Dinâmica das configurações de formação e inibição das chuvas no Rio Grande do Norte: caracterização hidroclimática do estado

This study aimed to evaluate the influence of the main meteorological mechanisms trainers and inhibitors of precipitation, and the interactions between different scales of operation, the spatial and temporal variability of the annual cycle of precipitation in the Rio Grande do Norte. Além disso, considerando as circunstâncias locais e regionais, criando assim uma base científica para apoiar ações futuras na gestão da demanda de água no Estado. Database from monthly precipitation of 45 years, ranging between 1963 and 2007, data provided by EMPARN. The methodology used to achieve the results was initially composed of descriptive statistical analysis of historical data to prove the stability of the series, were applied after, geostatistics tool for plotting maps of the variables, within the geostatistical we opted for by Kriging interpolation method because it was the method that showed the best results and minor errors. Among the results, we highlight the annual cycle of rainfall the State which is influenced by meteorological mechanisms of different spatial and temporal scales, where the main mechanisms cycle modulators are the Conference Intertropical Zone (ITCZ) acting since midFebruary to mid May throughout the state, waves Leste (OL), Lines of instability (LI), breeze systems and orographic rainfall acting mainly in the Coastal strip between February and July. Along with vortice of high levels (VCANs), Complex Mesoscale Convective (CCMs) and orographic rain in any region of the state mainly in spring and summer. In terms of larger scale phenomena stood out El Niño and La Niña, ENSO in the tropical Pacific basin. In La Niña episodes usually occur normal or rainy years, as upon the occurrence of prolonged periods of drought are influenced by EL NIÑO. In the Atlantic Ocean the standard Dipole also affects the intensity of the rainfall cycle in State. The cycle of rains in Rio Grande do Norte is divided into two periods, one comprising the regions West, Central and the Western Portion of the Wasteland Potiguar mesoregions of west Chapada Borborema, causing rains from midFebruary to mid-May and a second period of cycle, between February-July, where rains occur in mesoregions East and of the Wasteland, located upwind of the Chapada Borborema, both interspersed with dry periods without occurrence of significant rainfall and transition periods of rainy - dry and dry-rainy where isolated rainfall occur. Approximately 82% of the rainfall stations of the state which corresponds to 83.4% of the total area of Rio Grande do Norte, do not record annual volumes above 900 mm. Because the water supply of the State be maintained by small reservoirs already are in an advanced state of eutrophication, when the rains occur, act to wash and replace the water in the reservoirs, improving the quality of these, reducing the eutrophication process. When rain they do not significantly occur or after long periods of shortages, the process of eutrophication and deterioration of water in dams increased significantly. Through knowledge of the behavior of the annual cycle of rainfall can have an intimate knowledge of how it may be the tendency of rainy or prone to shortages following period, mainly observing the trends of larger scale phenomena

Ações de cuidado nas relações intrageracionais em crianças em acolhimento institucional

According to the Statute of Children and Adolescents (1990) children and adolescents are conceived as subjects of rights, with absolute priority and development peculiar condition. Thus, if these rights were violated or threatened, will be applied protection measures. Within these measures, in that Statute, the foster institutional is proposed, with transitional and exceptional character. When the child goes out from family and community life, and she is upheld in an institution, the child is placed in a new development context, therefore, with new people, new places, and new relationships. According with Socio-Historical Psychology, theoretical support of this study, each context presents specific demands of socialization that influence child development and her subjectivity is constituted through the relations that the subject establishes in each context. These contexts bring challenges and proposals for the child and she needs to respond these. Then, whereas he is in relation to the other, in this moment, the subject is constituted, the interactions established during the foster institutional will be of paramount importance to the child. Among these interactions, we can cite situations involving aspects of moral development, specifically those that can ask (or not) the exercise of the virtues. About the intersection between these actions can then arise care actions beyond those involving the attending of an emerging need. The objective of this study is to investigate the presence of relation everyday permeated by care actions among children in foster institutional. For the scope of the objective three children were participated, with three years old and in foster care measure. The research is qualitative and the procedure for building the corpus was, mainly, the participant observation. Procedures with video and history in books were also used as supplementary procedures. The analysis of the corpus was made through Thematic Content Analysis, the episodes were grouped into analysis categories pre-and post-established. The preestablished were care actions related to body care, care actions related to socio affective aspects, and care actions related to body care and socio affective aspects simultaneously. The two post-established categories were dismemberment of the preceding categories, called care actions developed in child-child interaction, without the intervention of an adult, and care actions developed in child-child interaction, with direct intervention of the educator. The analysis indicated that in the everyday interaction between foster children, they identify the physical and emotional needs of each other foster member, and they are willing to help them in whatever way they can, emphasizing the importance of play and playful moments like mediators about these interactions. The care actions observed are based on children´s concepts and interpretations made from their experiences and largely refer to maternal care. The condition of being away from their family life can be an element that enables these actions. Finally, this study reaffirms the importance of designing the foster institution as a socialization and care space. It follows the importance of valuing and strengthening the positive aspects that arise in the relationships established by the children in this context, including the care actions, the research objective, which are components of the subjectivity of these children

A geometria do compasso (1797) de Mascheroni (1750 – 1800) em atividades com o GeoGebra

The present work aims to show a possible relationship between the use of the History of Mathematics and Information and Communication Technologies (TIC) in teaching Mathematics through activities that use geometric constructions of the “Geometry of the Compass” (1797) by Lorenzo Mascheroni (1750-1800). For this, it was performed a qualitative research characterized by an historical exploration of bibliographical character followed by an empirical intervention based on use of the History of Mathematics combined with TIC through Mathematical Investigation. Thus, studies were performed in papers dealing with the topic, as well as a survey to highlight problems and /or episodes of the history of mathematics that can be solved with the help of TIC, allowing the production of a notebook of activities addressing the resolution of historical problems in a computer environment. In this search, we came across the problems of geometry that are presented by Mascheroni stated previously in the work that we propose solutions and investigations using GeoGebra software. The research resulted in the elaboration of an educational product, a notebook of activities, which was structure to allow during its implementation, students can conduct historical and/or Mathematics research, therefore, we present the procedures for realization of each construction, followed at some moments by original solution of the work. At the same time, we encourage students to investigate/reflect its construction (GeoGebra), in addition to making comparisons with the solution Mascheroni. This notebook was applied to two classes of the course of Didactics of Mathematics I (MAT0367) Course in Mathematics UFRN in 2014. Knowing the existence of some unfavorable arguments regarding the use of history of mathematics, such as loss of time, it was found that this factor can be mitigated with the aid of computational resource, because we can make checks using only the dynamism of and software without repeating the construction. It is noteworthy that the minimized time does not mean loss of reflection or maturation of ideas, when we adopted the process of historical and/or Mathematics Investigation

Padronização de testes para avaliação do estado de mania e potencial anti-maníaco de um agonista do receptor NOP

Bipolar disorder is characterized by mood impairment, alternating between mania/hypomania and depression, and its exact pathophysiology is already unknown. The treatment of bipolar disorder is based on prevention of the manic and depressive episodes using mood stabilizers. Nociceptin/orfanin FQ (N/OFQ) is an endogenous heptadecapeptide which binds as an agonist to NOP receptor, which is a G-coupled inhibitory receptor. N/OFQ and its receptor modulate a lot of functions in the organism, including emotional processes. It is known that the plasmatic concentration of N/OFQ is altered in patients in both phases depressive and manic of bipolar disorder and it is assumed that this system has a role on the etiology of this disorder. Concerning mania, the animal models used in research tend to focus in an unique aspect of the manic behavior, as hyperactivity or agressivity. In the 60’s, the hole board test was proposed, and it consists of an apparatus with holes where a behavior known as head-dippings is measured. High levels of head-dippings are suggestive of neophilia, while low levels can be characteristic of an anxious-like behavior. As the increase of exploratory and goal-directed behavior are characteristics of manic behavior, this test could help in mania research. Thus, this work was organized in 3 steps and aims to: (1) investigate the induction of a manic-like state promoted by ouabain, a Na+/K+-ATPase inhibitor, in the mouse open field test; (2) set up the hole board as a test to measure manic-like behaviors; and (3) investigate the N/OFQ effects in prevention of this kind of behavior on hole board. Male Swiss mice were used in this study, and they take part of only one of the described steps. Depending on the step performed, mice received one or more of the following treatments: (1) ouabain 10-6 , 10-5 , 10-4 , 10-3 or 10-2 M, intracerebroventricular (icv); (2) sodium valproate 300 mg/kg, intraperitoneal (ip); (3) sodium valproate 400 mg/kg, ip; (4) diazepam 1 mg/kg, ip; (5) methylphenidate 10 mg/kg, ip; and (6) N/OFQ 0,1 or 1 nmol, icv. The results suggest that hole board can be used to evaluate a manic state, through analysis of different animal behaviors. However, it was not possible to standard the model of Na+ /K+ -ATPase dysfunction through ouabain administration in mice. Moreover, the data suggest that N/OFQ, at the doses tested, has not affected the methylphenidate-induced mania-like behavior. Taken together, the results point to a new approach of manic research, through the hole board using. However, more studies are necessary in order to verify the role of N/OFQ system on bipolar disorder.

Estudo da estrutura subsuperficial da província Borborema com correlação de ruído sísmico

Ambient seismic noise has traditionally been considered as an unwanted perturbation in seismic data acquisition that "contaminates" the clean recording of earthquakes. Over the last decade, however, it has been demonstrated that consistent information about the subsurface structure can be extracted from cross-correlation of ambient seismic noise. In this context, the rules are reversed: the ambient seismic noise becomes the desired seismic signal, while earthquakes become the unwanted perturbation that needs to be removed. At periods lower than 30 s, the spectrum of ambient seismic noise is dominated by microseism, which originates from distant atmospheric perturbations over the oceans. The microsseism is the most continuous seismic signal and can be classified as primary – when observed in the range 10-20 s – and secondary – when observed in the range 5-10 s. The Green‘s function of the propagating medium between two receivers (seismic stations) can be reconstructed by cross-correlating seismic noise simultaneously recorded at the receivers. The reconstruction of the Green‘s function is generally proportional to the surface-wave portion of the seismic wavefield, as microsseismic energy travels mostly as surface-waves. In this work, 194 Green‘s functions obtained from stacking of one month of daily cross-correlations of ambient seismic noise recorded in the vertical component of several pairs of broadband seismic stations in Northeast Brazil are presented. The daily cross-correlations were stacked using a timefrequency, phase-weighted scheme that enhances weak coherent signals by reducing incoherent noise. The cross-correlations show that, as expected, the emerged signal is dominated by Rayleigh waves, with dispersion velocities being reliably measured for periods ranging between 5 and 20 s. Both permanent stations from a monitoring seismic network and temporary stations from past passive experiments in the region are considered, resulting in a combined network of 33 stations separated by distances between 60 and 1311 km, approximately. The Rayleigh-wave, dispersion velocity measurements are then used to develop tomographic images of group velocity variation for the Borborema Province of Northeast Brazil. The tomographic maps allow to satisfactorily map buried structural features in the region. At short periods (~5 s) the images reflect shallow crustal structure, clearly delineating intra-continental and marginal sedimentary basins, as well as portions of important shear zones traversing the Borborema Province. At longer periods (10 – 20 s) the images are sensitive to deeper structure in the upper crust, and most of the shallower anomalies fade away. Interestingly, some of them do persist. The deep anomalies do not correlate with either the location of Cenozoic volcanism and uplift - which marked the evolution of the Borborema Province in the Cenozoic - or available maps of surface heat-flow, and the origin of the deep anomalies remains enigmatic.

Variabilidade genética de Leishmania spp. circulantes entre seres humanos e cães e infecção de flebotomíneos em áreas endêmicas para as leishmanioses no Rio Grande do Norte

Leishmania infantum is the main etiologic agent of visceral leishmaniasis in the New World. The pattern of distribution of leishmaniasis has changed substantially and has presented an emerging profile within the periphery of the Large Urban Centers. Leishmania infection can compromise skin, mucosa and viscera. Only 10% of the individuals infected develop the disease and 90% of human infection is asymptomatic. The main factors involved in the development of the disease are the host immune response, the vector’s species and the parasite’s genetic content. The sequencing of Leishmania isolated seeks to increase the understanding of the symptoms of individuals. The aim of this study was to evaluate the genetic diversity of circulating Leishmania strains among humans, and symptomatic and asymptomatic, and dogs from endemic areas of Rio Grande do Norte State and analyze sandflies from endemic areas for cutaneous and visceral disease. The genetic variability was evaluated by the use of markers hsp70 , ITS1 and a whole genome sequencing was also carried out. The amplified hsp70 and ITS1 of samples were analyzed and assembled using a Phred / Phrap package. The dendograms were constructed using the same methodology, but adding 500 bootstraps, followed by inferences on the relationships between Leishmania variants. The sequences of the 20 Brazilian isolates were mapped to the reference genome L. infantum JPCM5, using the Bowtie2 program and the identification of 36 contigs. The information of the valid SNPs were used in the PCA. SNPs were visualized by Geneious 7.1 and IGV. The genome annotations were transferred to their respective chromosomes and displayed on Geneious. The matching sequences of all chromosomes were aligned using Mauve. The phylogenetic trees were calculated according to maximum likelihood and JTT models. Sandflies were analyzed by PCR for the identification of Leishmania infection, a blood meal source and GAPDH sand fly. As a result, hsp70 and ITS1 were not capable of identifying genetic variability among human isolates from symptomatic and asymptomatic, and dogs. The complete sequencing of the 20 Brazilian isolates revealed a strong similarity between the circulating Leishmania strains in Rio Grande do Norte. The isolates collected in the city of Natal from humans and canines remained grouped in all analyzes, suggesting that there is genotypic and geographic proximity among the isolates. The isolated samples in the 1990s had a higher genotypic diversity when compared to freshly isolated samples. All isolates presented 36 chromosomes with variable ploidy among them, no correlation was found between the number of amastina genes copies, gp63, A2 and SSG with such clinic forms. In general, we did not find correlation between symptomatic and asymptomatic clinical forms and the gene content of the Brazilian isolates of Leishmania. 34,28% of the sandflies collected in the upper west region were L. longipalpis and the main sources of blood meal were humans, dogs and chickens.

Variabilidade genética de Leishmania spp. circulantes entre seres humanos e cães e infecção de flebotomíneos em áreas endêmicas para as leishmanioses no Rio Grande do Norte

Leishmania infantum is the main etiologic agent of visceral leishmaniasis in the New World. The pattern of distribution of leishmaniasis has changed substantially and has presented an emerging profile within the periphery of the Large Urban Centers. Leishmania infection can compromise skin, mucosa and viscera. Only 10% of the individuals infected develop the disease and 90% of human infection is asymptomatic. The main factors involved in the development of the disease are the host immune response, the vector’s species and the parasite’s genetic content. The sequencing of Leishmania isolated seeks to increase the understanding of the symptoms of individuals. The aim of this study was to evaluate the genetic diversity of circulating Leishmania strains among humans, and symptomatic and asymptomatic, and dogs from endemic areas of Rio Grande do Norte State and analyze sandflies from endemic areas for cutaneous and visceral disease. The genetic variability was evaluated by the use of markers hsp70 , ITS1 and a whole genome sequencing was also carried out. The amplified hsp70 and ITS1 of samples were analyzed and assembled using a Phred / Phrap package. The dendograms were constructed using the same methodology, but adding 500 bootstraps, followed by inferences on the relationships between Leishmania variants. The sequences of the 20 Brazilian isolates were mapped to the reference genome L. infantum JPCM5, using the Bowtie2 program and the identification of 36 contigs. The information of the valid SNPs were used in the PCA. SNPs were visualized by Geneious 7.1 and IGV. The genome annotations were transferred to their respective chromosomes and displayed on Geneious. The matching sequences of all chromosomes were aligned using Mauve. The phylogenetic trees were calculated according to maximum likelihood and JTT models. Sandflies were analyzed by PCR for the identification of Leishmania infection, a blood meal source and GAPDH sand fly. As a result, hsp70 and ITS1 were not capable of identifying genetic variability among human isolates from symptomatic and asymptomatic, and dogs. The complete sequencing of the 20 Brazilian isolates revealed a strong similarity between the circulating Leishmania strains in Rio Grande do Norte. The isolates collected in the city of Natal from humans and canines remained grouped in all analyzes, suggesting that there is genotypic and geographic proximity among the isolates. The isolated samples in the 1990s had a higher genotypic diversity when compared to freshly isolated samples. All isolates presented 36 chromosomes with variable ploidy among them, no correlation was found between the number of amastina genes copies, gp63, A2 and SSG with such clinic forms. In general, we did not find correlation between symptomatic and asymptomatic clinical forms and the gene content of the Brazilian isolates of Leishmania. 34,28% of the sandflies collected in the upper west region were L. longipalpis and the main sources of blood meal were humans, dogs and chickens.

Infecções por enterobacteriaceae resistente aos carbapenêmicos em hospital de ensino: epidemiologia e caracterização molecular

Introduction: The production of KPC (Klebsiella pneumoniae carbapenemase) has become an important mechanism of carbapenem-resistance among Enterobacteriaceae strains. In Brazil, KPC is already widespread and its incidence has increased significantly, reducing treatment options. The “perfect storm” combination of the absence of new drug developmentand the emergence of multidrug-resistant strains resulted in the need for the use of older drugs, with greater toxicity, such as polymyxins. Aims: To determine the occurrence of carbapenemase-producing strains in carbapenem-resistant Enterobacteriaceae isolated from patients with nosocomial infection/colonization during September/2014 to August/2015, to determine the risk factors associated with 30-day- mortality and the impact of inappropriate therapy. Materials and Methods: We performed a case control study to assess the risk factors (comorbidities, invasive procedures and inappropriate antimicrobial therapy) associated with 30-day-mortality, considering the first episode of infection in 111 patients. The resistance genes blaKPC, blaIMP, blaVIM and blaNDM-1 were detected by polymerase chain reaction technique. Molecular typing of the strains involved in the outbreak was performed by pulsed field gel electrophoresis technique. The polymyxin resistance was confirmed by the microdilution broth method. Results: 188 episodes of carbapenem-resistant Enterobacteriaceae infections/colonizations were detected; of these, 122 strains were recovered from the hospital laboratory. The presence of blaKPC gene were confirmed in the majority (74.59%) of these isolates. It was not found the presence of blaIMP , blaVIM and blaNDM-1 genes. K. pneumoniae was the most frequent microorganism (77,13%), primarily responsible for urinary tract infections (21,38%) and infections from patients of the Intensive Care Unit (ICU) (61,38%). Multivariate statistical analysis showed as predictors independently associated with mortality: dialysis and bloodstream infection. The Kaplan-Meier curve showed a lower probability of survival in the group of patients receiving antibiotic therapy inappropriately. Antimicrobial use in adult ICU varied during the study period, but positive correlation between increased incidence of strains and the consumption was not observed. In May and July 2015, the occurrence rates of carbapenem-resistant Enterobacteriaceae KPC-producing per 1000 patient-days were higher than the control limit established, confirming two outbreaks, the first caused by colistin-susceptible KPC-producing K. pneumoniae isolates, with a polyclonal profile and the second by a dominant clone of colistin-resistant (≥ 32 μg/mL) KPC-producing K. pneumoniae. The cross transmission between patients became clear by the temporal and spatial relationships observed in the second outbreak, since some patients occupied the same bed, showing problems in hand hygiene adherence among healthcare workers and inadequate terminal disinfection of environment. The outbreak was contained when the ICU was closed to new admissions. Conclusions: The study showed an endemicity of K. pneumoniae KPC-producing in adult ICU, progressing to an epidemic monoclonal expansion, resulted by a very high antibiotic consumption of carbapenems and polymyxins and facilitated by failures in control measures the unit.

Uma proposta de ensino de frações no 6º ano do ensino fundamental a partir da teoria histórico-cultural

This text presents developed in the Graduate Program in Science and Mathematics Education at the Federal University of Uberlândia, in which it was intended to answer the question: What are the pedagogical implications for the fractions concept learning for students of the 6th grade of elementary school that the teaching guide activities can provide? The objectives of this research were: a) analyze the possible pedagogical implications for the learning of the fraction's concept for students of the 6th grade of elementary school through guiding teaching activities; b) using the conceptual connections of the fraction to enable students to develop an abstract thought and c) investigate whether guiding teaching activities reflect on 'how to think' and 'how to do' of the student. Five teaching activities have been developed (MOURA, 2002) from the perspective of teaching guiding activity (TGA) and had as object of study the teaching of fractions for students in 6th year of elementary school. They have been prepared and proposed activities in which it was intended to investigate the use of history of mathematics as an aid in learning the conceptual fraction links (CARAÇA, 1951) by students. Such activities, for analysis, were organized into episodes and scenes (MOURA, 2004) and discussed how students deal with the measurement of whole quantity (all) and subunits (part); how they represent in verbal or written language. It is hoped that the research is set up as an important contribution to mathematics teaching area and may contribute to the initial and continuing training of mathematics teacher sand the formation of theoretical thinking of elementary school students.