Coupling between snoRNP assembly and 3' processing controls box C/D snoRNA biosynthesis in yeast

RNA polymerase II transcribes genes encoding proteins and a large number of small stable RNAs. While pre-mRNA 3'-end formation requires a machinery ensuring tight coupling between cleavage and polyadenylation, small RNAs utilize polyadenylation-independent pathways. In yeast, specific factors required for snRNA and snoRNA 3'-end formation were characterized as components of the APT complex that is associated with the core complex of the cleavage/polyadenylation machinery (core-CPF). Other essential factors were identified as independent components: Nrd1p, Nab3p and Sen1p. Here we report that mutations in the conserved box D of snoRNAs and in the snoRNP-specific factor Nop1p interfere with transcription and 3'-end formation of box C/D snoRNAs. We demonstrate that Nop1p is associated with box C/D snoRNA genes and that it interacts with APT components. These data suggest a mechanism of quality control in which efficient transcription and 3'-end formation occur only when nascent snoRNAs are successfully assembled into functional particles.

Spatial ecology of sooty owls in south-eastern Australian coastal forests : implications for forest management and reserve design

We investigated the home-range size and habitat use of eight Sooty Owls (Tyto tenebricosa tenebricosa) in coastal forests in East Gippsland, Victoria, Australia, between November 2006 and January 2008. The size of home-ranges varied widely; based on 95% adaptive kernel estimates, the average size of home-ranges of males was 3025ha (±1194s.d., n=3), whereas that of females was 994ha (±654s.d., n=5). Sooty Owls utilised a broad range of ecological vegetation classes and topographical features for roosting and foraging at a greater scale than previously assumed. There was minimal selection for habitat types based on floristic composition, primarily only avoiding heathlands (for foraging and roosting) and selecting particular dense foliage (rainforest and riparian scrub) for foliage roosting. Two Owls maintained home-ranges close to logged areas, with logging regrowth (<45 years old) being strongly avoided by both individuals. We recommend that the size of individual reserves for Sooty Owls in commercial forests should be increased to more closely resemble the core spatial resource requirements needed by a pair. Reserves should be largest where they feed predominantly on hollow-dependent prey. Most importantly, rather than conservation measures just focussing on the spatial requirements of Sooty Owls, efforts should be directed towards retaining high densities of crucial resources, such as hollow-bearing trees and mammalian prey species throughout the landscape.

Traditional environmental knowledge and community perceptions in sustaining the forest environment : case study of Minahasa ethnic community in the surrounding of Lake Tondano, North Sulawesi - Indonesia

The idea of sustainable development is distinct from the idea of restoring or conserving nature. This concept is embedded in the Rio Declaration on Environment and Development (1992) which Indonesia and several countries around the world have signed. Sustainable development seeks to interlace humans and nature, while restoration (especially at the large scale) often allows nature to be addressed separately, sometimes out of remorse for the damage caused by humans. In terms of attaining sustainable natural resource development, the opportunities offered by traditional ecological knowledge documentation are considered essential in enabling the achievement of sustainability because most of these Indigenous and/or local communities are situated in areas where many species have been historically cultivated and used in a sustainable way for thousands of years. The skill and techniques of these local communities can provide valuable information for the global community to evaluate current environmental policies. Such research and evaluation is often robustly and best undertaken through ethnoecological methodological paradigms. This paper examines the traditional environment knowledge of the Minahasan ethnic community, who live in the surrounds of Lake Tondano in the North Sulawesi, together with the Minahasan conscious and unconscious actions in conserving their forest ecology in addition to their knowledge of culture about forest protection in the region. In particular, contemporary use of traditional environmental knowledge is examined in terms of its relevance to in traditional resource management and land use planning, as avenues to better curate and manage natural resources through informed regional planning strategies and mechanisms.

Deeper than the forest AMBIENT

A short instrumental featuring percussion, bells, synths, mandolin, and sound design.

Deeper than the forest AMBIENT with VOCAL

A short instrumental featuring ethereal female vocals, percussion, bells, synths, mandolin, and sound design.

Ambient forest 30 sec

An ambient, new age style short composition featuring; African percussion and synths; 30 seconds in length.

Visual networking : keeping television on the box

The Australian National Broadband Network (NBN) is the largest infrastructure project ever proposed in Australia (NBN, 2010). Its Fibre to the Home open access network will see a new generation of telecommunications services providing the basis for technologies and services to be combined. Homes connected to the network will have access to new digital media and high-speed internet among other applications. Taking an Australian perspective, this paper focuses on the capacity for fast broadband to allow features and technologies to be combined that were once separate, but now have converged including computing, telephony, free-to-air (FTA) television, direct-to-home satellite broadcasting, radio, and the internet and the implications. Specifically, future services for digital television are going to be more akin to app-based functions that are currently available on mobiles and tablets but on the television screen rather than the PC. Against such a background, this article examines the future of television arguing that faster broadband and internet-enabled televisions to watch movies and shows when it suits the audience are the keys to the television’s survival.