Note on the germination of Vochysia tucanorum seeds treated with growth regulators

(Note on the germination of Vochysia tucanorum seeds treated with growth regulators). The aim of this work was to evaluate the germination response of Vochysia tucanorum Mart. seeds treated with GA3 and CEPA and germinated under white light or darkness. Newly collected seeds from a Cerrado area were stored for 14 days at two temperatures (25 °C ± 2 and 7 °C ± 1). After the storage period the seeds were pre-treated with distilled water (control), gibberellic acid (GA3), 2-chloroethylphosphonic acid (CEPA) and a mixture of GA3 + CEPA. Following this, the seeds were sown in Petri dishes on filter paper moistened with distilled water and germinated in either darkness or white light. The results suggest that seeds are non-photoblastic and non-dormant, however a photoblastic behavior emerges when the seeds were previously stored at low temperature and imbibed in CEPA and GA3 solutions. In general, there is no difference between the 7 °C and 25 °C storage temperatures. The germination of seeds pre-treated with CEPA and CEPA + GA3 under white light was faster as compared to the distilled water control, and the effect of the CEPA + GA3 mixture was more pronounced than CEPA alone. Thus, the germination rate of V. tucanorum seeds can be improved by treatment with CEPA or CEPA + GA3 under white light.

Detection and analysis of apoptosis in peripheral blood cells from breast cancer patients

Apoptosis is a well-known specific process of cell death that normally occurs in physiological situations such as tissue or organ development and involution. During tumor growth there is a balance between proliferation and cell death which involves apoptotic mechanisms. In the present study genomic DNAs from 120 breast tumor biopsies were analyzed by agarose gel electrophoresis and none of them presented the fragmentation pattern characteristic of the apoptosis process. However, 33% of the 105 breast cancer patients clearly showed the apoptotic pattern when DNA from blood cells was analyzed. None of the DNAs from healthy volunteer blood cells showed any trace of apoptosis. Since the breast cancer patients were not receiving chemo- or hormone therapy, the possible relationship between blood cortisol levels and the apoptotic pattern found in patient blood cells was investigated. Using a chemoluminescence immunodetection assay, similar cortisol levels were observed in breast cancer patient sera presenting or not apoptotic blood cells and in healthy volunteer sera. Analysis of the clinical data obtained from 60 of these patients showed that patients bearing tumors of smaller size (under 20 mm) were more susceptible to the apoptotic effect in blood cells. According to the Elston grade, it was observed that 7 of 12 patients with grade III tumors (58%) presented apoptotic peripheral blood cells, in contrast to 10 of 48 patients with grade I and grade II tumors. These observations may reflect the immunosuppression characteristic of some breast cancer patients, which may contribute to tumor growth. Therefore, further studies are necessary to elucidate the factor(s) involved in such massive blood cell death.

Microsoftin pilvipalvelut pk-yrityksen päätelaite- ja tiedonhallinnassa

Diplomityössä tutkittiin Microsoftin pilvipalveluiden käyttöä pk-yrityksen päätelaitteiden hallinnassa sekä yrityksen tiedonhallinnassa. Kohdeyrityksenä oli pk-yritys, jolla on ollut Microsoftin pilvipalveluita käytössä jo useamman vuoden ajan. Pilvipalveluiden nopean päivitystahdin myötä uusimpien ominaisuuksien hyödyntäminen on suurelta osin jäänyt tekemättä. Tutkimus päädyttiin rajaamaan kohdeyrityksessä tehdyn vaatimusmäärittelyn mukaisiin tapauksiin, joihin pyrittiin etsimään vastauksia Microsoftin pilvipalveluiden tämän hetken ominaisuuksien avulla. Tutkimus toteutettiin tutustumalla kirjallisuuden avulla pilvitoimintamallin perusteisiin ja Microsoftin tarjoamiin pilvipalveluihin. Työn käytännön osuus toteutettiin useilla eri päätelaitteilla Office 365:den ja Windows Intunen muodostamassa testiympäristössä, jota laajennettiin Microsoft Azuren mahdollistamilla lisäominaisuuksilla. Pilvitoimintamallin yritykset taistelevat jatkuvasti käyttäjien luottamuksesta ja NSA:n vakoilutapauksen jälkeen palveluissa on tapahtunut paljon parannuksia. Tutkimuksessa todettiin erityisesti tiedon suojaamiskäytäntöjen ja hallintaominaisuuksien kehittyneen viime vuosina pilvitoimintamallin yritysten keskinäisen kilpailun myötä. Microsoftin pilvipalvelujen uudet ominaisuudet tarjoavat pk-yritysten käyttöön tehokkaita päätelaite- ja tiedonhallintaratkaisuja, joita on tähän asti ollut saatavilla vain suuryritysympäristöissä.

Quantitative Magnetic Resonance Imaging of the Liver and Heart In Iron Overload

Systemic iron overload (IO) is considered a principal determinant in the clinical outcome of different forms of IO and in allogeneic hematopoietic stem cell transplantation (alloSCT). However, indirect markers for iron do not provide exact quantification of iron burden, and the evidence of iron-induced adverse effects in hematological diseases has not been established. Hepatic iron concentration (HIC) has been found to represent systemic IO, which can be quantified safely with magnetic resonance imaging (MRI), based on enhanced transverse relaxation. The iron measurement methods by MRI are evolving. The aims of this study were to implement and optimise the methodology of non-invasive iron measurement with MRI to assess the degree and the role of IO in the patients. An MRI-based HIC method (M-HIC) and a transverse relaxation rate (R2*) from M-HIC images were validated. Thereafter, a transverse relaxation rate (R2) from spin-echo imaging was calibrated for IO assessment. Two analysis methods, visual grading and rSI, for a rapid IO grading from in-phase and out-of-phase images were introduced. Additionally, clinical iron indicators were evaluated. The degree of hepatic and cardiac iron in our study patients and IO as a prognostic factor in patients undergoing alloSCT were explored. In vivo and in vitro validations indicated that M-HIC and R2* are both accurate in the quantification of liver iron. R2 was a reliable method for HIC quantification and covered a wider HIC range than M-HIC and R2*. The grading of IO was able to be performed rapidly with the visual grading and rSI methods. Transfusion load was more accurate than plasma ferritin in predicting transfusional IO. In patients with hematological disorders, the prevalence of hepatic IO was frequent, opposite to cardiac IO. Patients with myelodysplastic syndrome were found to be the most susceptible to IO. Pre-transplant IO predicted severe infections during the early post-transplant period, in contrast to the reduced risk of graft-versus-host disease. Iron-induced, poor transplantation results are most likely to be mediated by severe infections.

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