Production of novel ACE inhibitory peptides from β-lactoglobulin using Protease N Amano

Potent angiotensin I-converting enzyme (ACE) inhibitory peptide mixtures were obtained from the hydrolysis of β-lactoglobulin (βLg) using Protease N Amano, a food-grade commercial proteolytic preparation. Hydrolysis experiments were carried out for 8 h at two different temperatures and neutral pH. Based on their ACE inhibitory activity, samples of 6 h of digestion were chosen for further analysis. The temperature used for the hydrolysis had a marked influence on the type of peptides produced and their concentration in the hydrolysate. Protease N Amano was found to produce very complex peptide mixtures; however, the partially fractionated hydrolysates had already very potent ACE inhibitory activity. The novel heptapeptide SAPLRVY was isolated and characterised. It corresponded to βLg f(36–42) and had an IC50 value of 8 μm, which is considerably lower than the most potent ACE inhibitory peptides derived from bovine βLg reported so far.

Enforcing service level agreements using an economically enhanced resource manager

Traditional resource management has had as its main objective the optimisation of throughput, based on parameters such as CPU, memory, and network bandwidth. With the appearance of Grid Markets, new variables that determine economic expenditure, benefit and opportunity must be taken into account. The SORMA project aims to allow resource owners and consumers to exploit market mechanisms to sell and buy resources across the Grid. SORMA’s motivation is to achieve efficient resource utilisation by maximising revenue for resource providers, and minimising the cost of resource consumption within a market environment. An overriding factor in Grid markets is the need to ensure that desired Quality of Service levels meet the expectations of market participants. This paper explains the proposed use of an Economically Enhanced Resource Manager (EERM) for resource provisioning based on economic models. In particular, this paper describes techniques used by the EERM to support revenue maximisation across multiple Service Level Agreements.

Enforcing service level agreements using an economically enhanced resource manager

Traditional resource management has had as its main objective the optimisation of throughput, based on pa- rameters such as CPU, memory, and network bandwidth. With the appearance of Grid Markets, new variables that determine economic expenditure, benefit and opportunity must be taken into account. The SORMA project aims to allow resource owners and consumers to exploit market mechanisms to sell and buy resources across the Grid. SORMA’s motivation is to achieve efficient resource utilisation by maximising revenue for resource providers, and minimising the cost of resource consumption within a market environment. An overriding factor in Grid markets is the need to ensure that desired Quality of Service levels meet the expectations of market participants. This paper explains the proposed use of an Economically Enhanced Resource Manager (EERM) for resource provisioning based on economic models. In particular, this paper describes techniques used by the EERM to support revenue maximisation across multiple Service Level Agreements.

Methane cross-validation between three Fourier Transform Spectrometers: SCISAT ACE-FTS, GOSAT TANSO-FTS, and ground-based FTS measurements in the Canadian high Arctic

We present cross-validation of remote sensing measurements of methane profiles in the Canadian high Arctic. Accurate and precise measurements of methane are essential to understand quantitatively its role in the climate system and in global change. Here, we show a cross-validation between three datasets: two from spaceborne instruments and one from a ground-based instrument. All are Fourier Transform Spectrometers (FTSs). We consider the Canadian SCISAT Atmospheric Chemistry Experiment (ACE)-FTS, a solar occultation infrared spectrometer operating since 2004, and the thermal infrared band of the Japanese Greenhouse Gases Observing Satellite (GOSAT) Thermal And Near infrared Sensor for carbon Observation (TANSO)-FTS, a nadir/off-nadir scanning FTS instrument operating at solar and terrestrial infrared wavelengths, since 2009. The ground-based instrument is a Bruker 125HR Fourier Transform Infrared (FTIR) spectrometer, measuring mid-infrared solar absorption spectra at the Polar Environment Atmospheric Research Laboratory (PEARL) Ridge Lab at Eureka, Nunavut (80° N, 86° W) since 2006. For each pair of instruments, measurements are collocated within 500 km and 24 h. An additional criterion based on potential vorticity values was found not to significantly affect differences between measurements. Profiles are regridded to a common vertical grid for each comparison set. To account for differing vertical resolutions, ACE-FTS measurements are smoothed to the resolution of either PEARL-FTS or TANSO-FTS, and PEARL-FTS measurements are smoothed to the TANSO-FTS resolution. Differences for each pair are examined in terms of profile and partial columns. During the period considered, the number of collocations for each pair is large enough to obtain a good sample size (from several hundred to tens of thousands depending on pair and configuration). Considering full profiles, the degrees of freedom for signal (DOFS) are between 0.2 and 0.7 for TANSO-FTS and between 1.5 and 3 for PEARL-FTS, while ACE-FTS has considerably more information (roughly 1° of freedom per altitude level). We take partial columns between roughly 5 and 30 km for the ACE-FTS–PEARL-FTS comparison, and between 5 and 10 km for the other pairs. The DOFS for the partial columns are between 1.2 and 2 for PEARL-FTS collocated with ACE-FTS, between 0.1 and 0.5 for PEARL-FTS collocated with TANSO-FTS or for TANSO-FTS collocated with either other instrument, while ACE-FTS has much higher information content. For all pairs, the partial column differences are within ± 3 × 1022 molecules cm−2. Expressed as median ± median absolute deviation (expressed in absolute or relative terms), these differences are 0.11 ± 9.60 × 10^20 molecules cm−2 (0.012 ± 1.018 %) for TANSO-FTS–PEARL-FTS, −2.6 ± 2.6 × 10^21 molecules cm−2 (−1.6 ± 1.6 %) for ACE-FTS–PEARL-FTS, and 7.4 ± 6.0 × 10^20 molecules cm−2 (0.78 ± 0.64 %) for TANSO-FTS–ACE-FTS. The differences for ACE-FTS–PEARL-FTS and TANSO-FTS–PEARL-FTS partial columns decrease significantly as a function of PEARL partial columns, whereas the range of partial column values for TANSO-FTS–ACE-FTS collocations is too small to draw any conclusion on its dependence on ACE-FTS partial columns.

Climatology and variability of trace gases in extratropical double-tropopause regions from MLS, HIRDLS, and ACE-FTS measurements

Upper tropospheric and lower stratospheric measurements from the Aura Microwave Limb Sounder (MLS), the Aura High Resolution Dynamics Limb Sounder (HIRDLS), and the Atmospheric Chemistry Experiment-Fourier transform spectrometer (ACE-FTS) are used to present the first global climatological comparison of extratropical, nonpolar trace gas distributions in double-tropopause (DT) and single-tropopause (ST) regions. Stratospheric tracers, O3, HNO3, and HCl, have lower mixing ratios ∼2–8 km above the primary (lowermost) tropopause in DT than in ST regions in all seasons, with maximum Northern Hemisphere (NH) differences near 50% in winter and 30% in summer. Southern Hemisphere winter differences are somewhat smaller, but summer differences are similar in the two hemispheres. H2O in DT regions of both hemispheres shows strong negative anomalies in November through February and positive anomalies in July through October, reflecting the strong seasonal cycle in H2O near the tropical tropopause. CO and other tropospheric tracers examined have higher DT than ST values 2–7 km above the primary tropopause, with the largest differences in winter. Large DT-ST differences extend to high NH latitudes in fall and winter, with longitudinal maxima in regions associated with enhanced wave activity and subtropical jet variations. Results for O3 and HNO3 agree closely between MLS and HIRDLS, and differences from ACE-FTS are consistent with its sparse and irregular midlatitude sampling. Consistent signatures in climatological trace gas fields provide strong evidence that transport from the tropical upper troposphere into the layer between double tropopauses is an important pathway for stratosphere-troposphere exchange.

Ace gene dosage influences the development of renovascular hypertension

P>1. Clinical and experimental evidence highlights the importance of the renin-angiotensin system in renovascular hypertension. Furthermore, genetic factors affecting angiotensin-converting enzyme (ACE) could influence the development of renovascular hypertension. 2. To test the effect of small gene perturbations on the development of renovascular hypertension, mice harbouring two or three copies of the Ace gene were submitted to 4 weeks of two-kidney, one-clip (2K1C) hypertension. Blood pressure (BP), cardiac hypertrophy, baroreflex sensitivity and blood pressure and heart rate variability were assessed and compared between the different groups. 3. The increase in BP induced by 2K1C was higher in mice with three copies of the Ace gene compared with mice with only two copies (46 vs 23 mmHg, respectively). Moreover, there was a 3.8-fold increase in the slope of the left ventricle mass/BP relationship in mice with three copies of the Ace gene. Micewith three copies of the Ace gene exhibited greater increases in cardiac and serum ACE activity than mice with only two copies of the gene. Both baroreflex bradycardia and tachycardia were significantly depressed in mice with three copies of the Ace gene after induction of 2K1C hypertension. The variance in basal systolic BP was greater in mice with three copies of the Ace gene after 2K1C hypertension compared with those with only two copies of the gene (106 vs 54%, respectively). In addition, the low-frequency component of the pulse interval was higher mice with three copies of the Ace gene after 2K1C hypertension compared with those with only two (168 vs 86%, respectively). Finally, in mice with three copies of the Ace gene, renovascular hypertension induced a 6.1-fold increase in the sympathovagal balance compared with a 3.2-fold increase in mice with only two copies of the gene. 4. Collectively, these data provide direct evidence that small genetic disturbances in ACE levels per se have an influence on haemodynamic, cardiac mass and autonomic nervous system responses in mice under pathological perturbation.

ACE inhibition by astilbin isolated from Erythroxylum gonocladum (Mart.) OE Schulz

Erythroxylum species have several traditional uses in different countries, including the treatment of hypertension. The ethanol extract from E. gonocladum aerial parts, a species endemic to the Brazilian cerrado, elicited a concentration-dependent inhibition of angiotensin converting enzyme (ACE) (pIC(50)=4.53 +/- 0.05). Extract fractionation led to the isolation of two compounds, whose structures were assigned by spectrometric data as astilbin and beta-sitosterol, along with a mixture of palmitic, stearic and linolenic acids. This is the first report on the occurrence of these compounds on E. gonocladum. Astilbin promoted significant ACE inhibition in vitro (pIC(50)=5.86 +/- 0.33) and its activity did not differ from captopril, when both compounds were assayed at 10 mu M concentration. (C) 2009 Elsevier GmbH. All rights reserved.

Manager's task to support integrated autonomy at the workplace : Results from an intervention

A new managerial task arises in today’s working life: to provide conditions for and influence interaction between actors and thus to enable the emergence of organizing structure in tune with a changing environment. We call this the enabling managerial task. The goal of this paper is to study whether training first line managers in the enabling managerial task could lead to changes in the work for the subordinates. This paper presents results from questionnaires answered by the subordinates of the managers before and after the training. The training was organized as a learning network and consisted of eight workshops carried out over a period of one year (September 2009–June 2010), where the managers met with each other and the researchers once a month. Each workshop consisted of three parts, during three and a half hours. The first hour was devoted to joint reflection on a task that had been undertaken since the last workshop; some results were presented from the employee pre-assessments, followed by relevant theory and illuminating practices, finally the managers created new tasks for themselves to undertake during the following month. The subordinates’ answers show positive change in all of the seventeen scales used to assess it. The improvements are significant in scales measuring the relationship between the manager and the employees, as well as in those measuring interaction between employees. It is concluded that the result was a success for all managers that had the possibility of using the training in their management work.

The Profile of a Manager in Higher Education Institution in Latin America: The Case of the EBAPE

The aim of this study is to analyze the profile of today’s manager, according to the classical management literature, working on internationalized higher education institute. This paper draws together knowledge about the manager’s profile in a corporate environment and how it can be appreciated in a higher education institute all of this taking into consideration the environment that nowadays exists in these institutions. Data were collected through semi-structured interviews, with professors of the Fundacao Getulio Vargas, more specific on their Public and Business Administration School (EBAPE), which had active roles on its development, internationalization and during some point in time their management. The findings obtained where analyzed under the perspective brought by the theoretical reference framework, and, based on this approach is represented the manager whom could have a better perspective to administrate a higher education institute.

WISE-MANager: criação e gestão de sessões colaborativas em redes de sensores sem fio

As redes de sensores sem fios (WSN- WSN-Wireless Sensor Network) utilizam um grande número de dispositivos sem fios (sensores), que são de baixo custo e equipados com interfaces wireless. Utilizam um conjunto de sensores autónomos que colaboram entre si para efectuar a monitorização das condições ambientais, tais como: temperatura, oxigénio, luz, humidade, pressão, gases poluentes, entre outras. Estas redes podem operar durante largos períodos de tempo, sem intervenção humana, sendo que esse tempo depende do nível de bateria desses nós. De modo a que os gestores de um Museu possam gerir de forma mais adequada as obras de arte e arquivos históricos, surge o projecto WISE-MUSE – Environmental Monitoring based on Wireless Sensor Networks, que permite implementar soluções para a monitorização museológica, com a utilização de redes de sensores sem fios. Actualmente, a colaboração entre o utilizador e a WSN é muito ténue, sendo que apenas existe colaboração entre os nós sensores. De forma a aumentar esta colaboração, e no âmbito do projecto WISE-MUSE surge o CWSN – Collaborative Wireless Sensor Network Model, que define um modelo de colaboração na rede de sensores sem fios, permitindo a utilização de sessões colaborativas para a monitorização da rede. Com o intuito de obter o máximo rendimento da rede, é necessário definir qual o deployment a utilizar. O tipo de deployment de uma WSN é a forma como os nós são distribuídos pela rede. Em zonas longínquas, ou de difícil acesso, os nós são colocados de forma aleatória, por exemplo, caiem de um avião. Nos locais de fácil acesso, podem ser colocados no local exacto. Portanto, este projecto de Mestrado de Engenharia Informática apresenta duas contribuições principais: (i) um estudo de propagação no Museu da Baleia; e; (ii) o WISE-MANager, um sistema de gestão de sessões colaborativas. De forma a definir qual o deployment da rede a instalar no Museu da Baleia, será apresentado um estudo de propagação do sinal empírico, que determinou a melhor posição física dos nós, para que a rede tenha uma boa performance. O sistema WISE-MANager permite a criação, monitorização e gestão de sessões colaborativas numa WSN baseada no protocolo Zigbee. O intuito da utilização de sessões colaborativas é proporcionar uma melhor interacção entre o utilizador e a WSN, visto que o utilizador pode personalizar o tipo de monitorização a efectuar (por nó sensor, por fenómeno ou por intervalo de tempo), e interrogar à rede e aos seus componentes, aumentando assim a flexibilidade da WSN.A gestão de redes de sensores sem fios é muito importante para que o utilizador tenha controlo sobre a mesma ao saber quais os dispositivos da rede, assim como o seu nível de energia. Por tanto, através de WISE-MANager, os gestores do Museu serão capazes de analisar a rede, detectar eventuais problemas e obter parâmetros específicos.

Un acercamiento del "Titulado Arquitecto" en España, al ejercicio profesional del project manager en Europa

Programa de doctorado: Restauración y rehabilitación arquitetónica. Investigación, tendencias e innovaciones. La fecha de publicación es la fecha de lectura

Studio e sviluppo di un'applicazione mobile per iOS nell'ambito della BI: un task manager per SAP Business Planning and Consolidation

La tesi riguarda lo sviluppo di un'applicazione che estende la possibilità di effettuare i caricamenti dei package di SAP BPC ai dispositivi mobile, fino ad ora questo era possibile solo attraverso l'interfaccia di Microsoft Excel.

Workout Manager Android App

L’obiettivo di questa tesi è quello di progettare e sviluppare un'applicazione Android inerente alle palestre. In particolare l'utente finale dovrà essere in grado di poter scaricare i propri allenamenti da un server SQL remoto e visualizzarli con un layout grafico che ne ricordi la struttura di un allenamento cartaceo (con l’utilizzo quindi di schede, esercizi, serie, ripetizioni,...). Attraverso una procedura guidata, l’applicazione dovrà seguire l’utente durante il suo allenamento e registrarne i progressi. Questi ultimi dovranno essere memorizzati permanentemente e visualizzati attraverso dei grafici.

Human Presence Detection: analisi comparativa e caso di studio in Home Manager

La possibilità di monitorare l’attività degli utenti in un sistema domotico, sia considerando le azioni effettuate direttamente sul sistema che le informazioni ricavabili da strumenti esterni come la loro posizione GPS, è un fattore importante per anticipare i bisogni e comprendere le preferenze degli utenti stessi, rendendo sempre più intelligenti ed autonomi i sistemi domotici. Mentre i sistemi attualmente disponibili non includono o non sfruttano appieno queste potenzialità, l'obiettivo di sistemi prototipali sviluppati per fini di ricerca, quali ad esempio Home Manager, è invece quello di utilizzare le informazioni ricavabili dai dispositivi e dal loro utilizzo per abilitare ragionamenti e politiche di ordine superiore. Gli obiettivi di questo lavoro sono: - Classificare ed elencare i diversi sensori disponibili al fine di presentare lo stato attuale della ricerca nel campo dello Human Sensing, ovvero del rilevamento di persone in un ambiente. - Giustificare la scelta della telecamera come sensore per il rilevamento di persone in un ambiente domestico, riportando metodi per l’analisi video in grado di interpretare i fotogrammi e rilevare eventuali figure in movimento al loro interno. - Presentare un’architettura generica per integrare dei sensori in un sistema di sorveglianza, implementando tale architettura ed alcuni algoritmi per l’analisi video all’interno di Home Manager con l’aiuto della libreria OpenCV .