Coupling between snoRNP assembly and 3' processing controls box C/D snoRNA biosynthesis in yeast
Data(s) |
16/06/2004
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Resumo |
RNA polymerase II transcribes genes encoding proteins and a large number of small stable RNAs. While pre-mRNA 3'-end formation requires a machinery ensuring tight coupling between cleavage and polyadenylation, small RNAs utilize polyadenylation-independent pathways. In yeast, specific factors required for snRNA and snoRNA 3'-end formation were characterized as components of the APT complex that is associated with the core complex of the cleavage/polyadenylation machinery (core-CPF). Other essential factors were identified as independent components: Nrd1p, Nab3p and Sen1p. Here we report that mutations in the conserved box D of snoRNAs and in the snoRNP-specific factor Nop1p interfere with transcription and 3'-end formation of box C/D snoRNAs. We demonstrate that Nop1p is associated with box C/D snoRNA genes and that it interacts with APT components. These data suggest a mechanism of quality control in which efficient transcription and 3'-end formation occur only when nascent snoRNAs are successfully assembled into functional particles.<br /> |
Identificador | |
Idioma(s) |
eng |
Publicador |
Nature Publishing Group |
Relação |
http://dro.deakin.edu.au/eserv/DU:30039286/dichtl-couplingbetween-2004.pdf http://dx.doi.org/10.1038/sj.emboj.7600254 |
Direitos |
2004, Nature Publishing Group |
Palavras-Chave | #3'-end formation #Nop1p #Saccharomyces cerevisiae #snoRNA #snoRNP assembly |
Tipo |
Journal Article |