid and kaempferol, in wines. A 50-mm column packed with 1.7-μm particles operating at elevated pressure (UHPLC strategy) was selected to attain ultra-fast analysis and highly efficient separations. In order to reduce the complexity of wine extract and improve the recovery efficiency, a reverse-phase solid-phase extraction (SPE) procedure using as sorbent a new macroporous copolymer made from a balanced ratio of two monomers, the lipophilic divinylbenzene and the hydrophilic N-vinylpyrrolidone (Oasis™ HLB), was performed prior to UHPLC–PDA analysis. The calibration curves of bioactive metabolites showed good linearity within the established range. Limits of detection (LOD) and quantification (LOQ) ranged from 0.006 μg mL−1 to 0.58 μg mL−1, and from 0.019 μg mL−1 to 1.94 μg mL−1, for gallic and gentisic acids, respectively. The average recoveries ± SD for the three levels of concentration tested (n = 9) in red and white wines were, respectively, 89 ± 3% and 90 ± 2%. The repeatability expressed as relative standard deviation (RSD) was below 10% for all the metabolites assayed. The validated method was then applied to red and white wines from different geographical origins (Azores, Canary and Madeira Islands). The most abundant component in the analysed red wines was (−)-epicatechin followed by (−)-catechin and rutin, whereas in white wines syringic and p-coumaric acids were found the major phenolic metabolites. The method was completely validated, providing a sensitive analysis for bioactive phenolic metabolites detection and showing satisfactory data for all the parameters tested. Moreover, was revealed as an ultra-fast approach allowing the separation of the fifteen bioactive metabolites investigated with high resolution power within 5 min.

Formação de professores, saberes, reflexividade e apropriação da cultura digital no Projeto Um Computador por Aluno (UCA)

This study investigates teacher training and cognitive practice of teachers in a Basic Education school that adopted the Project One Computer per Student (OCS) in their school routine. Its relevance consists in provide directions for the continuation of training activities on the Project and guide the teachers with their pedagogical practices using the laptop model one to one. The thesis defended is that the educator formation for social using of digital media (specially the laptops from the Project UCA) gives space to establish new sociotechnical relationships, of new social and professionals practices, new identitary components and a process of reflexivity and knowledge reconstruction to teach. We reaffirm the importance of reflexivity and appropriation of digital culture for the better development of teaching practice using the Information and Communication Technologies (ICTs), giving focus to the aspects of social and professional use of the technology. The study is part of the qualitative aspect and is a procedural tracking based on principles of ethnographic research. As procedures and methodological tools, were used: intensive observation of school environments, documental analysis, focal group, semi-structured questionnaires and semi-structured individual interviews. The research was held in a public school in the city of Parnamirim - RN. The subject sample relates to 17 teachers, coming from the elementary school I and II, Youth and Adult Education and High School, who went through the process of training UCA and having entered the laptops in their teaching. The research corpus is structured based on the messages built into the process of data collection and is analyzed based on principles of Content Analysis, specified by Laurence Bardin (2011). Was taken as theoretical reference studies by Tardif (2000; 2011), Pimenta (2009), Gorz (2004, 2005), Giddens (1991), Dewey, J. (1916), Boudieu (1994; 1999), Freire (1996; 2005), among others. The analysis indicates a process of reconstruction / revision of knowledge to teach and work in digital culture, being these knowledges guided by the experience of the subjects investigated. The reconstructed knowledges will be revealed from a categorization process. The following groups of knowledges: "technical knowledges", "didactic-methodological knowledges and knowledges of professionalization" were built on the assumption of ownership of digital culture in the educational context. The analysis confirms the appearance of new ways of sociability when acquiring other forms of acting and thinking ICTs, despite the environment adverse to the reflexivity shared among the teachers. Also reveals, based on the ownership concept present on the data analysis, the construction of meanings of belonging and transformation of individuals into social routes from the interweaving of the teaching practice with the digital culture. Emphasizes, finally, the importance of a training for use of ICTs that exceeds the instrumentation, in other words, what we call "technical knowledges", but taking on its structural basis the shared reflection, the opening for the ressignificance (new meaning) and reconstruction of new knowledges and practices and that really allows, to the teacher, the living of an experience capable of providing socio-technical transformations of their relationships

Diagnóstico geoambiental com uso do geoprocessamento visando a determinação de potencialidades de uso do solo para a área estuarina do Rio Curimataú - Canguaretama(RN)

The activities developed in the coastal zone always arise the researchers interest, mainly when it is developed in fragile ecological areas, not observing the sustenable development laws. In this context, the analysis presented developed around the estuary of Curimatau river, located in the Eastern coast of Rio Grande do Norte state, integrates information provided by high resolution images of orbital remote sensing (IKONOS II, dated of July of 2003) and images of not-orbital remote sensing (Air photographs of DPU, dated of March of 1997) in a multitemporary perspective, diagnosising patterns of use and occupation, evaluating the main area´s impacts, together with the estuarian region fisiograph of Curimatau river. The use of high resolution images consequently allowed generates a precision cartography, facilitating the quantification of the evolution of the landscape and supporting decisions. Was possible yet, the definition of use areas, conservation areas and preservation areas; that were inserted in the concept of Potentialities: a fundamental element to management of vulnerable areas of the natural point of view. The information provided during the research had been stored in an Enviromental Georreferenced Data Base, constructed under the optics of Geographical Information Systems (GIS), that they facilitate the environment management Through fast access to information. The results were satisfactory, because permits the quantification of evolution of the landscape and the mapping. Beyond the geoenviromental diagnosis, was possible develop maps and matrixes that support the Rio Grande do Norte state costal Management.

De baixo para cima: implicações socioespaciais da transferência da feira livre na (re)produção do espaço urbano de Nova Cruz-RN (1991-2010)

The present work is about the reproduction of the urban area of Nova Cruz RN, with the objective of analyzing the social and spatial implications of the relocation of the main street market in the city from 1991 on. Nowadays, the city of Nova Cruz takes over importance in the potiguar social and spatial scenario due to its condition of central city which is practiced for decades in Potiguar Agreste Microregion. Beginning with its formation process connected with traveler‟s hostel which assisted the activity of cattle transportation, further in time by the golden era of cotton production in its territory, it was noticed that its importance in relation with the nearby towns has been happening differently throughout time. From the year 1991 on, the town of Nova Cruz has gone through a territory restructuring of its urban area due to the relocation of the open market from Downtown neighborhood to São Sebastião neighborhood. Such territorial movement resulted in a reformation of the town‟s urban space, promoting in both neighborhoods urban growth and the expansion of commercial activity associated with the migration of the commercial center of the city. The transference of the commercial area has caused these processes through the new uses of the land towards the São Sebastião region, it has also caused a decrease in market value of the downtown area as a result of the breakdown of previous existing business activities, their service contribution and the citizen migration, establishing a space and socioeconomic portrait in the neighborhood. From this context, our analysis seeks to understand the social and spatial impacts occurred in Downtown neighborhood, the way in which the production and reproduction of the urban space in São Sebastião neighborhood and the implications resulted from the actions of the administrative power in restructuring of the urban space of Nova Cruz. In order to do so, a bibliographical research was used to compose our theoretic-conceptual mark, discussing the matter with authors such as Roberto Lobato Corrêa, Ana Fani, Milton Santos, Manuel Castells e Heri Lefvbre, among others, discussing on the subject of urban analysis, the concepts of urban space and the process of reproduction of the urban space. A field research was utilized in our area of study by means of primary data gathering through interviews, questionnaire and photographic register. Secondary data gathering was also obtained by means of bibliographical and documental research related to our study object. By these means, it was sought to contribute to the understanding of the urban space through its production and reproduction based upon the discovery of social and spatial practices

Era uma vez... contos de fadas e psicodrama auxiliando alunos na conclusão do curso médico

O término do curso representa um período especialmente estressante para estudantes de Medicina, colocando-os perante diversas angústias: deixar de ser aluno, ter novas responsabilidades e enfrentar o exame de residência. Com o intuito de auxiliá-los nesta fase, foi desenvolvida na Faculdade de Medicina de Botucatu (FMB) uma série de estratégias de acolhimento para os estudantes. Este trabalho descreve uma atividade na qual se utilizou o Psicodrama como facilitador da expressão dos sentimentos e emoções experimentadas ao final do curso. Por dois anos consecutivos foram realizadas sessões de Sociodrama com o conjunto dos alunos do 6º ano do curso médico da FMB. Utilizaram-se Contos de Fadas como recurso para que os estudantes identificassem sua trajetória na instituição e o momento que estavam vivendo. A dramatização dos contos possibilitou a troca de experiências entre os alunos e o acolhimento de suas angústias, muitas delas coletivas e próprias daquele contexto. A análise dos contos privilegiou aspectos projetivos grupais, concluindo na direção da necessidade de mais espaços de encontro e troca entre professores e alunos.

Análise do conteúdo de propagandas de medicamentos psicoativos

O objetivo do estudo foi descrever as figuras humanas retratadas nas propagandas de medicamentos psicoativos quanto ao gênero, a idade, a etnia e o contexto social. Foi realizada análise de conteúdo de 86 impressos publicitários inéditos divulgados em Araraquara (SP) no ano de 2005. A associação entre as categorias foi analisada usando o teste exato de Fisher. Houve predomínio de mulheres (62,8%), sendo quatro vezes mais freqüentes que os homens em propagandas de antidepressivos e ansiolíticos. A maioria era constituída de jovens adultos (72%), de etnia branca (98,8%). As pessoas estavam em lazer (46,5%), em suas casas (29%) ou em contato com a natureza (16,2%). A mensagem transmitida foi que os medicamentos tratam sintomatologias subjetivas de desconforto do dia-a-dia, induzindo a um apelo irracional que pode refletir na prescrição medicamentosa.

Expanding the coverage of pension systems in Latin America

Includes bibliography

Projeto trilha de letras: uma análise do software como recurso didático no processo ensino-aprendizagem da leitura e da escrita

Pós-graduação em Educação - FCT

Proposta de uma abordagem metodológica para analisar como os fatores posicionais influenciam as prioridades competitivas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A gramática em contexto teletandem e em livros didáticos de português como língua estrangeira

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A construção de espaço e cultura: trajetórias migratórias entre Monte Azul (MG) e Rio Claro (SP)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Educação do aluno com autismo: um estudo circunstanciado da experiência escolar inclusiva e as contribuições do currículo funcional natural

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Educação rural e desenvolvimento local sustentável: a lógica subjacente das relações inter-setoriais

Este estudo tem por objetivo avaliar os modelos de educação gerados nos processos de ocupação da terra nos municípios Juazeiro, Uauá e Valente no Estado da Bahia, tanto nas áreas irrigadas quanto nas de sequeiro, tendo como hipótese norteadora a educação rural como mecanismo disseminador de informações à população e grande mobilizadora das transformações social, econômica, política e cultural das comunidades, na promoção do seu desenvolvimento sustentável. A metodologia da pesquisa tomou por base a análise das redes de relações sociais engendradas no processo, elegendo-se como categorias preferências para a compreensão do fenômeno estudado o capital social, o desenvolvimento local, pedagogias alternativas, currículo escolar e as parcerias intersetoriais, através da ruptura com o antagonismo dos conceitos de indivíduo e de sociedade. Trata-se de pesquisa quanti-qualitativa fundamentada numa abordagem de caráter interativo, onde o discurso emerge como espaço de negociação do sentido e da construção dos sujeitos aprendizes, cujos subsídios somados às evidências quantitativas permitiram o aprofundamento da complexidade dos fenômenos, suas contradições e seu relacionamento com o contexto. A análise dos dados permitiu compreender que a Educação Rural nas áreas pesquisadas vive duas situações: uma, veiculada pelo sistema público de ensino que, salvo algumas experiências pontuais, não atende aos interesses dos povos que habitam e trabalham no campo. Outra, exercitada por Organizações Não Governamentais que valorizando o rural como espaço de vida, forma indivíduos com um repertório de saberes, habilidades e valores capaz de mobilizá-los para uma ação transformadora.

Línguas em contato no Oiapoque: as comunidades indígenas karipuna

Esta pesquisa está direcionada à análise da situação sociolingüística de cinco aldeias nas Terras Indígenas Uaçá no município do Oiapoque no Estado do Amapá: Espírito Santo, Taminã, Cutiti, Santa Isabel e Txipidon. Nesse contexto duas línguas estão presentes, quais sejam, língua crioula e língua portuguesa. O objetivo deste trabalho está centrado no reconhecimento da real situação sociolingüística desse espaço de lingüístico-geográfico, vislumbrando a língua materna da etnia, as comunidades, a situação dos sujeitos bilíngües, os usos sociais das línguas e situação escolar em contexto bilíngüe. A análise é embasada na concepção de diglossia sem estabilidade e de bilingüismo como fenômeno não estável, situado, com definição de sujeito bilíngüe relacionado ao contexto em que as línguas se encontram. A situação escolar é vista tendo por base os planejamentos lingüísticos de Hamel. Os dados são analisados sob a ótica da metodologia descritivo-interpretativa, com dados quantitativos auxiliando na descrição qualitativa da pesquisa. Moradores das cinco aldeias e professores índios e não índios fazem parte do corpus da pesquisa. Os resultados confirmam a língua portuguesa como a mais representativa, no repertório verbal das aldeias Santa Isabel e Txipidon e a língua crioula como língua materna de Espírito Santo, Taminã e Cutiti, aldeias de uma etnia em comum, a Karipuna.. Essas comunidades estão em conflito diglóssico, com a língua portuguesa ocupando espaço da língua crioula em alguns domínios lingüísticos. Confirma-se o bilingüismo apresentado pelos falantes como relativo ao contexto, com graus diferenciados de bilinguidade em cada aldeia, considerando-se as quatro habilidades lingüística: compreender, falar, ler e escreve. Essa é a situação que deve ser considerada pela escola em seus planejamentos lingüísticos e não somente as diferentes línguas maternas das comunidades.

O artista plástico e seus públicos na perspectiva da assessoria de relações públicas

This work aims to study and see the importance of building the image and reputation of the artist through a publishing of public relations, public recital, new technologies, media, cultural production and curatorial work. To this, we analyzed the history of the profession of public relations in Brazil. As well as their concepts, practices and definitions. Focus on work of publicist and cultural production sectors. The choice of theme is done by the need to understand the relationships. That intertwines in the art market and what media that best reaches in their audiences. Considering the artist-consumer relationship, and the relationship brand-artist and artist-public. And so, the importance of this advice means less explored public relations, for the creation of the brand concept for the artist before his public. Considered in this work as a gallery, art dealer, curator, media and consumers. In this context an analysis is done on diverse views on the market of the arts through specific interview. This interviews was made with professionals working in the cultural sector. And help to understand the communication segments that exist and the current conditions of the market in the context of competitiveness. As was considered the academic education of the public relations professional, it geared to the cultural context, the interviews with professional future. And then was considered that public relations presents itself as a proposal for better communication in the arts. They are concern to create concepts of brand identity-thinking artist and the best means for the artist to be present in this area