Ultrasonographic, computed tomographic, and operative findings in dogs infested with giant kidney worms (Dioctophyme renale)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Sistema de comunicação bluetooth voltada para automação residencial

Bluetooth technology found in smartphones and tablets can add an interesting feature focused on home automation. Through the implementation of a wireless network communication between a mobile device and receiver modules located in different points, or even on a single centralized point in residence, you can manage many household items with just a few taps on smartphones. With such applicability, allow the user greater integration with your home, comfort, safety and economy. In this context, this work presents a system design of low cost Bluetooth communication between a smartphone and a receiver module with microcontroller interface development control from the Android platform

Aplicativo Android para aquisição de dados de um baropodômetro via Bluetooth para apoio na análise de escoliose

Pós-graduação em Engenharia Elétrica - FEIS

Effectiveness of hyperbaric oxygen for experimental treatment of schistosomiasis mansoni using praziquantel-free and encapsulated into liposomes: Assay in adult worms and oviposition

The treatment of schistosomiasis depends on a single drug: praziquantel (PZQ). However, this treatment presents limitations such as low and/or erratic bioavailability that can contribute to cases of tolerance. Improvements to the available drug are urgently needed and studies with a controlled system of drug release, like liposomes, have been gaining prominence. The present study evaluated the activity and synergy between liposomal-praziquantel (lip.PZQ) and hyperbaric oxygen therapy (HBO). Mice received doses of 60 or 100mg/kg PZQ or lip.PZQ, 50 days post-infection, and after the treatment, were exposed to HBO (3 atmosphere absolute - ATA) for 1h. The viability of adult worms and oviposition were analyzed, by necropsy and Kato-Katz examination performed after 15 days of treatment. A concentration of 100mg/kg of lip.PZQ+HBO was more effective (48.0% reduction of worms, 83.3% reduction of eggs/gram of feces) and 100% of the mice had altered of oograms (indicating interruption of oviposition) compared to other treatments and to the Control group (infected and untreated). It is known that PZQ requires participation of the host immune system to complete its antischistosomal activity and that HBO is able to stimulate the immune system. The drug became more available in the body when incorporated into liposomes and, used with HBO, the HBO worked as an adjuvant. This explains the decreases of oviposition and worms recovered form hepatic portal system.

Controle e aquisição de dados experimentais com tecnologia bluetooth em dispositivos móveis

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Schistosomicidal evaluation of flavonoids from two species of Styrax against Schistosoma mansoni adult worms

Context: Schistosomiasis is a major health problem worldwide. Thus, the search for new schistosomicidal agents from natural sources can provide prototypes for drug discovery. Objective: The present study investigated the chemical composition of the EtOAc fractions of Styrax pohlii Pohl (Styracaceae) (EF-SP) aerial parts and S. camporum A. DC. leaves (EF-SC), as well as schistosomicidal activities against Schistosoma mansoni adult worms, which have not yet been studied. Materials and methods: The crude ethanol extracts of S. camporum leaves and S. pohlii aerial parts (EE-SC and EE-SP) were partitioned with n-hexane, EtOAc, and n-BuOH. The EtOAc fractions were purified by preparative HPLC. The crude extracts, EtOAc fractions and pure compounds were tested against S. mansoni adult worms in vitro. Results: The purification procedure resulted in the isolation of kaempferol-3-O-(2 '',4 ''-di-O-(E)-p-coumaroyl)-beta-D-glucopyranoside (1), kaempferol-3-O-(2 '',6 ''-di-O-(E)-p-coumaroyl)-beta-D-glucopyranoside (2), quercetin (3), and kaempferol (4). The bioassay results indicated that EE-SC, EF-SC, EF-SP, and compounds 2 and 4 are able to separate coupled S. mansoni adult worms. Additionally, EE-SC, EF-SP, and compound 4 killed the adult schistosomes in vitro at 100 mu g/mL and 100 mu M. Discussion and conclusion: This is the first time that the presence of compounds 1-2 in S. pohlii and 3-4 in S. camporum has been reported. Additionally, biological results indicated that S. pohlii and S. camporum have great potential as a source of active compounds.

Effect of human TGF-beta on the gene expression profile of Schistosoma mansoni adult worms

Schistosoma mansoni is responsible for schistosomiasis, a parasitic disease that affects 200 million people worldwide. Molecular mechanisms of host-parasite interaction are complex and involve a crosstalk between host signals and parasite receptors. TGF-beta signaling pathway has been shown to play an important role in S. mansoni development and embryogenesis. In particular human (h) TGF-beta has been shown to bind to a S. mansoni receptor, transduce a signal that regulates the expression of a schistosome target gene. Here we describe 381 parasite genes whose expression levels are affected by in vitro treatment with hTGF-beta. Among these differentially expressed genes we highlight genes related to morphology, development and cell cycle that could be players of cytokine effects on the parasite. We confirm by qPCR the expression changes detected with microarrays for 5 out of 7 selected genes. We also highlight a set of non-coding RNAs transcribed from the same loci of protein-coding genes that are differentially expressed upon hTCF-beta treatment. These datasets offer potential targets to be explored in order to understand the molecular mechanisms behind the possible role of hTGF-beta effects on parasite biology. (C) 2012 Elsevier B.V. All rights reserved.

A quantitative view of the transcriptome of Schistosoma mansoni adult-worms using SAGE

Abstract Background Five species of the genus Schistosoma, a parasitic trematode flatworm, are causative agents of Schistosomiasis, a disease that is endemic in a large number of developing countries, affecting millions of patients around the world. By using SAGE (Serial Analysis of Gene Expression) we describe here the first large-scale quantitative analysis of the Schistosoma mansoni transcriptome, one of the most epidemiologically relevant species of this genus. Results After extracting mRNA from pooled male and female adult-worms, a SAGE library was constructed and sequenced, generating 68,238 tags that covered more than 6,000 genes expressed in this developmental stage. An analysis of the ordered tag-list shows the genes of F10 eggshell protein, pol-polyprotein, HSP86, 14-3-3 and a transcript yet to be identified to be the five top most abundant genes in pooled adult worms. Whereas only 8% of the 100 most abundant tags found in adult worms of S. mansoni could not be assigned to transcripts of this parasite, 46.9% of the total ditags could not be mapped, demonstrating that the 3 sequence of most of the rarest transcripts are still to be identified. Mapping of our SAGE tags to S. mansoni genes suggested the occurrence of alternative-polyadenylation in at least 13 gene transcripts. Most of these events seem to shorten the 3 UTR of the mRNAs, which may have consequences over their stability and regulation. Conclusion SAGE revealed the frequency of expression of the majority of the S. mansoni genes. Transcriptome data suggests that alternative polyadenylation is likely to be used in the control of mRNA stability in this organism. When transcriptome was compared with the proteomic data available, we observed a correlation of about 50%, suggesting that both transcriptional and post-transcriptional regulation are important for determining protein abundance in S. mansoni. The generation of SAGE tags from other life-cycle stages should contribute to reveal the dynamics of gene expression in this important parasite.

Discovery di dispositivi embedded e raccolta di dati con bluetooth e bluetooth smart

L'obbiettivo di questa tesi è la produzione del prototipo di un sistema che sia in grado di ottenere dati da un insieme di sensori per poterli poi trasmettere all’utente, in modo che esso sia maggiormente cosciente del mondo che lo circonda. Affronteremo la sfida in uno scenario medico / di soccorso, dove un operatore si avvicinerà ad un gruppo di pazienti con l’intenzione di ottenere i parametri vitali di uno di essi. All'interno del documento saranno descritte le tecnologie sfruttate per la realizzazione del prototipo: Bluetooth e Bluetooth Smart, il sistema operativo Linux in esecuzione su un Raspberry Pi dotato di sensori, il sistema operativo Android in esecuzione su smartphone o tablet e iBeacon. Verranno poi analizzati i requisiti del sistema da realizzare. Infine verrà descritta l'implementazione utilizzata nel prototipo e analizzato il suo comportamento.

Libreria per comunicazioni ad-hoc per android basate su bluetooth

La tesi sviluppa, attraverso un processo definito e gestito, una libreria Android che permette di far comunicare diversi dispositivi mobili tramite Bluetooth. Inoltre gestisce: • le richieste di connessione sia che esse provengano dall’esterno, sia che partano dal dispositivo stesso; • la dinamicità del sistema utilizzando dispositivi mobili, cioè capaci di muoversi in diverse direzioni costantemente. In tal senso, la lista dei dispositivi vicini è costantemente aggiornata. • la comunicazione in stile Peer-to-Peer(P2P). In questo caso si viene a formare un gap in quanto la libreria Android di Bluetooth usa lo stile Master-Slave per i suoi dispositivi. Per colmare questo gap, la libreria sviluppata crea un layer soprastante la libreria Bluetooth di Android e maschera lo stile di comunicazione di quest’ultima a favo- re di una comunicazione paritaria, nella quale un dispositivo può sia accettare le richieste di connessione, sia connettersi ad altri dispositivi.

Prototipo di schermo adattativo basato su Android e Bluetooth Low Energy

La tesi nasce da un'attività di ricerca approfondita su alcune tecnologie innovative e all'avanguardia nell'ambito del mobile computing e dell'Internet of Things. Tra queste il focus principale è orientato allo studio del Bluetooth Low Energy, nuova specifica di Bluetooth caratterizzata principalmente da un consumo di energia assai ridotto. In particolare, si è approfondito il suo funzionamento nel caso dei beacon, piccoli dispositivi che permettono una localizzazione mediante l'invio di un segnale BLE. Inoltre è stata analizzata la possibilità di interazione con Android, poiché oggigiorno non si può evitare di pensare a come queste tecnologie possano interfacciarsi con il mondo degli smartphone e dei tablet. Come conseguenza di tale attività di ricerca è stato analizzato un caso di studio che permettesse di applicare le tecnologie studiate e la loro interazione. Si è pensato quindi a un piccolo sistema distribuito per schermi adattativi (capaci di modificare i contenuti visualizzati in relazione ad eventi esterni) affinché ogni schermo mostrasse dinamicamente l'orario delle lezioni di ciascuno studente all'avvicinarsi di quest'ultimo, e solo per il tempo di permanenza nei pressi dello schermo. Si è quindi progettato e sviluppato un prototipo, e infine durante il testing si sono potute verificare le attuali potenzialità di queste tecnologie e trarre conclusioni sulla possibilità della loro futura diffusione e di impiego in contesti differenti.