Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins.

Advanced glycation endproducts (AGEs) are derivatives of nonenzymatic reactions between sugars and protein or lipids, and together with AGE-specific receptors are involved in numerous pathogenic processes associated with aging and hyperglycemia. Two of the known AGE-binding proteins isolated from rat liver membranes, p60 and p90, have been partially sequenced. We now report that the N-terminal sequence of p60 exhibits 95% identity to OST-48, a 48-kDa member of the oligosaccharyltransferase complex found in microsomal membranes, while sequence analysis of p90 revealed 73% and 85% identity to the N-terminal and internal sequences, respectively, of human 80K-H, a 80- to 87-kDa protein substrate for protein kinase C. AGE-ligand and Western analyses of purified oligosaccharyltransferase complex, enriched rough endoplasmic reticulum, smooth endoplasmic reticulum, and plasma membranes from rat liver or RAW 264.7 macrophages yielded a single protein of approximately 50 kDa recognized by both anti-p60 and anti-OST-48 antibodies, and also exhibited AGE-specific binding. Immunoprecipitated OST-48 from rat rough endoplasmic reticulum fractions exhibited both AGE binding and immunoreactivity to an anti-p60 antibody. Immune IgG raised to recombinant OST-48 and 80K-H inhibited binding of AGE-bovine serum albumin to cell membranes in a dose-dependent manner. Immunostaining and flow cytometry demonstrated the surface expression of OST-48 and 80K-H on numerous cell types and tissues, including mononuclear, endothelial, renal, and brain neuronal and glial cells. We conclude that the AGE receptor components p60 and p90 are identical to OST-48, and 80K-H, respectively, and that they together contribute to the processing of AGEs from extra- and intracellular compartments and in the cellular responses associated with these pathogenic substances.

Abrir a história : a imagem de arquivo e o movimento desacelarado : um estudo teórico-prático a partir dos filmes Natureza Morta e 48

Tese de doutoramento, Belas-Artes (Audiovisuais), Universidade de Lisboa, Faculdade de Belas-Artes, 2014

Unesp Ciência, 2013, ano 5, número 48

Revista elaborada pela Assessoria de Comunicação e Imprensa da Reitoria da UNESP

Maneio do cão geriátrico nas 48 horas pós-cirúrgicas

Dissertação de Mestrado Integrado em Medicina Veterinária

Entre ciel et terre : aventures d'un détective aviateur / J. Jacquin et A. Fabre ; ouvrage illustré de 48 gravures par G. Dutriac

Collection : Bibliothèque des écoles et des familles

Archives parlementaires de 1787 à 1860 ; 34-51. Assemblée nationale législative. Série 1 / Tome 48 / impr. par ordre du Sénat et de la Chambre des députés ; sous la dir. de M. J. Mavidal,... et de M. E. Laurent,... ; avec la collab. de MM. E. Tonnier et C. Pionnier

Collection : Les archives de la Révolution française ; 3.1